Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library. application to the detection of mycobacteria

ABSTRACT

The present invention is directed to a method for isolating a polynucleotide of interest that is present or is expressed in a genome of a first mycobacterium strain and that is absent or altered in a genome of a second mycobacterium strain which is different from the first mycobacterium strain using a bacterial artificial chromosome (BAC) vector. The invention further relates to a polynucleotide isolated by this method and recombinant BAC vector used in this method. In addition the present invention comprises method and kit for detecting the presence of a mycobacteria in a biological sample.

This application is a division of U.S. application Ser. No. 09/673,476, filed Nov. 30, 2000, now U.S. Pat. No. 7,112,663 which is a national stage application of PCT/IB99/00740, filed Apr. 16, 1999, which is a continuation of U.S. application Ser. No. 09/060,756, filed Apr. 16, 1998, now U.S. Pat. No. 6,183,957, all of which are incorporated herein by reference.

SEQUENCE LISTING

The instant application contains a “lengthy” Sequence Listing, which has been submitted via CD-R in lieu of a printed paper copy, and is hereby incorporated by reference in its entirety. Said CD-R, recorded on Nov. 30, 2006, are labeled CRF, “Copy 1” and “Copy 2”, respectively, and each contains only one identical 524 Kb file (34950320.APP).

I. BACKGROUND OF THE INVENTION

The present invention pertains to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobacterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC). The invention concerns also polynucleotides identified by the above method, as well as detection methods for mycobacteria, particularly Mycobacterium tuberculosis, and kits using said polynucleotides as primers or probes. Finally, the invention deals with BAC-based mycobacterium DNA libraries used in the method according to the invention and particularly BAC-based Mycobacterium tuberculosis and Mycobacterium bovis BCG DNA libraries.

Radical measures are required to prevent the grim predictions of the World Health Organisation for the evolution of the global tuberculosis epidemic in the next century becoming a tragic reality. The powerful combination of genomics and bioinformatics is providing a wealth of information about the etiologic agent, Mycobacterium tuberculosis, that will facilitate the conception and development of new therapies. The start point for genome sequencing was the integrated map of the 4.4 Mb circular chromosome of the widely-used, virulent reference strain, M. tuberculosis H37Rv and appropriate cosmids were subjected to systematic shotgun sequence analysis at the Sanger Centre.

Cosmid clones (Balasubramanian et al., 1996; Pavelka et al., 1996) have played a crucial role in the M. tuberculosis H37Rv genome sequencing project. However, problems such as under-representation of certain regions of the chromosome, unstable inserts and the relatively small insert size complicated the production of a comprehensive set of canonical cosmids representing the entire genome.

II. SUMMARY OF THE INVENTION

In order to avoid the numerous technical constraints encountered in the state of the art, as described hereabove, when using genomic mycobacterial DNA libraries constructed in cosmid clones, the inventors have attempted to realize genomic mycobacterial DNA libraries in an alternative type of vectors, namely Bacterial Artificial Chromosome (BAC) vectors.

The success of this approach depended on whether the resulting BAC clones could maintain large mycobacterial DNA inserts. There are various reports describing the successful construction of a BAC library for eucaryotic organisms (Cai et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997) where inserts up to 725 kb (Zimmer et al., 1997) were cloned and stably maintained in the E. coli host strain.

Here, it is shown that, surprisingly, the BAC system can also be used for mycobacterial DNA, as 70% of the clones contained inserts in the size of 25 to 104 kb.

This is the first time that bacterial, and specifically mycobacterial, DNA is cloned in such BAC vectors.

In an attempt to obtain complete coverage of the genome with a minimal overlapping set of clones, a Bacterial Artificial Chromosome (BAC) library of M. tuberculosis was constructed, using the vector pBeloBAC11 (Kim et al., 1996) which combines a simple phenotypic screen for recombinant clones with the stable propagation of large inserts (Shizuya et al., 1992). The BAC cloning system is based on the E. coli F-factor, whose replication is strictly controlled and thus ensures stable maintenance of large constructs (Willets et al., 1987). BACs have been widely used for cloning of DNA from various eucaryotic species (Cai et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997). In contrast, to our knowledge this report describes the first attempt to use the BAC system for cloning bacterial DNA.

A central advantage of the BAC cloning system over cosmid vectors used in prior art is that the F-plasmid is present in only one or a maximum of two copies per cell, reducing the potential for recombination between DNA fragments and, more importantly, avoiding the lethal overexpression of cloned bacterial genes. However, the presence of the BAC as just a single copy means that plasmid DNA has to be extracted from a large volume of culture to obtain sufficient DNA for sequencing and it is described here in the examples a simplified protocol to achieve this.

Further, the stability and fidelity of maintenance of the clones in the BAC library represent ideal characteristics for the identification of genomic differences possibly responsible for phenotypic variations in different mycobacterial species.

As it will be shown herein, BACs can be allied with conventional hybridization techniques for refined analyses of genomes and transcriptional activity from different mycobacterial species.

Having established a reliable procedure to screen for genomic polymorphisms, it is now possible to conduct these comparisons on a more systematic basis than in prior art using representative BACs throughout the chromosome and genomic DNA from a variety of mycobacterial species.

As another approach to display genomic polymorphisms, the inventors have also started to use selected H37Rv BACs for “molecular combing” experiments in combination with fluorescent in situ hybridization (Bensimon et al., 1994; Michalet et al., 1997). With such techniques the one skilled in the art is enabled to explore the genome of mycobacteria in general and of M. tuberculosis in particular for further polymorphic regions.

The availability of BAC-based genomic mycobacterial DNA libraries constructed by the inventors have allowed them to design methods and means both useful to identify genomic regions of interest of pathogenic mycobacteria, such as Mycobacterium tuberculosis, that have no counterpart in the corresponding non-pathogenic strains, such as Mycobacterium bovis BCG, and useful to detect the presence of polynucleotides belonging to a specific mycobacterium strain in a biological sample.

By a biological sample according to the present invention, it is notably intended a biological fluid, such as plasma, blood, urine or saliva, or a tissue, such as a biopsy.

Thus, a first object of the invention consists of a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobacterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).

The invention is also directed to a polynucleotide of interest that has been isolated according to the above method and in particular a polynucleotide containing one or several Open Reading Frames (ORFs), for example ORFs encoding either a polypeptide involved in the pathogenicity of a mycobacterium strain or ORFs encoding Polymorphic Glycine Rich Sequences (PGRS).

Such polynucleotides of interest may serve as probes or primers in order to detect the presence of a specific mycobacterium strain in a biological sample or to detect the expression of specific genes in a particular mycobacterial strain of interest.

The BAC-based genomic mycobacterial DNA libraries generated by the present inventors are also part of the invention, as well as each of the recombinant BAC clones and the DNA insert contained in each of said recombinant BAC clones.

The invention also pertains to methods and kits for detecting a specific mycobacterium in a biological sample using either at least one recombinant BAC clone or at least one polynucleotide according to the invention, as well as to methods and kits to detect the expression of one or several specific genes of a given mycobacterial strain present in a biological sample.

III. BRIEF DESCRIPTION OF THE FIGURES

In order to better understand the present invention, reference will be made to the appended figures which depicted specific embodiments to which the present invention is in no case limited in scope with.

FIGS. 1A and 1B: PCR-screening for unique BAC clones with specific primers for 2 selected genomic regions of the H37Rv chromosome, using 21 pools representing 2016 BACs (FIG. 1A) and sets of 20 subpools from selected positive pools (FIG. 1B).

FIG. 2: Pulsed-field gel electrophoresis gel of DraI-cleaved BAC clones used for estimating the insert sizes of BACs.

FIG. 3: Minimal overlapping BAC map of M. tuberculosis H37Rv superimposed on the integrated physical and genetic map established by Philipp et al. (18). Y- and I-numbers show pYUB328 (2) and pYUB412 (16) cosmids which were shotgun sequenced during the H37Rv genome sequencing project. Y-cosmids marked with * were shown in the integrated physical and genetic map (18). Rv numbers show the position of representative BAC clones relative to sequenced Y- and I-clones. Squared Rv numbers show BACs which were shotgun sequenced at the Sanger Centre.

FIGS. 4A and 4B: Ethidium bromide stained gel (FIG. 4A) and corresponding Southern blot (FIG. 4B) of EcoRI and PvuII digested Rv58 DNA hybridized with ³²P labeled genomic DNA preparations from M. tuberculosis H37Rv, M. bovis ATCC 19210 and M. bovis BCG Pasteur.

FIG. 5: Organisation of the ORFs in the 12.7 kb genomic region present in M. tuberculosis H37Rv but not present in M. bovis ATCC 19210 and M. bovis BCG Pasteur. Arrows show the direction of transcription of the putative genes. Positions of EcoRI and PvuII restriction sites are shown. Vertical dashes represent stop codons. The 11 ORFs correspond to the ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library. The junction sequences flanking the polymorphic region are shown (SEQ ID NOS 754 & 755 are disclosed, respectively, in order of appearance).

FIG. 6: Variation in the C-terminal part of a PE-PGRS open reading frame in M. tuberculosis strain H37Rv (SEQ ID NO: 727) relative to M. bovis BCG strain Pasteur (SEQ ID NO: 728).

The numbers on the right side of the Figure denote the position of the end nucleotides, taking as the reference the M. tuberculosis genome.

FIG. 7: Polynucleotide sequence next to the HindIII cloning site in the BAC vector pBeloBAC11 (Kim et al., 1996) used to clone the inserts of the BAC-based mycobacterial genomic DNA library according to the invention (SEQ ID NO: 29).

NotI: location of the NotI restriction sites.

Primer T7-BAC1: nucleotide region recognized by the T7-BAC1 primer shown in Table 1.

T7 promoter: location of the T7 promoter region on the pBeloBac11 vector.

Primer T7-Belo2: nucleotide region recognized by the T7-Belo2 primer shown in Table 1.

HindIII: the HindIII cloning site used to clone the genomic inserts in the pBeloBAC11 vector.

SP6-Mid primer: nucleotide region recognized by the SP6 Mid primer shown in Table 1.

SP6-BAC1 primer: nucleotide region recognized by the SP6 BAC1 primer shown in Table 1.

SP6 promoter: location of the SP6 promoter region on the pBeloBac11 vector.

IV. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

As already mentioned hereinbefore, the present invention is directed to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobacterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC) type vector.

For this purpose, the inventors have constructed several BAC-based mycobacterial genomic DNA libraries that may be used in order to perform the above described method.

Because it is the first time that mycobacterial genomic, DNA has been successfully cloned in BAC type vectors, and because these DNA libraries are then novel and nonobvious, an object of the present invention consists in a mycobacterial genomic DNA library cloned in such a BAC type vector.

As an illustrative example, a BAC-based DNA library of Mycobacterium tuberculosis has been realized. Forty-seven cosmids chosen from the integrated map of the 4.4 Mb circular chromosome (Philipp et al., 1996a) were shotgun-sequenced during the initial phase of the H37Rv genome sequence project. The sequences of these clones were used as landmarks in the construction of a minimally overlapping BAC map. Comparison of the sequence data from the termini of 420 BAC clones allowed us to establish a minimal overlapping BAC map and to fill in the existing gaps between the sequence of cosmids. As well as using the BAC library for genomic mapping and sequencing, we also tested the system in comparative genomic experiments in order to uncover differences between two closely related mycobacterial species. As shown in a previous study (Philipp et al., 1996b), M. tuberculosis, M. bovis and M. bovis BCG, specifically BCG Pasteur strain, exhibit a high level of global genomic conservation, but certain polymorphic regions were also detected. Therefore, it was of great interest to find a reliable, easy and rapid way to exactly localize polymorphic regions in mycobacterial genomes using selected BAC clones. This approach was validated by determining the exact size and location of the polymorphisms in the genomic region of DraI fragment Z4 (Philipp et al., 1996b), taking advantage of the availability of an appropriate BAC clone covering the polymorphic region and the H37Rv genome sequence data. This region is located approximately 1.7 Mb from the origin of replication.

The Bacterial Artificial Chromosome (BAC) cloning system is capable of stably propagating large, complex DNA inserts in Escherichia coli. As part of the Mycobacterium tuberculosis H37Rv genome sequencing project, a BAC library was constructed in the pBeloBAC11 vector and used for genome mapping, confirmation of sequence assembly, and sequencing. The library contains about 5000 BAC clones, with inserts ranging in size from 25 to 104 kb, representing theoretically a 70 fold coverage of the M. tuberculosis genome (4.4 Mb). A total of 840 sequences from the T7 and SP6 termini of 420 BACs were determined and compared to those of a partial genomic database. These sequences showed excellent correlation between the estimated sizes and positions of the BAC clones and the sizes and positions of previously sequenced cosmids and the resulting contigs. Many BAC clones represent linking clones between sequenced cosmids, allowing full coverage of the H37Rv chromosome, and they are now being shotgun-sequenced in the framework of the H37Rv sequencing project. Also, no chimeric, deleted or rearranged BAC clones were detected, which was of major importance for the correct mapping and assembly of the H37Rv sequence. The minimal overlapping set contains 68 unique BAC clones and spans the whole H37Rv chromosome with the exception of a single gap of ˜150 kb. As a post-genomic application, the canonical BAC set was used in a comparative study to reveal chromosomal polymorphisms between M. tuberculosis, M. bovis and M. bovis BCG Pasteur, and a novel 12.7 kb segment present M. tuberculosis but absent from M. bovis and M. bovis BCG was characterized. This region contains a set of genes whose products show low similarity to proteins involved in polysaccharide biosynthesis. The H37Rv BAC library therefore provides the one skilled in the art with a powerful tool both for the generation and confirmation of sequence data as well as for comparative genomics and a plurality of post-genomic applications.

The above described BAC-based Mycobacterium tuberculosis genomic DNA library is part of the present invention and has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on Nov. 19, 1997 under the accession number 1-1945.

Another BAC-based DNA library has been constructed with the genomic DNA of Mycobacterium bovis BCG, Pasteur strain, and said DNA library has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on Jun. 30, 1998 under the accession number I-2049.

Thus, as a specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library that has been constructed from the genomic DNA of Mycobacterium tuberculosis, more specifically of the H37Rv strain and particularly of the DNA library deposited in the accession number 1-1945.

In another specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library has been constructed from the genomic DNA of Mycobacterium bovis BCG, more specifically of the Pasteur strain and particularly of the DNA library deposited in the accession number I-2049.

In more details, the method according to the invention for isolating a polynucleotide of interest may comprise the following steps:

a) isolating at least one polynucleotide contained in a clone of a BAC-based DNA library of mycobacterial origin;

b) isolating:

-   -   at least one genomic or cDNA polynucleotide from a         mycobacterium, said mycobacterium belonging to a strain         different from the strain used to construct the BAC-based DNA         library of step a); or alternatively     -   at least one polynucleotide contained in a clone of a BAC-based         DNA library prepared from the genome of a mycobacterium that is         different from the mycobacterium used to construct the BAC-based         DNA library of step a);

c) hybridizing the at least one polynucleotide of step a) to the at least one polynucleotide of step b);

d) selecting the at least one polynucleotide of step a) that has not formed a hybrid complex with the at least one polynucleotide of step b);

e) characterizing the selected polynucleotide.

Following the above procedure, the at least one polynucleotide of step a) may be prepared as follows:

1) digesting at least one recombinant BAC clone by an appropriate restriction endonuclease in order to isolate the polynucleotide insert of interest from the vector genetic material;

2) optionally amplifying the resulting polynucleotide insert;

3) optionally digesting the polynucleotide insert of step 1) or step 2) with at least one restriction endonuclease.

The above method of the invention allows the one skilled in the art to perform comparative genomics between different strains or species of mycobacteria cells, for example between pathogenic strains or species and their non pathogenic strains or species counterparts, as it is the illustrative case for the genomic comparison between Mycobacterium tuberculosis and Mycobacterium bovis BCG that is described herein in the examples.

Restriction digests of a given clone of a BAC library according to the invention may be blotted to membranes, and then probed with radiolabeled DNA form another strain or another species of mycobacteria, allowing the one skilled in the art to identify, characterize and isolate a polynucleotide of interest that may be involved in important metabolical and/or physiological pathways of the mycobacterium under testing, such as a polynucleotide functionally involved in the pathogenicity of said given mycobacteria for its host organism.

More specifically, the inventors have shown in Example 6 that when restriction digests of a given clone of the BAC library identified by the CNCM accession number 1-1945 are blotted to membranes and then probed with radiolabeled total genomic DNA from, for example, Mycobacterium bovis BCG Pasteur, it is observed that restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA are absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv.

Thus, a further object of the present invention consists in a polynucleotide of interest that has been isolated according to the method described herein before.

In Example 6, a polynucleotide of approximately 12.7 kilobases has been isolated that is present in the genome of M. tuberculosis but is absent of the genome of M. bovis BCG. This polynucleotide of interest contains 11 ORFs that may be involved in polysaccharide biosynthesis. In particular, two of said ORFs are of particular interest namely ORF6 (MTCY277.33; Rv1511) that encodes a protein that shares significant homology with bacterial GDP-D-mannose dehydratases, whereas the protein encoded by ORF7 (MTCY277.34; Rv1512) shares significant homology with a nucleotide sugar epimerase. As polysaccharide is a major constituent of the mycobacterial cell wall, these deleted genes may cause the cell wall of M. bovis BCG to differ from that of M. tuberculosis, a fact that may have important consequences for both the immune response to M. bovis BCG and virulence. Detection of such a polysaccharide is of diagnostic interest and possibly useful in the design of tuberculosis vaccines.

Consequently, the polynucleotide of interest obtained following the method according to the invention may contain at least one ORF, said ORF preferably encoding all or part of a polypeptide involved in an important metabolical and/or physiological pathway of the mycobacteria under testing, and more specifically all or part of a polypeptide that is involved in the pathogenicity of the mycobacteria under testing, such as for example Mycobacterium tuberculosis, and more generally mycobacteria belonging to the Mycobacterium tuberculosis complex.

The Mycobacterium tuberculosis complex has its usual meaning, i.e. the complex of mycobacteria causing tuberculosis which are Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti and the vaccine strain Mycobacterium bovis BCG.

An illustrative polynucleotide of interest according to the present invention comprises all or part of the polynucleotide of approximately 12.7 kilobases that is present in the genome of M. tuberculosis but is absent from the genome of M. bovis BCG disclosed hereinbefore. This polynucleotide is contained in clone Rv58 of the BAC DNA library I-1945.

Generally, the invention also pertains to a purified polynucleotide comprising the DNA insert contained in a recombinant BAC vector belonging to a BAC-based mycobacterial genomic DNA library, such as for example the I-1945 BAC DNA library.

Advantageously, such a polynucleotide has been identified according to the method of the invention.

Such a polynucleotide of interest may be used as a probe or a primer useful for specifically detecting a given mycobacterium of interest, such as Mycobacterium tuberculosis or Mycobacterium bovis BCG.

More specifically, the invention then deals with a purified polynucleotide useful as probe or a primer comprising all or part of the nucleotide sequence SEQ ID No 1.

The location, on the Mycobacterium tuberculosis chromosome, of the above polynucleotide of sequence SEQ ID No 1 has now been ascribed to begin, at its 5′ end at nucleotide at position nt 1696015 and to end, at its 3′ end, at nucleotide at position nt 1708746.

For diagnostic purposes, this 12.7 kb deletion should allow a rapid PCR screening of tubercle isolates to identify whether they are bovine or human strains. The primers listed in Table I are flanking the deleted region and give a 722 bp amplicon in M. bovis or M. bovis BCG strains, but a fragment of 13,453 bp in M. tuberculosis that is practically impossible to amplify under the same PCR conditions. More importantly, assuming that some of the gene products from this region represent proteins with antigenic properties, it could be possible to develop a test that can reliably distinguish between the immune response induced by vaccination with M. bovis BCG vaccine strains and infection with M. tuberculosis or that the products (e.g. polysaccharides) are specific immunogens.

The invention also provides for a purified polynucleotide useful as a probe or as a primer, said polynucleotide being chosen in the following group of polynucleotides:

a) a polynucleotide comprising at least 8 consecutive nucleotides of the sequence SEQ ID No 1;

b) a polynucleotide whose sequence is fully complementary to the sequence of the polynucleotide defined in a);

c) a polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).

For the purpose of defining a polynucleotide or oligonucleotide hybridizing under stringent hybridization conditions, such as above, it is intended a polynucleotide that hybridizes with a reference polynucleotide under the following hybridization conditions.

The hybridization step is realized at 65° C. in the presence of 6×SSC buffer, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml of salmon sperm DNA.

For technical information, 1×SSC corresponds to 0.15 M NaCl and 0.05M sodium citrate; 1×Denhardt's solution corresponds to 0.02% Ficoll, 0.02% polyvinylpyrrolidone and 0.02% bovine serum albumin.

The hybridization step is followed by four washing steps

-   -   two washings during 5 min, preferably at 65° C. in a 2×SSC and         0.1% SDS buffer,     -   one washing during 30 min, preferably at 65° C. in a 2×SSC and         0.1% SDS buffer,     -   one washing during 10 min, preferably at 65° C. in a 0.1×SSC and         0.1% SDS buffer.

A first illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID No 1 is the polynucleotide of sequence SEQ ID No 2 that corresponds to the Sp6 end-sequence of SEQ ID No 1.

A second illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID No 1 is the polynucleotide of sequence SEQ ID No 3 that corresponds to the T7 end-sequence of SEQ ID No 1, located on the opposite strand.

The polynucleotide of sequence SEQ ID No 1 contains 11 ORFs, the respective locations of which, taking into account the orientation of each ORF on the chromosome, on the sequence of the Mycobacterium tuberculosis chromosome, is given hereafter:

-   -   The location of ORF1 is comprised between nucleotide at position         nt 1695944 and nucleotide at position nt 1696441.

The location of ORF2 is comprised between nucleotide at position nt 1696728 and nucleotide at position nt1697420 (SEQ ID NO: 744).

The location of ORF3 is comprised between nucleotide at position nt 1698096 and nucleotide at position nt1699892. ORF3 probably encodes a protein having the characteristics of a membrane protein (SEQ ID NO: 745).

The location of ORF4 is comprised between nucleotide at position nt 1700210 and nucleotide at position nt1701088 (SEQ ID NO: 746).

The location of ORF5 is comprised between nucleotide at position nt 1701293 and nucleotide at position nt1702588. ORF5 encodes a protein having the characteristics of a membrane protein (SEQ ID NO: 747).

The location of ORF6 is comprised between nucleotide at position nt 1703072 and nucleotide at position nt1704091. ORF6 encodes a protein having the characteristics of a GDP-D-mannose dehydratase (SEQ ID NO: 748).

The location of ORF7 is comprised between nucleotide at position nt 1704091 and nucleotide at position nt1705056. ORF7 encodes a protein having the characteristics of a nucleotide sugar epimerase involved in colanic acid biosynthesis (SEQ ID NO: 749).

The location of ORF8 is comprised between nucleotide at position nt 1705056 and nucleotide at position nt1705784 (SEQ ID NO: 750).

The location of ORF9 is comprised between nucleotide at position nt 1705808 and nucleotide at position nt1706593. ORF9 encodes a protein having the characteristics of colanic acid biosynthesis glycosyl transferase (SEQ ID NO: 751).

The location of ORF10 is comprised between nucleotide at position nt 1706631 and nucleotide at position nt 1707524 (SEQ ID NO: 752).

The location of ORF11 is comprised between nucleotide at position nt 1707530 and nucleotide at position nt 1708648. ORF11 encodes a protein similar to a spore coat polysaccharide biosynthesis (SEQ ID NO: 753).

A polynucleotide of interest obtained by the above-disclosed method according to the invention may also contain at least one ORF that encodes all or part of acidic, glycine-rich proteins, belonging to the PE and PPE families, whose genes are often clustered and based on multiple copies of the polymorphic repetitive sequences. The names PE and PPE derive from the fact that the motifs ProGlu (PE, positions 8, 9) and ProProGlu (PPE, positions 7 to 9) are found near the N-terminus in almost all cases. The PE protein family all have a highly conserved N-terminal domain of ˜110 amino acid residues, that is predicted to have a globular structure, followed by a C-terminal segment which varies in size, sequence and repeat copy number. Phylogenetic analysis separated the PE family into several groups, the larger of which is the highly repetitive PGRS class containing 55 members whereas the other groups share very limited sequence similarity in their C-terminal domains. The predicted molecular weights of the PE proteins vary considerably as a few members only contain the ˜110 amino acid N-terminal domain while the majority have C-terminal extensions ranging in size from 100 up to >1400 residues. A striking feature of the PGRS proteins is their exceptional glycine content (up to 50%) due to the presence of multiple tandem repetitions of GlyGlyAla or GlyGlyAsn motifs or variations thereof.

Like the PE family, the PPE protein family also has a conserved N-terminal domain that comprises ˜180 amino acid residues followed by C-terminal segments that vary considerably in sequence and length. These proteins fall into at least three groups, one of which constitutes the MPTR class characterised by the presence of multiple, tandem copies of the motif AsnXGlyXGlyAsnXGly (SEQ ID NO. 730). The second subgroup contains a characteristic, well-conserved motif around position 350 (GlyXXSerValProXXTrp)(SEQ ID NO. 731), whereas the other group contains proteins that are unrelated except for the presence of the common 180-residue PPE domain. C-terminal extensions may range in size from 00 up to 3500 residues.

One member of the PGRS sub-family, the WHO antigen 22T (Abou-Zeid et al., 1991), a 55 kD protein capable of binding fibronectin, is produced during disease and elicits a variable antibody response suggesting either that individuals mount different immune responses or that this PGRS-protein may not be produced in this form by all strains of M. tuberculosis. In other words, at least some PE_PGRS coding sequences encode for proteins that are involved in the recognition of M. tuberculosis by the immune system of the infected host. Therefore, differences in the PGRS sequences could represent the principal source of antigenic variation in the otherwise genetically and antigenically homogeneous bacterium.

By performing the method of the invention using the M. tuberculosis BAC based DNA library I-1945, the inventors have discovered the occurrence of sequence differences between a given PGRS encoding ORF (ORF reference on the genomic sequence of M. tuberculosis Rv0746) of M. tuberculosis and its counterpart sequence in the genome of M. bovis BCG.

More precisely, the inventors have determined that one ORF contained in BAC vector No Rv418 of the M. tuberculosis BCG I-1945 DNA library carries both base additions and base deletions when compared with the corresponding ORF in the genome of M. bovis BCG that is contained in the BAC vector No X0175 of the M. bovis BCG I-2049 DNA library. The variations observed in the base sequences correspond to variations in the C-terminal part of the amino acid sequence of the PGRS ORF translation product.

As shown in FIG. 6, an amino acid stretch of 9 residues in length is present in this M. tuberculosis PGRS (ORf reference Rv0746) and is absent from the ORF counterpart of M. bovis BCG, namely the following amino acid sequence:

(SEQ ID NO. 732) NH₂-GGAGGAGGSSAGGGGAGGAGGAGGWLL GD-COOH.

Furthermore, FIG. 6 shows also that an amino acid stretch of 45 residues in length is absent from this M. tuberculosis PGRS and is present in the ORF counterpart of M. bovis BCG, namely following amino acid sequence:

(SEQ ID NO. 733) NH₂-GAGGIGGIGGNANGGAGGNGGTGGQLWGSGGAGVEGGAAL SVGDT-COOH.

Similar observations were made with PPE ORF Rv0442, which showed a 5 codon deletion relative to a M. bovis amino acid sequence.

Given that the polymorphism associated with the PE-PGRS or PEE ORFS resulted in extensive antigenic variability or reduced antigen presentation, this would be of immense significance for vaccine design, for understanding protective immunity in tuberculosis and, possibly, explain the varied responses seen in different BCG vaccination programmes.

There are several striking parallels between the PGRS proteins and the Epstein-Barr virus-encoded nuclear antigens (EBNA). Both polypeptide families are glycine-rich, contain Gly-Ala repeats that represent more than one third of the molecule, and display variation in the length of the repeat region between different isolates. The Gly-Ala repeat region of EBNA1 has been shown to function as a cis-acting inhibitor of antigen processing and MHC class I-restricted antigen presentation. (Levitskaya et al., 1995). The fact that MHC class I knock-out mice are extremely susceptible to M. tuberculosis underlines the importance of MHC class I antigen presentation in protection against tuberculosis. Therefore, it is possible that the PE/PPE protein family also play some role in inhibiting antigen presentation, allowing the bacillus to hide from the host's immune system.

As such the novel and nonobvious PGRS polynucleotide from M. bovis which is homolog to the M. tuberculosis ORF Rv0746, and which is contained in the BAC clone No X0175 (See Table 4 for SP6 and T7 end-sequences of clone no X0175) of the I-2049 M. bovis BCG BAC DNA library is part of the present invention, as it represents a starting material in order to define specific probes or primers useful for detection of antigenic variability in mycobacterial strains, possible inhibition of antigen processing as well as to differentiate M. tuberculosis from M. bovis BCG.

Thus, a further object of the invention consists in a polynucleotide comprising the sequence SEQ ID No 4.

Polynucleotides of interest have been defined by the inventors as useful detection tools in order to differentiate M. tuberculosis from M. bovis BCG. Such polynucleotides are contained in the 45 amino acid length coding sequence that is present in M. bovis BCG but absent from M. tuberculosis. This polynucleotide has a sequence beginning (5′ end) at the nucleotide at position nt 729 of the sequence SEQ ID No 4 and ending (3′ end) at the nucleotide in position nt 863 of the sequence SEQ ID No 4.

Thus, part of the present invention is also a polynucleotide which is chosen among the following group of polynucleotides:

a) a polynucleotide comprising at least 8 consecutive nucleotides of the nucleotide sequence SEQ ID No 5;

b) a polynucleotide which sequence is fully complementary to the sequence of the polynucleotide defined in a);

c) a polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).

The stringent hybridization conditions for the purpose of defining the above disclosed polynucleotide are defined herein before in the specification.

The invention also provides for a BAC-based Mycobacterium tuberculosis strain H37Rv genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on Nov. 19, 1997 under the accession number I-1945.

A further object of the invention consists in a recombinant BAC vector which is chosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-1945.

Generally, a recombinant BAC vector of interest may be chosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-1945:

Rv101; Rv102; Rv103; Rv104; Rv105; Rv106; Rv107; Rv108; Rv109; Rv10; Rv110; Rv111; Rv112; Rv113; Rv114; Rv115; Rv116; Rv117; Rv118; Rv119; Rv11; Rv120; Rv121; Rv122; Rv123; Rv124; Rv126; Rv127; Rv128; Rv129; Rv130; Rv132; Rv134; Rv135; Rv136; Rv137; Rv138; Rv139; Rv13; Rv140; Rv141; Rv142; Rv143; Rv144; Rv145; Rv146; Rv147; Rv148; Rv149; Rv14; Rv150; Rv151; Rv152; Rv153; Rv154; Rv155; Rv156; Rv157; Rv159; Rv15; Rv160; Rv161; Rv162; Rv163; Rv164; Rv165; Rv166; Rv167; Rv169; Rv16; Rv170; Rv171; Rv172; Rv173; Rv174; Rv175; Rv176; Rv177; Rv178; Rv179; Rv17; Rv180; Rv181; Rv182; Rv183; Rv184; Rv185; Rv186; Rv187; Rv188; Rv18; Rv190; Rv191; Rv192; Rv193; Rv194; Rv195; Rv196; Rv19; Rv1; Rv201; Rv204; Rv205; Rv207; Rv209; Rv20; Rv214; Rv215; Rv217; Rv218; Rv219; Rv21; Rv220; Rv221; Rv222; Rv223; Rv224; Rv225; Rv226; Rv227; Rv228; Rv229; Rv22; Rv230; Rv231; Rv232; Rv233; Rv234; Rv235; Rv237; Rv240; Rv241; Rv243; Rv244; Rv245; Rv246; Rv247; Rv249; Rv24; Rv251; Rv252; Rv253; Rv254; Rv255; Rv257; Rv258; Rv259; Rv25; Rv260; Rv261; Rv262; Rv263; Rv264; Rv265; Rv266; Rv267; Rv268; Rv269; Rv26; Rv270; Rv271; Rv272; Rv273; Rv274; Rv275; Rv276; Rv277; Rv278; Rv279; Rv27; Rv280; Rv281; Rv282; Rv283; Rv284; Rv285; Rv286; Rv287; Rv288; Rv289; Rv28; Rv290; Rv291; Rv292; Rv293; Rv294; Rv295; Rv296; Rv29; Rv2; Rv301; Rv302; Rv303; Rv304; Rv306; Rv307; Rv308; Rv309; Rv30; Rv310; Rv31; Rv312; Rv313; Rv314; Rv315; Rv316; Rv317; Rv318; Rv319; Rv31; Rv32; Rv322; Rv327; Rv328; Rv329; Rv32; Rv330; Rv331; Rv333; Rv334; Rv335; Rv336; Rv337; Rv338; Rv339; Rv33; Rv340; Rv341; Rv343; Rv344; Rv346; Rv347; Rv348; Rv349; Rv34; Rv350; Rv351; Rv352; Rv353; Rv354; Rv355; Rv356; Rv357; Rv358; Rv359; Rv35; Rv360; Rv361; Rv363; Rv364; Rv365; Rv366; Rv367; Rv368; Rv369; Rv36; Rv370; Rv371; Rv373; Rv374; Rv375; Rv376; Rv377; Rv378; Rv379; Rv37; Rv381; Rv382; Rv383; Rv384; Rv385; Rv386; Rv387; Rv388; Rv389; Rv38; Rv390; Rv391; Rv392; Rv393; Rv396; Rv39; Rv3; Rv40; Rv412; Rv413; Rv414; Rv415; Rv416; Rv417; Rv418; Rv419; Rv41; Rv42; Rv43; Rv44; Rv45; Rv46; Rv47; Rv48; Rv49; Rv4; Rv50; Rv51; Rv52; Rv53; Rv54; Rv55; Rv56; Rv57; Rv58; Rv59; Rv5; Rv60; Rv61; Rv62; Rv63; Rv64; Rv65; Rv66; Rv67; Rv68; Rv69; Rv6; Rv70; Rv71; Rv72; Rv73; Rv74; Rv75; Rv76; Rv77; Rv78; Rv79; Rv7; Rv80; Rv81; Rv82; Rv83; Rv84; Rv85; Rv86; Rv87; Rv88; Rv89; Rv8; Rv90; Rv91; Rv92; Rv94; Rv95; Rv96; Rv9.

The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 3.

It has been shown by the inventors that the minimal overlapping set of BAC vectors of the BAC-based DNA library I-1945 contains 68 unique BAC clones and practically spans almost the whole H37Rv chromosome with the exception of a single gap of approximately 150 kb.

More specifically, a recombinant BAC vector of interest is chosen among the following set or group of BAC vectors from the BAC-based DNA library I-1945, the location of which vector DNA inserts on the chromosome of M. tuberculosis is shown in FIG. 3:

Rv234; Rv351; Rv166; Rv35; Rv415; Rv404; Rv209; Rv272; Rv30; Rv228; Rv233; Rv38; Rv280; Rv177; Rv48; Rv374; Rv151; Rv238; Rv156; Rv92; Rv3; Rv403; Rv322; Rv243; Rv330; Rv285; Rv233; Rv219; Rv416; Rv67; Rv222; Rv149; Rv279; Rv87; Rv273; Rv266; Rv25; Rv136; Rv414; Rv13; Rv289; Rv60; Rv104; Rv5; Rv165; Rv215; Rv329; Rv240; Rv19; Rv74; Rv411; Rv167; Rv56; Rv80; Rv164; Rv59; Rv313; Rv265; Rv308; Rv220; Rv258; Rv339; Rv121; Rv419; Rv418; Rv45; Rv217; Rv134; Rv17; Rv103; Rv21; Rv22; Rv2; Rv270; Rv267; Rv174; Rv257; Rv44; Rv71; Rv7; Rv27; Rv191; Rv230; Rv128; Rv407; Rv106; Rv39; Rv255; Rv74; Rv355; Rv268; Rv58; Rv173; Rv264; Rv417; Rv401; Rv144; Rv302; Rv81; Rv163; Rv281; Rv221; Rv420; Rv175; Rv86; Rv412; Rv73; Rv269; Rv214; Rv287; Rv42; Rv143.

The polynucleotides disclosed in Table 3 may be used as probes in order to select a given clone of the BAC DNA library I-1945 for further use.

The invention also provides for a BAC-based Mycobacterium bovis strain Pasteur genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on Jun. 30, 1998 under the accession number I-2049.

A further object of the invention consists in a recombinant BAC vector which is chosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-2049. This DNA library contains approximately 1600 clones. The average insert size is estimated to be ˜80 kb.

Generally, a recombinant BAC vector of interest may be chosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-2049:

X000; X0002; X0003; X0004; X0006; X0007; X0008; X0009; X0010; X0012; X0013; X0014; X0015; X0016; X0017; X0018; X0019; X0020; X0021; X0175.

The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 4.

The polynucleotides disclosed in Table 4 may be used as probes in order to select a given clone of the BAC DNA library I-2049 for further use.

Are also part of the invention the polynucleotide inserts that are contained in the above described BAC vectors, that are useful as primers or probes.

These polynucleotides and nucleic acid fragments may be used as primers for use in amplification reactions, or as nucleic probes.

PCR is described in the U.S. Pat. No. 4,683,202. The amplified fragments may be identified by an agarose or a polyacrylamide gel electrophoresis, or by a capillary electrophoresis or alternatively by a chromatography technique (gel filtration, hydrophobic chromatography or ion exchange chromatography). The specificity of the amplification may be ensured by a molecular hybridization using, for example, one of the initial primers as nucleic probes.

Amplified nucleotide fragments are used as probes in hybridization reactions in order to detect the presence of one polynucleotide according to the present invention or in order to detect mutations in the genome of the given mycobacterium of interest, specifically a mycobacterium belonging to the Mycobacterium tuberculosis complex and more specifically Mycobacterium tuberculosis and Mycobacterium bovis BCG.

Are also part of the present invention the amplified nucleic fragments (<<amplicons>>) defined herein above.

These probes and amplicons may be radioactively or non-radioactively labeled, using for example enzymes or fluorescent compounds.

Other techniques related to nucleic acid amplification may also be used and are generally preferred to the PCR technique.

The Strand Displacement Amplification (SDA) technique (Walker et al., 1992) is an isothermal amplification technique based on the ability of a restriction enzyme to cleave one of the strands at his recognition site (which is under a hemiphosphorothioate form) and on the property of a DNA polymerase to initiate the synthesis of a new strand from the 3′OH end generated by the restriction enzyme and on the property of this DNA polymerase to displace the previously synthesized strand being localized downstream. The SDA method comprises two main steps:

a) The synthesis, in the presence of dCTP-alpha-S, of DNA molecules that are flanked by the restriction sites that may be cleaved by an appropriate enzyme.

b) The exponential amplification of these DNA molecules modified as such, by enzyme cleavage, strand displacement and copying of the displaced strands. The steps of cleavage, strand displacement and copy are repeated a sufficient number of times in order to obtain an accurate sensitivity of the assay.

The SDA technique was initially realized using the restriction endonuclease HindIII but is now generally practised with an endonuclease from Bacillus stearothermophilus (BSOBI) and a fragment of a DNA polymerase which is devoid of any 5′→3′exonuclease activity isolated from Bacillus cladotenax (exo−Bca)[=exo−minus−Bca]. Both enzymes are able to operate at 60° C. and the system is now optimized in order to allow the use of dUTP and the decontamination by UDG. When using this technique, as described by Spargo et al. in 1996, the doubling time of the target DNA is of 26 seconds and the amplification rate is of 10¹⁰ after an incubation time of 15 min at 60° C.

The SDA amplification technique is more easy to perform than PCR (a single thermostated waterbath device is necessary) and is faster than the other amplification methods.

Thus, another object of the present invention consists in using the nucleic acid fragments according to the invention (primers) in a method of DNA or RNA amplification according to the SDA technique. For performing SDA, two pairs of primers are used: a pair of external primers (B1, B2) consisting of a sequence specific for the target polynucleotide of interest and a pair of internal primers (S1, S2) consisting of a fusion oligonucleotide carrying a site that is recognized by a restriction endonuclease, for example the enzyme BSOBI.

The operating conditions to perform SDA with such primers are described in Spargo et al, 1996.

The polynucleotides of the invention and their above described fragments, especially the primers according to the invention, are useful as technical means for performing different target nucleic acid amplification methods such as:

-   -   TAS (Transcription-based Amplification System), described by         Kwoh et al. in 1989.     -   SR (Self-Sustained Sequence Replication), described by Guatelli         et al. in 1990.     -   NASBA (Nucleic acid Sequence Based Amplification), described by         Kievitis et al. in 1991.     -   TMA (Transcription Mediated Amplification).

The polynucleotides according to the invention are also useful as technical means for performing methods for amplification or modification of a nucleic acid used as a probe, such as:

-   -   LCR (Ligase Chain Reaction), described by Landegren et al. in         1988 and improved by Barany et al. in 1991 who employ a         thermostable ligase.     -   RCR (Repair Chain Reaction) described by Segev et al. in 1992.     -   CPR (Cycling Probe Reaction), described by Duck et al. in 1990.     -   Q-beta replicase reaction, described by Miele et al. in 1983 and         improved by Chu et al. in 1986, Lizardi et al. in 1988 and by         Burg et al. and Stone et al. in 1996.

When the target polynucleotide to be detected is a RNA, for example a mRNA, a reverse transcriptase enzyme will be used before the amplification reaction in order to obtain a cDNA from the RNA contained in the biological sample. The generated cDNA is subsequently used as the nucleic acid target for the primers or the probes used in an amplification process or a detection process according to the present invention.

The non-labeled polynucleotides or oligonucleotides of the invention may be directly used as probes. Nevertheless, the polynucleotides or oligonucleotides are generally labeled with a radioactive element ³²P, ³⁵S, ³H, ¹²⁵I) or by a nonisotopic molecule (for example, biotin, acetylaminofluorene, digoxigenin, 5bromodesoxyuridin, fluorescein) in order to generate probes that are useful for numerous applications.

Examples of non-radioactive labeling, of nucleic acid-fragments are described in the french patent No FR-7810975 or by Urdea et al. or Sanchez-Pescador et al., 1988.

In the latter case, other labeling techniques may be also used such as those described in the french patents FR-2 422 956 and 2 518 755. The hybridization step may be performed in different ways (Matthews et al., 1988). The more general method consists of immobilizing the nucleic acid that has been extracted from the biological sample onto a substrate (nitrocellulose, nylon, polystyrene) and then to incubate, in defined conditions, the target nucleic acid with the probe. Subsequently to the hybridization step, the excess amount of the specific probe is discarded and the hybrid molecules formed are detected by an appropriate method (radioactivity, fluorescence or enzyme activity measurement).

Advantageously, the probes according to the present invention may have structural characteristics such that they allow the signal amplification, such structural characteristics being, for example, branched DNA probes as those described by Urdea et al. in 1991 or in the European patent No EP-0 225 807 (Chiron).

In another advantageous embodiment of the probes according to the present invention, the latters may be used as <<capture probes>>, and are for this purpose immobilized on a substrate in order to capture the target nucleic acid contained in a biological sample. The captured target nucleic acid is subsequently detected with a second probe which recognizes a sequence of the target nucleic acid which is different from the sequence recognized by the capture probe.

The oligonucleotide probes according to the present invention may also be used in a detection device comprising a matrix library of probes immobilized on a substrate, the sequence of each probe of a given length being localized in a shift of one or several bases, one from the other, each probe of the matrix library thus being complementary to a distinct sequence of the target nucleic acid. Optionally, the substrate of the matrix may be a material able to act as an electron donor, the detection of the matrix positions in which an hybridization has occurred being subsequently determined by an electronic device. Such matrix libraries of probes and methods of specific detection of a target nucleic acid is described in the European patent application No EP-0 713 016 (Affymax technologies) and also in the U.S. Pat. No. 5,202,231 (Drmanac).

Since almost the whole length of a mycobacterial chromosome is covered by a BAC-based genomic DNA libraries according to the present invention (i.e. 97% of the M. tuberculosis chromosome is covered by the BAC library I-1945), these DNA libraries will play an important role in a plurality of post-genomic applications, such as in mycobacterial gene expression studies where the canonical set of BACs could be used as a matrix for hybridization studies. Probing such matrices with cDNA probes prepared from total mRNA will uncover genetic loci induced or repressed under different physiological conditions (Chuang et al., 1993; Trieselmann et al., 1992). As such, the H37Rv BAC library represents a fundamental resource for present and future genomics investigations.

The BAC vectors or the polynucleotide inserts contained therein may be directly used as probes, for example when immobilized on a substrate such as described herein before.

The BAC vectors or their polynucleotide inserts may be directly absorbed on a nitrocellulose membrane, at predetermined locations on which one or several polynucleotides to be tested are then put to hybridize therewith.

Preferably, a collection of BAC vectors that spans the whole genome of the mycobacterium under testing will be immobilized, such as, for example, the set of 68 BAC vectors of the I-1945 DNA library that is described elsewhere in the specification and shown in FIG. 3.

The immobilization and hybridization steps may be performed as described in the present Materials and Methods Section.

As another illustrative embodiment of the use of the BAC vectors of the invention as polynucleotide probes, these vectors may be useful to perform a transcriptional activity analysis of mycobacteria growing in different environmental conditions, for example under conditions in which a stress response is expected, as it is the case at an elevated temperature, for example 40° C.

In this specific embodiment of the invention, Genescreen membranes may be used to immobilize the restriction endonuclease digests (HindIII digests for the BAC DNA library I-1945) of the BAC vectors by transfer from a gel (Trieselmann et al., 1992).

Alternatively, the BAC vectors may be immobilized for dot blot experiments as follows. First, the DNA concentration of each BAC clone is determined by hybridization of blots of clone DNAs and of a BAC vector concentration standard with a BAC vector specific DNA probe. Hybridization is quantified by the Betascope 603 blot analyzer (Betagen Corp.), which collects beta particles directly from the blot with high efficiency. Then, 0.5 μg of each clone DNA is incubated in 0.25 M NaOH and 10 mM EDTA at 65° C. for 60 min to denature the DNA and degrade residual RNA contaminants. By using a manifold filtration system (21 by 21 wells), each clone DNA is blotted onto a GeneScreen Plus nylon membrane in the alkaline solution. After neutralization, the blots are baked at 85° C. for 2 h under vacuum. Positive and negative controls are added when necessary. In order to perform this procedure, it may be refer-red to the article of Chuang et al. (1993).

For RNA extractions, cells grown in a suitable volume of culture medium may, for example, be immediately mixed with an equal volume of crushed ice at −70° C. and spun at 4° C. in a 50 ml centrifugation tube. The cell pellet is then suspended in 0.6 ml of ice-cold buffer (10 mM KCl, 5 mM MgCl, 10 mM Tris; pH 7.4) and then immediately added to 0.6 ml of hot lysis buffer (0.4 M NaCl, 40 mM EDTA, 1% beta-mercaptoethanol, 1% SDS, 20 mM Tris; pH 7.4) containing 100 μl of water saturated phenol. This mixture is incubated in a boiling water bath for 40 s. The debris are removed by centrifugation. The supernatant is extracted with phenol-chloroform five times, ethanol precipitated, and dried. The dried RNA pellet is dissolved in water before use.

Then labeled total cDNA may be prepared by the following method. The reaction mixture contains 15 μg of the previously prepared total RNA, 5 μg of pd(N₆) (random hexamers from Pharmacia Inc.), 0.5 mM dATP, 0.5 mM dGTP and 0.5 mM DTTP, 5 μM dCTP, 100 μCi of [α-³²P]dCTP (3,000 Ci/mmol), 50 mM Tris-HCl (pH 8.3), 6 mM MgCl₂, 40 mM Kcl, 0.5 U of avian myeloblastosis virus reverse transcriptase (Life Science Inc.) in a total volume of 50 μl. The reaction is allowed to continue overnight at room temperature. EDTA and NaOH are then added to final concentrations of 50 mM and 0.25 M, respectively, and the mixture is incubated at 65° C. for 30 min to degrade the RNA templates. The cDNA is then ready to use after neutralization by adding Hcl and Tris buffer.

The hybridization step may be performed as described by Chuang et al. (1993) and briefly disclosed hereinafter. The DNA dot blot is hybridized to ³²P-labeled total cDNA in a solution containing 0.1% polyvinylpyrrolidone, 0.1% Ficoll 0.1% sodium Ppi, 0.1% bovine serum albumin, 0.5% SDS, 100 mM NaCl, and 0.1 mM sodium citrate, pH 7.2, at 65° C. for 2 days and then washed with a solution containing 0.1% SDS, 100 mM NaCl, and 10 mM Na-citrate, pH 7.2. The same dot blot is used for hybridization with both control and experimental cDNAs, with an alkaline probe stripping procedure (soaked twice in 0.25M NaOH-0.75 M NaCl at room temperature, 30 min each, neutralized, and completely dried at 65° C. for at least 30 min) between the two hybridizations. Quantification may be done with the Betascope 603 blot analyzer (Betagen Corp.).

As it flows from the above technical teachings, another object of the invention consists in a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:

a) bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention with a biological sample;

b) detecting the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid molecules contained within the biological sample.

The invention further deals with a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:

a) bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention that has been immobilized onto a substrate with a biological sample;

b) bringing into contact the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid contained in the biological sample with a labeled recombinant BAC vector or a polynucleotide according to the invention, provided that said polynucleotide and polynucleotide of step a) have non-overlapping sequences.

Another object of the invention consists in a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:

a) bringing into contact the nucleic acid molecules contained in the biological sample with a pair of primers according to the invention;

b) amplifying said nucleic acid molecules;

c) detecting the nucleic acid fragments that have been amplified, for example by gel electrophoresis or with a labeled polynucleotide according to the invention.

In one specific embodiment of the above detection and/or amplification methods, said methods comprise an additional step wherein before step a), the nucleic acid molecules of the biological sample have been made available to a hybridization reaction.

In another specific embodiment of the above detection methods, said methods comprise an additional step, wherein, before the detection step, the nucleic acid molecules that are not hybridized with the immobilized purified polynucleotide are removed.

Also part of the invention is a kit for detecting mycobacteria in a biological sample comprising:

a) a recombinant BAC vector or a purified polynucleotide according to the invention;

b) reagents necessary to perform a nucleic acid hybridization reaction.

The invention also pertains to a kit for detecting a mycobacteria in a biological sample comprising:

a) a recombinant BAC vector or a purified polynucleotide according to the invention that is immobilized onto a substrate;

b) reagents necessary to perform a nucleic acid hybridization reaction;

c) a purified polynucleotide according to the invention which is radioactively or non-radioactively labeled, provided that said polynucleotide and the polynucleotide of step a) have non-overlapping sequences.

Moreover, the invention provides for a kit for detecting mycobacteria in a biological sample comprising:

a) a pair of purified primers according to the invention;

b) reagents necessary to perform a nucleic acid amplification reaction;

c) optionally, a purified polynucleotide according to the invention useful as a probe.

The invention embraces also a method for detecting the presence of a genomic DNA, a cDNA or a mRNA of mycobacteria in a biological sample, comprising the steps of:

a) bringing into contact the biological sample with a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention, that are immobilized on a substrate;

b) detecting the hybrid complexes formed.

The invention also provides a kit for detecting the presence of genomic DNA, cDNA or mRNA of a mycobacterium in a biological sample, comprising:

a) a substrate on which a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention have been immobilized;

b) optionally, the reagents necessary to perform the hybridization reaction.

Additionally, the recombinant BAC vectors according to the invention and the polynucleotide inserts contained therein may be used for performing detection methods based on <<molecular combing>>. Said methods consist in methods for aligning macromolecules, especially DNA and are applied to processes for detecting, for measuring intramolecular distance, for separating and/or for assaying a macromolecule, especially DNA in a sample.

These <<molecular combing>> methods are simple methods, where the triple line S/A/B (meniscus) resulting form the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules (i.e. DNA) having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. These methods are particularly fully described in the PCT Application no PCT/FR 95/00165 files on Feb. 11, 1994 (Bensimon et al.).

When performing the <<molecular combing>> method with the recombinant BAC vectors according to the inventions or their polynucleotide inserts, the latters may be immobilized (<<anchored>>) on a suitable substrate and aligned as described in the PCT Application no PCT/FR 95/00165, the whole teachings of this PCT Application being herein incorporated by reference. Then, polynucleotides to be tested, preferably under the form of radioactively or non radioactively labeled polynucleotides, that may consist of fragments of genomic DNA, cDNA etc. are brought into contact with the previously aligned polynucleotides according to the present invention and then their hybridization position on the aligned DNA molecules is determined using any suitable means including a microscope or a suitable camera device.

Thus, the present invention is also directed to a method for the detection of the presence of a polynucleotide of mycobacterial origin in a biological sample and/or for physical mapping of a polynucleotide on a genomic DNA, said method comprising:

a) aligning at least one polynucleotide contained in a recombinant BAC vector according to the invention on the surface of a substrate;

b) bringing into contact at least one polynucleotide to be tested with the substrate on which the at least one polynucleotide of step a) has been aligned;

c) detecting the presence and/or the location of the tested polynucleotide on the at least one aligned polynucleotide of step a).

The invention finally provides for a kit for performing the above method, comprising:

a) a substrate whose surface has at least one polynucleotide contained in a recombinant BAC vector according to the invention;

b) optionally, reagents necessary for labeling DNA;

c) optionally, reagents necessary for performing a hybridization reaction.

In conclusion, it may be underlined that the alliance of such BAC-based approaches such as described in the present specification to the advances in comparative genomics by the availability of an increased number of complete genomes, and the rapid increase of well-characterized gene products in the public databases, will allow the one skilled in the art an exhaustive analysis of the mycobacterial genome.

Materials and Methods

1. DNA-preparation. Preparation of M. tuberculosis H37Rv DNA in agarose plugs was conducted as previously described (Canard et al., 1989; Philipp et al., 1996b). Plugs were stored in 0.2 M EDTA at 4° C. and washed 3 times in 0.1% Triton X-100 buffer prior to use.

2. BAC vector preparation. pBeloBAC11 was kindly provided by Dr. Shizuya, Department of Biology, California Institute of Technology (Pasadena, Calif.). The preparation followed the description of Woo et al., 1994 (Woo et al., 1994).

3. Partial digestion with HindIII. Partial digestion was carried out on plugs, each containing approximately 10 μg of high molecular weight DNA, after three one hour equilibration steps in 50 ml of HindIII 1× digestion buffer (Boehringer Mannheim, Mannheim, Germany) plus 0.1% Triton X-100. The buffer was then removed and replaced by 1 ml/plug of ice-cold HindIII enzyme buffer containing 20 units of HindIII (Boehringer). After two hours incubation on ice, the plugs were transferred to a 37° C. water bath for 30 minutes. Digestions were stopped by adding 500 μl of 50 mM EDTA (pH 8.0).

4. Size selection. The partially digested DNA was subjected to contour-clamped homogenous electric field (CHEF) electrophoresis on a 1% agarose gel using a BioRad DR III apparatus (BioRad, Hercules, Calif.) in IX TAE buffer at 13° C., with a ramp from 3 to 15 seconds at 6 V/cm for 16 hours. Agarose slices from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were excised from the gel and stored in TE at 4° C.

5. Ligation and transformation. Agarose-slices containing fractions from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were melted at 65° C. for 10 minutes and digested with Gelase (Epicentre Technologies, Madison, Wis.), using 1 unit per 100 μl gel-slice. 25-100 ng of the size-selected DNA was then ligated to 10 ng of HindIII digested, dephosphorylated pBeloBAC11 in a 1:10 molar ratio using 10 units of T4 DNA ligase (New England Biolabs, Beverly, Mass.) at 16° C. for 20 hours. Ligation mixtures were heated at 65° C. for 15 minutes, then drop-dialysed against TE using Millipore VS 0.025 mM membranes (Millipore, Bedford, Mass.). Fresh electrocompetent E. coli DH10B cells (Sheng et al., 1995) were harvested from 200 ml of a mid-log (OD₅₅₀=0.5) culture grown in SOB medium. Cells were washed three times in ice-cold water, and finally resuspended in ice-cold water to a cell density of 10¹¹ cells/ml (OD₅₅₀=150). 1 μl of the ligation-mix was used for electroporation of 30 μl of electrocompetent DH1OB E. coli using a Eurogentec Easyject Plus electroporator (Eurogentec, Seraing, Belgium), with settings of 2.5 kV, 25 μF, and 99Ω, in 2 mm wide electroporation cuvettes. After electroporation, cells were resuspended in 600 μl of SOC medium, allowed to recover for 45 minutes at 37° C. with gentle shaking, and then plated on LB agar containing 12.5 μg/ml chloramphenicol (CM), 50 μg/ml-X-gal, and 25 μg/ml IPTG. The plates were incubated overnight and recombinants (white colonies) were picked manually to 96 well plates. Each clone was inoculated 3 times (2×200 μl and 1×100 μl of 2YT/12.5 μg/ml CM per clone) and incubated overnight. One of the microtiter plates, containing 100 μl culture per well, was maintained as a master plate at −80° C. after 100 ml of 80% glycerol were added to each well, while minipreps (Sambrook et al., 1989) were prepared from the remaining two plates to check for the presence of inserts. Clones containing inserts were then designated “Rv” clones, repicked from the master plate to a second set of plates for storage of the library at −80° C.

6. Preparation of DNA for sizing, direct sequencing and comparative genomics. A modified Birnboim and Doly protocol (Birnboim et al., 1979) was used for extraction of plasmid DNA for sequencing purposes. Each Rv clone was inoculated into a 50 ml Falcon polypropylene tube containing 40 ml of 2YT medium with 12.5 μg/ml of CM and grown overnight at 37° C. with shaking. Cells were harvested by centrifugation and stored at −20° C. The frozen pellet was resuspended in 4 ml of Solution A (50 mM glucose, 10 mM EDTA, 25 mM Tris, pH 8.0) and 4 ml of freshly prepared solution B (0.2 M NaOH 0.2% SDS) was then added. The solution was gently mixed and kept at room temperature for 5 minutes before adding 4 ml of ice-cold solution C (3M Sodium Acetate, pH 4.7). Tubes were kept on ice for 15 min, and centrifuged at 10,000 rpm for 15 min. After isopropanol precipitation, the DNA pellet was dissolved in 600 μl RNase solution (15 mM Tris HCl pH 8.0, 10 μg/ml RNase A). After 30 minutes at 37° C. the DNA solution was extracted with chloroform:isoamylalcohol (24:1) and precipitated from the aqueous phase using isopropanol. The DNA pellet was then rinsed with 70% ethanol, air-dried and dissolved in 30 μl distilled water. In general, DNA prepared by this method was clean and concentrated enough to give good quality results by automatic sequencing (at least 300 bp of sequence). For a few DNA preparations, an additional polyethylene glycol (PEG) precipitation step was necessary, which was performed as follows. The 30 μl of DNA solution were diluted to 64 μl, mixed gently and precipitated using 16 μl 4M NaCl and 80 μl of 13% PEG 8000. After 30 min on ice the tubes were centrifuged at 4° C., the pellet carefully rinsed with 70% ethanol, air-dried and diluted in 20 μl of distilled water.

7. Sizing of inserts. Insert sizes were determined by pulsed-field gel electrophoresis (PFGE) after cleavage with DraI (Promega). 100-200 ng of DNA was DraI-cleaved in 20 μl total reaction volume, following the manufacturer's recommendations, then loaded onto a 1% agarose gel and migrated using a pulse of 4 s for 15 h at 6.25 V/cm at 10° C. on an LKB-Pharmacia CHEF apparatus. Mid-range and low-range PFGE markers (New England Biolabs) were used as size standards. Insert sizes were estimated after ethidium bromide staining of gels.

8. Direct sequencing. For each sequencing reaction 7 μl BAC DNA (300-500 ng), 2 μl primer (2 μM), 8 μl reaction mix of the Taq DyeDeoxy Terminator cycle sequencing kit (Applied Biosystems) and 3 μl distilled water were used.

After 26 cycles (96° C. for 30 sec; 56° C. for 15 sec; 60° C. for 4 min) in a thermocycler (MJ-research Inc., Watertown, Mass.) DNA was precipitated using 70 μl of 70% ethanol/0.5 mM MgCl₂, centrifuged, rinsed with 70% ethanol, dried and dissolved in 2 μl of formamide/EDTA buffer. SP6 and T7 samples of 32 BAC clones were loaded onto 64 lane, 6% polyacrylamide gels and electrophoresis was performed on a Model 373A automatic DNA sequencer (Applied Biosystems) for 12 to 16 hours. The sequences of oligonucleotides used as primers are shown in Table 1.

9. DOP-PCR. As an alternate procedure we used partially degenerate oligonucleotides in combination with vector-specific (SP6 or T7) primers to amplify insert ends of BAC clones, following a previously published protocol for P1 clones (Liu et al., 1995). The degenerate primers Deg2, Deg3, Deg4, Deg6 (Table 1) gave the best results for selected amplification of insert termini.

Table 1: Primers Used for PCRs and Sequencing

Vector Specific Primers for DOP PCR-First Amplification Step:

SP6-BAC1: AGT TAG CTC ACT CAT TAG GCA (SEQ ID NO. 734) T7-BAC1: GGA TGT GCT GCA AGG CGA TTA (SEQ ID NO. 735)

Vector Specific Primers (Direct Sequencing Nested Primer for Second PCR Step)

SP6 Mid: AAA CAG CTA TGA CCA TGA TTA CGC (SEQ ID NO. 736) CAA T7-Belo2: TCC TCT AGA GTC GAC CTG CAG GCA (SEQ ID NO. 737)

Degenerate Primers:

Deg2: TCT AGA NNN NNN TCC GGC (SEQ ID NO. 738) Deg3: TCT AGA NNN NNN GGG CCC (SEQ ID NO. 739) Deg4: CGT TTA AAN NNN NWA GGC CG (SEQ ID NO. 740) Deg6: GGT ACT AGT NNN NNW TCC GGC (SEQ ID NO. 741)

Primers Used for the Amplification of M. bovis DNA in Polymorphic Chromosomal Region of Rv58:

Primer 1: AGG ACC TCA TAT TCC GAA TCC C (SEQ ID NO. 742) Primer 2: GCA TCT GTT GAG TAC GCA CTT CC (SEQ ID NO. 743)

10. Screening by pooled PCR. To identify particular clones in the library which could not be detected by random end-sequencing of the 400 BAC clones, PCR-screening of DNA pools was performed. Primers were designed for regions of the chromosome where no BAC coverage was apparent using cosmid- or H37Rv whole genome shotgun sequences. Primers were designed to amplify approximately 400-500 bp. Ninety-six-well plates containing 200 μl 2YT/12.5 μg/ml CM per well were inoculated with 5 μl of −80° C. glycerol stock cultures each from the master plates and incubated overnight. The 96 clones of each plate were pooled by taking 20 μl of culture from each well and this procedure was repeated for 31 plates. Pooled cultures were centrifuged, the pellets were resuspended in sterile water, boiled for 5 minutes, centrifuged and the supernatants kept for PCRs. As an initial screening step, the 31 pools of a total of 2976 BACs, representing about two thirds of the library were tested for the presence of a specific clone using appropriate PCR primers. PCR was performed using 10 μl of supernatant, 5 μl of assay buffer (100 mM b-mercaptoethanol, 600 mM Tris HCl (pH 8.8), 20 mM MgCl₂, 170 mM (NH₄)₂SO4), 5 μl of Dimethylsulfoxide (DMSO), 5 μl of dNTPs (20 mM), 5 μl of water, 10 μl primer (2 μM), 10 μl inverse primer (2 μM) and 0.2 units of Taq DNA polymerase (Boehringer). 32 cycles of PCR (95° C. for 30 s, 55° C. for 1 min 30 s, 72° C. for 2 min) were performed after an initial denaturation at 95° C. for 1 min. An extension step at 72° C. for 5 min finished the PCR. If a pool of 96 clones yielded an appropriate PCR product (FIG. 1A), subpools were made to identify the specific clone. Subpools representative for lane A of a 96 well plate were made by pooling clones 1 to 12 from lane A into a separate tube. Subpools for lanes B to H were made in the same way. In addition, subpools of each of the 12 rows (containing 8 clones each) were made, so that for one 96 well plate, 20 subpools were obtained. PCR with these 20 subpools identified the specific clone (FIG. 1B, lower gel portion). If more than one specific clone was present among the 96 clones of one plate (FIG. 1B, upper gel portion), additional PCR reactions had to be performed with the possible candidates (data not shown).

11. Genomic comparisons. DNA from the BAC clone Rv58 was digested with the restriction endonucleases EcoR1 and PvuII, and resolved by agarose gel electrophoresis at low voltage overnight (1.5 V/cm). DNA was transferred via the method of Southern to nitrocellulose membranes (Hybond C extra, Amersham) following standard protocols (Sambrook et al., 1989), then fixed to the membranes at 80° C. for 2 hours. The blot was hybridized with ³²P labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis type strain (ATCC 19210) or M. bovis BCG Pasteur. Hybridization was performed at 37° C. overnight in 50% formamide hybridization buffer as previously described (Philipp et al., 1996b). Results were interpreted from the autoradiograms.

12. Computer analysis. Sequence data from the automated sequencer ABI373A were transferred as binary data to a Digital Alpha 200 station or Sun SparcII station and analysed using TED, a sequence analysis program from the Staden software package (Dear et al., 1991). Proof-read sequences were compared using the BLAST programs (Altschul et al., 1990) to the M. tuberculosis H37Rv sequence databases of the Sanger Centre, containing the collected cosmid sequences (TB.dbs) and whole-genome shotgun reads (TB_shotgun_all.dbs) (http://www.sanger.ac.uk/). In addition, local databases containing 1520 cosmid end-sequences and the accumulating BAC end-sequences were used to determine the exact location of end-sequenced BACs on the physical and genetic map. MycDB (Bergh et al., 1994) and public databases (EMBL, Genbank) were also used to compare new sequences, but to a lesser extent. The organization of the open reading frames (ORFs) in the polymorphic region of clone Rv58 was determined using the DIANA software established at the Sanger Centre.

EXAMPLES Example 1 Construction of a pBeloBAC11 Library of M. tuberculosis H37Rv

Partial HindIII fragments of H37Rv DNA in the size range of 25 to 180 kb were ligated into pBeloBAC11 and electroplated into strain E. coli DH10B. While cloning of fractions I (25 to 75 kb) and II (75 to 120 kb) gave approximately 4×10⁴ transformants (white colonies), cloning of fraction III (120 to 180 kb) repeatedly resulted in empty clones. Parallel cloning experiments using partial HindIII digests of human DNA resulted in stable inserts for all three fractions (data not shown), suggesting that the maximum size of large inserts in BAC clones is strongly dependent on the source of the DNA. Analysis of the clones for the presence of inserts revealed that 70% of the clones had an insert of the appropriate size while the remaining 30% of white colonies represented empty or lacZ′-mutated clones. Size determination of randomly selected, DraI cleaved BACs via PFGE showed that the insert sizes ranged for the majority of the clones between 40 kb and 100 kb with an average size of 70 kb. Clones with inserts of appropriate size were designated with “Rv” numbers, recultured and stored at −80° C. for further use.

Example 2 Direct DNA Sequence Analysis of BACs

To characterize the BAC clones, they were systematically subjected to insert termini sequencing. Two approaches, direct sequencing of BAC DNA and PCR with degenerate oligonucleotide primers (DOP), adapted to the high G+C content of mycobacterial DNA, were used. In a first screening phase, 50 BAC clones designated Rv1 to Rv50 were analysed using both methods in parallel. Except for two clones, where the sequences diverged significantly, the sequences obtained by the two methods only differed in length. Sequences obtained directly were on average about 350 bp long and for 95% of the clones both the SP6 and T7 end-sequences were obtained at the first attempt. Sequences obtained by DOP-PCR were mostly shorter than 300 bp. For 40% of the BACs we obtained only very short amplicons of 50 to 100 base pairs from one end. In two cases the sequence obtained with the DOP-PCR differed from the sequences obtained by direct sequencing, and in these cases E. coli or vector sequences were amplified (data not shown). Taking the advantages and disadvantages of both methods into account, we decided to use direct termini sequencing for the systematic determination of the SP6 and T7 end-sequences.

Example 3 Representativity of the Library

After having determined the end-sequences of 400 BACs a certain redundancy was seen. The majority of clones were represented at least 3 to 4 times. Maximum redundancy was seen in the vicinity of the unique rrn operon, as 2.5% of the clones carried identical fragments that bridge the cosmids Y50 and Y130 (FIG. 3, approximate position at 1440 kb). The majority of clones with identical inserts appeared as two variants, corresponding to both possible orientations of the HindIII fragment in pBeloBACII. This suggests that the redundancy was not the result of amplification during library construction, but due to the limited number of possible combinations of partial HindIII fragments in the given size-range of 25 to 120 kb. To detect rare BAC clones, a pooled PCR protocol was used. Primers were designed on the basis of the existing cosmid sequences and used to screen 31 pools of 96 BAC clones. When positive PCR products of the correct size were obtained, smaller subpools (of 8 or 12 clones each) of the corresponding pool were subsequently used to identify the corresponding clone (FIGS. 1A and 1B). With this approach 20 additional BACs (Rv401-Rv420) were found for the regions where no BACs were found with the initial systematic sequencing approach. The end-sequences of these BACs (Rv401-420) were determined by direct sequencing, which confirmed the predicted location of the clones on the chromosome. A 97% coverage of the genome of H37Rv with BAC clones was obtained. Only one region of ˜150 kb was apparently not represented in the BAC library as screening of all pools with several sets of specific primers did not reveal the corresponding clone. This was probably due to the fact that HindIII fragments of mycobacterial DNA larger than 110 kb are very difficult to establish in E. coli and that a HindIII fragment of ˜120 kb is present in this region of the chromosome (data not shown).

Example 4 Establishing a BAC Map

Using all end-sequence and shotgun-sequence data from the H37Rv genome sequencing project, most of the BAC clones could then be localized by sequence comparison on the integrated map of the chromosome of M. tuberculosis strain H37Rv (Philipp et al., 1996b) and an ordered physical map of the BAC-clones was established. PCR with primers from the termini sequences of selected BACs were used for chromosomal walking and confirmation of overlapping BACs (data not shown). The correct order of BACs on the map was also confirmed more recently, using 40,000 whole genome shotgun reads established at the Sanger Centre. In addition, pulsed-field gel electrophoresis of DraI digests of selected BACs was performed (FIG. 2) in order to see if the approximate fragment size and the presence or absence of DraI cleavage sites in the insert were consistent with the location of the BACs on the physical map (FIG. 3). Comparison of the sequence-based BAC-map with the physical and genetic map, established by PFGE and hybridization experiments (Philipp et al., 1996b), showed that the two maps were in good agreement. The positions of 8 genetic markers previously shown on the physical and genetic map were directly confirmed by BAC-end-sequence data (Table 2, FIG. 3). The position of 43 from 47 Y-clones (91%) shown on the physical and genetic map, which were later shotgun sequenced, was confirmed by the BAC end-sequences and shotgun sequence data. Four clones (Y63, Y180, Y251, and Y253) were located to different positions than previously thought and this was found to be due to book keeping errors or to chimeric inserts. Their present approximate location relative to the oriC is shown in FIG. 3: Y63 at 380 kb, Y63A at 2300 kb, Y180 at 2160 kb, Y251 at 100 kb, and Y253 at 2700 kb. A total of 48 BACs, covering regions of the chromosome, not represented by cosmids were then shotgun sequenced (Cole et al., 1997), and these are squared in FIG. 3. No chimeric BACs were found, which is consistent with the observations of other research groups for other BAC libraries (Cai et al., 1995; Zimmer et al., 1997). The absence of chimeric BACs was of particular importance for the correct assembly of the M. tuberculosis H37Rv sequence. The exact position of the BAC termini sequences on the chromosome will be available via the world wide web (http://www.pasteur.fr/MycDB).

TABLE 2 Identities of genetic markers previously shown on the integrated and genetic map of H37Rv. (Phlipp et al., 1996b) which showed perfect sequence homology with BAC end sequences. GenBank BAC end Description of Accession Locus sequence genetic marker Organism n° apa Rv163SP6 Secreted M. tuberculosis X80268 alanine-proline-rich dnaJ, Rv164T7 antigen M. leprae M95576 dnaK fop-A Rv136T7 DnaJ hsp M. tuberculosis M27016 polA Rv401T7 Fibronectin binding M. tuberculosis L11920 ponA Rv273T7 protein M. leprae S82044 pstC Rv103T7 DNA polymerase I M. tuberculosis Z48057 Penicillin binding recA Rv415SP6 protein M. tuberculosis X58485 wag9 Rv35SP6 Putative phosphate M. tuberculosis M69187 transport receptor Homologous recombination 35-kDa antigen

Example 5 Repetitive End-Sequences

Repetitive sequences can seriously confound mapping and sequence assembly. In the case of the BAC end-sequences, no particular problems with repetitive sequences were observed. Although nine clones with one end in an IS1081 (Collins et al., 1991) sequence were identified, it was possible to correctly locate their position on the map using the sequence of the second terminus. Moreover, these BACs were used to determine the exact locations of IS1081 sequences on the map. Five copies of this insertion sequence, which harbors a HindIII cleavage site, were mapped on the previous physical and genetic map. In contrast, BAC end-sequence data revealed an additional copy of IS1081 on the M. tuberculosis H37Rv chromosome. The additional copy was identified by six clones (Rv27, Rv118, Rv142, Rv160, Rv190, Rv371) which harbored an identical fragment linking Y50 to I364 (FIG. 3, at ˜1380 kb). This copy of IS1081 was not found by previous hybridization experiments probably because it is located near another copy of IS1081, localized on the same DraI fragment Z7 and AsnI fragment U (FIG. 3, at ˜1140 kb). Furthermore, the position of a copy of IS1081 previously shown in DraI fragment Y1 (FIG. 3, at ˜1840 kb) had to be changed to the region of Y349 (FIG. 3, at ˜3340 kb) according to the end-sequences of BAC Rv223. The positions of the four other IS1081 copies were confirmed by the sequence data and therefore remained unchanged. In total 6 copies of IS1081 were identified in the H37Rv genome in agreement with the findings of others (Collins et al., 1991).

In addition, a sequence of 1165 bp in length containing a HindIII site was found in two copies in the genome of H37Rv in different regions. The end-sequences of BAC clones Rv48 and Rv374, covering cosmid Y164, as well as Rv419 and Rv45, that cover cosmid Y92, had perfect identity with the corresponding parts of this 1165 bp sequence (FIG. 3, at ˜3480 kb and ˜900 kb). Analysis of the sequence did not reveal any homology with insertion sequences or other repetitive elements. However, as each of the two locations showed appropriate BAC coverage, chimerism of the sequenced cosmids Y164 and Y92 can be ruled out as the probable cause.

Example 6 Using BAC Clones in Comparative Genomics

The minimal overlapping set of BAC clones represents a powerful tool for comparative genomics. For example, with each BAC clone containing on average an insert of 70 kb, it should be possible to cover a 1 Mb section of the chromosome with 15 BAC clones. Restriction digests of overlapping clones can then be blotted to membranes, and probed with radiolabelled total genomic DNA from, for example, M. bovis BCG Pasteur. Restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA must be absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv. The results of such an analysis with clone Rv58 (FIG. 3, at ˜1680 kb) are shown here. This clone covers a previously described polymorphic genomic region between M. tuberculosis and M. bovis BCG strains (Philipp et al., 1996a). EcoR1 and PvuII digests from clone Rv58, fixed on nitrocellulose membranes, were hybridized with ³²P-labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis (ATCC 19120), and M. bovis BCG Pasteur. FIGS. 4A and 4B present the results of this analysis, where it is clear that several restriction fragments from clone Rv58 failed to hybridize with genomic DNA from either M. bovis or M. bovis BCG Pasteur. On the basis of the various missing restriction fragments, a restriction map of the polymorphic region was established and compared to the H37Rv sequence data. The localization of the polymorphism could therefore be estimated, and appropriate oligonucleotide primers (Table 1) were selected for the amplification and sequencing of the corresponding region in M. bovis. The alignment of M. bovis and M. tuberculosis H37Rv sequences showed that 12,732 bp were absent from the chromosomal region of the M. bovis type strain and M. bovis BCG Pasteur strain. The G+C content of the polymorphic region is 62.3 mol %, which is the same as the average genome G+C content of the M. tuberculosis genome, hence indicating that this region is not a prophage or other such insertion. Subsequent PCR studies revealed that this segment was also absent from the Danish, Russian, and Glaxo substrain's of M. bovis BCG, suggesting that this polymorphism can be used to distinguish M. bovis from M. tuberculosis. Analysis of this sequence showed that 11 putative open reading frames (ORFs) are present in M. tuberculosis, corresponding to ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library (FIG. 5). FASTA searches against the protein and nucleic acid databases revealed that the genes of this region may be involved in polysaccharide biosynthesis. Among these putative genes, the highest score was seen with ORF 6 (MTCY277.33), whose putative product shows a 51.9% identity with GDP-D-Mannose dehydratase from Pseudomonas aeruginosa (accession number U18320—EMBL Nucleotide Sequence Data Library) in a 320 amino acid overlap. The novel M. bovis sequence of the polymorphic region was deposited under accession number AJ003103 in the EMBL Nucleotide Sequence Data Library.

As it appears from the teachings of the specification, the invention is not limited in scope to one or several of the above detailed embodiments; the present invention also embraces all the alternatives that can be performed by one skilled in the same technical field, without deviating from the subject or from the scope of the instant invention.

TABLE 3 End-sequences of the polynucleotide inserts cloned in the named recombinant BAC vectors contained in the I-1945 M. tuberculosis H37Rv genomic DNA library. RvXXXSP6 corresponds to the SP6 end-sequence of the clone RvXXX. RvXXXT7 corresponds to the T7 end-sequence of the clone RvXXX. RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081 repetitive sequence (Insertion element). The character << - >> denotes an uncertain base residue. Clone Rv101 :::::::::::::Rv101SP6.seq::::::::::::: AATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTG (SEQ ID NO. 6) GCGGTACCGCGCCACCGATTCTATGCCGTGGTCGCGGAAAAATGCCTCCCGAAATCGCACGGCCGACTCCAGTTCGGC GAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGCCACGGCACCCACATGCGGCAGTTCGTCCACCTGGGCCAG CGCCCCGCCGCCGAATTCCAAACAATAGAACTGCACCCGGCCCGCATCGTGGGTAACAGCCAACGCCATGATCAGCGT CCGCAGCGCGGTTGACTTGCCCGTTTGCGGTGCACCTACGAACGCGACATTGCCTGCGGCCCCGGACAAGTCGATCGT GCGCGGCACCCGTGACTGCTCTAACGGGCGATTGAAATTCCGAT :::::::::::::Rv101T7.seq::::::::::::: CCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGG (SEQ ID NO. 7) TTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGCCGTTTCAACACCTCGCGTCGCCCTCCGACCGCGAACATTCGGGG ATGGCAGCAACCTGCTGGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTAC ACTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCCGACGCAACGGTTCCTACACCGCGATATGTTCGCCT CGCTGCCCCGGTGGACCGGT Clone Rv102 :::::::::::::Rv102SP6.seq::::::::::::: AATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGG (SEQ ID NO. 8) ATCCCAAAGTGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTC GGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCAGC ACTCAATGCCGACCAGGCCGAGGGCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACCAACATGGTGATCGCAGC AATGTTGCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCT :::::::::::::Rv102T7.seq::::::::::::: GTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGC (SEQ ID NO. 9) GGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGC TTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCG ACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGAC GACATCGTGGCGAGATTCGCCGGGTACGCCGATGAGGTGGT Clone Rv103 :::::::::::::Rv103SP6.seq::::::::::::: AATACTCAAGCTTTCGGCGGAAACGGACACATTGCGAATATTGATGACAAAATAAAAATCATTGATGGTTTGAGTCAC (SEQ ID NO. 10) CAGGCCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGA TTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTG CACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCAC :::::::::::::Rv103T7.seq::::::::::::: TGCTCCCGAAACCTGGGGGTGTGCCTGCTCTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCCGTCGAAC (SEQ ID NO. 11) CAGCCACGTGTCCATCATAGNGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTG CTGTTAGCTTCATTTGCTGCGATCAAAACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCC GCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACGACGACAATGTGACCGGGGGAGGTGCA ACCACTGGCCAGGCGTCGGCAAAGGTCGATTGCGGGGGGAAGAAGACACTCAAAGCCAGTGGGT Clone Rv104 :::::::::::::Rv104SP6.seq::::::::::::: ATACTCAAGCTTTGCCGACGAGCGGGCGATGTTGATGACGGGAAACCCCAGCGCACAACCGACGATTTTGGCGTAGCC (SEQ ID NO. 12) GGCGGACGTCTGCTCGATTCCGATCACGTCGGCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCCCC GTCGTCGAGGAGCACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATTCGGCGAAAAACCGTTCTACGTG GCGGGTGTACTGGGTGTCGAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAGATGCCGTCGGGCCG CATCGCGTCGACAACTCCGGGTGAGTGGAATAGCACTTGCCGATCACCGCGACGTTGCGCGGATGAGGCGGAACCCGA ATA :::::::::::::Rv104T7.seq::::::::::::: TCCTATGTCCCTGCCGAGCANGTGATCGAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTT (SEQ ID NO. 13) TTCCAGGAATTCGGCAAAATCGGTAAGAGCCTGAAGAATTCGGTATCGCCGGACGAAATCTGCGACGCATACGGGGGC ATATACGCTTCGGGTTTACGAGATGTCGATGGGGCCGCTGGAGGCTTCACGTCCATGGGCCACAAAGGATGTTGTCGG CGCGTACCGTTTTCTGCAGCGGGTGTGGCGCTTGGTCG Clone Rv105 :::::::::::::Rv105SP6.seq::::::::::::: ATACTCAAGCTTGATTCCGCCGAAACCGACCGTGAGCACCCCGCCAGCCACCACGCTCGGGTCGGGCGCCGGGCCCGG (SEQ ID NO. 14) GCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCTAACCATTCCAGGCG GAGCTACATCAGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCCAGGTCGAAGTCTATACCGATATGCGCATCCGCAGC CGCCACCCTGGAGAACAGAACGATGCCCTACTAATGCTTGTCTGGCGGGGCC :::::::::::::Rv105T7.seq::::::::::::: GGTACGCTTCGGTCGCAGTCTGCGAGTGATGCATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCT (SEQ ID NO. 15) TCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGAC GGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCAT TCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCAGGACCCGAATGCT CCGGGAAACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGA Clone Rv106 :::::::::::::Rv106SP6.seq::::::::::::: GGCGTCAACGGTGTCGGAACCCGCGTCAAGCAATTGGTAGGCCTGCAGTCTGTGAATCAGGCCGACGCTGTGGCCGCC (SEQ ID NO. 16) GCGGC :::::::::::::Rv106T7.seq::::::::::::: GGCTNGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCGCCACCG (SEQ ID NO. 17) CTTTGGCCCGCGCGCCCGGCGTAGGCGCATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGGCTTTCG AGCTTTGTTCGATCAACCGGCCAGCATGGTCGANGATGCATTCGAGACCATATTCGAAATTGGTTTCATCGGGGGCCC CGATCCGATGCCCCCTCCCAGTTGCGTGAGCAANCAGCGGAGTCNTCGCGGGATCGATGGCCACGGGGTGTTCAATGG CGGATGGTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAACCGATCTAGCACCACCGATCCGCGCACGTNG Clone Rv107 :::::::::::::Rv107T7D4.seq::::::::::::: CGTAATNTCGCGCACANCCANGACTTCTGGGGGGATCNGCTGACAGTGGTNGGATCCCAAATTGCGGATGATCGGGCC (SEQ ID NO. 18) GCCNACGTCGTTGTGTACCTCNTCNGTCACAACNAANCCGAANCGTATGACTCGGTCCACGCGGTGCGGCACATGGTG GACACCACACCGCCACCGCGCGGGGTGAAGGCCTATGTCACCGGTCCGGCAACACTCAATGCCGACCAGGCCGANGCC GGACACNANAGTATCNCTAACGTCACCGCGATCACGAGCATGGTGATCGNNCAATGTTNCTANTGATCTATCGCTCCG TAATTACCGCGGTTCTCGTCTTGATCATGGTCGCANCGAACTCCGGCGCAATCCGCGGATTCATCGNCTTGCTGCCCG ATCACATATTTTCAGCCTTTCACATTGCAACNAACCTGCTCGTCTCATGGNGATGCGGCGACACGGACTACCGATATC ATGCTCGCCGTTACACAATCNCGCCACGCCGCGAAGACNGGAAACGCTTCTACACAATNTTCNCGGGACGCCACTNAA CTTGGTTCNGGTTTGACATTGCCGCGCATGTNTGCCCAGCTTTGCCGGCTCCCCTTA Clone Rv108 :::::::::::::Rv108T7D4.seq::::::::::::: TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGC (SEQ ID NO. 19) CCTGCNGCTGCAGCANACCATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCC GCCGATCGACATCCCGGCCTTCNCCCTTTAACGG Clone Rv109 :::::::::::::Rv109SP6.seq::::::::::::: AACAGCTATGACCATGNTTACGCCAAGCTATTTAGGTAACACTATANAATACTCAAGCTTTTACGGTGATCGCGCATC (SEQ ID NO. 20) ACCTGGTTCATGAACTGGAAGCAGCGCANCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCCGCGGTCNGG TGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACNAACNACNCC AGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGT TTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAAGCCGCCGAACAAGT CCGCCCGATCGCATACTCCAACCGGTTGCGGTACTGCAGGTCAGCTGGCGTACCTCCTCNTCNCGCTCGGCGAAGTCT TGCTCCANCACGTCGCAGAACGGCAAGGAACACGTTCA :::::::::::::Rv109T7.seq::::::::::::: GACCGNNCCATGTTTCCACAATGTGGTGCCAGTNCGGNGGCTACGTGCCATCNANACACTGGCGCAGGCTATCGCACC (SEQ ID NO. 21) CGTTATCNGCTACGAACAAATCNCGGTATGCGTTCTTTANCATGAGTCGGCGACCGNCGATCATGGTCGACACCCACG ACNGAAATACGCAGATCGCCNTCNAGCNTGTGTGCCGCGGATTATCANGACTGACCTCCTGGCTGACCGGNNTGTNTG GTCGCGATGCCTGGCGCCCGGCCGGCGTGNTCGTGGTCGGCTCGGATAGCGAAGTCAGCTAATTCTCGTGGCAGCTCG AAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACCATGCNCATGTTACGGTCCCTCGGGTGCGGCCTGGCGGCGGC Clone Rv10 :::::::::::::Rv10SP6D2.seq::::::::::::: GGGATGGGCGGGCCCGCTAAACTCTTCGTGTTCCACTAACTCCGGGAGGGNCAATCTCGGGCCGTTATGGCTCACGTC (SEQ ID NO. 22) GCGTCGCCCTCCGACCGCGAACATTCGGAGTTGGCAGCAACCTGGTAGCACCCTGGCCGG :::::::::::::Rv10T7D4.seq::::::::::::: NCCGTCGTTGACAAGTAAATATGTCCGCAAAAGTCTCAGCGGCCGACTTTGCTCGCAGGTGGCGGTACCGCGCCACCG (SEQ ID NO. 23) AGTCGATGCCGTGGTCGCGGAAGAATGCCTCCCGAAATCGCACGGCCTTCCCNNTTTAAACGGA Clone Rv110 :::::::::::::Rv110SP6.seq::::::::::::: TTTAGGTGACACTATAGAATACTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCATTGGCCACCGGCGG (SEQ ID NO. 24) CGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGATGAGTGCTGAACCGTANTCGAAGTGGGCGGCGTCAGA CTCCACCCANCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCNATCCGGACAAGTTGGGGTGCGTTTGG GGCAATGACAGGTGGCNGCGGTGCGTTCGGGTCCGCCGGCGGAAGTGCTGCGTTGGGATCNCCCGCTGGGCATTCGGC NTTTTTGCGGCGGCCGGTGGTNGGGGGGCAACAGGTNTCCCNGTGCGGGTGGCGCTCAACGGTCNACGGCGCAAGCCG CCGTTGTTGGTACCNGGGGCGCTGGCTCCGGATCGCGTTGGCGGTCNCCGG :::::::::::::Rv110T7.seq::::::::::::: CTACACCATCGAATACGACGGCGTCGCCNACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGC (SEQ ID NO. 25) CGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATAC GGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGANAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCC GATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAANGTGATTGTTAACCTGGGCTACNGCGACCCGGC CTATGGTTATTCNACCTCNCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCANAAGTCNNCCCGGTCGTCATCGC CGAANCTCTCNTCCCGGGACCCACAGGGAATCNGCNATTTCNCCTACAAATCANCCACCTCCA Clone Rv111 :::::::::::::Rv111T7.seq::::::::::::: GCATGATCGGCCACCTTTCGGGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGC (SEQ ID NO. 26) CGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACTCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCACCAACG GCGCGAGCTCAACGACGTCAATCNCGTTGTCGCTTTCTACGGTCACCGACCCTGGTCACCGTAGTTCNCCCG Clone Rv112 :::::::::::::Rv112SP6.seq::::::::::::: GACACTATAGAATACTCAAGCTTGCCAACCGCCAGCCTGCATCCGGCGGCGANCACTGCTCCGCCGACCAGTACGAAC (SEQ ID NO. 27) CAACCTGCGGTGCCCAGGCCATTGACGATGTGCTGGTCGGCGCCCGCGAGTCCGCGCACCATCAACGCCGCGGGCACC ACCANGGCGGCCCCACCCTGCACGGCGACGATCATTCCGGCGCCGCTCACGGCGGGCGGGGCTCGAACANGCACAGCA TCAACGTNGTCACCCGGCCGTGACCGGCCCGCATCGTCACACCACCCAAGCCCATTGCCGTCCTCCTCAACNGGGCGA CCCGGCCCGCATCGTCACACGGNCTAAGGCCATTGCCGTCCTCCT :::::::::::::Rv112T7.seq::::::::::::: TCGGCGCCATCGGCACCTTCGAGGACCTGTATTTCGACGCCGTGGCCNACCTGAGGTTGGCGGTGGACNAAGTGTGCA (SEQ ID NO. 28) CCCGGTTGATTCGCTCGGCCTTGCCGGATGCCACCCNGCGCCTGGTGGTCGATCCGCNAANAGACAANTTGTGGTGGA NGCTTCTGCTGCCTGCGACACCCACNACGTGGTGGCACCGGGCAGCTTTAGCTGGCATGTCCTGACCGCGCTGGCCGA CNACTCCAGACNTTCCACNAANGGTCGCCNNCCCAATGTNCCGNANTGTCTCCGGNTCCCTTTACCNCCCAATGGGCN GNTTCCACNGGTTACGGGCCCCNTNCCGGCGGGTCTNCCTCCCAANCTACCAAATACGCCCGACNTTCCGGA Clone Rv113 :::::::::::::Rv113SP6.seq::::::::::::: ATACTCAAGCTTTTATGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCA (SEQ ID NO. 29) ACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGAACAGCCCGGCTTGAACCCTG AAAACCNGCTTTCCATATCCCGCGACGAAAGAACGCCAGTTCCGCTACTTAACCCCTCCGCGAACCGTCCATGGACAA CAGCGCGTTCTCCACCAACCGGGCCCGGGTGT :::::::::::::Rv113T7.seq::::::::::::: TCGGCTCAGGCCGCGCTGCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGT (SEQ ID NO. 30) GCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAAGCAAATCGCGGTATGCGTTCTTGAGCATGA GTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATC AGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGA TAGCGAGGTCAGCGAATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACAATAGCGCAGGT TACGGTCGCGCGGGGTGCGGCCTGGCGGCGGCC Clone Rv114 :::::::::::::RV114SP6.seq::::::::::::: CAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCGCGTCTACGCCGGCCCGGAGCATCCGCACAGCGCTCAGCA (SEQ ID NO. 31) GCCGGTTCCGTACGANCTCAAGCAGGTGGCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCC GGGCCCGCACAATCGTTCGTGTTGGAGCGGCCCATCCANACCGTTGGGCGCCGTAAGGANGCCGTGGTACGAATGCGC CTGGTGCCCGGCACCGGCAAGTTCGACCTCAACGGCCGCAGCTTGGANGACTACTTCCCAAACAAGGTGCACCAGCAG TTGATCAAGGCACCCCTGGTCACCGTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCGT CCGGTCAGGCCGGGCCTGCCCTGGGTATCGCCCGGGCATTGATTCTGGTATCCCCNGAAGAACCG :::::::::::::Rv114T7.seq::::::::::::: CGGTTGGCCACCGCTTCTGCGGTGCCGCCGCCGTCGACAATGACCGTGTCGTCCTTGCTGACCACCACGCGTCGGGCC (SEQ ID NO. 32) GAGCCCAGCACCTCCAAGCCCACCTCGCGCAGCACCATGCCGGCGTCGGGGTTGACCACCTGGCCACCCGTCACCACC GCCAGGTCCTCAAGGAAACGCCTTACGGCGGTCACCGAAGTACGGCCCCTTGACCGCGACCGCTTTCAACGTCTTGCG AATCGCGTTGACGACCAGCGTCGCCAACGCTTCGCCCTCCACGTCTTCAGCCACGATCAGTAGTGGCTTACCCGTTCC TGCAACCTTTTCCAGCAATGGCAACAGATCGGGAAGCGANCTGATCTTGTCTTGGTGCN Clone Rv115 :::::::::::::Rv115SP6.seq::::::::::::: CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTTTGGCTGGGTCGCCTTCGAATTCNGCGTGCACCGCTATGG (SEQ ID NO. 33) GTTGCANCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAA GGANATTCNCCTGTTCGATGCTGGGATTCGCCACGCCAAGGCATCTANCGATTACTCTCCNCGGGGTGGGAAAAGTGC CCAATCCCCCTCCCTCCAACTTTCCNAACAATCATTCCGGTTCCNCCNTCCGGTTGGNGGTAACCNNCCAATAAAACC CCTGCCCG :::::::::::::Rv115T7.seq::::::::::::: GCCCGCNCATGGCCAATCCCCGAAGACATCATTGGCCAGTGGCCGGGCGCTAACAGGTTCCAGCCCCCCACCANTGCC (SEQ ID NO. 34) GCTCGAACATGCGGTGCAACCCATTCGCAGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCG CCCAATAATGTCGTCCGCAACCAGATGCGCTCNAAAACCNCNCCGGCAGTCAGCGCACCCGACGCGANGTCGAAAGAC GTCNTCAGCGCGCCCACATGGGGTGCCAATCGGCACGGCAGGTATGCCGCGCGCAACCCGAGCGCGTGGTGCATGCCC ACGGTCCGCANGANGCGCANCACCCGCCAATGCCGAANCCCACGAAACATCGGGCGCATCCACCTTCAACC Clone Rv116 :::::::::::::Rv116SP6.seq::::::::::::: ATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGG (SEQ ID NO. 35) CGGTACCGCGCCACCGAGTCGATGCCGTGGTCGCGGAAGAATGCCTCCCGAATTCGCACGGCCAATTCCATTCCGGGA AGCATCCGCAATGCCAGCTGCGGTTGCCCCCTGCCGGCCACGGCACCCACTTGCGGCATTGCGTCCACCTGGGCCAGC GCCCCGCCGCCAAATTCCAAACAATAAAAATTGCACCCGGC :::::::::::::Rv116T7.seq::::::::::::: CCACCCGTGTATTTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCCG (SEQ ID NO. 36) CTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGG ATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTAC AGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTC CCTGCCCCGT Clone Rv117 :::::::::::::Rv117SP6D2.seq::::::::::::: CTGCCCATGTTTGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGA (SEQ ID NO. 37) GCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCNGACCCCTNTCNCAANAGGATNTTGTTCGCC GGACCCCNCTC :::::::::::::Rv117T7D4.seq::::::::::::: CCGACTTTCCGCGGTACCCGCTCAACTTTCTCTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACT (SEQ ID NO. 38) ACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACT ACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACC TGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCGCCTTT Clone Rv118 :::::::::::::Rv118SP6.seq::::::::::::: ATACTCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGT (SEQ ID NO. 39) CGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCATTCGGGGAAATACCTGCCCTGGCG CAGCTGGGGGATCCCAACTTCAATGGTTGCGGCACGGGTGTCAAATTCACGGTGGCGGTAGCCGTTGCCCTAATTGGA CCGCTCATCGCTGCTTTCGCGGTACCCCGCCCCGCACAGGGCTTCGGCTTCAGCCCCCATCAGGGCGGCAATAAACTT CAAGAGCACC :::::::::::::Rv118T7.seq::::::::::::: GAGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCC (SEQ ID NO. 40) CACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGG TCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTGCGCCGCGAAAGCGGCGGGTCGG GTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCAGCCATCGGGACATCATGCT CGCGTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCATTGGTGATGGAATCGGCGA ACTTGGCCACCCGCTGGGTGTTGACATCCTCGACGGTGGGCAATTGCGCCTCGGTAAGCTTTGCCGCGTAGCCTTTTC ATC Clone Rv119 :::::::::::::Rv119SP6.seq::::::::::::: ATACTCAAGCTTCACTGACAAGGGACGAATTCGTCGGCCGCCTGTTCGACTGGGTGGTGGCCGAGCTGGTCGCCACCA (SEQ ID NO. 41) CTCAGGCCGCGGTCACGGCGGTACCGGCGCGGGAGCAAACTCGCGCGGGCATGGCCAACTTCTTGCGGACCATCACCG CAGACGCCCGCTTCGGACCCCTGCTGTCCACCACACAGTTGGCCAACGCATTAATCACCCGCAAGCTTGCGGAATCCA CCGCCCTGTTCGC :::::::::::::Rv119T7.seq::::::::::::: TCCATCACCCGATGTGGCNGGAGCACTGCCATGTCGATCTCAACTACCACCTCCGGCCGTGGCGGTTGCGCGCCCCGG (SEQ ID NO. 42) GGGGTCCGCGCGAACTCGACGAGGCGGTCGGAGAAATCGCCANCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGA TGTACTTCGTTGAGGGGCTTGCCAACCACCGGATCGCGGTGGTTGCCAAAATTCACCATGCGTTGGCTGACGGTGTTG CCTCGGCAAACATGATGGCACGGGGGATGGATCTGCCGCCGGGACCGGAGGTCGGCCGCTATCTGCCTGACCCCGCTC CTACCAAGCGGCA Clone Rv11 :::::::::::::Rv11SP6.seq::::::::::::: AGCTTTGCAGTTGCTGAGTAATGTCGGCCAACGTCACCACAACCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCA (SEQ ID NO. 43) ACCCAATGGTGGCCGCGAGCGGCAGCTCGATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGA GGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTCCATCTGCCGTCGGAAGAAGTATCGAG :::::::::::::Rv11T7.seq::::::::::::: AGCTTCAGAACAGGCCTGTTGTGGGCCCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCCCGCACCC (SEQ ID NO. 44) CCGGCATCTCCCGGTCACGCAGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCCCAGTTCGCCGTCAATGATGC TGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATCGCGGTGCTCAGCAGCGTCTCGACAGCCA CCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCCGTC Clone Rv120 :::::::::::::Rv120SP6.seq::::::::::::: ATACTCAAGCTTCAGTTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTC (SEQ ID NO. 45) GCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCACCAAACCATCAACGCCTTCAAATTGCCG GCAATCTCGTTCAGCCAATCCAT :::::::::::::Rv120T7.seq::::::::::::: GCTCTACGCCGCCTACGGGTCGAACATGCATCCCGAGCAGATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAAC (SEQ ID NO. 46) CGGCTGGTTACCCGGGTGGCGGCTGACGTTCGGCGGCGAGGACATCNGCTGGGAAGGGGCGCTTGCCACCGTCGTCNA AGACCCAAATTCGAAGGTGTTCGTCGTGCTCTACGACATGACCCCGGCGGACGAGAAGAACCTTGACCGGTGGGAAGG CTCCGAGTTCGGTATCCACCAGAAGATCCGATGCCGCGTGGAGCGCATTTCCTCGGACACCACAACGGGATCCCGTCC TCG Clone Rv121 :::::::::::::Rv121SP6.seq::::::::::::: ATACTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCAGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGAC (SEQ ID NO. 47) CCGCGTAAGGCAAACCAGATGGTTCGCGGCACGGTCAACCTGCCCACACCGGCACTGGTTAAGAACTGCCCGCGTCGC GGTTTTCGCGGTTGGTGAAAAGGCCAATGCCTGCGTTTGCCGTGGGGGCGGATGTTGTCGGGAGTGACAATCTGATCA AAAGGATTCAGGGCGGTTGGCTGGAATTCAATGCCGCAATCGCGACACCGG :::::::::::::Rv121T7.seq::::::::::::: CCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGC (SEQ ID NO. 48) CGGATTCCACCACATCCCCTTGCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCA ACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATTGCGGC GAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTC CACCGCGACCTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTNATCTC Clone Rv122 :::::::::::::Rv122SP6D2.seq::::::::::::: GCAGCATGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAAGTAGCGTCGATGTGCTCACGGAATCGCCCCGGCACC (SEQ ID NO. 49) GCGATCTCGANGATCACCAGTGCCACCCCCTGCAGCGCNACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCC TGGGCCATCTGATTGGAGCTGGCGTANATGGCGGCGATGGTGACGATGGCCAGCGCCACATACATTGTGGCGGCCAGA ACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACGCAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAG CCTGCGACTAGCACGGCGGCGCCACTAGGAAGTACAAGAANGTGGCCACCACCCCATCCAGGATCGGGGTAAGGCTGA TGGTCCCGAAATCGACTCCGGCCTAATACATGACTCTCTCCTTTGCGTCATCGCCTTACTTGTGCGCGGAA Clone Rv :::::::::::::Rv123SP6D2.seq::::::::::::: GGGACACACCTCGATGCTGCCGCNATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGC (SEQ ID NO. 50) TTCCGCCGCGGGCGTGACGCATCCCGTTGACCGGCCGGANCNCTCTCTA :::::::::::::Rv123T7D4.seq::::::::::::: TGGGCGCCTCTTTCGGCCTTCCCNNTTTAAACGNAGCANGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCG (SEQ ID NO. 51) ATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAGAGGAAATTGGG GCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCC GTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGCCTTCCC Clone Rv124 :::::::::::::Rv124SP6D2.seq::::::::::::: CCGATCGGCGCCGCANCTGGTTGGTGTTNCGGATGAATCCGCAGCGAAAATGTAGCTGCGGTGGCGTGTCGTGACTCG (SEQ ID NO. 52) TNGGCGTCGACGCTCGTGGCAGCCACCGANCGGTTGGTCCAGGATCTGGATGGGCAAAGTTGTGCGGCCCGGCCGGTG ACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGACATGGAGTCGCCCCGGTTGGCGT CACCTCAAGCATTTCAATGGTTATGCGACCAGTTTTTGGGTTACGCCGTCAGACATCACGTCGGAGACTTGGATGAGC TGTGTCTGCCAGATAGCCCCGAATCGGGACGACCGTGGTCACGGTGCGTCTGACCACTCGGGTCGGGTCGCCCGCGCT ATCGGCATGGGTGCGTNATCACAGCGACACGCGCCTGCCCAAGGANGTNCGGNCGGACC :::::::::::::Rv124T7D4.seq::::::::::::: CGGGTTGCGGATCCACGCGTGCGGGTTGTCAGCAGCTACGGCACTGAACCGCGCCCACAGCTCGCCGATCCGCTTTCG (SEQ ID NO. 53) GTGGTTCTCGATCGACTCGCCGTAGGCGATGCGCAGCGCCTGCTCGAATATCGGGTACACGTAGGCCGGCCTTCCCNC TTTA Clone Rv126 :::::::::::::Rv126SP6.seq::::::::::::: CTTGATTTTGATCATCATGACGATCATCACCCTAATTTTGCTACCCGCACTGGTTATCGTGGGTACCGTCGTGCTTTC (SEQ ID NO. 54) CATGGGCGCCTCTTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGATTTGTACTGGATGGTGTTGGC GATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAAAGGAAATTGG GGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGT :::::::::::::Rv126T7.seq::::::::::::: GGGGATCCCTAGATCGACCTGCAGGCATGCAAGCTTGGCGTGTCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGT (SEQ ID NO. 55) GAGGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGG AACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCG GAGCNACTGTCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCC CAGACGACATCGTGGCGAGATTCGNCGGGTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTNGTTCTTCGCCG NCGGGCANGGTTACCGCAACTTCACCCAGACCTCCGACGACGAGGTGGTGGCGTCTCCTGGATCGTGCTC Clone Rv127 :::::::::::::Rv127SP6.seq::::::::::::: AAGGCTGCAGGTCGAAGCGGNTGGTTACGACTCCCTGTGTGTGATGGACCAGTTCTACTATCTGCGTCTACACGGCCC (SEQ ID NO. 56) TTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCCGACC CTGCTGGCAAAGATNATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGCGGGTTT GAACTGGAACACCGCCAGCTCGGCTTCGAGTCCGGCACTTCCAGTGACCGGTTCAACCGGCTCGA :::::::::::::Rv127T7.seq::::::::::::: CTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTT (SEQ ID NO. 57) CATCCTGACGCCGGAACAAATTGACGCNGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACAT CATTCGCACGGAGAACCTGCCGCTGCTACAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGT TCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGNCCAATGT TGCGACTCCGTTCGGGTTGTTCCAGANGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCN Clone Rv128 :::::::::::::Rv128SP6.seq::::::::::::: CGGTCATAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCA (SEQ ID NO. 58) TTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCACGACCCGAATGC TCCGGGAAACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGAGCATTGAGCACGCCGGCCAGCACCGCACGAGC CAGGCAATCAGCCGCCGCCGCACCGATCGCGGTGACCAGCTGAGTCTCCGGAGACAATGCGGCCGGCACGCCGGNCTC CGGCGGCACCGCTACNGCGCCCGTGG :::::::::::::Rv128T7.seq::::::::::::: GTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACNTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGC (SEQ ID NO. 59) CCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCGATACCGATTTGCGCATCCGCANCCGCNCCCTGGACGACAGAACC GTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCGATTGGCCGGCGCGGCGCTGGTC GCCAAGGACGACGCCGTTCCGGTGCTGATCGACGACGCGCTGGGGTTCACCGATCCGGAGCGACTATCAAGATGGGGG AGGTCTCTGACACCATCGGCCCCNACGGACATGTGATCGTGCCGACGTGCAGTCCCACCCCG Clone Rv129 :::::::::::::Rv129SP6.seq::::::::::::: GCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTCGAGCAACGCAATCCGTGCGGTACGGT (SEQ ID NO. 60) TCGGGTCGTACTCGATGTCCGCGACCTTGGCCTTGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTNNG CGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGCGACCGTGCAGCGGGC GCACCAGCGACTTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCG TGGGCTTGTNCTTGCGAATTGNCATGTCTAATCANGTCTTTCTCTCACGCTCTCGTCGCCGGGCTAGGCCGCATTGCC CTGCTCCTCCTCATCGCTTCGCTCTGCATCGTCCCCGGGCTAAGCCCGTGCCCCGAAA :::::::::::::Rv129T7.seq::::::::::::: GATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAA (SEQ ID NO. 61) GGCCGATGCTGCCGTTGCCGCGGGGGCGGATGTTGTCGGGAGTGACGATCTGATCGAGAGGATTCAGGGCGGCTGGCT GGAATTCGATGCCGCGATCGCGAACACCGGATCAGAATGGCCAAAGTCGGTCGCATCGCTCGGGTGCTGGGTCCGCGC GGCCTGATGCCCAACCCGAAAACCGGCACCGTCACCGCCGACTCCCCATGGCGTCCCGGATATCAAGGGCCGGCAAAT CAACTTCCCGGTTGATCAGCAAGGCAACCTGCCTCCNCCTCCGG Clone Rv130 :::::::::::::Rv130SP6.seq::::::::::::: ATACTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCAGAGTCGGGGGCATCCGCACCGGCTCGCA (SEQ ID NO. 62) TCGCATCATCCTCCCACGACGGGCCGCTCATCAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGG CCACTGCGACAATTCAAACACGGTGTCACACGGTGAATAGTGTCGAGATGGGCTCTGATCAACCGTCGCAAACCCGGT TTCGCATCAATAGCGGAATCCCACCGGGTTGCATGGAGGCTGCTGACCTTGGAAAACAAAATTTTTTCATTACAACAA AACAACCGCCNCGGAAACTTTGCA :::::::::::::Rv130T7.seq::::::::::::: CGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCG (SEQ ID NO. 63) AACCCGGATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATT ACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCG GGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCTGCAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTT TCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCCCGGTGAGGTCGTTTTTGTTGCAGTTGG GCGGAATCCGTGCTCTGGGTTACAGCCCCGAACTCGTCACGGCGGTGCGCGCCGACGGAGTTGTTATCACCGATCCGT TGGCCGTACCGCGCCTTGGGC Clone Rv132 :::::::::::::Rv132SP6.seq::::::::::::: TCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCG (SEQ ID NO. 64) TTTGGGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGGATCGCCCGGCTGGGCA TTCNGCGTGTTGGCGGCGGCCGGTGGTGGGGGGGCAACAGGTGTCGCCGGTGCGGGTGGCGCTGCAGCGGTCGACGGC GGCGAAGCGGCCGTTGTGGGTACCGGGGGCGCTGGCTCCGGATCGGCGTTGGCGGTCGCGGGCACCGCAACGGTCACC AAGCTGGCGCTGGCCATCGCCGCGATAGCCAGTGCCGCCAATCGTCCCTTGCGACGTGTCAAGTNGGGGTCCACCTGA TGCATGGCCAAAGAACCTACCGTGTTAACGGCNCAACNCAAGGACCGCGCCGGTCGCN :::::::::::::Rv132T7.seq::::::::::::: TTTCCGCGGTACCCGCTCAACTTTGTGTCNACCCTCAACGCCATTGCCGGCACCTACTACGTNCACTCCAACTACTTC (SEQ ID NO. 65) ATCCTGACGCCGGAACAAATTGACGCAGCGGGTCCGCTGAACAATTCGGTCCGTCCCACGAAAGAACCAGTTTTNCNT CTTTCNCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGTG TTTCAACCAACACTTAGAGTGTAATTGTAAACCTGGGCTAGGGGAAACCGGCTCTAGTTTTTCCACCNTCTCCGCCCC NTGTTTCGAATACTCCGTTCGGGTTGTCCCCAAA Clone Rv134 :::::::::::::Rv134SP6.seq::::::::::::: GCTTCCGGCTCGTATGTTGTGTGGAATTGTGACCGGATACCAATTTCACACAGGAAACAGCTATGACCATGATTACGC (SEQ ID NO. 66) CAAGCTAGTTAGGTGACACTATACAATACTCAAGCTTGCCGGCTGGTGGGCCGACCACTTCGATGGCACGACCCGTGA ACTGCTGCCCGGCCAATTCTTCTTGGTCGCCCGGACCGATGGACCGCGGCTGGGATTCCAGAAGGTGCCCGATCCCCC CCCTGGGAAAAACCGCGTGCACCTCTACTTCACGACCAACGAC :::::::::::::Rv134T7.seq::::::::::::: CCGATCGACTGATGCGCCGACAACCACGCCCCAACAACTGGAATGAACCGTCGTGACCATCATCAGCACGCGGTTGTA (SEQ ID NO. 67) GGCGACTTGCGACATGTTCAACCCGCCGTACTCGGACGGAATCTTCAAACCGAAACAGCCCAGCTCGGCCAGGCCTTT CACGTACTCGTCGGGGATCTGGGCACCACGCTCGAGGACGCTGCCGTCCACGGTGTCTAGGAATTCCCGCAGTTTGAC CAGAAACGCCTCGGTTCGGGCCTCCTCGGCGTCCGACGGCTTGGGAAATGGGTGTATGAGCCCTACGGGAAACCGGCC CACAAAGAGTTCTTTGGCGAAGGACGGTTTATCCCAACCACTTTCGCGAGATTCCTCGGCAAGGGCCCGCGCTTGCTC CTCGGTGACCTGAGTTTGCTGTGCCATCGCCGCCTCCTCCCTGA Clone Rv135 :::::::::::::Rv135SP6.seq::::::::::::: TGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACG (SEQ ID NO. 68) CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGC AGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGG CCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCG CGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTG GTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAGGCCGCCGAGCAAGTCCGCCCGATCGCATACTCC :::::::::::::Rv135T7.seq::::::::::::: GGGGGCGCTGCTGGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGA (SEQ ID NO. 69) GACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGAC CGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCCGATTATCAGGACTGAC CTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGT CAGCGAATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGC GCGGGGTGCGGCCTGGCGGCGGCCAGAGCACGAGTTCACCGATGCGCAGCTAGTGGCGACAGCGTCAGCCAAC Clone Rv136 :::::::::::::Rv136SP6.seq::::::::::::: TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACG (SEQ ID NO. 70) CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCCGTACAGGTCGCCTCCAACACGGCGGGGAAGCGACACCA GCCTACCGAGCTTGGAGTCCAGGACGCCAGCGGCGGCGTCGGTCTGCGTCGTGGTGCCGCCGGGGTGGCGTTGGCTGG CAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATAGACGCGGGCCGAGGCCGGTGCGATACCGTATT GCGTCAATTGGGACGCGGTTGTGCATTCGGCTAGCTCGGTTGCCACACCCGTCAGGGGTTCGACGTTGGCGGGTTCGG CGGGCCCCAGCACCGCTGTCACCATGCCCGCCAAGCCGACCTGCGGCGCCACCAACT :::::::::::::Rv136T7.seq::::::::::::: CGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTC (SEQ ID NO. 71) CTGCGCGCGCAGGCCGTCGAGCAGGTACAGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACG GCCCATCGACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCCGCGGTCGCCGTGCC ACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCCGACCACGAGTCGACGCGACATACCCGTGAC GGCGCCACGAACCCTGTCAACAAGCTGCATTCTTGCTTCCCTCATCCTCATCTCAACGCATCCATGCATGTTTGGGCG CATCCTGAATTANGTCAGACTGCAGGCGCTGGGCCGGCAGTGCTCGTGTATCAACCACAACTTCGGGCGT Clone Rv137 :::::::::::::Rv137SP6.seq::::::::::::: TTCCAACCCTAATTGGCTTTCGGCCCCATCCGTGAGGACGGGGTGCGGGTGCTCAACAACAACGTCGTCCGCGGGACA (SEQ ID NO. 72) CACCTCTATGCTGCCGCCATGGACGCGGTCCAACGCAAGCAGCTGATCGAGCTACAACCCCGCGCGGAACGCTTCCGC CGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCC AAGGCGGCGTGCCACGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAAACGACATC GTGGCGAGATTCGCCGGGTACGCCGATGAGGTGGTGTGTCTGGCGACGCCGGCGTTGTTCTTCGCCCTCGGGCAGGGT TACCGCAACTTCAC :::::::::::::Rv137T7.seq::::::::::::: CAGGCATGCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCG (SEQ ID NO. 73) GGATCCCAAAGTGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGAC TCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCA GCACTCAATGCCGACCAGGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCCGATCACNAGCATGGTGATCGC AGCAATGTTGCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACTCGGCCAA TCCGCGGATTCATCGCCTTGCTCGCCGAACACAACATTTTCACCTTTCACATTTGCACCAACCTGCTCTTCTCAT Clone Rv138 :::::::::::::Rv138SP6.seq::::::::::::: CACTACTCAAGCTCTCTCNTCATTACCACCCCTGTAATTTGGGATGGGCAAAAAGGCGAAGCACCGCTTGGCCACNAA (SEQ ID NO. 74) CGCCGGGAGGGACAATCTCGGGCGGCTATGGCTTCTCCCGGGAAGGCCCCAACGTACGGCGTTTCAACACGTCGCGTC GCCCTCCGACCGCGAACATTCGGGGATTGGCACCAACCTGNTACCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCG CGGGTAGTCCCCGCCCGGGCGGCTACAGTCTGAAACCCCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACG GTTCCTACACGGCGGATATGTTCTCCTCGCTGCGCCGGTGGACCGGTGGGTCTATCCCCTGAAACCGACATCCCN :::::::::::::Rv138T7.seq::::::::::::: CAGGCATGCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGG (SEQ ID NO. 75) CCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCC GGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCG GCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGG GCCGCGCTGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAAGAACATCAACCTCTGCAACAACGGCGACC CATTTGTTCGGACGGCAACCGGTGGCAACGCACCTAAGCTACTTGCCCGGGATGA Clone Rv139 :::::::::::::Rv139SP6.seq::::::::::::: GTTTATGCACTGGTTAGGTGTTTCCATGAGTTTCATTCTGAACATCCTTTAATCATTGCTTTGCGTTTTTTTATTAAA (SEQ ID NO. 76) TCTTGCAATTTACTGCAAAGCAACAACAAAATCGCAAAGTCATCAAAAAACCGCAAAGTTGTTTAAAATAAGAGCAAC ACGTACACAAGGAGATAAGAAGAGCACATACCTCAGTCACTTATTATCACTAGCGCCCGCCGCAGCCGTGTAACCGAG CATAGCGAGCGAACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATA TCCACCGTGGGGAAAAACTCCAGGTAGAGGTAC Clone Rv13 :::::::::::::Rv13SP6.seq::::::::::::: ATACTCAAGCTTGGGTGTAGCCGATCACCGGAAGTCNCATGATCAGCCACGTTCCGCGCCGCCCGGCATACGGTGGTG (SEQ ID NO. 77) TACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAA :::::::::::::Rv13T7.seq::::::::::::: AGCTTTATCGAAAGCGCGAACAGCTCGCGGCGGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCA (SEQ ID NO. 78) GGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGC ACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCATC GTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAATGGTTCGCCTGATCACCTACCATCGGCCA Clone Rv140 :::::::::::::Rv140SP6.seq::::::::::::: TCAACACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCTCCAGGCTNCTCCGCTCGGTGATGGCACC (SEQ ID NO. 79) CCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCCCGGCCGCCCAGTGTTCGG GCCCTCTCGCCCAGGTCGAGGTCGACACCGATTTGCGCATCCGCAGCCGCACCCTGCGACGACAGAACCGCGGCCCTA CCCACTGCTTGTCGGGCGGGGGCCAAAGAACCAGCTTGNCATCCTGCCACAATTGGCCGGCGCCCG :::::::::::::Rv140T7.seq::::::::::::: CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGAC (SEQ ID NO. 80) CTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCCAGCGCTC GGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGG GTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCT AAGCAACAC Clone Rv141 :::::::::::::Rv141SP6.seq::::::::::::: AATATTCAAGCTTTCGGCGGAAACGGACNCCTTGCGAACATTGATAACAAAATAGAAATCATTGATGGTTTGAGTCAC (SEQ ID NO. 81) CAGGCCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGA TTCCGTCNTTATCAGCCNAAATAACTGCTCTCGGGTACCACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTG CACAACATTAAATGTCTCGGTATTGTTGATTAAAAAGATACCCACCACCAGGGCAATCCAACTGAGAGCGGTTAAATT GACCGTAAAAACCTCCCGTCATCTGTTT :::::::::::::Rv141T7.seq::::::::::::: CAGGCATGCAAGCTTGCTGCATCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATA (SEQ ID NO. 82) CGGACATCCTTCCCCTGATACCCGCGGTCGAACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGAC GGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAAAACAGCTGGTCGGCCGTTA GGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAAGTGTCCTGTCTGCGGGTGCGCTGGTGTTGTCCGCGTGT GGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCCGCGAAAGTCCATTGCNGGGGGAAGAAG ACAC Clone Rv142 :::::::::::::Rv142IS1081.seq::::::::::::: GAAAGTGCCCCAAGGTGTTGGTGAAACTCGCTGGACGGTCCCCAGGATGTTGGCAGCACATTCACCGGACATGACCGG (SEQ ID NO. 83) AGCAAGACCGGACATCCTCCCATACCGTCGTCGCCGTGTACATCCGTAGCCCGTCCTGGCAGGTGCTGGGTTGAACAA AATCAGCCCAACACCTGCCACGACGAAGAAGCGGGTTGCGCTGGCATGTCTTGTCGGCTCGGCGATCGAATTCTACGA ATTCCTTATCTACGGGACCGCTGCGGCGCTGGTGTTTCCCACCGTGTTCTTCCCACACCTGGATCCCACGGTGGCCGC CGTGGCCTCCAAGGGGACATTTGCTGTGGCGTTCCTATCCCGGCCGTTCGGCGCGGCCGTCTTTGGATACTTTGGAGA CCGCCTCGGCCGCCAGAAGACCCTGGTCGCCACACTGTTGATCATGGGCCTGGCAACCGTGACTGTTGGGCTGGTTCC ACGACAGTGGCCATCGCGC :::::::::::::Rv142SP6.seq::::::::::::: ATATTCAAGCTTTGTCACACCAAGTGTTCCGACCAANCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGT (SEQ ID NO. 84) CGCCACCACGCTGGTCAGTGCGCGTTCATCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCG CAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGA CCGCTCATCGCTGCGTTCGCGGTAGCCCNCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCCAAGGCGGCGATGAACG TCGAGAGCAGCCCGCGCAGCAAATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCATAAGATG AGAAGAGGTCAGTGCGTCCTTTCCTTCG :::::::::::::Rv142T7.seq::::::::::::: CAGGCATGCAAGCTTTTTGAGCGTCTCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACG (SEQ ID NO. 85) GATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTG CCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTC GCGGTTGCGCCGCCAAAGCGGCGGGTCGGGTGCCATCATGAATGCCTCACCGCCGCCGCACTGCACGGCCAGTGCCCC GGCGATGTCAGCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCCGCGGAACAGCTCCATTCCCGGACC GCCCAACGC Clone Rv143 :::::::::::::Rv143SP6.seq::::::::::::: ATACTCAAGCTTTTGGCTGGGTCGCCTTCCAATTCAGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCNCCGCACA (SEQ ID NO. 86) CCCCACTGGCCCGGGTGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAAGGAGATTCNCCTGTTCGATGCTGGGA TTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCT CCGACGATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGA TTCTGTCCCGTTGTGTCCAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCC CGGTGAGGTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACAGCCCCGAACTCGTCACGGCGGTGCGCC GCCGAC :::::::::::::Rv143T7.seq::::::::::::: CAGGCATGCAAGCTTCAACCTATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCA (SEQ ID NO. 87) TTGGCCAGTGGCCGGGCGCTAACAGGTTCCAGCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGG CCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGTGTCGTCCGCAACCAGATGCGCT CGAAAACCGCCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAAT CGGCACGGCAGGTAGGCCGCGCGCAACCCCAACGCGTGGTGCATGCCACGGTCCGCAGGAGGCCACCACCC Clone Rv144 :::::::::::::Rv144SP6.seq::::::::::::: ATACTCAAGCTTCCCGGCCGCAGGTGACGGCGCGGCCTAGCGCCACTTGATGCCGCACCCGATCGACGGNCGTTGGTC (SEQ ID NO. 88) GGGGTTGACTGGCCGCCCGGCGAGCAGGGCGTCAACCGCGGCCCGGACGTCGGCGGCCGTCACCGGTCGGCCATTGCC CGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGGCCGTCGAAGACAAACGTGTCGGGTGTGCAGGC CGCGGAGAAGGCGCNGGCGACGTCTCGGGTTTCGTCGTAGAGATACGGGAACGTCCAGCCGTGGCGGCGGGCCTCGGC GACCATCTGATCGGGCCCGTCCTGCGGGTAGGTGACCACGTCCTTACTGGAGATACCGACCATCGGGACCCTTTGATC GGCGAGGTCCCGGCCGACCGTGGCCAATCCGGCGGCGACGTGTCGCCCGTACCGGCCAGTGGTTC :::::::::::::Rv144T7.seq::::::::::::: CAGGCATGCAAGCTTTANCANCATCAACCCCGCCCCGCACCAGCACCGACACGATGTCGATGCCATCGAGGTGAATGT (SEQ ID NO. 89) CGAACTGGCNCAAACCATCTGGCGACCGCGACCACCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCC GACGGGCCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCAGCCGGCCGTTATACTCACGCACCCCTACCGTGTCA CGCCCAAAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCGCACCCAGTGTCGTGCCCGGATCCGCCGACCAATCC CGCACCCACGTCGCCAAACCCGAAATCACCGTGATGCCGTGGTAACTGACCACCGACAGTAACGTCACTACGGCCGCC ACGCCGACGCCGAACCACCACGCACATGATGATCGGCTG Clone Rv145 :::::::::::::Rv145SP6.seq::::::::::::: ATATTCAACCTTGCACACATTGACGATACCTTGGTCACGAGACCCCAAAAGCTGGCCTCCACCGCGCGCCGGGGACCA (SEQ ID NO. 90) CGGTCATACCTTGANNCNGCTTTCGATCGTTGATGCTGCGTCTTGGTCCGCGGAAACCGCAGGCTGGCATATGCACGT GGGCGCACTGGCGATCTGCGATCCCCACCGATTCGCCCGAATACAGCTTTCAGCGGCTCCCCAAGTTGATCATCGACC GGCTGCCGGATATCCCGCACTTGCGGTGGCGGGTCACCGGCGCCCCGCTCGGACTGGACCGGCCGTGGTTCGTCGAGG ACCACGAAC :::::::::::::Rv145T7.seq::::::::::::: CAGGCATGCAAGCTTCATGCCCGCGGCATGATAGCCACATGCACGCAATCGAACTCAGCGAAACCGGCGGGCCAGGCG (SEQ ID NO. 91) TCTTACGCCACCTCACCAGCGCGCAACCTCAACCCGGCCACGGAGACCTCCTGATC Clone Rv146 :::::::::::::Rv146SP6.seq::::::::::::: ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAATTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGT (SEQ ID NO. 92) CGTGCTTTCCATGGGCGCCTCTTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGAT GGTGTTGGCGATGTCGGTGATCCTGCTCNTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAGA GGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCAT GGTGTTCGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGGCCT GGGCTTGCTGTTCGACACCCTCGTCGTGCCTCGTTCATGAAACCGTCCATTGCTGCCCTGCTGGGACCTGGTTCTGGT GGCCGCTACGGGTGCGCCCGCGCCCGGCAGTCAAATCTTCCGCCG :::::::::::::Rv146T7.seq::::::::::::: CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTC (SEQ ID NO. 93) AACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTA CAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGAC GGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAACGTGGTGCTGGCG GTCCCCATCGGCCCAGACGACATCGTGGCGAGA Clone Rv147 :::::::::::::Rv147SP6.seq::::::::::::: ATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCCCTTCCTTTTCGGCCGCA (SEQ ID NO. 94) ACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAAC CAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTT CTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCANGTGGTCCACACTGCCGAAG :::::::::::::Rv147T7.seq::::::::::::: TAGTCGCTGACCGGTGCAGGTTTCGACNATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCT (SEQ ID NO. 95) ATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGAC ACCCACGACGGAAAGACGCAGATCGCCGTCTANCNTGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGC ATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGG Clone Rv148 :::::::::::::Rv148SP6.seq::::::::::::: ATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGAACTTCTGGGGGGATCCGCTGACAGCGGCGGGA (SEQ ID NO. 96) TCCCAAAGTGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCG GTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCAGCA CTCAATGCCGACCAGGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCA ATGTTGCTAGTGATCTATCGCCCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACCTCGGCGCAATC CGCGGATTCNTCGCCTTGCTCGCCGACCACAACATTTTCAGCCTTTCAACATTTGCGACAACCTGCTCGTTCTCATGG CGATTGCNGCGAAC :::::::::::::Rv148T7.seq::::::::::::: CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTC (SEQ ID NO. 97) AACGACGACGTCGTCCGCTGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTA CAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGAC GGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCG GTCCCGATCGGCCCAGACGACATCGTGGCGAGATTCGCCGGGTACGCCGATGAAGTGGTGTTGTTTGGCGACCCGGCG TTGTT Clone Rv149 :::::::::::::Rv149SP6.seq::::::::::::: ATACTCAAGCTTTGGCATTGTGCACATTTTCCACCCGTGCTCTATTAATGCTGAGCCGCTAATTGTGACCCCAGTCGG (SEQ ID NO. 98) GAAACACGCGGAGCACCAAATTCACCGCAGCGGCCGGGGCGGTTCAACTCACCATGGATCGCTCTCGTCGTCTGGTGC TGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGCTTCTGCTGCCGCGGCTTCTTCCGGCGATGGTAACCCCCA GGTTTCGCCCACGGTCTTACGTAGCAGTGCGACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGAT GAAGGCGTACTGGGCCGACTGCGCCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAACGAGCTGCGACTC ACTCTTCCAGGCCACCCTGGCCGAAAGCTCGACATGGTCAATCCGGCCG :::::::::::::Rv149T7.seq::::::::::::: CAGGCATGCAAGCTTGCGGGCCGGAGTGGTTTCGACGGCCGCTCGCTTCTCGGCATCGGTTTGGGCTGTCACCAGCAG (SEQ ID NO. 99) TTGGTAGTTCTTCACGTACTGTTGTTCGAGCGTCGAGCCGCCGCGCGTGTCGAGGTCGCCGGACGCGTATCCCGCCAG GCCGGTCAGGGTGCCCTTCCAGTCCACGCCGCTGTGGTCGGCGAACCGCTTATCTTCAATCGAGACGATCGCCAGCTT CATCGTGTTGGCGATCTTGTCCGAGGGCACCTCGAACCGGCGCTGCGAGTACAGCCACGCGATCGTGTTGCCCTTCGC GTCGACCATCGTCGATACCGCAGGCACTTGCCCCTC Clone Rv14 :::::::::::::Rv14SP6.seq::::::::::::: ATACTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCC (SEQ ID NO. 100) GGCGGCGAAATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCG GTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCC GGCAGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATC GGCCAGGATCTGCGTGTCATCACAACGCTCGCCAAGGAGGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTC GGAATCGACTATCCGATAGTGTCCGCGCCAATGGACTTGATCGCCG :::::::::::::Rv14T7.seq::::::::::::: AGCTTCGGTGTAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGAT (SEQ ID NO. 101) CTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAG TTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGA CCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGAC GCCACCCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATTCCATTTGACGGGACGCCGACCCCGCAGCCCCAGTAC CGCCCACGACCACGCCGGCTGACCCACCACTGTACGAACACCAAGGCGACGCCGA Clone Rv15 :::::::::::::Rv150SP6.seq::::::::::::: ATACTCAAGCTTCGGTGGCTTCGCCCGCCCTGCCGGGTGGACTTCATGACAACGCGGGGGCGATTACCCCCGCTACCG (SEQ ID NO. 102) CCAGCAGCATGACGGCGGTACCTAACACCGCCCGGATGCCTCGCACGTGCCTCGATGTGCTCACGGAATCGCCCCGGC ACCGCGATCTCGAGGATCACCAGCGTTACCCCCGGCAGCGCGACACCGACAATTCCGTACACCGCCACGCCGATCCGG CCCTGGGCCAGCTGATTGGAGCTGGCG :::::::::::::Rv150T7.seq::::::::::::: CAGGCATGCAAGCTTCCACATGTACGGATCCACGAACATCCCGTTGAACTGACAGGTGCGGCCCGGCTCGATCAGGCC (SEQ ID NO. 103) GGCCACTTGTTCTACGCGGTTACCGAAGATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGC GTTGGCCGCCGCGGCGACGATCTTGGCGTCCACGGTGGTCCGGGTCTTGCCCGCTAGCACGATCCGCGAGTCGGCCGG TCACCCGGGT Clone Rv151 :::::::::::::Rv151SP6.seq::::::::::::: ATACTCAAGCTTTCCAAGTCCCAAGTGTCGATCATGGCCAAAGAGCTCGACAAAGCCGTAGAGGCGTTTCGGACCCGC (SEQ ID NO. 104) CCGCTCGATGCCGGCCCGTATACCTTCCTCGCCGCCGACGCCCTGGTGCTCAAGGTGCGCGAGGCAGGCCGCGTCGTC GGGGTGCACACCTTGATCGCCACCGGCGTCAACGCCGAGGGCTACCGAAAGATCCTGGGCATCCAGGTCACCTCCGCC GAAGACGGGGCCGGCTGGCTGGCGTTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCAGC GACGCCCACGCCGGCCTGGTGGCCGCGATCGGGGCCACCCTGCCCGCAGCGGCCTGGCAGCGCT :::::::::::::Rv151T7.seq::::::::::::: CAGGCATGCAAGCTTCACACGTAGGCGCCGTCGATAAATGACTCCGCCGCGCTTCGCACATCCTCGTAGCGATCCTTG (SEQ ID NO. 105) GCGAGCAGGTCAACCGGGCGCTGCCCGTCGAGGAGCCGGTTTTTGGCGTGCAGCCACTGGCCGACACCTCGGGGGGTA AGCGAATCCGAGAGCAGGAGGACGAGGTCACGAAGCTGCGCCAGCCGGTCGTACCGCTCAGGGCGGATGTCGCCGGTC CGCCACCCGCGTACCGCCCGATCGGACACCTGTATGACCGCGGCGACGTC Clone Rv152 :::::::::::::Rv152SP6.seq::::::::::::: CGCGGCGGCGCATTACCCCCGCTACCGTCAGCAGCTTGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGCGT (SEQ ID NO. 106) CTATGTGCACACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGTGCCCGCCCCCTG :::::::::::::Rv152T7.seq::::::::::::: GGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGCCATTTGTTCGATGCCGTTACC (SEQ ID NO. 107) GAAGATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGGCGCGACGATCTT GGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCGGCCGGTCAGCCGGGTGAACTTCGTCGAGAG CTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTATCTCGACCAGGCCCGGGCAACCTCGGGGGTGGCGCC GACGGTGAACAGGTTGCGCTGGCCACCGCGGGTAGCCGCCGGCACTATGCCGATGCCCAGGCCGCGGATCACCGGTGC GGTCAGTCGGGTCAGGATGTCGCCCGGCCCCAGGTCGAAGATCCAGCGGGCGCCGGCCGCGTGGACACNGGTGATCTC GTCCACCATCGACTTTCTGATCA Clone Rv153 :::::::::::::Rv153SP6.seq::::::::::::: TAACTCAAGGCTTGCGTTGAGGCCCCAGGCCCATCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCAATTGACC (SEQ ID NO. 108) CCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTCGCCTTGGCTGCCGGAGAGGGGCTCCGCGGGAACCGCATGCA GGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGTCGAGGATGAACTCGGCGTTGGAATTGTCCAGCCGGC CCAATTCATCGAGCGCAGATTCGTACACATGGCCGGCGGCGACATACGCTTCACCGTGGATCTGCTCCACACGGACCG CCCTGTCGGGATCCTGCTCACGGGTAAAGGAACTTACNTGGCNCTCGGTGCC :::::::::::::Rv153T7.seq::::::::::::: CCTTCTGCGCCACCCACACCGTCAACGCCCGCGAAGTCGACGTCGTCCAGGCCATCGGCGGCCTCACGGATGGATTCG (SEQ ID NO. 109) GCGCGGACGTGGTGATCGACGCCGTCGGCCGACCGGAAACCTACCAGCAGGCCTTCTACGCCCGCGATCTCGCCGGAA CCGTTGTGCTGGTGGGTGTGCCGACGCCCGACATGCGCCTGGACATGCCGCTGGTCGACTTCTTCTCTCACGGCGGTG CGCTGAAGTCGTCGTGGTACGGCGATTGCCTGCCCGAAAGCGACTTCCCCACGCTGATCGACCTTGACCTGCATGGCC GGCTGCCGCTGCAGCGGTTCGTTTCCGAACGCATCGGGCTCGAAGACGTCGAGGAGGCGTTCCACAAGATCCATGGCG GCAAGGTATTGCGTTCGGTGGTGATGTTGTGATGGCCGCCATCGAGCGCGTCATCACCCACGG Clone Rv154 :::::::::::::Rv154SP6.seq::::::::::::: ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGT (SEQ ID NO. 110) CGTGCTTTCCATGGGCGCCTCTTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGAT GGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAAA AGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTTACCGCTGCCGGCAT GGTGTTCGCCGTTACCA :::::::::::::Rv154T7.seq::::::::::::: ATTGNCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGC (SEQ ID NO. 111) TGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGA CCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTG CCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATCGTGGCCAGATT CGCCGGGTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTTGTTCTTCGCCGTCGGGCAGGGTTACCGCAACTT CACCCAGACCTCCGACGAAGAAGTGGTGGCGTTTTCTGGATCGTGCTC Clone Rv155 :::::::::::::Rv155SP6.seq::::::::::::: ATACTCAAGCTTTTCCCGTCCGTCATCGCCCAAGCGCGTGAGGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATG (SEQ ID NO. 112) GACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTG GCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTCGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAG ATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACAC CGCCAGCTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAACCGGCTCGAANAGGCGCTACAGATCCTCGAGCCA ATGGTCAAGGGTGAGCGCCAACGTTTTTCGGCGATTGGTACCCACCGA :::::::::::::Rv155T7.seq::::::::::::: CGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTC (SEQ ID NO. 113) CGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCC TGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTC GCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAAC CAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGA CTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCGCCGGGACCAGCAGGGAATCGG CGATTTCGCCTACA Clone Rv156 :::::::::::::Rv156SP6.seq::::::::::::: ATACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCAC (SEQ ID NO. 114) AAGAAGGATTCGCTGGAGCGGTGGCTGTCCAAAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCAC AACCGTGGCCATCACNTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAAACGTTGTGGGAGTTC CTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGC CCCTGGAATCCCATGACGTATCTGCGCAACGACGTGCNCAACNCGTGGCTGATGTCNGTGGTGTTGTGGGGTGGGC :::::::::::::Rv156T7.seq::::::::::::: TCGCCACCGCACCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCG (SEQ ID NO. 115) CCGACCCCGCCCGAGGTCCTGGCCGTTACCACCGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAG GTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCGATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAG TGGCGGTCACACTCCAGGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAACAGATCTTC TGTCCGACGACTACACCACCACCCAGGCCATCGCCGCCGCCCGCGATGCCAACTTCGACGCCGTACTGGCCCCGGCGC CGGCGCTCCCCGGTTGTCAAACACTTTGCCGTGTTCGTTCACGCACTGCCCAACATCGAGCCCGA Clone Rv157 :::::::::::::Rv157SP6.seq::::::::::::: ATGAAATAAGAAGAGCACATCCCTCAGTCGGTTATCATCACTAGCGCTCGCCGCACCCGTGTAACCGATCATAGCGAG (SEQ ID NO. 116) CGAACTGGCGAGGAAGCAAAGAATATCTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCCCCGCG GGAACAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTGTGACACTCACACCCTCATCAAT GATGACGAACTACACCCCGATATCCGGTCACATGACGAAGGGAAAGAGAAGGATATCATCTGTGACAAACTGCCCTCA AATTTGGCTTCCTTAA Clone Rv159 :::::::::::::Rv159SP6.seq::::::::::::: ATACTCAAGCTTGTCGAACTCCTTCTTGAATACCGGCCGGCCATCCACAGATGCCCGGAAGAACTTCCAGGTACCCAT (SEQ ID NO. 117) GGCGGCTGGATCAGGGGGCGGCACAGTTGGTCTTGTCCTGCCTCGAGTGGCGTCGTTGTCCGGCTTGGACGGGGCTCC GACGGTACCGGAGGGCAGCGACAAAACACTTATGCACTTGGGCGACCCGCCGAGACGGTGCGACACCCATCCCGACGG CACAAGCTCAGCCGCGGCCGCTCTTGTTCTTCGTCGGATCGACATTCACCCACTTCTGACCGGGCTTGGGCGAAGGAA GCAGAA :::::::::::::Rv159T7.seq::::::::::::: GGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCA (SEQ ID NO. 118) GGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGT CGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGAC CGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTC GTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGCGCGGGGTGCGGC CCTGGCGGCGGCCCA Clone Rv15 :::::::::::::Rv15SP6D2.seq::::::::::::: GACACTATATNATACTCAAGCTTCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGAN (SEQ ID NO. 119) CCCTNTCTAGA :::::::::::::Rv15T7.seq::::::::::::: CTGTAGCCACCTGTTGCCATCCCCGTCATGCCCGACTCTGCTCATCTCGGATCCGCTGACACCCCGCTAAGGCTGCTC (SEQ ID NO. 120) CTCTCGGTGCATTACCTCACCGACGGCGAACNCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCCGGCT AACTTCACCGTCAGCTTGGACGGCGGCGCTACCGTCGATGGCGCCAGCGGGGCGATGGCCGGGCCCGGCGACCGATTC GTCNTCANCCTGTCGCGTGAACTTGCCGACGTCATCGTGGTCGGTGTGGGCACCGTGCGCATTGAGGGCTACTCCGGC GTCCGGATGGGTGTCGTCAAGCGCCCGCACCGGCAGGCCCGA Clone Rv160 :::::::::::::Rv160SP6.seq::::::::::::: ATACTCAAGCTTCGCACGCTCGGCGCGCGCGGTACCGCCCAGGTCGCCCAACAGATCGTCGATGTTCGCGTCGTCCGC (SEQ ID NO. 121) CTCGCGCACGTGGTCTGTCACCAGTCAACGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACG AGCGGGCGACTGCNATGTCATGACACCGACGGCCGCCGATGGGCCCAGGGTCTGGCAAATTCGATCTGTGCGGCCAGT GCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCAC GGCACCACGGGCGCGGGCTGGCCGGTCAGATTCCAAAATTGAAAGTACGGAACCGCTGCACCACCAA :::::::::::::Rv160T7.seq::::::::::::: ATCGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCG (SEQ ID NO. 122) CGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCG ATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGG TAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGTCGAGAGCAGCCCGCGCAGCAGA TCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCGATAAGATGANAAGAAGTCATTGCGTTATTT CCT Clone Rv161 :::::::::::::Rv161SP6.seq::::::::::::: ATACTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCG (SEQ ID NO. 123) TACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTG AATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACNACGTCAATCACGTTGTCGCTTTCTACGG TCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACAACGTCTTGCA CGCGGACGCCACCCCCCGGAT :::::::::::::Rv161T7.seq::::::::::::: GCGCNAACAGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGA (SEQ ID NO. 124) CAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGC ACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCAGCGTGATCAGGCTCG GCTCGCGAGTTCGGCGAAAAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACGACGCTCGC CAAGGAGGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTCGGAATCGACTATCCGATAGTGTCCGCGCCAAT GGACTTGATCGCCGGCGGTGAGCTGGCTGCCGCNGT Clone Rv162 :::::::::::::Rv162SP6.seq::::::::::::: ATACTCAAGCTTTCTCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGAT (SEQ ID NO. 125) CCCAAAGTGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGG TCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCAGCAC TCAATGCCGACCAGGCCGAGGCCGGAAACAAAAGTATCGCTAAGGTCACCGCGATCACGAACATGGTGATCGCAGCAA TGTTGCTAGTGATCTATCGCTCCG :::::::::::::Rv162T7.seq::::::::::::: CCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGACGCAGGCCGGTTGGACTTGTCGTGCTGGACAAGGGG (SEQ ID NO. 126) TTTAGCCGCCGAAGCAGTGACGTACATCGGCGAAGAGCAGTTCGCCTGTCGACCGACGGCGCAAACCGTGAGGCTAGG GAAGCGAGGAGCACATGGCCGCCGACCCGCAATGTACACGCTGCAAGCAAACCATCGAACCCGGATGGCTATACATCA CCGCCCATCGCCGCGGTCAAGCCGGGATCGTCGATGACGGCGCAGTACTGATTCACGTGCCCGGTGAATGCCGCACCC CGGGGAGCACTTTCCGCCAAAACTAACCCGGTTGG Clone Rv163 :::::::::::::Rv163SP6.seq::::::::::::: CGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCG (SEQ ID NO. 127) TAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAANCTGAATCCTCCAACCGGGTTGTCNATC CGGACAGGTTGGGGTGCGTTTGGGGCAATNACAGGTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAGGTGCTGCNTTG GGATCCCCGGCTGGGCATTCGGCNTGTTGGCGGCGGCCGGTGGTGGGGGGGGCAACACGTGTCNCCGGTGCGGGTGGC CCT :::::::::::::Rv163T7.seq::::::::::::: CCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACG (SEQ ID NO. 128) CCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAANTTGACGCAGCGGTTCCGCTGA CCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGAT CGGTGCCGATCGTGGGGANACCCACTGGCGAACCTGGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGG CGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAAATGTTG Clone Rv164 :::::::::::::Rv164SP6.seq::::::::::::: AGCTTCCCGAGTTCGGCTTTGGATCAAGACCCCAGTCCGCGGGCGCGATCCGGCNGCTCGGTGACTACATCAAGCCAC (SEQ ID NO. 129) AAATCGACGGCTTTCGGGGTGCCGATACCGATGACGTGGCGGATGTCGAGTGTTGAGTTCTCGGCGGGGCGGATGCTC ACCTGGCGATCACCTGCCTCTCGTTGACGATCGATCGTCTATGCCGCCGTCTCTGCGGGAACAGGCCNCCAGTACATC GCCACAGACGGGATCCACCCGCATTTCGGCTACGGTTGCTCGTTTCGGTGTTCGGACTAGTCGGTCCTGGTGACGTGC CGGTGATGCGGACCGGTCCTAGCACTGACCAATGGCCAAAATGCGGGC :::::::::::::Rv164T7.seq::::::::::::: CGGGGGGCCTCTTAATAGTGTAGGAAAGAAGCTCTACATATTCAGGAGGATTCACCATGGCTCGTGCGGTCGGGATCG (SEQ ID NO. 130) ACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGACCCGGTCGTCGTCGCCAACTCCGAGGGCTCCA GGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTGAGGTGCTGGTCTGCCAGCCCGCCAAGAACCAGGCAGTGA CCAACGTCGATCGCACCGTGCGCTCGGTCAAGCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAAT ACACCGCGCCGGAGATCAGCGCCCGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTA CCGACGCGGTTATCACGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACCCGGCCAGATCGCCGG TCTCACGTGCTGCGG Clone Rv165 :::::::::::::Rv165SP6.seq::::::::::::: ATACTCAAGCTTCATAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCC (SEQ ID NO. 131) CGCACCGCCGGCATCTCCCGGTCACGCAGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCA ATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCTTCGCGTTCACTAATCGCGGTGCTCAGCAGCGTCTCG ACAGCCACCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCGTCACCTCACCCGTCCAGCGGTCC ACCACGACACGGTCGTGCACCAGCGCGCGGGCATTCACCACCCAGGCGGTCACCGCCAGGCCGATCGCCACACCCGCC ACCATCCCCGATGCAGCCAGGCCGGGAGTAAGA :::::::::::::Rv165T7.seq::::::::::::: CTGGTGCTGGACGGAGCCTAGTACAACTTCCTCTCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGAC (SEQ ID NO. 132) ATCCTGCCGCCCGAAGTACTGGAAAAGCTCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCGAGGAG TGTGCCGACAACCCATCGGTGTACGTCGTCAGTGGTGGTTAGGTGCAGCGAGTTGCGCTGTTTGGCAACGACGGCGCC AACTTCGACAAACCGCCGTCNGTACAAGATGTTGCGGCGCGGTGGGCCGAGATCNCCGATCTGTCCGGTGCGAAAATT GCTGGATTCAAGTTGTAGAACTAAAT Clone Rv166 :::::::::::::Rv166SP6.seq::::::::::::: ATACTCAAGCTTTTCCGGCGTCGTCCACCTGACCCAAAAAGCGCAGGTGCGCCGCCAAACGGCCCGCCTGGCCGCGCA (SEQ ID NO. 133) ACTGGTCGGCGTCGCCGTGGCCGACAATCAGTAGCTGGACATCCGGAAACCGCTGCACCACCTTCGGCAGCGCGTCAA GCAAAAACGGCCATTCC :::::::::::::Rv166T7.seq::::::::::::: TTTCAGATCTCATTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTACCGGATTCAAG (SEQ ID NO. 134) CCGGTCGGTCACGCCGCGGTGGTACCGGCTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCGCGAAGTGCGT TTCGCGCACCAAGATCGCGGCCTAATGGCCGGCGATGACCGCGATGACCAGCGCGATCCAGGAAAAACCGTTCCAACC AGTGCTGGGCGGCCATCCCCG Clone Rv167 :::::::::::::Rv167SP6.seq::::::::::::: ATACTCAAGCTTCCCGACCACAAGTTGAACAGCACCGATTTCGGCGAGCACTTCGTCAACTTCCAGGGTGCCCGCACC (SEQ ID NO. 135) AAGTATTTCGACAAGTATTTCCGTCGGGCCGCCGCCGCCGGCGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGAC TCCCGCGCGTACCGGCTGCCTTGGCCCGACGGGACCACGGTTTTTGAGCTGGACCGCCCGCAGGTCCTTGATTTCAAG CGCGAGGTGCTCGCCAGCCACGGTGCCCAACCGCGCGCCCTGCGCCCGCGA :::::::::::::Rv167T7.seq::::::::::::: GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTC (SEQ ID NO. 136) CCGCGTGTCGGCAGGGCACACGCTCGGGGCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGGAGAGCAGTGCTCAGTCCG CCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGCTGACGGTCGCTGAGCAGCGCTC GCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGCGAGGCATTCGTGTTCGGCCGCCTGCGGGTCGCCCCCATCGT CGACGCGATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGACTATGTGTGCG TGGGCACGGCGAGCCGGGTGCTGTGGTACACCCACCGTTGCATGACCAAGTTGACGCCTGACTGGCTGAGCACCGCGA TCCGCTCACAGGTCGGAACGTTGGTG Clone Rv169 :::::::::::::Rv169SP6.seq::::::::::::: ATACTCAAGCTTTTGGTCTAGCCGGCCGAGCCCGATACAGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGG (SEQ ID NO. 137) CGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCGTATGCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGG CAGCGCGAAACTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGG CGGTGCGTCCGGGTCGGCCGGCGGAAGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCTGCGTGTTGGCGGCGGCCGG TGGTGGGGGGGCAACAGGTGTCTCCGGTGCGGGTGGCGCTGCACC :::::::::::::Rv169T7.seq::::::::::::: GGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTAC (SEQ ID NO. 138) CCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCG GAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAG AACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTG AAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGACTCCGTTC GGGTTGTTCCCAGAGGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCGCCGGGACCCAGCACGGAAT Clone Rv16 :::::::::::::Rv16SP6.seq::::::::::::: TTCTNTCTTCCCNNATTCGTNNNTCTCNTACTACCNGGGCCNCAAAACACCTTGGCNAACGCTCAAAGGCGNTACNGG (SEQ ID NO. 139) CACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACTGAACGGGC GAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCNCTTTTTTTATGACGGG GTCGATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCNCAGACGAGAGG ACTCGATCCCATCTANGTGTGGACNAAACAGATCTTCTGTCCGACGACTACACACCACCCAGGCCATCGCCGCCGCCC GCGATGCCAACTTCNACNCCGTNCTGGCCCCGGCGGCGGCGCTCCCCGGTTGTCAAACACCTGCCGTGTTCGTTCACN CACTGCCCAACATCNAGCCCGANCNATCCNAGGTCCGTCCAACGCCTCCGCGGCTCNCCAACCTNCTCCCNCTGATCN TCCGCACCAAACACATGCCCGACTCCNTGCNCCNATTGCTTGNATCCCT :::::::::::::Rv16T7.seq::::::::::::: CCGCTATCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAG (SEQ ID NO. 140) CGGTGGCTGTCCAAGATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTC CGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAGACGTTGTGGGAGTTCCTGCCCCGCAGTGTTATC GGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCTGGAATCCCATGACG TATCTGCGCAACGACGTGCTCAACGCGTGGCTGATGTCGGTGGTGTTGTGGGGTGGGCTGATCGCGGTCTTCGGCCCG GCGCTGATCCCGTTCGTCATCATCCAGGCAGTCTTCGGCTTCAG Clone Rv170 :::::::::::::Rv170SP6.seq::::::::::::: ATACTCATGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGANCCCAGCGAAGTCGCTAGCGTGGCCGTGTTCTTGGCT (SEQ ID NO. 141) TCGGATCTATCCTCGTACATGACCGGCACCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGC CACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGA AATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCC GGGCGTGCC :::::::::::::Rv170T7.seq::::::::::::: GGCGTCAACGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTC (SEQ ID NO. 142) GTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCA ATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCGTCACCGTCGGC GTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGCCGATGGCGCG AATCTCCCATGACGAGTCGGAATCCGCGCCTCGGCG Clone Rv171 :::::::::::::Rv171SP6.seq::::::::::::: ATACTCAAGCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCGGTGATCCCACTG (SEQ ID NO. 143) TCGCCGATCGGGGCCGCACCTGGTTGGTGTTACCGATGAATCCGCACCCAAAATGTGGCTGCGGTGGCGTTTCTTGAC TCCTTGGCGTCGACTCTTGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGGGCAAAGTTGTGCGGCCCGGCCG GTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGACATGGATTCGCCCCGGTTGG CGTCACCTCAAGCATTTCAATGGTTAT :::::::::::::Rv171T7.seq::::::::::::: ATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAAATAAAGCACGAACAGGCGGGCAAAACGTCTATCTCGGAGC (SEQ ID NO. 144) CGGAAGGGCAATCAGCCGACCGTCGACGAACGACACCGGCGATAACCACTTAGGCGTTGAACGGCCGGCCCAAACATT ACGCCTCCGTTGATAAGGCTTTCGGTCTCTTCCCCGGTCATCCCAAGCACCTTGCGGCAAATTTGAACGCTTTCCTGT CCGGGCACCGGCCCCGGGCTTTGGGGTCCNTCCGA Clone Rv172 :::::::::::::Rv172SP6.seq::::::::::::: ATACTCAAGCTTCAATCGCGCCGCCACAATCCAAATATGCGTCTAGCGTCTCGATGAGCGTCGGTCCGGCATCGGCTA (SEQ ID NO. 145) GGGGCCGCATCACGTCGGTATGCAGGGCCACGATCGCCCAAGGCGTCGCCCATCAAGGGCGCGTTCGGGCAAAAATTC CCCTATCCAGCACGGGCCGCGGCGCTCCGCNCCAGCCGGCGACGGCGTTCATCCCGGAGATCGCCTCGCTAGCGCTGC GGTGCGCCGCGGTCAGCATGGGCGCCGTGGGGCCGATGACCACCGGGGCGT :::::::::::::Rv172T7.seq::::::::::::: TTCGGCGGGTCTGTAGATTGCGGTCGGCCACCCCACAGGCACTCATGAACCGCAGCCCACGATCGATCTCGGTGG (SEQ ID NO. 146) Clone Rv173 :::::::::::::Rv173SP6.seq::::::::::::: GCGCACCATCGCCAGTAGGTGCCCGTGGTCGGGCGCGTCGAGCCACCCGAGCGGAAACGCGAGTCCGAACAGCAACAG (SEQ ID NO. 147) CAGGACGGGCGCAACCAGGGCGGTGACCATGCCCCCGGCGCTGAACATCAACCACAGGAAGGGCTCCGCCGAGCGTCC GCGCGACC :::::::::::::Rv173T7.seq::::::::::::: CATCGTCGAACTTCGGTCCGGGTTGNTAGNACCGCAGCACCAAACGCACCCACCGACCCCCACGCTTCACCCCAACCC (SEQ ID NO. 148) TTTAGTTCATTGGCGTGAACAGCAGCGTAGCCGGTTGCCCCGATATATGTGGAAAAATCGTTCGGACGTACAAAAAAA GTTCCTGACGCTGGCGTCAACTCGAAACTGCCTCGGAAGTCAATGATGATCCATCAGTCAATATTAAAGTCG Clone Rv174 :::::::::::::Rv174SP6.seq::::::::::::: ATACTCAAGCTTGTCTGCTGCCTCAGCGTATGCATCCAACAGCGCATCGCGATCAACGATCAGGCGCGCCGATTTCGG (SEQ ID NO. 149) GCCGCGGGCAGTGGCACTGGCCAGATGGCCGTTTTTTTCGAGAAACTTCAACGCCTGAGCGCTGCTTCCCATCGAGAG ACCGGTGGCCTCTACAACCGATGCGACAGTTGGACCGGCGATGTTCGCCAGCAGCGCTTCACATACGGCAAGTNTGGC GCGG :::::::::::::Rv174T7.seq::::::::::::: TTGTCCAGGCGGGGAATCGGGCAGGGAGACGACACCTTCGTTCGGTTCGATCGTCGCGAACGGGTAGTTGGCCGCGAC (SEQ ID NO. 150) CACGTTGTTTCGGGTCAGCGCGTTGAAAAGTGTCGACTTGCCGACGTTGGGCAGGCCCACGATCCCCAGGCTCAAGCT CACAGA Clone Rv175 :::::::::::::Rv175SP6.seq::::::::::::: ATACTCATGCTTGGCGCCTGGGTGGCAGCCCACCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTG (SEQ ID NO. 151) GTGGTCAGCATCGTGGCCGGTCTGCTGTTGTATGCCAACTTCCCGCCGCGCAACTGCTGGTGGGCGGCGGTGGTTGCG CTCGCATTGCTGGCCTGGGTGCTGACCCNCCGCNCNACAACACCGGTGGGTGGGCTGGGCTACGGCCTGCTATTCGGC CTGGTGTTCTACGTCTCGTTGTTGCCGTGGATCGGCGAGCTGGTGGGCCCCGGGCCCTGGTTGGCACTGGCGACGACG TNCGCGCTGTTCCCCGGCATCTTCGGTCTGTTCGCCGTCGTGGTACCCTGTTGCCGGGTTGGCCC :::::::::::::Rv175T7.seq::::::::::::: CGCCAATTCACGATATCGTTAACCGATATCCCGAGCCGATAGCTGGCGGGCTCGGGTGGTGGCCAGCGGCGCTGCGAC (SEQ ID NO. 152) GAAAGGTGTGACCGTCATGAAACAGACACCACCGGCGGCCGTCGGCCGTCGTCACCTGCTCGAGATCTCAGCATCCGC AGCCGGTGTGATCGCGCTTTCGGCGTGTAGTGGGTCGCCGCCCGACCCCGGCAAAGGCCGGCCCGACACAACCCCGGA ACAGGAAGTCCCGGTCACCGCGCCCGAAGNACTTGATGCGCGAACNCGGAGTGCTCCAAACGCATCCTGCTGAT Clone Rv176 :::::::::::::Rv176SP6.seq::::::::::::: ATACTCAAGCTTGGGCACTGACTTCGGTACCCCCTCCGCCTTTGGCCAGCAGCAGCCACAGCGCGGTTCGCGGACCGA (SEQ ID NO. 153) ACGTGGACATCAATAGCCCGGAATCGGTGTGTGCAAGTTGGTAAACGGTGTTGATCCCAAGCTTTGCCAGCCTTTTCG TAGTCTTGGGCCCCACACCCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCT GATAAATGCCAGCTGGTTTCGCGAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAG ACAGCCCGGTTTGCGACAAAATGACTTTTCGGATCTCTTCGGCGACTTCGATGGGGTCGTCGGGA :::::::::::::Rv176T7.seq::::::::::::: AAAGTCCTGTGCCGGTTCGCTAAACACCCGGCGGACACTCAGACGGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGC (SEQ ID NO. 154) AAAGCGCACTTCTCCGGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAACGTTGGTACA CAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCGCTGCCACCAGACGATGGAGCCACTCGCC GCGGAACTGAACGTGACCATACACA Clone Rv177 :::::::::::::Rv177SP6.seq::::::::::::: ATACTCAAGCTTGGGTTCCACGCCCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGG (SEQ ID NO. 155) AATCGGCCGTGACGGAATTGGCGCACCGAACACCCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGG CCACCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCA TCACCGCTGCCGCCCGGCACGTCCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAAC TGGCCTAGAACCGGCGGGCACACCGCNCCTGGGCGGGGCGAATTCTTGACCGCNCCGGCC :::::::::::::Rv177T7.seq::::::::::::: CGCGGTTGGCGTAGTTGGACGGGTCGCCCTCCGAGGCCAATGATGACGATGACCACGCCGATCACGATGGCCACCGAG (SEQ ID NO. 156) AGGGACAACAACAGAAAGCTGACGAATCCCTCCTTGGCGGCCGGGGCTTTGTGGTCGCCGGTCGCGATGGGCGCGAAT TTACGGCCCGCTCCCCCAGGCCGCCGCGAAGCAGGGTCCCCAGCCAGTTGGCGTAGGCGGAATTAACGATCAGCGCCA CCGCGATAACCTGCCATGCCTCGGGCATATCGATGTGCGGCCAGAACAGGCCGAAC Clone Rv178 :::::::::::::Rv178SP6.seq::::::::::::: CCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACG (SEQ ID NO. 157) ACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTG CGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACG ATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCGCTGGCGGGCTGATGACCGCCCTGACCCCTCAAT TCGGGTCCAAGACCATCANCCTCTGCAACAACGGCGACCCGATTTGTTCNGACGGCAACCGGTGGCGAGCGCACCTAG GCTACGTGCCCGGGATGACCAACCAGGCGGCGCGTTTCGTCGCGAGCAGGATCTAACCGCGAGCCGCCCATAGATTCC CG :::::::::::::Rv178T7.seq::::::::::::: TAANACCCGTGTAATTTGGGATGGGCAAAAAGGCCAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGC (SEQ ID NO. 158) GGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCNCCCTCCGACCGCGAACATTCGG GGATGGCAGCAACCTGGTAGCNCCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCT ACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCC TCGCTGCGCCGGTGGACCGGTGGGTCTATCCCGGAGACCGACNTCCCGATCGAAGCGACCGTCTCCTCGATGGACGCC GGCGGCGTCACCCTGGGTTTGCTCACCGCCTGGCGTGGCCCCAA Clone Rv179 :::::::::::::Rv179SP6.seq::::::::::::: GTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGATTCGATGCCGTGGTCGCGGAA (SEQ ID NO. 159) GAATGCCTCCCGAAATCGCACGGCCGACTCCAGTTCGGCGAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGC CACGGCACCCACATGCGGCAGTTCGTCCACCTGGGCCAGCGCCCCGCCGCCGAAGTCCAAACAATAGAACTGCACCCG GCCCGCATCGTGGGTAGCAGCCAACGCCATGATCAGCGTCCGCAGCGCGGTTGACTTGCCCGTTTGCGGTGCACCTAC GACCGCGACATTGCCTGCGGCCCCGGACAAGTCGATCGTCAGCGGCACCCN :::::::::::::Rv179T7.seq::::::::::::: CGTGGCCACGAACGCCGGGAGGGACANTCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCA (SEQ ID NO. 160) ACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGCAGCTACCTGGCCGGGCGATGATCTG CAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCATCATC CGACGCAACGGTTCCTACACGGCGATATGTTCNCCTCGCTGCGCCGGTGGACCGGTGGGTCTATCCC Clone Rv17 :::::::::::::Rv17SP6.seq::::::::::::: ATACTCAAGCTTTGCGGCGGGCGCCGAAATGTGAACGCACCAAACCCGCCCGCTGCGGGTCGGCGGGCCACTCGACCT (SEQ ID NO. 161) CGAATTTCGCCGCCGTGACCATCCAGCCCGACGGCAGTTGGGCACCCGGCCCCCCGGTCGCGGCATAACTGTTGGCGT CGCCGTCATAAAGCTCGAACAGCACCGAAACCGACTCCACCACCGGCCGGTGCGCCTCAAAATCCACGCCGATCTCCA CATACCGGGAAAACGTCGGTGTCCCATCGGGTTTCGGCTTGCCCGCCAGCTGCACACCACCGGTGGCCTCGGCCACCT TCGCGGCCTGAGCGCAGCTACNCATCCTGACGATCATCACCCCGCCCCCGGCTCACGCTTGGCCTCCGTCACCGCACG CATCGCCCGGTTGCGCGCACCGCGACGCCCGTACAGCCGCGCGCAC :::::::::::::Rv17T7.seq::::::::::::: AGCTTGCCGGGACTGCGGAACAGAAGCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGCCTTTTTACTAA (SEQ ID NO. 162) CCGAACCCGATGTGGGCTCCGATCCGGCGCGCATGGCATCGACGGCGACGCCGATCGATGACGGCCAGGCTTACGAGC TTGAGGGTGTGAAGTTGTGGACCACCAACGGTGTGGTAGCGGACCTGCTAGTGGTTATGGCGCGGGTACCGCGCAGTG AAGGGCACCGAGGGGGAATCAGCGCCTTTGTCGTCGAGGCTGATTCGCCCGGGATCACCGTGGAGCGGCGCAACAAGT TCATGGGACTGCGTGGCATCNAAAACGGCGTGACCCGGCTTCATCGCGTCNGGGTGCCCAAAGACAACTTGATCGGCA Clone Rv180 :::::::::::::Rv180SP6.seq::::::::::::: CTCAAGCTTGGCGATGCGGGCTGGCCAAAACTGGCCGGGCGGGGGTTGGCTTGTTCAATCAAGGGTGGGTTGCCG (SEQ ID NO. 163) :::::::::::::Rv180T7.seq::::::::::::: CCGAAGGCCCGTTCCCGGGCGTTCAGCAAGCGATCGTCGGTTGGCCCACTGCGGGTCGAATCTTGCGGCCGCGCCGGT (SEQ ID NO. 164) CGTGGAACGCCCAGGTCACCCGGCGGCGTACC Clone Rv181 :::::::::::::Rv181SP6.seq::::::::::::: ATACTCAAGCTTTTTTCTGCTCATGAAGGTTAGATGCCTGCTGCTTAAGTAATTCCTCTTTATCTGTAAAGGCTTTTT (SEQ ID NO. 165) GAAGTGCATCACCTGACCGGGCAAATAGTTCACCGGGGTGAGAAAAAAGAGCAACAACTGATTTAGGCAATTTGGCGG TGTTGATACAGCGGGTAATAATCTTACGTGAAATATTTTCCGCATCAGCCAGCGCAGAAATATTTCCAGCAAATTCAT TCTGCAATCGGCTTGCATAACGCTGACCACGTTCATAAGCACTTGTTGGGCGATAATCGTTACCCAATCTGGATAATG CAGCCATCTGCTCATCATCCAGCTCGCCAACCAGAACACGATAATCACTTTCGGTAAGTGCAGCAGCTTTACGACGGC GACTCCCATCGGCAATTTCTATGACACCAGATACTCTTCGACCGAACGCCGGTGTCTGTTGACCA Clone Rv182 :::::::::::::Rv182SP6.seq::::::::::::: CTCAAGCTTGGTGCCGACATGGCCGGGCTGGAGCCCGCGTATGGCAAGGTTCCGCTCAATGTGGTTGTGATGCAGCAG (SEQ ID NO. 166) GACTACGTTCGCCTCAATCAGCTCAAACGTCACCCCCGTGGCGTGCTGCGCAGCATGAAGGTCGGCGCCCGCACGATG TGGGCGAAGGCAACAGGTAAAAACCTGGTCGGCATGGGTCGAGCCCTCATTGGGCCGTTGCGGATCGGGTTGCACCGC GCCGGAGTGCCGGTCGAACTCAACACCGCCTTCACCGATCTTTTCGTCAAAAATGGCGTCGTGTCCGGGGTATAC :::::::::::::Rv182T7.seq::::::::::::: CCGAAGCGTGGGAAATCCTGACCGAATACCGCGACGTGCTGGACACTTTGGCCGGCGAGCTGCTGGAAAAGGAGACCC (SEQ ID NO. 167) TGCACCGACCCGAGCTGGAAAGCATCTTCGCTGACGTCTAAAAGCGGCCGCGGCTCACCATGTTCGACGACTTCGGTG GCCGGATCCCGTCGGACAAACCGCCCATCAAGACACCCGGGGGAGATCGCGATCGAAACGCGGCGAAACTTGGGCC :::::::::::::Rv183SP6.seq::::::::::::: CGACTCGACAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTT (SEQ ID NO. 168) GGTCCGGTAGCGCTCGTCCGGGTATGCTGCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAAAACGCCACCGGTC CAGCGCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAACAGGGGACATGCTTAGCGGTAGGGCGCGCGCCA AGGCGGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCG CCCGGTCATTGATCATCTTGCGCACCGCGGATAATCCGGGATTGCCAGCCCATTCGACTACCGCATGCGAGTCATCGG CTGACCGCAGCGGTCCGATTACCCGAGCGCCCCGANTACATCTCCTCCAATATCAATGGGCGCAA Clone Rv183 :::::::::::::Rv183T7.seq::::::::::::: GCGGTNTAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCT (SEQ ID NO. 169) GCTGCTGAGTGCGTGCCAGACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAAGTT GGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACGACTCCAAGCTGGCGCCGTCTCGTCCGCAGGT CGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGGATCGA CTACGAATTGATCACCCAGAACCATCGGGCGTATTACTGCCTGAAGTACCTGGTGCGGGTCGGATACTGCTATCCGGC GGTGACGACCCCCGGCAAGCCGCCATCCGTGCTGCTGT Clone Rv184 :::::::::::::Rv184SP6.seq::::::::::::: CTCAAGCTTGGGCGTGACGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGA (SEQ ID NO. 170) CGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCA CTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGAC CCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACT GGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCC :::::::::::::Rv184T7.seq::::::::::::: CGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCG (SEQ ID NO. 171) TAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATC CGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTG GGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGGTGGGGGGGCAACANGTGTCGCCGGTGCGGGTGGC GCTGCA Clone Rv185 :::::::::::::Rv185SP6.seq::::::::::::: NCTTGATATTGGCGTCAACGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGC (SEQ ID NO. 172) CGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCT GAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCGT CACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGC CGATGGCGCGAAACTCCCCATGACGAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCC GCCATTCGATCAAGTCAGCAATGGTGATCAGCGCCAGACCGTGCTCNTCGGCG :::::::::::::Rv185T7.seq::::::::::::: CATAAGGGCCGGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCG (SEQ ID NO. 173) CCACCGCTTTGGCCCGCGCGCCCGGCGTAGGCGCATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGG CTTTCGAGCTTTGTTCGATCAACCGGCCAGCATGGTCGAGGATGCATTCGAGACCATATTCGAAATTGGTTTCATCGG GGGCCCCGATCCGATGCCCCCTCCCAGTTGCGTGAGCAAGCAGCGGAGTCGTCGCGGGATCCATGGCCACGGGGTGTT CAATGGCGGATGGTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAGCCGATCTAGCACCACCGATCCGCGCACGTGGA CCGAAACCGCCGAGTAGATGTCGAAAGCGT Clone Rv186 :::::::::::::Rv186SP6.seq::::::::::::: CGTCCTTTTCCCCAAGATAGAAAGGCAGGAGAGTGTCTTCTGCATGAATATGAAGATCTGGTACCCATCCGTGATACA (SEQ ID NO. 174) TTGAGGCTGTTCCCTGGGGGTCGTTACCTTCCACNAGCAAAACACGTAGCCCCTTCAGAGCCNNATCCTGAGCAANAT GAACAGAAACTGAGGTTTTGTAAACGCCACCTTTATGGGCAGCAACCCCGATCACCGGTGGAAATACGTCTTCAGCAC GTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTCAATTGTATAACCAACACGTTGCTCAACCCGTC CTCGAATTTCCATATCCGGGTGCG Clone Rv187 :::::::::::::Rv187SP6.seq::::::::::::: CTCAAGCTTCATGTCCGTACGGCTCGGGTACGCTTCCGTCGCAGTGTGCGAGTGATAAATGACGACCGGGACCTCGTC (SEQ ID NO. 175) GGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATT GGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTC GCGACACGCATGGGCCACCATCGCATTCAC :::::::::::::Rv187T7.seq::::::::::::: NCGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACC (SEQ ID NO. 176) GCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCT CTTTCGAGGTCGAGGTCGATACCGATTTGCGCATCCGCAGCCGCACCCTGGACGACAGAACCGTGCCCTACGANTGCT TGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCGATTGGCCGGCGCGGCGCTGGTCTCCAAAGAAGACGCCC TTCCGGTGCTGAT Clone Rv188 :::::::::::::Rv188SP6.seq::::::::::::: CGCCACGTTCATGGGCAACAACCCCGATCACCGGTGGAAATACGTCTTCAGCACGTCGCAATCGCGTACCAAACACAT (SEQ ID NO. 177) CACGCATATGATTAATTCGTCCAATTGTATAACCAACACGTTGCTCAACCCGTCCTCGAATTTCCATATCCGGGTGCG GTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAAACCCCAACTAAATCCGCTGCTTCNCCTATTCTCC AGCGCCGGG Clone Rv189 :::::::::::::Rv18SP6.seq::::::::::::: ATACTCAAGCTTCAACCGATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCATTG (SEQ ID NO. 178) GCCAGTGGCCGGGCGCTAACAGGTTCCAGCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGGCCG GCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGTGTCGTCCGCAACCAGATGCGCTCGA AAACCGCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAATCGGC ACGGCAGGTAGGCCGCGCGCAACCCGAACGCGTGGTGCATGCCCACGGTCCGCAGGAGGCGCAGCACCCGCCAATGCC GAAGCCCACGAAACATCGGGCGCATCCACGCTTCAACCTC Clone Rv18 :::::::::::::Rv18T7.seq::::::::::::: AGCTTTTGGCAGGGTCTCCTTCGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACT (SEQ ID NO. 179) GGCCCGGGTGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCA CCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACGA TCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTC CCGTTGTGTCGAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCCCGGTGAG GTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACAGCCCGAATCGTCAC Clone Rv190 :::::::::::::Rv190SP6.seq::::::::::::: ATACTCAAGCTTTGTCACACCAACTGTTTCCACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGT (SEQ ID NO. 180) CGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCG CAGCTTGGGGATCGCGACTTCTATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGA CCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGT CGAGAGCAGCCCGCGCAGCAGATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAACCTGCTCGGTGT :::::::::::::Rv190T7.seq::::::::::::: CCTTAAGCCCCGCAGGGCCCGGCACGCGCGGTACCGCCCAGGTCGCCCAACAGATCGTCGATGTTCGCGTCGTCCGCC (SEQ ID NO. 181) TCGCGCACGTGGTCTGTCACCAGTCAACGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGA GCGGGCGACTGCAATGTCATGACACCGACGGCCGCCGATGGGCCCAGGGTCTGGCAGATTCGATCTGTGCGGCCAGTG CCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCACG GCACCACGGCCGCGGGCTGGCCGGTCAGATTCCAGACTTGAAAGTACGGAACCCGCTGCACCACCAGCAGCAACGTCG AAACTGCACCCCGGCGTTGGTAGGCGCCGATGCGGGACGGGCCGGTCGCGGCGCCTGGCGTCACAACTACGTCGACAT CGTCGAAGATCGACTGGATCGGCTGCTCACACCACTCGGCGGCCGCAGGCCGCCATCCGCCCTC Clone Rv191 :::::::::::::Rv191SP6.seq::::::::::::: AGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCG (SEQ ID NO. 182) AAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCG ACGGCGACGCGGTGGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTGCGC CGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCA GCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCA TTGGTGATGGAATCGGCGAACTTGGCCACCCGCTGGGTGTTGACATCCTCGACGGTGGGCAATTGCCCCCGGTAACGT TTGCCGCCT :::::::::::::Rv191T7.seq::::::::::::: CGGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCG (SEQ ID NO. 183) CACCGGCGACGTCGCGGTGGTCGACGGCAGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTC GGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCATCCGGACGTGGCGGAGGCGGCAGTCGT CGGGGTGCCCGACGATGATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAATGTCGATGCGGACGGGCT TATCAACTTTGTTGCCCAACAACTTTCGGTGCACAAGCGCCCGCGCGAGGTGCGTATCGTANATGCGCTGCCGCGCAA CGCCTTGGGGAAAGTGCTCCAGAACATTGCTGTCAGAAGCTGANCTACGCGAATTATCGTGTTACGCTGGA Clone Rv192 :::::::::::::Rv192SP6.seq::::::::::::: ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCCCCGGCGAGCCCAGCGAAGTCGCTACCGTGGCCGTGTTCTTGGCT (SEQ ID NO. 184) TCGGATCTATCCTCGTTCATGACCGGCACCGTGTTGGACGTGACTGGCGGCCGGTCCATATGACACCGAGATCATTGC CACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGA AATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCG GGCGTGCCGGCGCATTCGAGCTGGCGGTGCGCGCTGCCCAGCACCGTCATAGGTACTTGACGATGGTCCACGTCGGAC GAGCGCCTCCACGTCGCTGCCGAACGGTATGCATGGCGGCTACGATTCTC :::::::::::::Rv192T7.seq::::::::::::: CGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCAC (SEQ ID NO. 185) GCATGTACAGCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGC GGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCGTCACCGTCGGCGTTGGGCC CGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGCCGATGGCGCGAAACTCCC CATGACGAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCCGCCATTCGATCAAGTCAG CAATGGTGATCAGCGCCAGACCGTGCTCATCGGCGAACACCGCAATTCATCGGTGTTGCGCCATCGAGCCCTCATCTT TTTGGCTGACGATCTCGCAAATCGCCCCCGCGGGTTGCAGCCGGCAT Clone Rv193 :::::::::::::Rv193SP6.seq::::::::::::: ATACTCAAGCTTTGGGTGAAAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCCCGCCGCCCGGCATACGGCGG (SEQ ID NO. 186) CGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGAT TGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTAC GGTCACCGACCCGGTGACCGTNCTCGCCCGGTGCGCTCGGCCGATAAGTTGCACCGCCACCACCGCGACACCGTCTTG CACGCGGACCCACCCCCGGATCCGTTGTTGGCC :::::::::::::Rv193T7.seq::::::::::::: AGCTTGCTGGCATCCGCTCCAGTAGCGCCCCGCGCGTGGCTTCCAGCGCCCGCAGATGCTCCATGAGCCGGCCGGTCG (SEQ ID NO. 187) AGTCGGCGCCGGCGTTCACCGCCACCCGCCAGGAGCTGGCGGCCAGCATCTCCGCCTTCACGCATTGCGCGATCACAG AGAGAATATACGTCTCATATTCGTTGGAGGTCGTCGCAGGCAATCGGTCGATGACGGATTTGATGGCATCGAGCTGTG CTTCGGCGTAGCCCTCCAGCACGTCGGTATCGCTGTGGCGGTCCACGACGACCGCACCGGCGCGGCGGACAGCCGTCG GGTTGGACGNTGTGCGGCGATCAGTCCGGCCAGCTCCGCCTCGGGATCAGCGGC Clone Rv194 :::::::::::::Rv194SP6.seq::::::::::::: ATACTCAAGCTTGCTGCAGCTTCCTATGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGG (SEQ ID NO. 188) ACATCCTTCCCCTGAGACCCGCGGTCGAACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGC ACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAAAACAGCTGGTCGGCCGTTAGGA ACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGT AACNACNACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGGGGAAGAAGAAC TCAAAGCCAGTGGGTCGACGCGCAGGCCAACGC :::::::::::::Rv194T7.seq::::::::::::: AGCTTGACGCGGAGACGGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGA (SEQ ID NO. 189) TCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCA TTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTGCACGACAT TAAATGTCACGGTATTGTAGATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAA AAGCGTCCGTCATCTGTTTGACGGTGTCCCGTTGGGTATCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTT GGATGCGTGTTGCAGTGGCCTCATCTTTGATGATCAAATCGATGTGGCTCAGTCTTCCGGGCA Clone Rv195 :::::::::::::Rv195SP6.seq::::::::::::: ATACTCAAGCTTCGGCTCAGGCGGCGCTGCTGGTAAAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTC (SEQ ID NO. 190) GGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTC TTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCC GCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGG TCCGCTCGGATAGCGAGGTCAGCGAATTCNCNTGGCAGCTCCAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACNA AGGCNCAGGTTA :::::::::::::Rv195T7.seq::::::::::::: TGATCGCGCATCACCTGCTTCATAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATCAGCCAGCCTCTCG (SEQ ID NO. 191) TCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCC6CG ACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCG GGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAGGC CGCCGAGCAAGTCCGCCCGATCGCATACTCCAACCGGTTGCGGTACTGCAGGTTCAGCTGGCGTACTCCTCGTCGCGC TCGGCGAGGTCTTGCTCCAGCACGTCGCANACGGCAG Clone Rv196 :::::::::::::Rv196SP6.seq::::::::::::: CAAAGCGCGAACTGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAAATCAATTCCAGGCAGCTCC (SEQ ID NO. 192) CGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGA TCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCAGCGTGATCAGG CTCGGCTCGCGAGTTCCGGGAAGAAGTGGCTCCGCCTGATCACCTACCATCCGCCAGGATCTGCGTGTCTTCACCACG CCCGCCAAGGAGGTTGTTGTGGTGCTATCGACCGN :::::::::::::Rv196T7.seq::::::::::::: CCGGAAGCCGCATGATCAGCCAAGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCG (SEQ ID NO. 193) GGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGC GCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTNGTCGCCC GGTGCGCTCGGCCGAAAANTTGCACCGCCACCACCGCGAAACCGTCTTGCACNCCGGAAGCCACCCCCGATCCGTTGT TGGGCCAGGTTATTGGGT Clone Rv19 :::::::::::::Rv19SP6.seq::::::::::::: CCGGAACCGCCGACGGCACGGTATAACGCCTCCGCATATGGGTCGACAACCAGCGGGTCGGACTTCTGGGCTTCTAGC (SEQ ID NO. 194) GTTCGCGCNGTCGCGACAAACAGCGCGGTCGAACCGACACTCGTTGTGATGTCCTAGCTATCACGTTCGGTACGCACC CAATCGAGTCTAGCGCGGGTAGNTCAGCCCCGATCTCCANGCTCCGCCGAGCCAGGCGC :::::::::::::Rv19T7.seq::::::::::::: CTGGTTTATGTCCCGTTGAAGTTCCATCACCCGATGTGGCGGGAGCACTGCCAGGTCGATCTCAACTACCACATCCGG (SEQ ID NO. 195) CCGTGGCGGTTGCGCGCCCCGGGGGGTCGGCGCGAACTCGACGAGGCGGTCGGAGAAATCGCCAGCACCCCGCTGAAC CGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGATCGCGGTGGTTGCC Clone Rv1 :::::::::::::Rv1SP6D2.seq::::::::::::: CCGAGCAGTTGGGAATCGCTCTGCANCAAACCAATATTCTGCGCGACGTCGCGCGACGAGCTGGACCGATTAGCCGTA (SEQ ID NO. 196) CGCCTCCGNCTGGACGACACCGGGGCACTCGATGACCCCGACGCCTACGCTCGCAGGATATTGTTCGCCGGACCCCTC TCTAG :::::::::::::Rv1T7.seq::::::::::::: TATATAATACTCAAGCTTGCCGACGCCAACGCTCGCGCGATGTTCTTAGCCCGACCCGGCTCTTACATGGCACCGGTG (SEQ ID NO. 197) CCCCACACGTCAGCCTGTGACGTCCTGCACCGCGACTCTTTACATAGAATGTGGATTGCCGGATTGGGGATGTCCGGC ATCGCTCAATCTGTAGTCCGCGTTGTCCCGCGAGGGCCATGTGGATGGGGGGAAGGATCCGTGGCGTCCGGGATCACC ATGGGG Clone Rv201 :::::::::::::Rv201SP6.seq::::::::::::: ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGAGCCCAACGAAATCGCTAGCGTGGCCGTGTTCTTGGCT (SEQ ID NO. 198) TCGGATCTATCCTCGTACATGACCGGCACCGTGTTCGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGC CACGGTACGGAAATTCGTCCAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGA AATCGTCAATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCGAGGGTTTCTACACCACCGAGTTCATTCTCG GGCGTGCCGGCGCATTCGAACTGGCGGTGCGCGCTG :::::::::::::Rv201T7.seq::::::::::::: GCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTAC (SEQ ID NO. 199) AGCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGAC CCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCTTCACCGTCGGCGTTGGGCCCGGCGATC TCGCCGCGGACCAACGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGCCGATGGCGCGAAACTCCCCANGACAA GTCGGAATCCGCGCCTCGGCGAACCGCTCAATGTGCCTCTCGTGCTTGCGCCGCCATTC Clone Rv204 :::::::::::::Rv204SP6.seq::::::::::::: TGGTCCGTGTGCGCATACCAATACAACGCGCCGGGCACCTGACGCGGCGGCCGCAACCAATCGGTGGCCATCGCCATC (SEQ ID NO. 200) TTCTGCTACCCGGTCAACGGACGCACCTTCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTGCGTCCCATCGAT CCGGTCAAC Clone Rv205 :::::::::::::Rv205SP6.seq::::::::::::: GGCGTGTTGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCC (SEQ ID NO. 201) GACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTAC TTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTAC ATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCT GGTTCAACCAAACTTGAAGGTGATTGTTTACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCCCCGCCCAA :::::::::::::Rv205T7.seq::::::::::::: CGTCCGTGNCCCCTCAANCGCGTGNNGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATGGACACTTCTACCAACT (SEQ ID NO. 202) GCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGANCG GCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGA CGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAAACACCGCCAGCTCGGCTTCG AGTTCGGCACTTTCAGTGACCGGTTCAACCGGCTCGAAGAGGCGCTACAGATCCTCCAGCCAATGGTCAAGGGTGAGC GCCCAACGTTTTTCGGCGATTGGTACACCACCGAATC Clone Rv207 :::::::::::::Rv207SP6.seq::::::::::::: CCGCTTCCGTGTAACCGAGCANNGCGAGCGANCTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACG (SEQ ID NO. 203) CTCAGCGCAAGAAGAAATATCCACCGTGGGAAAAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAAT AAACTGTGATAATCAACCCTCATCAATGATGACGAACTATCCCCCGATATCAGGTCACATGACGAAGGGAAAGAGAAG GAAATCAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGC AGGCTGAAGGAAACAGCAAAACTGTGACAAATTACCCTCAGTAGGTCAGAACAAATGTGACGAACCNCCCTCAAATCT GTGACAGATAACCCTCAGACTATCCTGTCGTCATGGAAGTGATATCGCGGAAGGAAAATACGATNTGAGTCGTCTGGC GGCCTTTCTTTTTCTCAATGTATGAGAGCG Clone Rv209 :::::::::::::Rv209SP6.seq::::::::::::: TGACACCCAACAGAGGGCACTTAAGATGGCAATGCGGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGATGCCGAG (SEQ ID NO. 204) GGAGAACAATGCGAGCACGGCCGCTGACNTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGCGGGCTGCN AGGCCCGANTCNAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGTCTCGACCGCAAGCGCGACCTCAGCCGCAGC CGGTGGAGCTACTGCTGCGCGCCATCACGCC :::::::::::::Rv209T7.seq::::::::::::: ACGGGCGACGCTGAGGTGGGCCCGCGGCTATTCATGCTGTCGTCCACGTCCAGCGACGCACTCCGCCAGACGGCCCGC (SEQ ID NO. 205) CAACTAGCCACCTGGGTGGAAGAACACCAGGACTGCGTGGCGGCCTCGGATCTGGCCTACACGCTGGCGCGTGGCCGC GCGCACCGGCCGGTGCGCACCGCGGTGGTTGCCGCCAACCTGCCGGAGCTCGTCGAGGGTTTGCGCGAGGTGGCCGAC GGTGACCCCTCTATGACGCGGCGGTGGGACACTGTGATCTAAGACCGGTCTGGGTCTTCTCCGGGCAAGGGTCTCAGT GGGCGGCGATGGGCACCCAATTGCTCGCCAGCGAACCAGTGTTCGCGGCCACCATCG Clone Rv20 :::::::::::::Rv20SP6.seq::::::::::::: ATACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATAC (SEQ ID NO. 206) TTGAACCTGGTCTCGTTCGGCAATAACTCGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCC GACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCGACCAGCACGCTCAACCCGTACACCAACCCC GACGGCGCGCTGGCCCGGCGGAACGTGGTCCTCGACACCATGATCNAAAACTTCCCGGGGAGGCGGAGGCGTTGCGTG CCGCCCAGGGCGAACCGCTGGGGGTTCTGCCGCAGCCCAATGATTGCCGCGCGGCTGCATCGCGGGCGGCGACCGCCA TTCTTCTGCGAATACGTCCAGGAGTACTGTCTCGGGGC :::::::::::::Rv20T7.seq::::::::::::: AGCTTATGTGGCCGCCCACCTACCTTATCTAGCCTAGCTAACTAAATCCAGTGCCGACAGTGCGCGGCTGGCCACCCA (SEQ ID NO. 207) GCATGAGGTTATGACCACGGCATATGCCAGCGCGCTGGCGGCGATGCCGACGCTGACCGAGTTGGCCGCTAATCACAC CAGCCATGCGGTGTTGCTGGGAACGAATTTCTTTGGAATCAATACGATCCCGATCGCGCTCAATGAGGCCGACTATGC GCGGATGTGGATTCAGGCGGCCACCACGATGAGTATCTATGAGGGCACCTCCGATGCGGCGCTGGCGTCNGCACCGCA AACCACACCGGCTCCGGTACTGTTCAACGGCGGTGCTGGCGTTTGCCAGCGCCTGCCGGCGATCTC Clone Rv214 :::::::::::::Rv214SP6.seq::::::::::::: ATACTCAAGCTTGCCACCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGTGTTGCTGCTG (SEQ ID NO. 208) GTTGCGGGCTATGAGAGCACTGCTCATATGATTAGCACNTTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACA CTCCTTGCGCAGCAACCAGACCTGATCCCGTCGGCGATCGAGGAGCACCTCCGCTTTATATCGCAATCCAAAACATCT GCCGCACAACGCGCGTCGACTATTCGGTCGGTCAAGCGGTCATCCCGGGA :::::::::::::Rv214T7.seq::::::::::::: CCGGGGTAGAACGATGCGATCTGGGCCATGTCGACATCGGTGGTACAGGTAAACCGCGCCGTGTGCGCGGTCTCGGAG (SEQ ID NO. 209) ATCAGAACGTGGTCGCAGTTGACACCGCGGGCTTTCAGCCAGTCGCGATAATCGGCGAAGTCGGCGCCTGCCGCCCCA ACTAGCGCGACCTCGCCACCTAGCACACCGATGGCGAAGGCCATGTTTCCGGCCACGCCGCCGCGGTGCATCATCAAC TC Clone Rv215 :::::::::::::Rv215SP6.seq::::::::::::: ATACTCAAGCTTGGCGGCAACGCCACTACCGGGCTCACCAGGTCCTGTGCCGCCACCGCCGGCGCCGAAAGCACCATC (SEQ ID NO. 210) AGGTCGTAGTTGTCTGGACGTTCGACACCGTAAGCGAACACAATGCCGCCGCCCATGCTGTGCCCGAGCACGATGCGC TTGCACCCGGGATATTCCCGGGTGGCGATCCCAACGAGGGTGTCGAAGTCAGCGGTGTATCTGAGATGTCTCTCACTA TCATCCGTTTGGCACCCGAGCGGGCATGCCCGCGGGGGGTCAAC :::::::::::::Rv215T7.seq::::::::::::: GTCGACGGCATCAAGGTCCGCAGTGATGGTGTTCATCTCACCCAGGAAGGCGTGAAGTGGCTGATACCGTGGCTTGAG (SEQ ID NO. 211) GATTCGGTGCGGGTCGCCAGTTAATCCGCCGTGTGCTCCGGATGAGCGCGACGGTAACCCTGGAATTGTGCTGTGTGC TGGCTGTGTCGTTGTGATGAGCCTGTCTAAGTGGTGCGTAACCGTTTGACGAGCCGCGGCCTCGCTGCAAACATTGAA GCCCGCACGTCTGGGTTTGTATTTACACAACGAGGGCGCTCCCCGATCTGGCGCGCGCAACGAGGTGCNCACTATCCA TTCGAGGTGAACTGGACTCCTTGATGCTCATGCCGGTGCGGTTTTGTC Clone Rv217 :::::::::::::Rv217SP6.seq::::::::::::: ATACTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTGGCGATGCCCAGCAAGGA (SEQ ID NO. 212) GTCATCGCCGCCGAGCTTGGCCAGGATCTTGTCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTA GACACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGCGTTACAAACTCGCTGACGTCGT CGCCGCTGTCGCGAATGCCCTC :::::::::::::Rv217T7.seq::::::::::::: NGTCAAGCCGAGCATGCGCGAGGNAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCG (SEQ ID NO. 213) CCACAACGGGAAGATCGCCTTGAGCGTCGCTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATC ATGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCGACGAGGTCTCGGTGCCGCCGCGGCCAGGGGACC AGCAGTGACGATTCCAGGCGCCGTCGGG Clone Rv218 :::::::::::::Rv218SP6.seq::::::::::::: CGATAATCGCTTCCGGTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGACACCAGATACTCTT (SEQ ID NO. 214) CGACCGAACGCCGGTGTCTGTTGACCAGTCAGTAGAAAAGAAGGGATGAGATCTCCCCGTGCGTCCTCAGTAAGCAGC TCCTGGTCGCGTTCATTACCTGACCATACCCGAGAGGTCTTCTCAACACTATCACCCCGGAGCACTTCTAGAGTAAAC TTCCCATCCCGACCACATATAGGCTAAGGTAATGGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAAT CCACCATCGGGGCCGCTGGTGTCN Clone Rv219 :::::::::::::Rv219SP6.seq::::::::::::: NAATACTCAAGCTTTCTCGTGATTACCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAATCACCGCGTGGCCACAAA (SEQ ID NO. 215) CGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTC GCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTATCACCCTGGCCGGGCAATGATCTGCAGCGTCGCCGC GGGTAGTGNCCGCCCGGGCGGCTAC :::::::::::::Rv219T7.seq::::::::::::: CCAACTAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACG (SEQ ID NO. 216) ACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGATGTTGGTGCTCGGCGGCTACTCCCAGGGTG CGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGTCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACG ATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGGCCGCGCTGGCGGGCTGATGATCGCCCTGACCCCTCAA TTCGGGTCCAAGA Clone Rv21 :::::::::::::Rv21SP6.seq::::::::::::: ATACTCAAGCTTGCTGCAGCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGG (SEQ ID NO. 217) ACATCCTTCCCCTGAGACCCGCGGTCGAACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGC ACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAAAACAGCTGGTCGGCCGTTAGGA ACTGAATTGAAACTCAACCGATTTGGTGCCGCCCGTAAGTGTCCTGGCTGCCGGTGCGCTGGTGTT :::::::::::::Rv21T7.seq::::::::::::: AGCTTGCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCCGCGACGGAGCC (SEQ ID NO. 218) TTTATCGACATCCGTTCGGGCTGGCTGACCGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGA GCCGAGCGCCGTCAGATGTACGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTTTCACGACCTGACCATCGAAGAT CCGCCGCATCCGCAGCTGGCGCGGATGCGCC Clone Rv220 :::::::::::::Rv220SP6.seq::::::::::::: AATACTCAAGCTTGCGCACGACCAGGACGTCGAGTGGCGCTTGCAGTGACTTGGCGACCTCAAAGGCCACCGGTACCC (SEQ ID NO. 219) CGCCGCGCGGCAAGCCAAGGACNACNACGGCCTTGCCGGATAGCTGCGCCAGGCGTTGCGCCAACTGGCGTCCAGCGT CGCCACGATCGTCAAAGAGCTTCATCTGCCGAGTGTGTCGCCATCTCATGGCTCCAAATATGGAATTAGGTCCCTGGG CCGACTGACGACAGTCCCTCAGCGACCGGATTGCGCATCCCGCCTTGTACGCTGCTCCGCAAATCCCGGGCTTGCCTC CGCGGAAGCGAACTCGGCGGCGCTACGGTGGTGGCTCACTTCGGCCGTGC :::::::::::::Rv220T7.seq::::::::::::: GGTTGGTGCGGTCCACCTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATCTATGGGT (SEQ ID NO. 220) CGTGGTTACTCAGCGGGCCGAAGCTGGCCCTCCCACGGGTAGGGCCCTATTCGACGGTGATGCCCATCGACCGAGCGG TACCGGCGATGATCTTGGCCGCAGCGTCGACGTCGTTGGCGTTGAGGTCCGTCTTCTTGGTCTCGGCGATTTCGCGGA CTTGATCCCAGGTGACTTTGGCGACCTTGGTCTTGTGCGGCTCCGCCGAACCCTTCGCCACACCAGCGGCCTTAAGCA GCAGCTTGGCGGCGGGCGGCGTCTTCAGCGTGAAAGTGAAGCTACGGTCTTCATAAACGGTGATCTCCACCGGGATGA CGTTGCCGCGCTGGTTCTCCGTCGCGGCGTTGTACGCCTTGCAGAACTCCATGATGTTGACCCGTGCTGACCGAACGC GGGGCCCACTGGCGGGGC Clone Rv221 :::::::::::::RV221SP6.seq::::::::::::: ATACTCAAGCTTTTCGACCCGCAAGCCGGCGGTGCCCCTCCTCGTTCCGCTGCCCGGTCTGCTCGATCGGTTCGGGGT (SEQ ID NO. 221) CGCCGCGCTAGGCCCAATTGCCCGGCTCCTCCTCGGGCCGTTCCACAACCCGCATCGTCGCCGGGCTAGGTTCAACCC ATGCCGGTAAACCCCAGGACGCCAGTGCTGATCGGCTATGGACAGGTCAACCACCGAGGCGACATCGACGCCNAAAAT CAGTCCATCGAACCCGTCGACCTGATGGCCNCCGCGGCCCGGAAAGCCGCCGAGTCCACCGTGCTCGAAGCGGTGGAT TCCATCCGTGTGGTGCACATGCTGTCGGCGCATTACCGGAATTCCCGGGCGTCTCCTCGGC :::::::::::::Rv221T7.seq::::::::::::: NCCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGC (SEQ ID NO. 222) TGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCC AGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGGCCGGGTGTTGGGGTG TTCGGCAACGGCAACCAAGTTGGTCCACACTGCCGACGGGCGCCGCAAATCCGTTCACCGAACCAGGCCGCCNAAACA ATTCCGCCCGATCCCATAT Clone Rv222 :::::::::::::Rv222SP6.seq::::::::::::: ATACTCAAGCTTGTCGGGATCAATCTCGAGGGCATCCACGCACGAAAAGTAAACTCTATCAAGCTTTTTGACGACACC (SEQ ID NO. 223) CACGGACGCCCCATATATGTTCGGGTGGGCAAGAACGGTCCCTACCTGGAACGTTTGGTGGCCGGCGACACCGGTGAG CCCACGCCGCAGCGGGCCAACCTCAGCGACTCGATTACCCCGGACGAACTGACTCTACAGGTGGCCGAAGAGCTCTTT GCCACACCGCAACAGGGACGGACTTTGGGCTTGGACCCAGAAACCGGCCACGAAATCTTTGCCAGGGGAAGGCCGGTT TGGGCCTTATGTTACCTATATCCTGCCGGAACCTGCGGCTGATGCGGCCGCGGCCGCTCAGGGAN :::::::::::::Rv222T7.seq::::::::::::: AGCAGCTAGCCGCGCTCGCCGCGCTGGTCGGTGCGTGCATGCTCGCAGCCGGATGCACCAACGTGCTCGACGGGACCG (SEQ ID NO. 224) CCGTGGCTGCCGACAAATCCGGACCACTGCATCAGGATCCGATACCGGTTTCAGCGCTTGAAGGGCTGCTTCTCGACT TGAGCCAGATCAATGCCGCGCTGGGTGCGACATCGATGAAGGTGTGGTTCAACGCCAAGGCAATGTGGGACTGGAGCA AGAGCGTGGCCGACAAGAATTGCCTGGGCTATCGACGGTCCAGCACAGGAAAAGGTCTATGCCGGCACCGGGTGGACC GCTATGCGCGGCCAACGGCTGGATGACAGCATCGATGACTCCAAGAAACGCGACCACTACGCCATTCAAGCGGTCGTC GGCTTCCCGACCGCACATGATGCCGAAGAATTCTACAGCTCCTCCG Clone Rv223 :::::::::::::Rv223IS1081N1400.seq::::::::::::: CGCGACTGGCTCCCCGGNCGGCTGCTCGGGTCCGCCGATAGAGACCGGGATCTCGCCCGACGACGGGCAGCCGGGTTG (SEQ ID NO. 225) CGTGGGACGGGGCGGGGGTCGGGCAGCCCAAGCAACGGGCTAGTCCCCGAATCCTACGGAGCCGTCACCTACGCCTAC GTAATAGTAGCTATCAATAACAGTTGACATACGCAACGATCTGTGAGATCAATATTGCCTGACGCATGTCAAGACAGG CGTCAAGACAGGTGTCAATAATTCGCTCCGCTGGTGACGGTAACCGGTCGTGCGGGTGTGTGACGCCTAAGGAAGGAG TGTGGGTGGTGACGCTGAGAGTGGTTCCTGAGGGTTTGGCGGCCGCCAGTGCGGCGGTGGAGGCGTTGACCGCACGGC TGGCCGCCGCACACGCTGGCGCGGCGCCGGCGATTACGGCGGTGGTGGCGCCCGCGGCGGATCCGGTGTCGTTGCAGA ATGCGGTGGGGTTTAGCGCCTTAAGTAGCCAGCATGCCGCGATCGCCGGCGAAAGGGTCCAAGAACTGGGT :::::::::::::Rv223SP6.seq::::::::::::: ATACTCAAGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCAACAC (SEQ ID NO. 226) GAACGGCCGGACGACGTGGGCCAGGGTCGCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAACGACAGGCTCCGG TGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCC CTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAATGCAATCCCGCAAAATGCAATGCGTCNAGCTATTT CTCACACCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGAATTTGTTCTCCCGCTCCGCAT CATGCTTGTAATCGTTTGGAAATTCATCCTCATATGCCTCGATCGCTTCATAGGGTCCAGGCCCAAACCCGGGCAGGA CTGGGTGGCCGTTGATGTTGGAATCCTCCACTACTAGGTATTCACCGGC :::::::::::::Rv223T7.seq::::::::::::: GTCTCGATCATGGCCAAAGAGCTCGACGAAGCCGTAGAGGCGTTTCGGACCCGCCCGCTCGATGCCGGCCCGTATACC (SEQ ID NO. 227) TTCCTCGCCGCCGACGCCCTGGTGCTCAAGGTGCGCGAGGCAGGCCGCGTCGTCGGGGTGCACACCTTGATCCCCACC GGCGTCAACGCCGAGGGCTACCGAGAGATCCTGGGCATCCAGGTCACCTCCGCCGAGGACGGGGCCGGCTGGCTGGCG TTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCGGCGACGCCCACGCCGGCCTGGTGGCC GCGATCGGCGCCACCCTGCCCGCAGCGGCCTGGCAGCGCTGCAGAACCCACTACGCAGCCAATCTGATGGCAGCCACC CCGAAGCCCTCCTGGCCGTGGGTGCGCACCCTGCTGCACTCCATCTACGACCAGCCCGACGCCGAATCAGTTGTTGCC AATATGATCGGGTTCTCGAC Clone Rv224 :::::::::::::Rv224SP6.seq::::::::::::: ATACTCAAGCTTTCGTCAGTTCATGGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCA (SEQ ID NO. 228) ACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAAATGGCCAGCGCGTGCCGGG CCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCAAGATCTTCACCGCCGCACCACTGCCCGGCC TCGGGTTCACGCATCCGTTTGGCCGCCGCC :::::::::::::Rv224T7.seq::::::::::::: GCCCCGTGTAATTTGGGATGGGCAAAAAGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCT (SEQ ID NO. 229) AGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGAT GGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCTCCGCCCGGGCCGC Clone Rv225 :::::::::::::Rv225SP6.seq::::::::::::: ATACTCAAGCTTCCTTTGACCGAACGCGTCCACCGCACCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTG (SEQ ID NO. 230) TTGGCGGCGTCGCCGTATTGTGCGGGCCAGCCTTGTGCGGGGGCCGCTTCTACCCACAAGTCGGCACTTCCGCAACCG CCCAGCTCGACCGCGAATTACGGCGGCCGCAACGGCCGCCGGAAGGCGTCACGCAATCGCTTATCCTTTCCAGGTTCC CAAATCCTCCGCTTACTTGGGTCCTTCATCGG :::::::::::::Rv225T7.seq::::::::::::: GGCAGCGGCGACAACCGGAACGTCCGCACGGTGCTCAATCACGGGTGCACGGTGTGCATCAGAATGGCGGGGGTTCGT (SEQ ID NO. 231) TGTCGCGGTGAGGCGTTCGGCGAGGAGGTAGTGTCTACCCCTTGCCCGCGGGTTCGTGCGGACTGAAAGGGATTTCAT TGGGAACCCACGGCTGCGTATCGCAGGGCCTCGGTGACGTCTGCTTCCTCNAGCTCAGGAAGTTCGGCGAGAATCTCG GTGGATGTTATTTGGTCCGCCTAC Clone Rv226 :::::::::::::Rv226SP6.seq::::::::::::: ATACTCAAGCTTTCTCGGCTTCTCTGATAGCCTGAGAAGAAACCCCAAGTTAATCCGCTGCTTCACCTATTCTCCAGC (SEQ ID NO. 232) GCCGGGTTATTTTCCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAATGGCGATAGCCTTCGTCATTTCATGACCAG CGTTTATGCACTGGTTAAGTGTTTCCATGAGTTTCATTCTGAACATCCTTTATTCATTGTTTTGCGTT Clone Rv227 :::::::::::::Rv227SP6.seq::::::::::::: ATACTCAAGCTTGGTGACCGGCACCGCGATACGTTGCGGCAGGCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCC (SEQ ID NO. 233) GTCGAACACCATCGCCAGCGCGGCTTCCACATCAACGACCATTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGAT GAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCGTATCACATCGATTCGACCATCGCGCGGCGCGCGTTGCCGAGGGC GAACGAGGCGGTGCCCAACCGCAATCTGTTTGGTCAGCTCCCTCATGCGGGTTGATTCCTTGCCGTCCGGACGGGCCC GCGTCATGCGCTCGGTTCGCC :::::::::::::Rv227T7.seq::::::::::::: CCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGTGAAATCGATGGCCAGGTCGAGGTCCCATGCG (SEQ ID NO. 234) CGTGGGCCATTGATGCTGATCGCCAGGACGTCAAAGATTTGGTCCGGCGTCAGCTGGGCGAAAAACGTGGGCGCCGGG ACTTGCCCGGAGCTGCCCGGGTTCCCGTCGCGCAGCTCGGCGGCCCCGGTCAGAAAGAAATTGCGCCAGGTCGCACAC TCCGCGCCGTAGGCCAGCTGCTCCAGGGTGTCGGCATAGAGCCCGCGGGCCGCAGCGTGCTCGCTGTCGGCGAACACC GCATGGTCGAGAAGCGTTGCCGCCCAACGGGAAATCACCTGCGTCGAAAGCTTCGCGGGCCAGCTCCAGCACTCGGTC GATGCCACCCAACGCGT Clone Rv228 :::::::::::::Rv228SP6.seq::::::::::::: ATACTCAAGCTTGCGGATGTTACCCCTGACAGCGTGAACTATGTCNAAACACACGGCACCGGAACGGTGTTGGGGGAC (SEQ ID NO. 235) CCCATCGAGTTCGAGTCGCTGGCGGCCACTTATGGCCTGGGTAAAGGCCAGGGCGAGAGCCCGTGCGCATTGGGGTCG GTCAAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATTCATCAAGGCGGTGCTGGCGGTGCAACGT GGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCAACACGTCGGGGACGCGGCTGTTCGTGCCG ACCGAAAGCGCCCCGTGGCCGGCGGCTGCCGGTCCACGCAGGGCTGCGGTGTCATCGTTCGGCCTCAGCGGGACCAA :::::::::::::Rv228T7.seq::::::::::::: CCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGCTGCTGCGCGCTCCCCAGTAGTGCA (SEQ ID NO. 236) CCTTCTTGACGCCTCGAAAAGGGGAGTCCGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCGGTGAC CGGTCTCCTCGAGTATCTCCCGCACCGCCCCCACCGGTGCGGTCTCGCCCGGATCCACTTTGCCCTTGGGCAGCGACC AGTCGTCGTAACGGGGGCGGTGAATGACAGCGATCTCGACCGGCCCTTCCGAATCGGCACTGCCGGGTCGCCAGAACA CCGCACCGGCGGCGTACACAATCCGGCCCGCCCAGCGCCGGCGGGCGGACGANTTCTGGATCGACACCTCAACTCCTG CAGGTCAATTCGGCCAAGCTGCTCGCGGTCGTGGATGTGGTC Clone Rv229 :::::::::::::Rv229SP6.seq::::::::::::: ATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCCGGGCCCGG (SEQ ID NO. 237) GCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCG GAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCTATACCGATTTGCGCATCCGCAGC CGCACCCTGGTCGTCTCGTACCGTGCCCTACCTCTGCTTGTCGGGCGGGGCCA :::::::::::::Rv229T7.seq::::::::::::: TCCGTACGGCCCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATA (SEQ ID NO. 238) GCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATA TGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGG GCCACCATCCATCCACCAGGTCTGCGCGAATCACCCGC Clone Rv22 :::::::::::::Rv22SP6.seq::::::::::::: GGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAG (SEQ ID NO. 239) CCAAATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAA CTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTGCACGACATTAAATGTCACGGTATT GTAAATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGTCCGTCATCTG TTTGACGGTGTCCCGTTGGGTNTCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTGTTGCAGT GGCCTCATCTTTGATGATCA :::::::::::::Rv22T7.seq::::::::::::: GCCTGGCCCAGGTGAAGGCCGACCTCGACGCCAAAGCCGCTGATCCGGCACATGAGTCGGTGGACTGGGACTTGAAGT (SEQ ID NO. 240) CGCTGCGATGGGCGTGGAACCGAGCCAAAGATGACGTGGCGCCGTGGTGGGCCGAGAATTCCAAGGAGTGCTACTCGT CGGGGTTGGCCGATCTGGCCCAGGGCCTGGCTAATTGGAAAGCTGGCAAGAACGGGACCCGCAAAGGCCGGCGGGTGG GCTTCCCGCGATTCAAATCCGGGCGGCGTGATCCTGGCAGGGTGCGGTTCACCACCGGCACCATGCGCATAGAGGATG ACCGGCGCACGATCACGGTCCCGGTGATCGGGCCGCTGCGGGCCAAGGAGAACACCCGCCGGGTGCAACGCCACCTCG TGAGCGGGCGCGCGCAGATCCTGAACATGACCTTGTCGCAGCGGTGGGG Clone Rv230 :::::::::::::Rv230SP6.seq::::::::::::: TAACTCAAGCTTCAAGTCCGCNGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCCCGCCGCGGCGTTCG (SEQ ID NO. 241) ACCCGACAGCTGGTACCGCACCGGCGACGTCGCGGTGGTCGACGGCAGTGGGATGCACCGCATCGTGGGACGCGAGTC GGTCGACTTGATCAAGTCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCATCCGGACGT GGCGGANGCGGCAGTCGTCGGGGTGCTCGACTATTATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAA TGTCGATGCGGACGGGCTTATCAACTTTGTTGCCCAACAACTTT :::::::::::::Rv230T7.seq::::::::::::: CCATGTCGCCCAACATATCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAACGTTAACGCC (SEQ ID NO. 242) GCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGAGCGGGCGACTGCAATGTCATGACACCGACGCCGCCGA TGGGCCCAGGGTCTGGCAGATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGT TGAACCGACATGGGCATGCCGTCGCCGTCGAAGTCCCACGGCACCACGGCCGCGGGCTGGCCGGTCAGATTCCANACT TGAAAGTACTGAAGCCGCTGCACCACCAG Clone Rv231 :::::::::::::Rv231SP6.seq::::::::::::: CGAAAGCGTGAAACAGCTCGCGGCAGCCCCCGACGTGCTGCGTCGGATAGCCGGCGGGCGAAGATCAATTCCAGGCAG (SEQ ID NO. 243) CTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTG CCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCAGCGTGAT CNTGCTCNGCTCTNTANTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACA ACGCTCGCCAAGGAGGTTGTTGTG :::::::::::::Rv231T7.seq::::::::::::: TCCGCCACGCTTCGCGCCGCCCGGCATACGGCGCGTACCGATCTCCGCGTCATACACCGCGGGTAATCGCCGACGGTG (SEQ ID NO. 244) CCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGC TCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGA AGCTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACCCACCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTC ATTCCATTTGACGGGACGCCGACCC Clone Rv232 :::::::::::::Rv232SP6.seq::::::::::::: CATTCTTTAACAGTTGTTTTGGGCTCGGCATGGTTAGCCAACGTTCTGCGGTCCACCATATCATCTTGGTCCGGTAGC (SEQ ID NO. 245) GCTCGTCCGGGGTATGCTGCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAGAACCGCCACCGGTCCAGCGCGTG GGCCGNCGCGGTCCCATCACAAACTGAACCCCCAACAGGGACATGCTTATCGGTAGGGCGCGCGCCAAGGCGGCAGCA ATCGCATCACTGCGCTCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACCACCCCGAATGGCGCCCGGTCAT TGATCATCTGGCGCACCGCGGATAA :::::::::::::Rv232T7.seq::::::::::::: CGGTGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCATGCCGATGCCGCGGCCTCGTGGCCACGCATGTACAGCAC (SEQ ID NO. 246) CACGCCGCGCCCCTCACGGGCGAACATCGCCAGCGCGGCGTCCAGCTGAAGCCCGCAATCGCACCGGCGTGACCAAAC ACATCGCCGGTCAAGCACTCCGAATGCACCGGACCAGCACGTCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGC GGACCATGCGCGACATGTTCCACGTCCTCGTANATGCTGGTGTAGCCGATGGCGCGAAACTCCCCATGACGAGTCGGA ATCCGCGCCTCGGCGACCCGCTCAATGTGCT CloneRv233 :::::::::::::Rv233SP6.seq::::::::::::: CGGCATCTGGCGGCTGAACCTGTTCTTGGGCAACATGCCGAGGATCGCCTCTTCCACCACGCGGTCGGGGTGGCGTTG (SEQ ID NO. 247) CATTACCTCACCGATGGTGCGCTTGTGCAGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTT GTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACGATNACNAATCACCGCCANCGACATTGGGGGCGAACGTCGGC TCGTGCTTGCCGCGCAGCAGGCTGGCCGCCGCGACGCAAGGCGCCAACCACCACGTCCGTGGCGTCGATGACGTACCA CCATCGCGTGGTGTCACCCGCCTTGGGC :::::::::::::Rv233T7.seq::::::::::::: GCGGCAAAAATTGAAGCACTCNTGGCCACTNCCGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGG (SEQ ID NO. 248) CCCGAACGTACTGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCTGGGATGGCAGCAACCTGTTAGCAC CCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCCCCGGGCGGCTACAGTCTGAAACGCGATGACCATC GATGTGTGGACGCCGCATCCGACNCAACGGTTCCTACACTGTGATATGTTCGCCTCGCTGCGCCGGTGGACGGTGGGT CTATCCCGGA Clone Rv234 :::::::::::::Rv234SP6.seq::::::::::::: CGCGTTGAACTGAAGGGGTGCCGCCCGGCTCGAGCAGGCAAGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGG (SEQ ID NO. 249) TGACTGCCCGCCGCCGGCCAGCTCGGCTCAGTGTCCGGCGTTGGTCGCCGCGGCGACAATCTTGGCGTCCACGGTGGT CGGGGTCATGCCCGCGAGCAGGATTGGCGAGCGGNCGGTCAGCCGGGTGAACTTCGTCAAGAGCTGACGCTGCGGTTG GGGAGGCGAATCATGGTCGGTGCGTAGCCTCGACTAGGCCCGGG :::::::::::::Rv234T7.seq::::::::::::: TGACAACGCGGCGGCGATTACCCCGCTACCGCAGCAGCATGACGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGC (SEQ ID NO. 250) GTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGTGCCACCCCCTGCAGCGCGACACCGAC GATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGATTGGAGCTGGCGTATATGGCGGCGATGGTGACGAT GGTCATCGCCTCTTACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACCANATCC CCGGGGTCAACAGGTTGACCATCC Clone Rv235 :::::::::::::Rv235SP6.seq::::::::::::: CGCGGACATCCCGAACGAGGACACGCGACCGCTTCGGTGTGTGATCTATCAGGGCTCGCACCACGCGCAACCGCTTCC (SEQ ID NO. 251) GGCTACCTAGACGCGGT :::::::::::::Rv235T7.seq::::::::::::: GCATGCGGGTGATGCCGTTCTCAGTGCGCAACAGCGTTCGACGCGGCATACCCAGCCGCACATGCCGTGCACGCCGGN (SEQ ID NO. 252) GCCGGGGCGGGAATCT Clone Rv237 :::::::::::::Rv237SP6.seq::::::::::::: CTCAAGCTTCAGNCCNTCTAAGCGGTCTGCGCGGCGATCGCAAAGATCGCCCTTTGCCGGCGTTGGGGGCTTCTGCTC (SEQ ID NO. 253) GGGGGTGTTGTACACCTTCTCGAACACCTCGGCACCGACACCACCACCGTCGGCTTGAACACCGCCAACATCGGCAGC ANATCTTGATGTCCTGGTGAATCCACGGTGACTTTGGAGTGGAAGGCGGCCATACTGATCGCGCGCGCCACCACATGA GCTAGCGGCAGGAAAACCAGCAGCCGCTCACCCTTGCGCAGCAGCGTCGGGTGATATGCCTGGCGCCC :::::::::::::Rv237T7.seq::::::::::::: AGTCGAANGTCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGGCGACGGTGTCAGTGCAGAACAGCGGAAAC (SEQ ID NO. 254) TGGTGGCGCCCTAGGCGAGCGAACGCTCACAAACGGCGGTGACCGCTTCTGGTCGTGCACCATCGAGCCGTGCCCAGC CCGGCCGCGTGCCGTCAGCCGCATCCACTGGATGCCCTTCTCGGCGGTTTCAATCANGTACAGGCGACGTTCGCCACC ATCGTGCCGGGGCACGGTTAGCGAGAAACGCCGACTTCACCGATTGCCTCGGTGATGxxxxx Clone Rv23 :::::::::::::Rv23T7.seq::::::::::::: AGCTTCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCGGCGACGGAGCCT (SEQ ID NO. 255) TTATCGACATCCGTTCGGGCTGGCTGACCGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGAG CCGAGCGCCGTCAGATGTNCGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTTTCACGACCTGACCATCGAAGATC CGCCGCATCCGCAGCTGGCGCGGATGCGCCGGCGGCTCAACGACATCTACGGCGGCGAACTGGGTGAGCCCTTCACCA CCGCCGGGCTGTGCTACTACCGCGACGGCTCTGACAGCGTCGCCTGGCATGGCGACACCATTGGTCGCGGCAGCACTG AGGACACTATGGTGGCGATCGTCAGCCTCGGCGCCACCCGCGTCTTCGCGCTGCGGCCGCGTGG Clone Rv240 :::::::::::::Rv240SP6.seq::::::::::::: AGCTTCAGCTGATACTCGACCAGCCCCACTCGGGCCAATACGTGAATGTCTAGCATCTTCACCCGTTCACGGGCTANT (SEQ ID NO. 256) CGAGTAGTAGACATTGATTAGCCTGAACGTACCTCCGACGCCAGCTGACGAACGGGTATGACGGATGGATTTCGTGGT GTCGCGCCCGAGGTCAATTCGTTACGGATGTATCTCGGGGCCGGATCGGGGCCGATGTTGGCGGCCGCGGCGGCCTGG GACGGACTATCCGACGAACTGGCGGTGGCGGCGTCGTGGTTTGGGTCGGTGACCTCGGGCCTGGCGGATGCGGCGTGG CGCGGCCCGCGGCGGTTGCGATGGCNCGCGCGGT :::::::::::::Rv240T7.seq::::::::::::: CTGGTCATGGACGTTGCTCCGGTAGTGGCTCACTGCCGATCCTCCTCGTTGAGAGTGCCACCTCAGGGTTGGGTAGGG (SEQ ID NO. 257) TTGGGTACTCGAAACCAAGTTACCCACCAGTAACACCGTCAAAATATATCCGTTGCATAGGTCAATGCAAGTTGATGT GAGCTACATTGCACCAACTAACTAACCAACCGGTTGGGTTAGCGGTGATCCTGGCCGTGTCGGTCCTCTCACCTGCGG TGATAGCGATCAAATGAAGAATATGCGGAGTCTAGGGCGGCAGCGCCTGGCANCGTAGATCATCGGCTCACGCGGATG CGGCCTCTTGGTACGGACATGCGCGCG Clone Rv241 :::::::::::::Rv241SP6.seq::::::::::::: CTCGTGAGTAGCACCCCTGTAATTTGGGATCGGCAAAAAGGCGAATCACCGCGTGGCCACGACACGCCGGGAGGGACN (SEQ ID NO. 258) ATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCG AACATTCGGGGATGGCAGCAACCTGG :::::::::::::Rv241T7.seq::::::::::::: GGATCAACTACCGGCCAACGGTGATTCTTGGGCGCCGCTGACGCGCGAACGACCCAGCGACACATTCAGCAGATGGCC (SEQ ID NO. 259) AGCGCGTGCCGGGCCACGATGTTGGTGCTCGGCGGCTACTCCCATGGTGCGGCNCGTGATCGACATCGTCACCGCCGC ACCACTGCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACATC Clone Rv243 :::::::::::::Rv243SP6.seq::::::::::::: AGGACCGTCAGCACGGCGACGTGCTACTCGCCGAGCAGTGGGAATCGCTCTGCAGCAAACCATTACTCTGCGCGACGT (SEQ ID NO. 260) TCGAGATGACCTTCTGAATGGACGGATCTACCTGCCGCGCGACGACCTGGACCGCGTATGCGTCCGCCTCCGCCTGGA CGACACCGGGGCACTCTATGACCCCGACGGACGGCTCGCGGTACTGCTGCGGTTCACCGCCGACGCCCGCACGGTACG CGTCGGGACTGCGCTGAGTCCANCCTCGACGCCGTAGCGCTGCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCG CTCCCTTGA :::::::::::::Rv243T7.seq::::::::::::: CGACTCTGTTGGCCACTGCGGGTCGATCTTGCGGCCGCCCCGGTCGTGGAACGCCCAGGTCACCCCGCGGCGCACCGC (SEQ ID NO. 261) GGTCAGCGCGTCGTTGGCCAGCGTGGTCACATGGAAGTGGTCGACGACGAGCTTGGCGTTGGGCAGCAGCCCGGGCGT GCGGATCGCCGAGGCGTATGCAGCGGCGGGGTCGATGGCCACCGTACTGGATGCTCTCCCGGAACTGCGGTGTGCGCG CTTGCAGCCATGCCAGCACCGCCGCGCCGCCGCGGCCTTCATGCTGCCCATAAACCCTGATACCGGCCAGCTCGACNA ACCNGTATCCCACGGTCAACCC Clone Rv244 :::::::::::::Rv244SP6.seq::::::::::::: CACACGGACGGCGGTGCGGACGCAGCTGACGCGCATGGTGGTCAGCATCGCGGCCGGTCTGCTGTTGTATGCCTACTT (SEQ ID NO. 262) CGCGCCGCGCAAATGCTGGTGGGCGGCGGTGGTGGCGCTCGCATGGCTGGGCTGGGTGCTGACCCAACTCTCGAACCA CACCGGTGGGTGGGCTGGGCTATGGCCTGCCATATCGGCCTGGTGTTCTACN :::::::::::::Rv244T7.seq::::::::::::: CCGATATCCGAGCCGATAGCTGGCGGGCTCGGGTGGTNGCCAGCGGCGCTGCGACGAAAGTGTGACCGTCATGAAACA (SEQ ID NO. 263) GACACCACCGGCGGCCGTCGGCCGTCGTCACCTGCTCGAGATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGC GTGTAGTGGGTCGCCGCCCGAGCCCGGCAAACGCCGGCCCGACACAACCCCGGAACAGGAAGTCCGGTCACCGCGCC Clone Rv245 :::::::::::::Rv245SP6.seq::::::::::::: GCTTCAGGACAAATTGNATCCCTATGCACCCGTTGTCACGCCGATGAGTGAAGACTGCACGCAATCGCCGGAATCCGG (SEQ ID NO. 264) CAAAACCCTGCACAAGCGAAATCAACCGGAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGACAAACG GCAGAAGGCGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAGCGCCATCGGCATCGGTGCGGCCACGGTGG AGACGAACGTCCGCNGGCGTCTGGGTCAGTAACCCGCCGACCAGTTCTCGGGCAAGCTGGTCAACATCGGGCGCCACG TCTCCAAC :::::::::::::Rv245T7.seq::::::::::::: GTTTGGCGGCCTTATTGCACTGAGGTCGTCAATTGACCCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTCGCCT (SEQ ID NO. 265) TGGCTGCCGGAGATGGGCTCCGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTG TCGAGGATGAACTCGGCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCAGATTCGTACACATGGCCGGCGGCG ACATACCTTCACCGTGGATCTGCTCCACACGGACCGCCCTCTCGGGATCTGCTCACGGGTAAAGGAATTA Clone Rv246 :::::::::::::Rv246SP6.seq::::::::::::: GCGCACTCCTCCTTATCGCTCCGCTCTGCATCGTCGCGGCGCGGTCAGGTGCAAACGCCTTCGGGGGTGGGGCTCCTG (SEQ ID NO. 266) CGGAGCACACCGGATACGGAGCGCAACGCGTCGCGTTGTGCGGGCAAACAAGTGTGCAGGNNCCAATGCCATGTCCAG CAGCTTATCAGTGTCGAACGTGCGAACGTCGCGCCTTCGCCGGTGCCTGAATCTCTACAAG :::::::::::::Rv246T7.seq::::::::::::: CGCTGAAAGCCACCATTCGCGGGTCGGGCGCCGGGCTCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCG (SEQ ID NO. 267) CGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCTAGTGTTCGGGNCCTC TTTCGAGGTCGAGGTCGA Clone Rv247 :::::::::::::Rv247SP6.seq::::::::::::: TGTAATTTGGGATGGGCAAAAAGCAAANCACCGCGTGGCCACAAACGCGGGGAGGGACAATCTCGGGCGGCTAGGGCT (SEQ ID NO. 268) TCTCGCGGGAAGCCCGAAACGTACGGCGTTTCAACACGTCGCGTCGCCTCCGACGCGAAATTCGGG :::::::::::::Rv247T7.seq::::::::::::: CTTGGGCAACATGCTGAGGATCGCCTTTTCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTG (SEQ ID NO. 269) TTGCAGGCCGCCGGGATACCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTTGTCCCCCTGATGGCGACCTTGTCGC GTTGATCACGATGACGAAGTCACCGCCATCGACATTGGGGGCGAACTCGGCTTGTGCTTG Clone Rv249 :::::::::::::Rv249SP6.seq::::::::::::: GCATGCTTCATTATCTAATCTCCAGCCGTGGTTTAATCAGACGATCGAAAATTCATGCAGACGGTCCCAAATAGAAAG (SEQ ID NO. 270) ACATTCTCCAGGCACCAGTTGAAGAGGTTGATCAATGGTCTGTTCAAAAACAAGTTCTCATCCGGATTGAACTTTACC AACTTCATCCGTTTCATGTACAACATTTTTAGAANCATGCTTC Clone Rv24 :::::::::::::Rv24SP6.seq::::::::::::: ATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGG (SEQ ID NO. 271) GCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTCCGCTACGTCTATCCATACCGGGCG GAGCTACATCGGCTCGGCCGCCCATTGTTCNGGCCCTCTTTCGAGGTCGAGGTCTATACCGATTTGCGCATCCG :::::::::::::Rv24T7.seq::::::::::::: TCCGTACTGGTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATA (SEQ ID NO. 272) GCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATA TGCCGCTCGGGACGGTCAGAACCTCGGGTCCG Clone Rv251 :::::::::::::Rv251SP6.seq::::::::::::: GTTCTCGCACGATTTCGGATTAGCGGGATGGTCTCAATTGGGTATGCGGGGAAGGCGCTGACATTCGCCGCGATTAGC (SEQ ID NO. 273) TGTTTGATGGACCGGGGGTGATTTTTGATCACGGAAATGGGTGTTTATNCAGGTCGCACGCTTTCATCCGGGGCGGAA CG :::::::::::::Rv251T7.seq::::::::::::: GGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAAGACCCGCGGTCGAACAGCCACGTGTCCATC (SEQ ID NO. 274) ATCANGGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAACTTCATTT GCTGCGAGCAAAACAGCTGGTCGGNCGTTAGGAATGAATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGG CTG Clone Rv252 :::::::::::::Rv252T7.seq::::::::::::: ACTACCCGGCCAACGGTGATNTCTTGGCCGCCGCTGACNGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCA (SEQ ID NO. 275) GCGCGTGCCGGGCCACGANGTTGGTGCTCGGCGGCTACTCCCANGGTGCGGNCGTGATCGACATCNTCACCGCCGCAC CACTGCCCGGCCTCGGGTTCACCAGCCGTTGCCGCCCGCAGCGGACGATCACATCGCTTTTATTTNNTNTTCNGGAAT CCCTCGGGCCGCGCTGGCGGGCTGATGA Clone Rv253 :::::::::::::Rv253SP6.seq::::::::::::: ACGTCGGGANACTGTTCGCGTTCATCCTCGTCTCGGCGGATTGGTCTGCTGCGCCGGACCGACCGATCTTCAGCGGGG (SEQ ID NO. 276) GGTCACGCTCCGTGGGGTGCCGTTACTTCCGATCGCCCAGTGTGCGCGTGCTGTGGCTGATGCTGAACCTCACCGCGT TGANTTGGATCGGTTCGGGATCTGGCTGGTGGCCGGAACGCNATTTATGTCGCTACGGGCGCCGGC :::::::::::::Rv253T7.seq::::::::::::: GCTCAAAGGCACTACTGGCACCAAGGCCCACACGTCACCTGTGACTCCTGCGCCGACCCGCCCGAGGTCTGGCCGTTA (SEQ ID NO. 277) CACCGAACGGGCGAGCCGGGAGTTGGTACCATCGAACAAGACAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCG ATGACGGGTC Clone Rv254 :::::::::::::Rv254SP6.seq::::::::::::: CGATACCGGCTGCTTACCGAGACATCCACCATGCCACCCGAATCACCGCACGCGCCGAAATCGCACAACAGCTTGACG (SEQ ID NO. 278) CCTTGCAGGTTCCGCGATTGGAATTGCCGACGGTCTCTGACGGCGTCGACCTTGGCAGCCTCTACGAGCTCTCGGAAT CACTTGCCCAGCAGGGGGTTCGATGAGTGTCACACCGAAGACCTCGATATGGGCGCAATCCTGGCCGACACATCCAAC CGGGTGGTTGTGTGCTGCGGCGCCGGTGGGGTCNGCAANACACTACCGCGGCCGCGCTGGCGTTGCGCGCGGCCGAAT ATGGCCGCACTGTGGTCG :::::::::::::Rv254T7.seq::::::::::::: CGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCATCACCGATCAGCTTCGCCCCGAAGCCGCCGC (SEQ ID NO. 279) GGCGATTTCCGCTGCGACCAAACTGACCGGGGCCAAACCGGTATTGCTTACCGGCGACAACCGGGCCACCGCCGATCG GCTCGGTGTACANGTTGGCATCGACGACGTACGGGCCGGGCTACTGCCGACGACAANGTCGCAGCCGTGCNGCNGCTG CAAGCTGGAGGTGCCAGATTGACCGTGGTCGGTGACGGTATCAACGACCTCCGGCCTTAGCGGCCGCGCATGTCGCAT CGCCATGGGCAGCGCCCGAC Clone Rv255 :::::::::::::Rv255SP6.seq::::::::::::: GCACGCAATCGAAGTCACCCAAACCGGGCGGGCCAGGCGTCTNACGCCACGTCNACCAGCCGCAACCTCAACCCGGCC (SEQ ID NO. 280) ACGGCGAGCTCCTGATCAAGGCCGAGGCCATCGGTGTCTACTTCATCGACACCTACTTCCGCTCCGGGCAATATCCGC GCGAACTCCCGTTCGTCATCTGCTCCGAAGTATGCGGCACGGTGGANGCCGTCGGCCAGGGGTTAC :::::::::::::Rv255T7.seq::::::::::::: TCGACTGTGTGGCCACAGATCACGCCCCGCATGCCGAGCACGAGAAATGCGTCGAATTCGCCGCGGGCCGGCCGGCAT (SEQ ID NO. 281) GCTCGGGTTGCAGACGGGATTGTCGGTGGTGGTGCATACAATGGTGGCGCCGGCTTGTTGANTTNGGCGCGATATCGC GCGGGTGATGAGTGANAACCGGCGTGCA Clone Rv257 :::::::::::::Rv257SP6.seq::::::::::::: GAACCTGACACCCTGGTCACGGGTGAGCACGGACTTGATTTCTTCNCTATTGGTCGGCGCTGTTGAGCACACCACGCC (SEQ ID NO. 282) GCTGACGGCCGTCGCGTCCTCGCTGTGCTCGGTCTGGTGGAGCGCGCTGCCCGCGGCCNAACATCNTAAATCAAGCGT ATTCGTCAACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATACTGGTGACCTGGTCCTTCGCCAT CGATCAATGGCGATAGTCACGCAAATCGTCACGGACATCGTCGGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCAA CCCATCGGGGTGGTATCACCGCGGTGCTCGGCGATGGTCCACAATTCTTGCGGTCCAAGCCCNAAACATCCCGGGCAT GAATTCACCGGCATGCGCN :::::::::::::Rv257T7.seq::::::::::::: CTATCGTACCGCGCCGGTCACCTTCTGGATATCGCCGGCCTGGTCAAGGGGGGCGTCCGAGGGAGCCGGGCTGGGTNA (SEQ ID NO. 283) CAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCGACGACGACGTGACTCA TGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCGAGCTGATCCTGGCAGATCTGCAAACCCT GGAGCGGGCCACGGGCCGGCTGGAGAATGAAGCGCGCACCAACAAGGCGCGCAAGCCGGTCTACGAAGCGGCACTGCG TGCCCAGCANGTGCTCGACGCCGGGCAAGACGCTGTTCGCCGCGGGGGTGGATGCCGCCGCGTTGCGCGACTGAAACT GCTGACCACCAAGCCCTTCCTGT Clone Rv258 :::::::::::::Rv258SP6.seq::::::::::::: TACTCAAGCTTCAGGCCGCCACGTCCGCCGTCCGTCGGCGACGTGACCTCGAGCGCCGAGTTCGACTCGACATCGCCG (SEQ ID NO. 284) CCGGCGCATGCCGACATGAACGCGGCACTCACCGCAAGCCCGTCGGACGTCAGGTCGATCGACTCCGCTTCAAGCACC GGATCGTCCGGGCAACTCGCGGCCTCGGCCTGTGCGAACGGCACACCCGTCGTGGCGGCNCCCCGCGCGGAACTGGGC TCATCACGGTCGTTGCGAGCCGGTCGCGTCACCGCGTACCGACGCCGTC :::::::::::::Rv258T7.seq::::::::::::: CCGACATCGAGTGGGCTCGCAGTGACTTGGCGACCTCCAAGCCACCGGTACCCGCCGCGCGGCAAGCCAAGGACGACG (SEQ ID NO. 285) ACGGCCTTGCCGGATAGCTGCGCCAGGCGTTGCGCCAACTGGCGTCCAGCGTCGCCACGATCGTCAAAGAGCTTCATC TGCCGAGTGTGTCGCCATCTCATGGCTCCAAATATGGAATTAGGTCCCTGGGCCGACTGACGACAGTCCCTCAGCGAC CGGATTGCGCATCCCGCCTTGTACGCTACTCCGCAAATCCCGGGCTTGCGTCCGCGGAAGCGAACTCGGCGGCGCTAC GTGGTGGTTCACTTCGGCCGTGCGCACTCGGATCGACGGGCCGATGGTGGCCGGGCCCGCGCGCTTCTTGGTCATCCG ATTGAGT Clone Rv259 :::::::::::::Rv259SP6.seq::::::::::::: ATACTCAAGCTTGTCGCGGTAAACCGCACGCAGGGCGGTGGGTGCGGTGTCAAAGACACCCACACTTCTTTGCGGTTC (SEQ ID NO. 286) GGTGATCTCGACACCGGCCGCGAGCCGACCACCATGCGCGCGTAGATCGGCGATCAGCGCGTCGGCTATCGCCTGGGT GCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCAGCATCAGTCCGGCGCCGGCCGACACCAGTGA CGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAACGACCGCCAGGC AGGGTGCCTGGGCCATCATCCGCAGCCCGA :::::::::::::Rv259T7.seq::::::::::::: TGGACTCATAACGATCGGGTCAGCGACGCGCCAACACGAACGGCCGGACGAGTGGGCCAGGGTCGCGCCTCCCCTACA (SEQ ID NO. 287) AACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGG TCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAA TGCAATCCCCCAAAATGCAATGCGTCGAGCTATTTCTCACACCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGC GGAAGTCCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTTGGAAATCATCCTCATATGCCTCGATC GCTTCATAGGTCAAGCCCAAACCCGGCAGGATGGGTGGCC Clone Rv25 :::::::::::::Rv25SP6.seq::::::::::::: CTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATG (SEQ ID NO. 288) ACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTAGTGGTTGCGCACGTAAATTCGTCAGGT GACCGATCCCCTGCTGTCTCACTCGCCTCACAGCGACCACCACGGCTGGCGCTCAAGGCGGGCACGTGCGGAGCAGAT GAGGAATGTGCGACGTCTTGATGCAGCCTGTCAGAACACCGAGACCCTCGACGAACTTACGATCGAAACCGCTTAGGC CAACCGGTGACGGGGGTGTCTTTCCGCGGCTAGGGCGCCTTATCGTCCGAAGGCCGTGGGTGGTGATCGCCTTCTGGG TCGCGCTTGCGGGTCTGCTTGCGCCGACGGTGCCGTCCCTGGACCGATCTCCCAGCGGCATCCAGTGGCGATTCTGCC ATCGG :::::::::::::Rv25T7.seq::::::::::::: CAGGCATGCAAGCTTGCGATGTATCAACACGCCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGT (SEQ ID NO. 289) GAAATCGATGGCCAGGTCGAGGTCCCATGCGCGTGGGCCATTGATGCTGATCGCCAGGACGTCAAAGATTTGGTCCGG CGTCAGCTGGGCGAAAAACGTGGGCGCCGGGACTTGCCCGGAGCTGCCCGGGTTCCCGTCGCGCAGCTCGGCGGCCCC GGTCAGAAAGAAATTGCGCCAGGTCGCACACTCCGCGCCGTAGGCCAGCTGCTCCACGGTGTCGGCATATAGCCCGCG GGCCGCAGCGTGCTCGCTGTCGGCGAACACCGCATGGTCGAGAAGCGTTGCCGCCCAACGGAAATCACTGCGTCAAAG CTTCGCCGGGCCACTCCAGCACTCCGTC Clone Rv260 :::::::::::::Rv260SP6.seq::::::::::::: ATACTCAAGCTTGACCGACGCTGATCGCACCGCACGCGGGAACCTCAAGGGCACTACTGGCACAAGGGCCCACACGTC (SEQ ID NO. 290) AACCTGTTAACTCCTGCGCCGACCCCGGCCGAAGTCCTTGGCGTTAACACCGAACGGGCCAACCCGGGAATTTGGGTT CCATCAAAACAAATAGCAGGTGCCTGGGCGGAGTGTTC :::::::::::::Rv260T7.seq::::::::::::: GTCGTCGTGTGCTGGGGCGTCCGTATCAGCACGCCCACGAAATGGGGCACAAGAAGGATTCCTGGAACGGTGGCTGTC (SEQ ID NO. 291) CAAGATCACCCTCGCCCAAAACTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGCGGTGTCCA CACCGGGAGG Clone Rv261 :::::::::::::Rv261SP6w.seq::::::::::::: ATATGCCTTGCTGAGCTTTTCGGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTC (SEQ ID NO. 292) AAGGGGGCGTCCGAGGGAGCCGGGCTGGGTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTG GTGCGGGTGTTCGTCGACAACGACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAG ACCGAGCTGATCCTGGCAGATCTGCAAGCCCTGGAGCGGGCCACGGGGCGGCTNGAA :::::::::::::Rv261T7.seq::::::::::::: GACACCCTGGTCACGGGTGAGCAGGACTCGATTTCTTCGCTATTGGTCGGCGCTGTTGAGGCACAGCACGCCGCTGAG (SEQ ID NO. 293) GCCGTCGCGTCCTCGCTGTGCTCGGTCTGGTGGAGCGCGCTGCCCGCGGCCGAACATCGTAAATCAAGCGTATTCGTC AACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATACTGGTGACCTGGTCCTTCGCCATCGATCAA TGGCGATAGTCACGCAGATCGTCACGGACATCGTCTGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCAACCCATCG GGGTGGTATCNCCGCGGTGCTCGGCGATGGTCCAACAATTCTTGCGGTCCAAGCCCGAAACCATCCGGCCATGAGTTC ACCGGCATGGCGCAACGGCTGGTGCCGGGCAAAACGCGGCGCGATCGAATTC Clone Rv262 :::::::::::::Rv262SP6.seq::::::::::::: TGTAGAAGGTGGGTCCCGTCCAACTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATC (SEQ ID NO. 294) TATGGGTCGTGGTTACTCAACGGGCCGAAGCTGGCCCTCCCACGGGTAGGGTCCTATTCGACGGTGATGTCC :::::::::::::Rv262T7.seq::::::::::::: CCCGAATCCGGTGGCCGGCAGGGGGCCTGGCGACGTGGACACCTTCTAACTTGTCTTTACCGGTCACTGTTGCACCCC (SEQ ID NO. 295) AACACCTTTAACGACGTGGACGGACGTTACATCGGATTCGACGGTGTCATCCACAGCGTTGCCATTGGGCACACCCAC TACGCCAATTTCTCCGACTGGGACACCTACCGCAGCCTCGCCCCACTGCAGGGACTGTTGTTCCCGCAACGGGCCATC GACATGATCCAGTCGTTGGTGACCGACGCGGAGCAGACTGGTGCGTATCCGCGTTGGGCGCTGGCGAAATTCCGCCAC CGGCATGAT Clone Rv263 :::::::::::::Rv263SP6.seq::::::::::::: TTGAGATGCTGGTCGGGATGCCGATGGTTGGAACATGGTCCCCTGGCCTCGAATACGCGCGAGCGCATGAGCTCACCG (SEQ ID NO. 296) GTTCGGAACAACGTATCGAAGAACTCGCACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTC TAACTGTGCTATCGGATCTGCGTGAATA :::::::::::::Rv263T7.seq::::::::::::: CGTAATCACGATCCCGCTGAGACACTTGACCTTACGGCCGAAGTGACTTCGCTGCTGCTATGCCGACACCCGATTTCC (SEQ ID NO. 297) ATACGCTGCTGTACACGACGGCCGGGCCGGTGGCCTCCATCACGCTCAACCGCCCGGAACAGCTCAACACCATCGTCC CGCCCATGCCCGACGAGATCGAGGCCGCTATCGGGTTGGTCGAACGCGACCAGGACATCAAGGTCATCNTNCTGCGCG GTGGCGGGCGCGCCTTCTCCGGCGG Clone Rv264 :::::::::::::Rv264SP6.seq::::::::::::: CAAGCTTAAGCTGGTTCCGGCCACTCCATGAGCCGTAGTGCAATGGTTCGTGCACGGCGAGGCCGAACTTGCCATAAA (SEQ ID NO. 298) CATCCCTGACGAAAGTCTCCGGCAAGCCGATTGCTTCTTCGGGCCGCTTCTTGTGGATTGTCCGATAACCCGGTCCCT CATGCTGGAAGTTGTGCGCACTCTTTCCTTCCGCGATGTGGGCTAACGACTCGTCATTGAGCAAGAAGTACGTGCACA GGCATCGTCCGCCGGGCTTCAGCACGCGGGAGATCTCGTCCAGATAGTGCTCCACGTCCGGNGGGAAACATGTGGGTG AACACCGAGGTNAGAAACACCNCATCCAACGACGCATCCGGGATATGGAAAGCGAAA :::::::::::::Rv264T7.seq::::::::::::: TATGGTCTTCGTCGACCAGTACGTCGTAGGCGCCATGAGCCAGCGACTGAAGCCGCGCCATGCCTGCACGGCCCGCTC (SEQ ID NO. 299) ATCCAGCGAGGCGGCCATCTCCCGCAGATAGCCTGCCGCCTCGGCGCGCACGCTGTCCGGATCGCGTCCGAGCTCGTC GGCCAGCGCACGCAGCCGCTCGTCATACCATCGGGCATCCAGCAGTTGGGTAACCTCAACGGGGTCGGTCGCTAGCGG CGTCATTGATTCAGCAACAATACCGATGCGCTGCAGCAACTTTCGCAGTCCGATGCGGCCCACCTCCCGTGCAGTCAC TGGCTAGCCCCCGTCATGCCGGTTGTGTCGATGGCACGGCAGCGGGCTCGTAAACCTGCGGTCTCAGCTCGCTGG Clone Rv265 :::::::::::::Rv265SP6.seq::::::::::::: GCTTAGCGGTCTTGCTCGAACCGACATTGCGTGCCACTCATGAGCGGGTGGCGGTCGCGGTGCTTACACATCT (SEQ ID NO. 300) :::::::::::::Rv265T7.seq::::::::::::: GTATCTGGCGCCTCTCGAATATCCTTGAACGTCCCGCGGTGCCACCCAGATAGATCGCAGCGCCCTGCAATGGAGTTC (SEQ ID NO. 301) CCTTTATGGCCTCTCTAGCCTCCCGCTTGATCGGCTCGACCCGAGAGATGCCCTCGGGCGTTGCGGGATCTCCCTCCA Clone Rv266 :::::::::::::Rv266SP6.seq::::::::::::: CTTCACGCCGATCCGCGACCGCGAACGCGACGGTGACGGTGGGCGACAAGGTTCGGTTGGTCGCCGCGGCGCTGGGCG (SEQ ID NO. 302) ATATCAGCTCACCCGGTTTCGAGGTGTTCGGCGACCGGACGGTGCTGCAGACATTCTTGAGCGTCCTCGACCGGCCCG ATTCGGCCTTCAACATCGTGACGCCGTATTTCGGCGGTACCGCTCGGCGCCGAGTCGAAGGCGGCCTGAGCTAAAGCC GGGCATTGGGCGAGTGGTAAACAAGTTCGGTGACTTCGGTTGACCGACTCGACGGGCTCGATCTGGGCGCCCTGGACC GGTATCTGCGTTCGCTGGGGATCGGGCCNACCGCNANTTGCGTTGCGANCTGATTCCGGTGGAGCTCCAATCTGACTT CCGG :::::::::::::Rv266T7.seq::::::::::::: GCAGCTACCGACCCTAGCGACGAGTGTGTTCGCAGCGTCGAATGTGAACGTTCGGCGTGATTCGGCGCGCGGGTTCCC (SEQ ID NO. 303) GCTCTCAGCGCACGTTCGGCGCCGAGGNGGCTAGTCCCTGGTTAAGCAATGTCTCGGTCGCCGCCAGCAGCGCGCATG TCGCCAACCCGTCNACCGCGTTGCGCATGTCCGGTACCGACGGAAACGACGGCGCGATCCGGATGTTCTTGTCGTCCG GATCCTTTCGATACGGGAACGACCCCCCGCCTCGGTCACCGCGATACCAACGTCCTTACCCAANGCTACNGTCCGGCG CGCGGTCCCGGGCAACACGTCGAAGCTGATGAANTAACCACCCTTGGGCTCGGTCCAAGANGCGATCTTGGACTCCTT AACCGCTGATNCAA Clone Rv267 :::::::::::::Rv267IS1081N60.seq::::::::::::: TCCCCATCGGCGCCGGACCGTTTGAAAGTCCAAGCACGGGTGGGATGGAATCGACGACAGTTGAGCGCCGTCGGTGGC (SEQ ID NO. 304) CGTGGTCAGCAGCTGTTCGCGAACGCACCAGGTCACATCCCTTCGACATCTCACCGACGTGGCACGGGCGACATCAAC AGGAAGATTGACGAATCCCTCGCAGGCGCGGCACGTCCGCAGGCCAACGCCAACTACGGGGCCACCAGCGATCCTCCG CTCACGCACCAGCCCAAGCCAGGCTCANCCACCCAAGTCGGCCCGCGCTCTCCCTCGCCCCCTGGTCTCCGGGGCCTT GTTAAACAACTACCGGAAGTCCACCAATCCTCGCTGCATCTCGACACCGTCCGCCTCACTCCCTTCCTCCCGCCCCTC TCCACACNACACACCTCTTGCATTAAGGTCACGGAGCGGTCACTTTTCGTCGGACGAAATTCGCAATCCGGCCGCTCG CCGCCAGAGAT :::::::::::::Rv267SP6.seq::::::::::::: CGGAAAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGC (SEQ ID NO. 305) AGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGCAA ATCACGGTGGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTC GGCTTCAGCCCCCATCAAGGCGGCGA :::::::::::::Rv267T7.seq::::::::::::: GGCCGAGTCCAGCACTTCGCACTATGTGCAGACCAAANACCCGGTGGTCGCCGCGCTGCGGCAGCGGCTGGCAACGGC (SEQ ID NO. 306) GCCGGTGATCACCGAGTGGTGCGNAGTTGCCGACCGGCAGTTCGCCGCGGGCTTACTACGAGAAGGGCCTGCGCGACG TCATCAGGTATCACGTGTCGATGACGTCGAGCGTTAACTTCCCCGACCAGACGGCGACCTCGCCGATGGACCCCGCGT TGTACCTGGTGTGGGCGCAAGCTAACGCCGCCGCANGCTATCGGTACTCGGTCGAAGCGCAGCCGGGGTCGCAAGCGC TAGCGGGCAAGGTCGCGACGATCTCGGTCACCTGGACCAACTACGGCGCTGCTGCCGCCACCGAATAGTGNGTGCCCG GCTACCGGCTGGTGGATTCCACGGGACATGTGGTTCGGACCTGCCGGCAGCGGTGGAACTGAAGANGCTGGTCT Clone Rv268 :::::::::::::Rv268SP6.seq::::::::::::: AGCTTCAAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCC (SEQ ID NO. 307) GAGCGCCGCTGCCGGCAGGCCGATTAGGCGGGCAGATTAGCCCGCCGCGGCTCCCGGCTCCGATTACGGCGCCCCGAA TGGCGTCACCGGCTGGTAACCACGCTTGCGCGCCTGGGCGGCGGCCTGCCGGATCAGGTGGTATATGCCGACAAAGCC TGCGTGATCGGTCATCACCAACGGTGACAGCAGCCGGTTGTGCACCATCGCNAACGCCACCCCGGTCTCCGGGTCTGT CAN :::::::::::::Rv268T7.seq::::::::::::: GCTCGCGGTCCAGCAGCAGACGTGTCTGACCCCGACGCCCGGCCGCCGGTACCGAAACCGGATCGGCCCGCCGATGGC (SEQ ID NO. 308) CGCGGCCACGGCGTCTGCCTTACCCGGCCCGGATACCAGCAGCCACACCTCGCGGGAACGCTGAATCGCCGGCAGGGT CAAGGTGATTCGGCGTGGCGGCGGTTTCGCGAATCGTCCACCGCCACCACCATGCGGGTGCTCTCGAAGACGCGGGGC TGTGCGGGAACAGCGAGTTAATGTGGCCCTCGGGCCCCATGCCCAGCAGGTGGACGTCGAAATTCGGCCCGGGTCACC TGGTGCGGCACTGGCGGCC Clone Rv269 :::::::::::::Rv269SP6.seq::::::::::::: AGCTTGTCGATCGTCCGGCAGCGTCCGGCGAGTCAAGTCGAAGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAAC (SEQ ID NO. 309) TGGGCGACGGTGTCAGTGCATACCAGCGGANACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGACC GCGTTCTGGTCGTGCACCATCGAGCCGTGCCCATCCCGGCCGCGTGCCGTCAGCCGCATCCACTGGATGCCCTTCTCG GCGGTTTCAATCAGGTACAGGCGACGTTCGCCANCATCGTGCCGGGGCANGG :::::::::::::Rv269T7.seq::::::::::::: TTGGTGATCATCGNCCCAACGACCCCGAGGCGATGTTCTTGCACACCGAGGAGTGTCGCAAGCTGGGGCTGGCCTTCG (SEQ ID NO. 310) CCGCCGATCCGTCTCAGCAGCTGGCGAAGCTGTCGGGGTGAGGAAATTCGCAGGCTCGTCAACGGTGCTGCTTACTTG TTCACCAACGACTACTAATGGGATCTGCTGCTGTCCAAGACCGGCTGGTCAGANGCCGATGTGATGGCGCAGATCGAC CTGCGGGTGACCACATTGGGTCCTAAGGGTGTCCATTTGGTAGAACCTGACGCACCACCATCCACGTCGGCGTTGGTC CCCGAAACAGCCAGACCGA Clone Rv26 :::::::::::::Rv26SP6.seq::::::::::::: GGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCT (SEQ ID NO. 311) ATTTAGGTGACACTATAGAATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAG TGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGG GTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGC TGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAG TGGTGACGGCTGCCGGCATGGTGTTCGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATT :::::::::::::Rv26T7.seq::::::::::::: CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTC (SEQ ID NO. 312) AACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTA CAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGAC GGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCNCCCGGGCGCACG Clone Rv270 :::::::::::::Rv270SP6.seq::::::::::::: GGCATCTTGGCCGCCATGTTAGCCACACTGCCACCGGCTATAGAAGCGATGCGCACCGTCCTGCCAGCACATTGCGGC (SEQ ID NO. 313) GCTCCTCCCTGGAAAGCAAGATAACCAAGCTCATGCCGTGGTTGTGGGTGGCGTGGTTTGGTTTGGGTAACTTTGG :::::::::::::Rv270T7.seq::::::::::::: TCGGCTAATAATCGTCGACGCCGGCCTCCTCTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACCGGTGATCATCACGG (SEQ ID NO. 314) TGCGGATGCTCATTCGGCGCATTTCGTCGAATCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGC CGATGGCCCGCGCGCTGCTGTTATCGGTCCATTCCGCAACGACTAGGGGTGTCCCCCGCCGGAGCTGATGCCGTCGAC AATGGCACCCACCTCCTCGGTGGGGTGGGCACCGTGATCGCGAACCCACTTCATCACCGCAGCCGCGGCACCTTGCGG ATTCGACGGATG Clone Rv271 :::::::::::::Rv271SP6.seq::::::::::::: CTCAAGCTTGGAGGCGTGGCGATCGCGGTCCAAGGCGCGCTCTCCGAGCACAACGAGCGAAGACNGCTCGGCGACGGA (SEQ ID NO. 315) GCCTTTATCGACNTCCGTTCGGGCTGGCTGACGGCGGCNAAATAATGCTGGACTCGTTGTTGTCGACGGTGCCGTGGC GAGCCGAGCGCCGTCAGATGTACGACCGGGTGGTCTATGTGCCGCGGTTGGTGAGTTTCCACGACCTGACCATCGAAG ATCCGCCGCATCCGCTGCTGGCGCGGATGCGCCGGTGGCTCAACTAATTCTACGGCGGCGAACTGGGTNATCCCTTCN CCACCGTCGG :::::::::::::Rv271T7.seq::::::::::::: CCTAGGTCAACCGTACCGTCATCGGATCGGGGTCGACCGCACAGATGGACTGGAGCTTCGGCGAGGTCATCGCCTATG (SEQ ID NO. 316) CCTCGCGGGGGGTGACGCTGACCCCGGGTGACGTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCGAAGCAC CTCAGGCCACCGGAAATCATTCCCGGGCTGGCTGCACGACTGCGACGTGGTCACCCTCCAGGTCGAAGGGCTGGGCGA GACGATGCAGACCGTCCGGACGAGCGGCACTCCTTTTCCGTTGGCTCTTCGGCCGAATCCGGACGCCGAACCCGACCG GCGCGGGGTCAACCCGGCACCGACGCGGGTGCCGTTTACCCGCGGGCTGCACAAATCCCGACGGGTATGGGCTTTGAC CTGCCGACGGGGGA Clone Rv272 :::::::::::::Rv272SP6.seq::::::::::::: AGCTTGGCGTGACACCAACACAGGGCACTTAAGATGGCAATGCGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGA (SEQ ID NO. 317) TGCCGAGGGGAGAACAATGCGAGCACGGCCGCTGACGTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGC GGGCTGCGAGGCCCGAGTCTAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGTCTCGACCGCAAGCGCGACCTCA GCCGCAGCCGGTGGAGCTACTGCTGCGCGCCATCACGCCGCCTAGGGCTCCGGCGGCGTCGCCGAACGTCGGGTTTGG CGAACTGCCTACCCGGGTCCGGCAGGCAACCGAT :::::::::::::Rv272T7.seq::::::::::::: TCATGCCGTTGGACCGACCATCGGAGTTAGTTGCCGAACCGCGGGACCACCGCAAGCACCCGGTCCTGGTCGCGCACC (SEQ ID NO. 318) GCGTCGGCCAACCGCTTGAGCACCACCACGCCGCAGCCCTCGCCGCGCACGAATCCATCCGCGTTGGCGTCGAAGCTG TTGCATCGGCCGGTCGGTGACAGCGCCGACCACTTGGACAGCGCGATGGCGGTGAACGGTGACAAGGTGAGCTGCACC CCGCCCGCCAATGCGACGTCGGTTTCACGCAGGCGAAGCTCTGACACGCGAAGTGAATTGCCACCAGCGACGACGAAC AAGCGGTATCTACGGCGATGG Clone Rv273 :::::::::::::Rv273SP6.seq::::::::::::: GGGTCGACTTTCTGCAAGGCGAGGCTACACCGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCAT (SEQ ID NO. 319) CACCGATCAGCTTCGCCCCGAAGCCGCCGTGGTGATTTCCGCTGCGACCAAACTGAACGGGGCCAAACCGGTATTGCT TACCGGCGACAACCGGGCCACCGCCGATCGGCTCGGTGTTCAGGTTGGCAT :::::::::::::Rv273T7.seq::::::::::::: AATCCGAAATCCTGACCGATACTTGAACCTGGTCTCGTTCGGCAATAACTCGTCGGCGTGCAGGACCCGGCGCAAACG (SEQ ID NO. 320) TACTTCGGCATCAACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCTAACAGCACG CTCTTCCCGTACACCAACCCCGACGGCGCGCTGGCCCGGGCGGAACGTGGTCCTCGACACCATGATCGAAAAACCTTC CCGGGGAGGCGGATGC Clone Rv274 :::::::::::::Rv274SP6.seq::::::::::::: TTCCGAATTTCGGGTCCNGGTCATATGACCCTCATGGAAGAAGAAGCGGCCGCCCCGCGCCCGTGCGACGGCGAATGA (SEQ ID NO. 321) AAACCCTCACCCAGGCCGCATTGAACGCCGACAAGACGGTGGAGCAGGTCGAAGACGTCCTGGACGGTCTGGGTAAGA CCATGGCCGAGCTGAACAGCTCGCTGTCACAGCTGAACAGCACCGTGGAGCGCTTGGAGGACGGTCTGGACCATCTCG AAGGTACCCTGCACAGCCTGGACGATCTCGCGAAACGGCTCATCGTGTTGGTCGAGCCGGTGGAAGCCATCGTCGATC GGATCGACTACATCGTGAGCCTCGGCGAAACGGTGATGTCACCGCTGTCGGTC :::::::::::::Rv274T7.seq::::::::::::: NCTCGATCTTGGGGTACGTTCGATGAGGCTGCTGACCAACAACCCGGCCAAGCGGGTGGGACTGGATGGATACGGATT (SEQ ID NO. 322) GCACATCATCGAGCGCGTGCCGCTGCCGGTGCGGGCCAACGCGGAGAAACATCCGTTACCTGATGACCAAGCGTGACA AATTGGGGCACGACTTGGCTGGGTTGGACGATTTTCACGAATCCGTGCATCTGCCCGGAGAATTCGGCGGTGCCTTGT GAAGGTGGCGCCGGGGTGCCGGATCTGCCGTCGCTGGATCGTCTGGTGTGCGGCTGGCGATTGTCGCCAGCAGCTGGC ACGGAAAGATCTGCGACGCGCTGTTGGACGGCGCCCGCAAGTGGCCGCCGGGTGTGGCCTCGATGACCGACTGTGGTT CGGGTGCTCCGCGCGATCGATAT Clone Rv275 :::::::::::::Rv275SP6.seq::::::::::::: TCATCCCGACCAAAACGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCGCTGCCGGCAG (SEQ ID NO. 323) GCCGATTAGGCGGGCATATTATCCCGCCGCGGCTCCCGGCTCCGAGTACGGCGCCCCGAATGGCGTCACCGGCTGGTA ACCGCTCTTGCGCGCCTGGGCGGCGGCCTGCCGGATCAGGTGGTAGATGCCNACAAAGCCTGCGTGATCGGTCATCAC CAACGGTGACAGCAGCCGGTTGTGCACCAAGCGCGAACGCCACCCCGGTCTCCGGGTCTGTCCAACCGATCGACCGCC CAAGCCCACATGAACAAACCCCGGCATCACGTTGCCGATCGGCATACCGTGA :::::::::::::Rv275T7.seq::::::::::::: TTGGCGGGTTGGCCCAGAGCCCGCCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGCCACCGCGGCTTGC (SEQ ID NO. 324) ATGCTGGTTGGCTGTCTTGGGACGATCCCGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCCGCGATTACC CCGCGCGGCTCGACGAGTTTTTGGCCTGGACTACCCGCGTGGCCAATCTGCTGAACTCGCGGCCGGTGGTGGCCTGGA ATGTCGAGCGCCGTTACCTACGTGACCTGATGGATCGGGGGGTGCCGACCGTGCCCGGCGATGTGTATGTGCCGGGAN AGCCGGTCCGGTTGCCACGCAAAGGCCATGTCTTCGTCGGTCCGACCATCGGTACCGGGACACGGCGCTGTATTGCCC GGTTCGCTGCCGAGTTCGTCGCGCAACTGCACGCNGGCGGGCCAGCGGTGCTCGTTCANCCCGGAGGTTCCGGTGACG ATGATCGTGTTGGTCTCCCT Clone Rv276 :::::::::::::Rv276SP6.seq::::::::::::: GTAGGAGAGAACAAAGACCGTCGATAGGACACGTGTTACGCCGGTAGCTGTCATTGGTATGGGGTGCCGCTGCCGGGG (SEQ ID NO. 325) GGCATCTACTCACCCGATCGGTTGTGGGAGGCGTTGCTGCGGGGCGACAATCTGGTCACCGAGATCCCCGCCGACCGC TGGGACATCTACGAGTACTACGACCCCGAACCCGGCGTGCCCGGACGCACCGACTGCAAATGGGGCGCGTACCTCGAT AACGTCGGCGACTTTGATCCCGAGTTCTTCGGGATCGGGGAGAAAGAAACGATAGCGATCGATCCGCAGCACCGCTTG TTGCTGGAAACCTCCTGGGAAGCCATGGAACACGGCGGGCTAACACCGAACCATATGCCTCCCGACANGGGTTTTCGT GGGGTT :::::::::::::Rv276T7.seq::::::::::::: CGAACTGAGCCCATAGAAAGGCAGCGACTAATTCGCTGGGCAAATAGGAAGACCCTTTGTCCTGCCACGTATATTTGT (SEQ ID NO. 326) CGACCTCGTTGCGAAGGAAGCGGCTGCGATTGGTGCCCTTTTCCCTGGAGAATCTCTGCCCGGAGCAGGAAGTCTTAT GAGTTGACAAGCAGGGGCGCCGCCTTCGCCGGAAATCACATTCTTGGTCTCGTGAAATGAGAGCGCTCCCAGGTCGCC GATGCTGCCGAGCGCCCGCCCACGATACGACGCCATCGCGCCTTGGGCCGCGTCTTCGACCACCGCCAGGTTGTGGTG CGTGGCGATCTTCATGATCGCGTCCATCTCGCAGGCCACCCGGCATAGTGAACGGGGACCATGGCCTCGGTTCGCGGG TGAA Clone Rv277 :::::::::::::Rv277SP6.seq::::::::::::: CTTAGACGCCACCTCCGGGCCGAGCTCCACGGGGTGGATAAGTACGGCCGGATGTGGCCGCAATGGGAAGTTGTTGCC (SEQ ID NO. 327) CGCTTGACTGTCCGGGTTAACGCCGGATTCCACCACATCCCCTTGCGAAAGGCCGTTGGGTT :::::::::::::Rv277T7.seq::::::::::::: GATCGCGATCGTCGATGTGGCCATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCAGATGGTTCGCGGCACGGTCAA (SEQ ID NO. 328) CCTGCCACACGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCG GGGGCTGATGCTGTCGGATCGACGATCTGATCGAGAGGATCAGGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGAT ACCGGATT Clone Rv278 :::::::::::::Rv278SP6.seq::::::::::::: AGCTTACGCCGCTTTCGCTTCNGATTTGGGACGCCGCATCGAAAGCGCAGTTGGAAGCGCGGCGCCCGGCTGGTCGAG (SEQ ID NO. 329) CTGCTCAAGCAGCCGCAATCCCAGCCCATGCCCGTTGAGGAGCAAGTGGTTTCGATCTTCCTGGGCACCGGCGGTCAC CTGGACTCGGTGCCCGTCAAGGATCTCGGCGGTTCGAAACCGAATTACTGGACCACATGCGGGC :::::::::::::Rv278T7.seq::::::::::::: CGACGGGACCTCGTCGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTATAAGGTCGGC (SEQ ID NO. 330) GAAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGATGCCCTCGGGTCCNGCCAGCACTCCTCAGG CTTCGTCGGGGTGGTCGCGACCGCATGGGCCACATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTANACGGTT CCTTTCCTAAGCAACACCGAAATTTCAGGACCCGAATGCTCCGGGAAAACATGTCACGGTAAGTCCGGTATTCCGGGT ACCGGTTGAGCATTGA Clone Rv279 :::::::::::::Rv279SP6.seq::::::::::::: CGGCATCGGTTTGGGCTGTCACCAGCAGTTGGTAGTTCTTCACTACTGTTGTTCGAGCGTCGAGCCGCCGCGCGTGTC (SEQ ID NO. 331) GAGGTCGCCGGACGCGTACCCGCCAGGCCGGTCAGGGTGCCCTTCCAGTCCACGCNGCTGTGGTCGGCTAACCGCTTA TCTTCAATCGAGACNATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCACCTCGAACCGGCGCTGCGANTAC AGCCACGCGATCGTGTTGCCCTTCGCGTCGACCATCGTCGATACCGCAGGCACTTGCCCCTCGAGCAGCTGGGCCGAT CCGTTGGCAACGACCTCAGAGGCACGATTGGACATCAGCCCTAGCCCGCCTGCG :::::::::::::Rv279T7.seq::::::::::::: CCGTCGANGCCGCCGACTTGGCTTGACCGACACCAACATGGCCTGAGGGTGTTCAACAAGACCGTGGCCGACGGGCTG (SEQ ID NO. 332) AACATCACCATGAGCGGCATGAGCCACGCCACCGAGTTCATCATGTTGATCGCCGAAAACCATTGGCGGGTAGCGGAA GAACGGTCGAGGTGCTCTACACCGAGTATTCGAAGTCGAAAGGCCAACCGCTGCTCAACGGCGTCAACATCATTTTCG ACGGGTTTCTGCGAGGGAGGATGCCACGATGAACTGGATCCAGGTGCTGTTGATCGCGTCGATCATCGGGTTGCTGTT CTACCTGTTGCGGTCGCGCCGAAGCGCGCGGTCCGTGCCTGGGTCAAGGTGGGCTATGTCTTGTTCGTGCTCCCGGCA TCTATGCCGTGCTGAGA Clone Rv27 :::::::::::::Rv27SP6.seq::::::::::::: TTACACGNCCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGAC (SEQ ID NO. 333) CATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGG CAATTCTACTAGCGAGAAGTCTGGCCCGATNCGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGAT GGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGNGACGCGGTANGTGGTCAAGTCCGGTCTACN CTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTGCGCCGCGGAAAGCGGCGGGTCGGGTGCCATCAGGAATG CCTCACCGCCGCGGCACTGNACGGCCAGTGCCGCGGCGATGTCNGCCATCGGGACATCATGCTCGCCTTCATACTCCT CGACC :::::::::::::Rv27T7.seq::::::::::::: CAGGCATGCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCA (SEQ ID NO. 334) GGTCGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTG GCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATT GGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGG Clone Rv280 :::::::::::::Rv280SP6.seq::::::::::::: AGCTTAGCCAGTTTTTCTACTCTTGGGCCCACACCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGT (SEQ ID NO. 335) TGGCATCGGTGAGCTGATAAATGCCAGCTGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGA TACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACTTTTCGGATCTCTTCGGCGACTTCGATGGGGTCGTCGG GAGTCCCGGGCGCCACCGCGAGGTAAGCCTCGTCCCAGCCCCATACCTCGACCGGGTATCCCAGGTCGCGCAATAACG CCACCACCTCCTCGGACGCCGCGTTGTAGGCGGCTGGGTTCGACGGCAAGAAGTGGCCTCAGGGCATCGTCGGCGCGG TCCCAACGGCNTGCCGGCGCGCACACCGTAGGCGCGGGGCTC :::::::::::::Rv280T7.seq::::::::::::: CCGGCGGAACTCAGACGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCCGGGGACGACAGCAA (SEQ ID NO. 336) GCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATGT TTATGCCGCCGACCGGGTGCGCTGCCACCAGACGATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACAACGA GCCCACCCTGACCGAAGACTCCTACGCCAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCGTCGAGCAAGT AGGCACACCCGTGATCTGCACGCAGGGCAAGGTCATTCCCGATCTGATCACCTGGTGGTGCGAGCGCGACCCTGTGCC CCCGACAGTCCCGCAATCGCAAAGGCAGCACGTTGGTGT Clone Rv281 :::::::::::::Rv281SP6.seq::::::::::::: GTATGGTCAGCTGTCCATCCGGCGCTGTCGGCCGAGCTGCCAGATCTCGTCAGCCGTAACCGGGTTGCGGGATCCACG (SEQ ID NO. 337) CGTGCGGGTTGTCTAC :::::::::::::Rv281T7.seq::::::::::::: CCGACTTTCCGCGGGTACCCGCTCAACTTTGTGTCNACCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACT (SEQ ID NO. 338) ACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACT ACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCCGTGCCGATCGTGGGGAACCCACTGGCGAACC TGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAA TGTTGCGACTCCGTTCGGTTGTTCCAGAANGTCAGCCCG Clone Rv282 :::::::::::::Rv282SP6.seq::::::::::::: GCACCGATGTCGGCGAGCACTTCGTCAACTTCCAGGGGTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCC (SEQ ID NO. 339) GCCGCCGCCGGTGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGGACTCGCGCGCGTACCGGCTGCCTCGGC :::::::::::::Rv282T7.seq::::::::::::: TGCACCCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGGG (SEQ ID NO. 340) CGTAGCTGGGAGAGGCCCCGGTCAAGCCCGGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGC GCTTCTCGCCGTATTGAACTGGCGTGCTGACGGTCGCTGAGCAGCGCTCGCCGAGTGCGGCCGCTGATTCTTTCATCG AGCCAGGACGCGCATTCGTGTTCGGCCGC Clone Rv283 :::::::::::::Rv283SP6.seq::::::::::::: AGCTTACGGCCGGTCGACGCGACGAGTGGTTCATGACACCACAAACCGTCAACGCCTACTACAACCCGGGGATGAACG (SEQ ID NO. 341) AAATCGTCTTCCCGCAGCGATTTTACAGCCACCATTTTTCGATCCGCAGGCCGACGAGGCCGCCAACTACGGCGGGAT CGGGGCGCGTGATCGGGCACGATGATCGGGCACGGTTTCGACGATAGGGCGCCAAATACGANGGCGACGCAATCTGGT CNATTGGTGGATCGA :::::::::::::Rv283T7.seq::::::::::::: ATGTCGTCACGTCACCACAATCGCGAGGACCCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAAGCGG (SEQ ID NO. 342) CAGCTCGATCGCAGCGCGGAGGTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTG ACGAACGCAAGACCTATATCTGCCGTCGGAGTAAGAATCGAGTAGCCGGTCGACCAACGGAAGCGAAAGTCTCCGCGA TGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGC Clone Rv284 :::::::::::::Rv284SP6.seq::::::::::::: AGCTTCACCAGCGTGCCGATGCTGTTCGCNACACCTCCCTACTATGCGCAATTCGCCGACACGGGTGGCATCAACACG (SEQ ID NO. 343) GGCGATAAGGTGGACATCGCTGGGGTGAACGTCGGGCTGGTGCGCTCGCTGGCAATCCGCGGCAACCGCGTGTTGATC GGATTCTCGTTGCCCGGCAAGACAATCGGGATGCAAAGCCGGGCAGCAATTCGCACCGACACCATTCTTGGCCGTAAG AACCTGGAAATCGAACCCCGCGGTTCGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCAGAACACTACGCCA TACCAAATCTATGACGCGTTCGTC :::::::::::::Rv284T7.seq::::::::::::: CTGCCGCGGTGGCGGTCAGCGCCTGGCAAGTCACCGCACCGCCGTCCGGTTCATCGGCAGGCTCCCCCGAAAAGGGCC (SEQ ID NO. 344) CTGGCAACAGAAGGTGATCAATGAGCTCCCGCAGACCTTCGCCGATCTGGGACCGACATACGTGAAGTTCGGCCAGAT CATCGCGTCCAGCCCGGGAGCATTCGGTGAGTCGCTGTCGCGGGGAATTCCGCGGCCTGCTCGACCGGGTGCCGCCCG CAAAAACCGACGAGGTGCACAAGCTCTTCGTCGAGGAACTCGGCGACGAGCCGGCCCGGCTGTTCGCCTCCTTCGAGG AAGAACCGTTCGCGTCTGCGTCCATCGCCCAAGTGCACTACGCGACCTGCGCAGCGGCGAAGAAGTGTGGTCAAGATC CACGGCCGGGCATCCGCCGCCGCGTTT Clone Rv285 :::::::::::::Rv285SP6.seq::::::::::::: GATCGTGCCGGCCCCCCGGCGGCAGTAGCAGATCAGCTCGTCGAAATCGCGGCAACCAGTCCAGTCGATTTCCATACG (SEQ ID NO. 345) GGCGCCGTCAATCAACTCTGCGAACATCGCGATCGGCACCGGAAACCGGCGAGCCGCGTCAGCCAGCGCAACCAGCAC CGGGATCGGATGAATCATCAATATTATCAAGTGATTTCCTGATGGCATCGAGCTCGGTGATCTTGGTCTCGGGGGCCA GCTCGCCGTCGGCGACGTCGTCGATCCGGCGGCCGAGCGCATAGACCGCAAATAGTGCCGCTCGCTTTTCGCGCGGCA AGAGTCGGATGCCGTAATATANGTTTCTGGCGGCCGTGCGCGTGATCNACTCGGTGATTCGATACGCCTGTTCATCTC GGTCATGCCGTCCTC :::::::::::::Rv285T7.seq::::::::::::: GGTGGCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCCGGGCCCGCACAATCGTTCGTGTTG (SEQ ID NO. 346) GAGCGGCCCATCCAGACCGTTGGGCGCCGTAAGGAGGCCGTGGTACGAGTGCGGCTGGTGCCCGGCACCGGCAAGTTC GACCTCAACGGCCGCAGCTTGGAGGACTACTTCCCAAACAAGGTGCACCAGCAGTTGATCAAGGCACCCCTGGTCACC GTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCCGTCGGGTCATGGCCGGCGCGCTGCG CCTGGGTATCGCCCGGGCATTGATTCTNGTATCGCCGGATGACCGGCCCGCGCTGAATAANGCCGGCTTCTTGACCGT GATCCACGCGCCACCGAACGCAAA Clone Rv286 :::::::::::::Rv286SP6.seq::::::::::::: CACAATAGATTACTCAAGCTTCGAACCAGCGGCCTTATCACGTATCCCCGCTGAGACCTTGACCCTTAGGGCCGAAGT (SEQ ID NO. 347) GACTTCGCTGCTGCTATGCCGACACCCGATTTCCAGACGCTGCTGTTACACGACGGCCGGGCCGGTGGCCACCATCAC GCTCAACCGCCCGGAACAGCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCGAGGCCGCTATCGGGTTGGCCGA GCGCGACCAGGACATCAAGGTCATCCTGCTGCGCGGTGCCGGCCGCGCCTTCTCCGGCGGTTACAACTTCGGCGGCGG GTTCCAACATTGGGGGCAT :::::::::::::Rv286T7.seq::::::::::::: TCAGGACGCTTATGGTTGGCAGATGGTCGCCCTGGCGTCGAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAA (SEQ ID NO. 348) CGTATCGAAGAACGTCGCACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCT ATCGGATCAGCGTGAATATCGAGATATTGCGAATGCGATGACAGGCCGCCATTCGGTTTATTCGCTTACGCTTCCCGG GTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAATTATTGATGTGGT AGGCGGCAGCTGCCGTTTTGTGCTGTCGGGCTATTCATCGGGTGGGGGTGTTTGGCTATGCCCTCTGCTCCCAT Clone Rv287 :::::::::::::Rv287SP6.seq::::::::::::: CGCAGCTGTCGCCGATCTGGTCCGGAATACCTAGCTCCAGGTTCTGAGTGGAGATGAGTGCGGCCATCGAAGTGTTGT (SEQ ID NO. 349) CAATGTACTCCAGGATGTCAGGTGCCAGGCCGCTGGCGAGGATCTTGGGCACCGCCGCCATGACTTGGTCGAAGTCGG CGAACGGGGCGAGCACGCTGGCGTCGTGGTC :::::::::::::Rv287T7.seq::::::::::::: GTAGTTCGTTCATCCAAACACAGTGCGGTACCGGCTCAAGCGGATCACCGACTTCACCGGGCGCGATCCCACCCACCC (SEQ ID NO. 350) ACGCGATGCCTATGTCCTTCGGGTGGCGGCCACCGTGGGTCAACTCAACTATCCGACGCCGCACTGAAGCATCGACAG CAATGCCGTGTCATAGATTCCCTCGCCGGTCAGAGGGGGTCCAGCAGGGGCCCCGGAAAAGATACCAGGGGCGCCGTC GGACCGA Clone Rv288 :::::::::::::Rv288SP6.seq::::::::::::: TCCGCTCGCTTCTCCGAGAGGTTGAGTGCCAACGCTCTGCCGATGCCCGAAGCCGGCCCCGGTGATGACGGCGACCTT (SEQ ID NO. 351) GCCTTCGAATGAGCTCATTTGACTACTCCCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCCCG AGGTCGGCGATCGCGTCTCGGGCTTCGGGGAGCAGACTGACCTGCAGATGGAAGTCGTGCCACATGCCCGCGAACCGG CGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTTCGATCTTGTCCGCGTCAACACNGATCGGATCGTCGCCC GCGGTCTGCATGACGAATGGGCG :::::::::::::Rv288T7.seq::::::::::::: ATGGGAGGCCACCGATTACCATCTTGCACACACCGATTCCGGGCTATTGATGTCCACGTTCGGTCCGCGAACCGCGCT (SEQ ID NO. 352) GTGGCTGCTGCTGGCCAAAGGCGGAGGCGATACCGAAGTCAGTGCCCAAGCTTGGGTTCCACGCTCGCGCAGCCACGC CGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAATTGGCGCAGCGAACACTCAA CGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTCCGCACGGCGACGTTCTACACCCGCACCAA GATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCACGTTCTTGAACCTATTCG AGCTGGAATCGGCCGTCCGGTTGCTGGGAATTGCNGTTAAGAACTGGGCCT Clone Rv289 :::::::::::::Rv289SP6.seq::::::::::::: GCTTTGCGCGCTTCTCCGAGAGGTTGGAGTGCCAACGCTCTGCCGATGCCCGAGCCGGCCCCGGTGATGACGGCGACC (SEQ ID NO. 353) TTGCCTTCGAATGAGCTCATTTGACTACTCCCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCC CGAGGTCGGCGATCGCGTCGCGGGCTTCGGGGAGCAAACTGACCTGCAGATGGAAGTCGTGCCACATGCCCGCGAACC GGCGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTCGATCTTGTCCGCGTCAACGCAGATCGGATCGTCGCC CGCGGGTCTGCATGAAGAAT :::::::::::::Rv289T7.seq::::::::::::: CTCACGCAGCCACGCCGTCACCTTTCCACGAAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAAATTG (SEQ ID NO. 354) GCGCAGCGAAACACTCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTGCGCACGGCGAC GTTCTACACCCGCACCAACATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCA CGTTCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAAACTGGCCTAGAAACCGGCGG GCACACCGCACCTGGGCGGGGN Clone Rv28 :::::::::::::Rv28SP6.seq::::::::::::: TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACG (SEQ ID NO. 355) CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACC ACNCGCGGGTCGGGCGCCGGGCCCGGGTCGCCANGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGC TGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAAGTCGAAG TCGATACCGATTGCGCATCCGCNGCCGCA :::::::::::::Rv28T7.seq::::::::::::: CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGAC (SEQ ID NO. 356) CTCGTCTGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTC GGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAACCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGG GTGGTCGCGACGCGCATGGGCCACC Clone Rv290 :::::::::::::Rv290SP6.seq::::::::::::: GCTTGTCTATCGTCCCGGCCAGGTCCGGCCAGTCAAGGTCGAACGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGA (SEQ ID NO. 357) ACTGGGCGACGGTGTCAGTGCAGACCAGCGGAAACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGA CCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCCAGCCCGGCCGCGTGCCGTCAGCCGCATCCACTGGATGCCCTTCT CGGCGGTTTCAATCAGGTACAGGCGACGTTCGCCACCATCGTGCCGGGGCACGGTTAGCGAGAAACCGCCGACTTCAC GATTGCCTCGGTGATGCCGTCGAAACAGATCGGGCCT :::::::::::::Rv290T7.seq::::::::::::: GCGCGCCATGTTGAGGTTGTCCGACGGTGACGACGGTGAACCACAACTGTTTGACCTGTCCGCACACACCGTGTGGAT (SEQ ID NO. 358) CGGCGAGCGGACCCGACAAATCGATGGCGCGCACATCGCGTTTGCCCAGGTGATTGCTAATCCGGTCGGGGTCAAGTT GGGCCCCAACATGACCCCGGAACTGGCCGTGGAGTACGTCGAGCGGCTCGACCCGCACAATAAGCCGGGCCCGCTGAC TTGGTGAGCAGGATGGGCAACCACAAGGTCCGCGATCTGTTGCCACCGATCGTGGAGAACGTCCATGCCACCGGGCAT CAGGTCATCTGGC Clone Rv291 :::::::::::::Rv291SP6.seq::::::::::::: TTGCCTTCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGGTGTTGCTGCTGGTTCCGGGC (SEQ ID NO. 359) TATGAGAGCACTGCTCATATGATTAGCACATTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACACTCCTTGCG CAGCAACCAGACCTGATCCCGCCGGCGATCGAGGA :::::::::::::Rv291T7.seq::::::::::::: CGACGCTGGGCCCAACTGCGACCACCAGGTCCTGGTATGGCAGGACATGGCCGGGTTCAGCGGCGCCAATACCG (SEQ ID NO. 360) Clone Rv292 :::::::::::::Rv292SP6.seq::::::::::::: TAACGACTCGGGTCCAGCGACCCCGCCAACACNAACGGCCGGACNACGTGGGCCAGGGTCGCGGCCTCCCCTACAAAC (SEQ ID NO. 361) AGGATCCGTTGCCTGCGAACGACAGGCTCCGGTCCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCG CCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAATGC AATCCCCCAAAATGCAATGCGTCNAGCTATTTCTCACACCGACCGCTAGTTGCGGATCANAAATCCGTTGGGCGCGGA :::::::::::::Rv292T7.seq::::::::::::: CNTGGCGGTGGGTGCGGTGTCGAACACGACCACACTTCTTTGCGGTTCGGTGATCTCGACACCGGCCGCGAGCCGACC (SEQ ID NO. 362) ACCATGCGCGCGTAGATCGGCGATCAGCGCGTCGGCTATCGCCTGGGTGCCGCCCACCGGAATCGGCCAGCCGACCGA ATGGGCCAGCGTTGCCATCATCAGTCCGGCGCCGGCCGACACCAGTGACGGCAACGGTGAAATCNCGTGGGCGGCAAC GCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAGCGACCGCCAGGCAGGGGTGCCCTGGGCCAGCATCCGCAGCCC GAGACNCAGGACCGANCCCAGTG Clone Rv293 :::::::::::::Rv293SP6.seq::::::::::::: GCTTTTCNGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGA (SEQ ID NO. 363) GGGAGCCGGCCTGGGTAACAAGTTCCTGGCTCATATCCGCGAATGCNACGCCATTTGTCAGGTGGTGCGGGTGTTCGT CAACAACNACTTGACTCATGTCACCGGACGGGTCGATCCCCANTCCGACATTGAGGTCGTCGANACCGAGCTGATCCT GGCANATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAAGGAANCGCGCACCAACAAGGCGCGCAAGCCGGT CTACGACGCGGCACTGCGTGCCCAGCAGGTGCTCGACGCCGGCAANACGCTGTTCGCCGCGGGGGTGGATGCCG :::::::::::::Rv293T7.seq::::::::::::: GTCGTACGCCATTNGTCGGTGTGCGCATACCAGTACGACGCGCCGGGCACCTGACGCGGCGGCCGCCACCAGTCGGTG (SEQ ID NO. 364) GCCATCGCCATCGTCTGCCACCCGGTCAACGGACGCACCTTCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTG CGTCCCATCNATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATCGCTGTGGAACCAGTGACAGATT CCGCCGCCCATGATGATCATCGACCGTCCTCCGGATTCGGTCGCGTTGCGGGCGAAATTCCTTGGCAAACCGGATTGC CTGCGCGGCCGGCACACCGGTGATCGACTCCTGCCAGGCCGGGGTGTTCTGCTGGGTTCGGTCGTGGTACCGGT Clone Rv294 :::::::::::::Rv294SP6.seq::::::::::::: GCGAGGCGGTATCGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTCCTGGGGATTCTC (SEQ ID NO. 365) ACGCTGCTGCTGANTGCGTGCCANACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTG AANTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACNACTCCAAGCTGGCGCCGTCTCGTCCG CAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGG ATCGAATACNAATTGATCACCCA :::::::::::::Rv294T7.seq::::::::::::: TGGGTCTTGCCGGCGAGCCCAGCGAAGTCGCTAGCGTGGCCGTGTTTCTTGGCTTCGGATCTATCCTCGTTACATGAC (SEQ ID NO. 366) CGGCACCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGA AGGAAATCTTTCCCNATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGAAATCGTCGATCGGCTGGGTGTTA TTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCGGGCGTGCCGGCGCATTCGAGCTG GCGGTGCGCGCTGCCCAGCACCGTCATAAGTACTTGANGATGGTCAAACGTCGGACGAACCGCCACCACGTCGCTGCC GAACGG Clone Rv295 :::::::::::::Rv295SP6.seq::::::::::::: TAGATGCCCAAGCTTGCCNTTANAGACCTCGTCGACCAAGCACGGACGCGACCGTCGAAGGTGGCGAATCCGGGCTTG (SEQ ID NO. 367) GCGTCNACCCGCGTAAGGCAGACCAGATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCG TCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCGGATGTTGTCGGGAGTGACGATCTGA TCGAGAGGATTCAGGGCGGCTGGCTGGA :::::::::::::Rv295T7.seq::::::::::::: TCTCCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCN3GTTGTTGCCCGGCTTGATGTCTGCGTTAG (SEQ ID NO. 368) CGCCGGATTCCACCACATCCCCTTGCGAAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGG AGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATTG CGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTNGGTAATCCGGCC Clone Rv296 :::::::::::::Rv296SP6.seq::::::::::::: GCCCGGTTCGATCGGGCATGTCCGCAGTCGTCGTTACCGGAGGCGGTCGTGGCCGCGCTAATCGGCGTCGGCGCCGAC (SEQ ID NO. 369) AAGATGTGGGATATCCGCAATCGGGGCGTCATCCCTGCGGGCGCGCTCCCCCGCGTCCGAGCCTTCGTCGACGCAATC GAGGCAAGTCACGACGCGGATGAGGGGCAGCAGTGAATTACAGCGAGGTCGAGCTGTTGAGTCGCGCTCATCAACTGT TCGCCGGAAACAGTCGGCGACCGGGGTTGGATGCGGGCACCACACCCTACGGGGGATCTGCTGTCTCGGGCTGCCGAC CTGAATGTNGGTGCGGGCANCGCCGGTATCNACTCCCGTGGAACACAGCCGGGGC :::::::::::::Rv296T7.seq::::::::::::: CTCGGCGTGGATATCGGTGTAGCCGGCGCCGGTGAANGTCGGCTCCTTACGTCCACTCGACAACAGCTCATAGCGATC (SEQ ID NO. 370) CAACCAGTANGCAACCGCCTTCAGCAGTACAACCGCGCCGGCGAACACTGCGAGTTGAACGCGAGCTGCCTGGGTCAG CATGCCTCTGCCGGTTGTCAGCCGAAGGCCGCCGAACAGGTAATGCGTCAACAGGCTCGCTAGAAACGCCAGAACCAC GGCCACGAACAGCCAGTTCAGCACCGACCGGTAGAACGGCAGATCGAAGACGAAAAAACCCAATGTCATAGCCGAATT CGGGGTCCACGATGCCAAAGGTGCCCCCGTGTACAACAACTGAACCTTGACCCA Clone Rv29 :::::::::::::Rv29SP6.seq::::::::::::: TCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAA (SEQ ID NO. 371) GCTATTTAGGTGACACTATAGAATACTCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGT GATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGT AGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCA CTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCA CGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCAGGACCGAATGCTCCGGGAAACATGTCA :::::::::::::Rv29T7.seq::::::::::::: CAGGCATGCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCCCGGGTCGGGCGCCGGGCC (SEQ ID NO. 372) CGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGG GCGGAGCTCCATCCGCTCGGCCGCCAGTGTCCGGGCCCTC Clone Rv2 :::::::::::::Rv2SP6.seq::::::::::::: CCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA (SEQ ID NO. 373) CGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCCAATCCCCCTGCCCTGATACGCGTCGGCAACCGTGAA CGCGATCTCGGCGACCGTCGGATCGGTTTCATCCCGCACAAAACGCGCGTCGGCTACGGGGTCGCTTCCGTCGGTCAC CACCCAGACGAAGTGGTCGACGTAGTCGACTTCCGACAGGTAGTGCATCAACGCCGGACTGGGAACACNAGCCGACAT GAACCGTCGATACAGCGTCTCNCCGGAGAACTGGATGTGTCCGTGCACGGTCCGCTCGCGGTCACCGGGCAGCACGGG GCGTAACATCAGTTGAGTCCCGTCGGCAAGCCGTACCGGAATCGGGGAGACGA :::::::::::::Rv2T7.seq::::::::::::: CAAGATGATCGCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCGAACGTGCTTTCCGGTCCGGCGACCACCATGTC (SEQ ID NO. 374) GCACGCACCGACCAGGCCGAACCCGCCGGCCCGCACATGCCCGTTGATGGCGCCGACCACCGGCAGCGGCGACTCGAC GATGGCGCGCAACAGCGCCGTCATTTCCCGCGCCCGCGCCACCGCCATCCGGTACGGATCACCACCACCACCGCCGGC CTCGCTGAGGTCCGCGCCGGCGCAGAACGTTCCGCCGGTATGCCCCAGCACGACCAGCCGCACCGCCGGATCTGCTTC GGCCGCACTCAGCCCTTGATGTAGTTGGCTGACCAGCGTGCTCGACAGCGCGTTGCGGTTGTGCGGAGAGTTCAGTGT CAGCCTGGCGAAGGGGCCGCCGCAGGCGGCCGGGCCAGCGTAGTCGACGGGGCTG Clone Rv301 :::::::::::::Rv301SP6.seq::::::::::::: CTCAAGCTTCGATCGACAGTACTCCCGCCTTGGGTCTGGTCTTCGAGCTGGTCGGTCATGGTCGGACCTGCTCGTAGT (SEQ ID NO. 375) GGGGATCTAACGCAACATGGTCGGGATTCATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAAACCCCGC GATGCCGGGATTTCCAGCCGCACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCGGACTTCGGGATGTTCGGTATACC ACCGATCGGCAATCTTGCNTATCCGCCGATGCTCGAACGCTAGCCACCCCAAACCAACCACTGTGACNACAATC :::::::::::::Rv301T7.seq::::::::::::: TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCCGCAACGCTGGGCGGATTGGC (SEQ ID NO. 376) CCTGCCGCTGCAGCAGACCATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCC GCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCGGAGGTCGTGCCGATCGATGTGTCCGTCGACAT TCCGG Clone Rv302 :::::::::::::Rv302SP6.seq::::::::::::: TACTCAAGCTTGAACGCTGCGAGCGAGCCCATGTAGAGCGTTTGGTACCAAACCGATCGGTGGGCCAACTTGCCATGG (SEQ ID NO. 377) GCTCACAGCGGCTATCGCGAGCGTGTAGCCGATCATCCGCCAGGCGACGGTGGCCTGAGCGGCAGGGGTTGCCTTATC CATCCTCTTGCGGCATGGTTGCCGCAGGGAGTGCCGGTAAGTCTGGTCGGCAACCTGGCCCGCTGCGGGTTGGGTTCG GATTCCCTCGGCTAGTAAGGTGCTCGCCTGGTGTTACAACGAATCGCTAGACAGCTCTTATCGGGAGTGGCCGTCGCG ATCGTTGCGCTGCCGCTGGCGATCGCGTTCGGCNTTACCGCCACCGGAACGTCCCAAGGTGCGCTCATCGGGCTCTAC GGCGCCATCTTCGCCGGATTCTTCCCNGCCGTGTTCGGTGG :::::::::::::Rv302T7.seq::::::::::::: GCGGTGTCTGAACTTCGCCCGTTCCCTCCAGCGCATTGAGCTTCAGCCCGACCGGCAGGTAGGGAGTCGGCATGCGGT (SEQ ID NO. 378) CCTTCGCCCCGACCCCGCTGGCTAAATAGCCACCCCCGAGCGCGGTCACGGTCTTTGCACCGGGACGACGGCATACCG GCAGCGCGAACATCGCCGCGGGCTGCAGCGTGAACGTCGAATACGAGTCGAACAGTGTCGGCGCGTAAAAACCCGAGC CGGCGGTCGCTTCGGTAATCAACGGCTCCTGCGCAACCAGCTGCAANTCNCCGGTGCCACCGGCGTTGACAATCTTGA TNTCGGCGACCTCGCGCACCAN Clone Rv303 :::::::::::::Rv303SP6.seq::::::::::::: TACTCAGCTTCGGCTCAGGTGGTGCTGCTGGTAAAGTTCNCTGAACGGTGCAGGTTTCGACAATGTGGTGCCGGTTCG (SEQ ID NO. 379) GCGGGTACTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGCGTTCTT GAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCNTGTGTGCCGC GGATTATCAGGACTGACCTCCTGGCTGACCGGCNTGTTTGGTCNCGATGCCTGGCGCCCGGCCGGCGT :::::::::::::Rv303T7.seq::::::::::::: CATCACCTGGTTCATGAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGG (SEQ ID NO. 380) TCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACG ACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAMCGCGTTCTCCACCGACCGGGCCCGGGTGTGG GGTGTT Clone Rv304 :::::::::::::Rv304SP6.seq::::::::::::: CTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTCGCGGCAGCCCACAACNTGCTGCGTCGGATTGCCGGC (SEQ ID NO. 381) GGCGANATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTG CCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGC AGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGC CAGGATCTGCGTGTCATCACNACGCTCGCCAAGGAGGTTGTTGTGGTGCT :::::::::::::Rv304T7.seq::::::::::::: GCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGC (SEQ ID NO. 382) CGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCT CAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAA GTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACGCCACCCCCGGATCGGTTGTTGGCCAAGGTAATTGGGT CATTCCATTTGACGGGACGCCGACCCCGCAGCCCCAGTACCGCCCACGACCACGCCGGCTGACCCCACCACTGTACGA ACACCAAGGCGACGCCGACCA Clone Rv306 :::::::::::::Rv306SP6.seq::::::::::::: CTCAAGCTTGATGCCGCCTAAACCGAAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGC (SEQ ID NO. 383) CGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCAAGCCATACCGGGCGGA GCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCNAGGTCNATACCGATTTGCGCATCCGCAGCCG CACCCTGGACGACAGAACCGTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACANCTTGGCATCCTGGCGCGATT GGCCGGCGCGGTCCTGGTC :::::::::::::Rv306T7.seq::::::::::::: CTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCAC (SEQ ID NO. 384) ACCTTCAGTTGCTCACCGGAATCCAACCGGTANAANGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCG GGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACNCGCATGGGCCACCATC GCATTCACCAGGTCTGCGCG Clone Rv307 :::::::::::::Rv307SP6.seq::::::::::::: CTCAAGCTTCAATTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCC (SEQ ID NO. 385) ATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCAGCAAACCATCGACGCCATCGAATTGCCGGCA ATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATG TCGGAGGTCGTGCCGATCGATNTNTCCGTCNACATTCCGGNGGTCACCATCACCGGCACCAGNATCGACCCGATTCCG CTGAACTTCGACGTTCTCAGCAGCGCCGGAACCA :::::::::::::Rv307T7.seq::::::::::::: TTAACCCCCGTGGCCTCTACGCCGCCTNCGGGTCGAACATGCATCCCGAGCANATGCTCGAGCGCGCACCCCACTCGC (SEQ ID NO. 386) CGATGGCCGGAACCGGCTGGTTACCCGGGTGGCGGCTGACGTTCGGCGGCGAGGACATCGGCTGGGAAGGGGCGCTTG CCACCGTCGTCGAAGACCCAGATTCGAAGGTGTTCGTCGTGCTCTACGACATGACCCCGGCGGACGAGAAGAACCTTG ACCGGTGGGAAGGCTCCGAGTTCGGGATCCACGANAAGATCCGATGCCGCGTT Clone Rv308 :::::::::::::Rv308SP6.seq::::::::::::: CTCAAGCTTGATTTTGATCATCATGGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCG (SEQ ID NO. 387) TGCTTTCCATGGGCGCCTCTTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGG TGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAAANG AAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGG TGTTCGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGAT :::::::::::::Rv308T7.seq::::::::::::: CGNCCAACCCGAATTGGTTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGAC (SEQ ID NO. 388) ACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCG CCNCNGGCGTTACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACCGC CAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCANACGACAT CGTGGCGAGATTCGCCGG Clone Rv309 :::::::::::::Rv309SP6.seq::::::::::::: CGTGACTGCCACCGGGGCCACTCCGCAGAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGA (SEQ ID NO. 389) CTTTCCGCGGTACCCGCTCAACTTTGTGTCNACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTT CATCCTGACGCCGGAACAAATTGACNCAGCGGTTCCNCTGACCAATACGGTCGGTCCCACGATGACCCANTACTACNT CATTCGCACGGANAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGT TCAACCAAACTTGAAGGTGATTGTTAACCTGGGG :::::::::::::Rv309T7.seq::::::::::::: TCGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGT (SEQ ID NO. 390) TGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAAC TGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGACGTGGTTA GCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCA CTTTCAGTGACCGGTTCAACCGGCTCGAAAAGGCGCTACANAT Clone Rv30 :::::::::::::Rv30SP6.seq::::::::::::: ATACTCAAGCTTCCGCTGGGGCCTGTTCAACCATGGCGATCCCGTTGGTCCCGGACATCCCGAACGAGGACACCGCGA (SEQ ID NO. 391) CCCNCTTCGGTGTGTGATCATTACCGTTGGGCCACTGCGTAACCGCTTGCGGCACAAAGAGCCCGGTCTCGACGTCGG AAAGCTCATCGGGCACCCGATTGAAATGCAGCAGCGGCGGCACCACCCCGTGCCGCAGTGACAGAATTGCCTTGATCA GCCCGACGGTCCCCGCCGATGCCGTGCTGTGCCCCATGTTGCTCTTGGCCGATCCAAGCGCGCAGGGGGTGCCCGCGC CATACACCCGCGCCAGGCTGCGGTACTCAATCGGGTCGCCGATTGGCGTACCGGTGCCGTGCGCCTCCACCACACCGA CCGTTTCGGGCTG :::::::::::::Rv30T7PEG.seq::::::::::::: CAACAGCGTTCCAGCGGCATACCACCGCACATGCCGTGCACCCGGCGCCGGGCGGAGTCGCCGCATAACACANGTACA (SEQ ID NO. 392) CCTTGGGAATCGGTGTGCGCCAGGGATTCNACCGCGGGGTGGGGCCGGCGATCGCGCGCCAGGTCGAGTTGGCGCCGA CCGTGATNTCACCGCCGACGTAGTTGGCGTTGTGGTCCGCCATCCGCGCGGCGGGCACGGCGCGGGCCGCCACCACGA TGTCACGGAAGCCGGGGGCGAACGCTCGACGACCTGGTTACCGTCTCNGTCGCNTCNANCGTGGACCCGACNCCACCT GGGCATATGTCCANAACGGACGNGGCCGGTTTCNTCGATGCNGCCGGGGTCCGCGACNTGCGGACNCNCNGNCACACC ATCCGCCAGTCCGCGTGGCGTCCCGCCGCGACTCTGCCTCGGCCGCGCCA Clone Rv310 :::::::::::::Rv310SP6.seq::::::::::::: CTCAAGCTTTGNCGACGATCGGGCGATGTCGATGANAGGAAACCCCAGCGCACAACCGACNATTTTGGCGTAGCCGGC (SEQ ID NO. 393) GGACNTCTGCTCGATTCCGATCACGTCGGCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCGCCGTC GTCGAGGAACACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATTCGGCGAAAAACCGTTCTACGTGGCG GGTGTACTGGGTGTCCAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAAATGCCGTCGGCCCGCAT CGCGTCAACAACTCCCGGGTGAGTGGAATANCACTTGCCGA :::::::::::::Rv310T7.seq::::::::::::: TCCAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTTTTCCAGGAATTCGGCAAAATCGGTA (SEQ ID NO. 394) AGAGCCTGAAGAATTCGGTATCGCCGGACGAAATCTGCGACGCATACGGGGCAGATACGCTTCGGGTTTACGAGATGT CGATGGGGCCGCTGGAGGCTTCACGTCCATGGGCCACAAAGGATGTTGTCGGCGCGTACCGTTTTCTGCAGCGGGTGT GGCGCTTGGTCGTCGACGAGCACACCGGCGAAACTCGGGTGGCTGACGGCGTGGAACTCGACATCGATACGCTACGGG CGTTGCACCGCACCATCGTCGGCGTGTC Clone Rv311 :::::::::::::Rv311SP6.seq::::::::::::: CTCGTCCTTGACTACGCCCAGTATCGAAANCCTCCTGTGCCGGTNCGCTAAACACCCGGCGGACACTCANACGGTGCT (SEQ ID NO. 395) GGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCCGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGG TCGTGCGCAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCG CTGCCACCANACNATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACAACGAGCCCNCCCTGACCGAAGAGTC CTACGCCAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCTTCG :::::::::::::Rv311T7.seq::::::::::::: GTATCGCCTCCNCCTTTGGCCACCAGCAGCCACAGCGCGGTTCGCGGACCGAACGTGGACATCAATAGCCCGGAATCG (SEQ ID NO. 396) GTGTGTGCAAGTTGGTAAACGGTGTTGATCCCAAGCTTTGCCAGCCTTTTCGTAGTCTTGGGCCCCACACCCCACAGT GCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCTGATAGATGCCAGCTGGTTTGGCCAAC CCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACT TTTCGGATCTCTTCNGCGAACTTCCAATGGGGGTCTCCGGGANT Clone Rv312 :::::::::::::Rv312SP6.seq::::::::::::: CTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCCTTGGCCACCGGCGGCGGCTCTCCGGGAAATGGCGG (SEQ ID NO. 397) GTCCCCGGTGGTTTTGCTGANGANTGCTGAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAG CGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGG TGCGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGGATCNCCCGGCTGGGCATTCGGCNTNTTGGCGGCGGCCGGTGG TGGGGGGGCAACANGTGTCCCGGTGCGGGTGGCGCTGC :::::::::::::Rv312T7.seq::::::::::::: ATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGT (SEQ ID NO. 398) CGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAG CGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAG AGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACC TGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCC Clone Rv313 :::::::::::::Rv313SP6.seq::::::::::::: CTCAAGCTTGCAATGCGGGTCGGGATGCCCATGGTTGGAANATGGTCGCCCTGGCGTCNAATACGCGCGAGCGCATGA (SEQ ID NO. 399) GCTCACCGGTTCGGAACAACGTATCGAAAAACGTCGCACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTA TCCCAACTCTAACTGTGCTATCGGATCAGCGTGAATATCGANATATTGCGAATGCGATGACAGGCCGCCATTCGGTTT ATTCGCTTACGCTTCCCGGGTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTA ACGCAATTATTGATGTGGTAGGCGGCAGCTGCCGTTTTGTGCTGTCGG :::::::::::::Rv313T7.seq::::::::::::: CAAATACACGCCGGACGCACAGGCGGACATCGCCATCCCGAGCACACCCAAAACGGGATACAGGATCGAGGCCAACGC (SEQ ID NO. 400) CACGGCCGCGCCCAGGATCACCAACCACACCGGCTTGGTCAGCTTGTCGGCGCGGTATAGGCATCGGGCCGCTGCCAA CGCAGCATGCACAAACGCGTACACCGCTGTCACCAAGACGGCGACCAGCAATACCAGCATGACGGTACCCACGAGGTG GCTCACGCATTCAGACTATGCGGTTTGCATCCAACACG Clone Rv314 :::::::::::::Rv314SP6.seq::::::::::::: CTCGTCCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCGGTGATCGCACTGTCG (SEQ ID NO. 401) CCGATCGGCGCCGCACCTGGTTGGTGTTACGGATGAATCCGCAGCGAAATGTGGCTGCGGTGGCGTGTCGTGACTCGT TGGCGTCGACGCTGGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGGGCAAAGTTGTGCGGCCCGGCCGGTGA CGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGACATGGAGTCGCCCCGGTT :::::::::::::Rv314T7.seq::::::::::::: GTCTAGNCCGCCGAACACGATACGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGT (SEQ ID NO. 402) TTTGCTGAAGANTGCTGAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAA TCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTC GGCCGGCGGAAGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGG Clone Rv315 :::::::::::::Rv315SP6.seq::::::::::::: ACTCAAGCTTGAGATTGGCGTCAACGGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGC (SEQ ID NO. 403) CCGATGCCGCGGCCCTCGTGGCCACGCATGTACANCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCG TCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGC ACGTCG TCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAAATGCTGGTGTAN CCGATGGCGCGAAACTCCCCATGACAANTCGGAATCCCGCGCCTCGGCGACCCCGCTCAATGTTGCTTCTCNTGCTTG :::::::::::::Rv315T7.seq::::::::::::: TCGACNAGCATTCTTGACNGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTC (SEQ ID NO. 404) CGGTAGCGCTCGTCCGGGTATGCTGCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAGAACGCCACCGGTCCAGC GCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAACAGGGGACATGCTTAGCCGTAGGGCGCGCGCCAAGGC GGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCG GTCATTGATCATCTTGCGCACCGCGGATAATCCGGGAT TG Clone Rv316 :::::::::::::Rv316SP6.seq::::::::::::: ACCGGGGCCACTCCGCACAATCTGTACCCGACCAANATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGG (SEQ ID NO. 405) TACCCGCTCAACTTTGTGTCNACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACG CCGGACAAATTGACGCNGCGGTTCCGCTGACCAATACGGTCGGTCCCACNATGACCCANTACTACATCATTCGCCACG GANAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAAC TTGAAGGTNATTGTTNACCTGGGCTACGGCGANCCGGCCTNTGGTTATTCCACCTCNCCGCCCAATGTTTGCNACTCC CGTTCGGGGTTGTTCCCNNAAGGTCAACCC :::::::::::::Rv316T7.seq::::::::::::: CGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACAGCCCATGTT (SEQ ID NO. 406) GGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACT GGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGACGTGGTTAG CGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGANCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCAC TTTCAGTGACCGGTTC Clone Rv317 :::::::::::::Rv317SP6.seq::::::::::::: CTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTCGCGATGCCCAGCAAGGAGTC (SEQ ID NO. 407) ATCGCCGCCGAGCTTGGCCAGGATCTTGTCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTAGAC ACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGCGTACAAACTCGCTGACGTCGTGGCC GCTGTCGCGAATGCCCTCGAGCATCTCCAGGACAGCCTGATTGGCGCCGCCATGAAGCGGACCCCATAGTGCGTTGAT GCC :::::::::::::Rv317T7.seq::::::::::::: GGTCAGGCCGAGCAGGCGCGAGGAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGC (SEQ ID NO. 408) CACAACGGGAAGATCGCCTTGAGCGTCGCTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATCA TGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCNACGAGGTCTCGGTGCCGCCGCGGCCAGGGCACCA GCAGTGACGAGTCCAGGCGCCGTCGGGCCAAGCAGTCGCGGTGCCANCCGTGGTGGGTCGGGCGATGGTTGGGTGTGC TCATTTCGGGAACGCCA Clone Rv318 :::::::::::::Rv318SP6.seq::::::::::::: CTCGAAGCTTTAACAGCATCAACCCCGCCCCGCACCAGCACCGACACNATGTCGATGCCATCGAGGTGAATGTCGAAC (SEQ ID NO. 409) TGGCGCAAACCATCGGCGACCGCGACCACCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCCGACGGG CCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCANCCGGCCGTTNTNNTCACGCACCCCTACCGTGTCACGCCCA AAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCNCACCCAGTGTCGTGCCCGGATCC :::::::::::::Rv318T7.seq::::::::::::: TGATGCCGCACCCGATCGACGGTCGTTGGTCGGGGTTGACTGGCCGCCCGGCGAAGCAGGGCGTCGACCGCGGCCCGG (SEQ ID NO. 410) ACGTCGGCGGCCGTCACCGGTCGGCCATTGCCCGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGG CCGTCGAAGACNAACGTGTCGGGTGTGCAGGCCGCGGAGAAGGCGCGGGCGACNTCTTGGGTTTCGTCGTANAGATAC GGGAACGTCCAGCCGTGGCGGCGGGCCTCGGCGACCATCTGATCGGGCCCGTCC Clone Rv319 :::::::::::::Rv319SP6.seq::::::::::::: TTTCGGGCGAGGCGGTATANCTTCCCNTCGTACCGGCGACCGCCAGCCGANAAGCTCGTTTTCCCAGTGTTGCTGGGG (SEQ ID NO. 411) ATTCTCACGCTGCTGCTGANTGCGTGCCAAACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACNATCGTGCG ACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACNACTCCAAGCTGGCGCCGTCT CGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCNTGCCANCGCTCCGAGTTGC NTGCGGATCGACTACNAATTGATCACCCANAACCATCGGGCGTNTTACTGCCTGAAGTACCTGGTGCGGGTCGGATAC TGCTATCCGGCGGTGACAACCCCGGCAAGC :::::::::::::Rv319T7.seq::::::::::::: GTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATG (SEQ ID NO. 412) CTGCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAGAACGCCACCGGTCCAGCGCGTGGGCCGCCGCGGTCCCCA TCACAAACTGAACCCCCAACAGGGACATGCTTAGCGGTAGGGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCT GCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCGGTCATTGATCATCTTGCGCACCG CGGATAATCCGGGATTGCCAGCCCATTCNACTACCGCATGCGAGTCATCGGCTGACCGCAGCGGTC Clone Rv31 :::::::::::::Rv31SP6.seq::::::::::::: TCGCCTAGGCGGGCTTCCCCTTCCGTCCGAGCNGTCAGAAGCTCCTATGACAATGCACTACCCGAGACNATCAACGGC (SEQ ID NO. 413) CTATGCAATACCNAGCTGATCAAACCCGGCAAGCCCTGGCGGTCCATCGAGGATGTCGAGTTGGCCACCGCGCGCTGG GTCGACTGGTTCAACCATCGCCGCCTCTACCGGTACTGCGGCGACATCCCGCCGGTCTAACTCGACGCCGCCTCACTA CGCTCAACGCCAGAGACCANCCGCCGGCTGACGTCTCAGATCAGAGAGTCTCCGGACTCACCGGGGCGGTTCATCCCC ACTGTCGATAGCGTCTGTGGATAACTTTGTCTGCA :::::::::::::Rv31T7.seq::::::::::::: GCGCGTNGAACTGATAGGTGCGGCCCGGCTCGAGCANGCCGGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGG (SEQ ID NO. 414) TGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACAATCTTGGCGTCCACGGTGG TCTGGGTCA Clone Rv321 :::::::::::::Rv321SP6.seq::::::::::::: CTCAAGCTTCAATACAGAGTTATAAACTGTGATAATCAACCCTCATCAATGATGACNAACTAACCCCCGATATCAGGT (SEQ ID NO. 415) CACATGACGAAGGGAAAGAGAAGGAAATCAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTC AAAAAGTATGAGAAAATCCATGCAGGCTGAAGGAAACAGCAATAACTGTGACAAATTACCCTCAGTAGGTCAGAACAA ATGTGACGAACCACCCTCAAATCTGTGACAGATAACCCTCAGACTATCCTGTCGTCATGGAAGTGATATCGCGGAAGG AAAAT Clone Rv322 :::::::::::::Rv322SP6.seq::::::::::::: CTCAAGCTTCGATCGACATTACTCCCGCCTTGGGTCTGGTCTCCGAGCTGGTCGGTCATGGTCGGACCTGCTGGTAGT (SEQ ID NO. 416) GGGGATCTAACGCAACATGGTCGGGATTCATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAAACCCCGC GATGCCGGGATTTCCAGCCGCACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCGGACTTCGGGATGTTCGGTATACC ANCGATCGGCAATCTTGCGTATCCGCCGATGCTCGAACGCTANCCACGCCAAACCAACCACTGTGACNACAATCGCCA CCACACCAAAGGTCATGCCCTCGGCGTGATGTCCGGTGCCGAAAGCCGCAAGAGCTCCGACGCCGCC :::::::::::::Rv322T7.seq::::::::::::: CATTCCCAATTGAATTTCCCNATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGG (SEQ ID NO. 417) GCGGATTGGCCCTGCCGCTGCAGCANACCATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCA TCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCGGAGGTCGTGCCGATCGATGTGT CCGTCGACATTCCGGCGGTCACCATCACCGGCACCAGGATCGACCCGATTCCGCTGAACTTCGACGTTCTCAGCAGCG CCGGACCCATCAACATCTCGATCATCGACATTCCGGCGCTGCCGGGCTTTGGCAACTCGACCGAGCTGCCGTCGTCGG GCTTCTTCAACACCGGCGGCGGTGGCGGCT Clone Rv327 :::::::::::::Rv327SP6.seq::::::::::::: CTCAAGCTTTCGGCGGAGACGGACANNTTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGG (SEQ ID NO. 418) CCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCC GTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTGCACG ACATTAAATGTCACGGTATTG :::::::::::::Rv327T7.seq::::::::::::: AGCTTAACTGCTCCCTAATACCTGGGGCTGTGCCTGCGGTGTATGCACGGCATACGGACATCCNTCCCCTGAGACCCN (SEQ ID NO. 419) CGGTCTAATCAGCCACGTGTCCACCATCAGGGGTCAACCCCGGCCAAGGGCGACGGCACCCCAAGTTCGCCGACCGTT AACCTATTGCTGTGAGCTTCATTTGCTGCGAGCAAAACAGTTGGTCGGCCGTTAGGAACTGAATTGACACTCAACCGA TTTGGTGCCNCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACGACCACAATGTGACCGGG GGAGGTGCAACCACTGGCCACGCGTCCGCGAATGTCTATTGCGGGGG Clone Rv328 :::::::::::::Rv328SP6.seq::::::::::::: CTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAG (SEQ ID NO. 420) AAGGATTCGCTGGAGCGGTGGCTGTCCAAAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAAC CGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAAACGTTGTGGGAGTTCCTG CCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCC CT :::::::::::::Rv328T7.seq::::::::::::: GCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACCTGAACGG (SEQ ID NO. 421) GCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACG GGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCACAGACGAGA GGACTCGATCCCATCTAGGTGTGGACGAAACAGATCTTCTGTCCGA Clone Rv329 :::::::::::::Rv329SP6.seq::::::::::::: TCGCCTCCGCATATGGGTCGACGCCAAGCGGGTCCGGATTTCTGGGCTTCATCGCTCGCGCCGTCGCGACAAACAGCG (SEQ ID NO. 422) CGGTCGAACCGACACTCGTTGTGATGTCCCAGCTATCACCTTCGGTACGCACCCAATCGACCCTACNCGGCTATCTCA GCCGCGATCTCCAGGCTCCGCCGAGCCAGGTGCATCCCGGTCCGGATCCCACTAACCCGGCACCATTGGCGTCN :::::::::::::Rv329T7.seq::::::::::::: GTCCTCGAGTGCCGCCGTCGNCACNCCCAGCGCCCGCGCGGCCACTTGGATGCGACCCGTTTCAAGTCCCTTCATCAT (SEQ ID NO. 423) CTGCGAAAAGCCTTGACCCATGGCTCCGCCCAGGATCCCCGAGACCGGCACCCGGAGGTTGTCGAACGACAGCTCGCA GGATTCGACGCCCTTGTAACCCAACTTCGGCAAGTCCCGCGACACCGTGAGTCCCGGCCCGGGTTCGACGAGCACGAT CGACATGCCTTGGTGCCGCGGTGTGGCGTTCGGGTCGG Clone Rv32 :::::::::::::Rv32SP6.seq::::::::::::: GGCATACCAATGTGGACTTCTGCTCACCCACGATATCCGTGGTCTGATCCGCTGCTGCGGCGGGCTGCNACCTGCNTC (SEQ ID NO. 424) TCNGCGGCACCCGTNACTACATGGCNCGCGCCGCACGCATACGTCGCGGCGGGACCCACTCCNACTGGTCGACGGTGC TGGCCGCGTGTCCGCANGTCCCNAACCCGGCCGCACCGACGAAACCGGCCGCCGTCCGTTCTGGACCAACGCTCATGT GCCGTCGGGGTCCATGCTCGACGCCATCGAGACCGTAACCAGCGTCCTCGAGCGGTTCGCCTCCGGCTTCCGTGACAT CTTCGTGGCTGCTCGCGCCGTGCCGCCGCGCGGATGGTCGACCACAACGCCAACCACCTCGGCGGTGACATCACCGTC CGCGCCACTCGACCTGGCGCGCGATCGCGGCCC :::::::::::::Rv32T7.seq::::::::::::: GTGAGCAGACCTACGCCNCCTGGTTGCGCCAACTCGGTACCGATCATGGCGCGCNGCCTGTCGTCACCGATACCCAGC (SEQ ID NO. 425) GAACAAGACAGCCCGGTCCGCGACAAGATGACTTTCCCGATCTCTTCGGCGACTTCCATGGGGTCGTCCGGAGTCCCG GGCGCCACCGCGAGGTAACCCTCGTCTCAGTCCCATACGCGACCGGGTATCCACGTCGCGCAACAACGCCACCACCTC CCCAGACGCCNCGTTGTACGCGGCTGGGTTCCACNGCAATAAGTGGCCTCANGGCATCGTCCGGCGGCGGTCCNCAAC GCA Clone Rv330 :::::::::::::Rv330SP6.seq::::::::::::: CTCAAGCTTGAGGTTAACTTTGAACGGATCGAGCTGGACGTTCGAGACGGTGATCGGGCCGAACCTGAATTGTCCGGT (SEQ ID NO. 426) AATGCCCAACGCAAAAAGCAGGGTGGTGGCCGGGGCGGTGAAACCGGCGTCGGCGGCACCGTCGAAATCTATGTGGAT TGCCGGAATGGGGATGTCCGGCACGGCGAAACCGTAGTTCGCTTGTCCCGTGAGGCCCACGTGGATGGGGGGAAAGAT CCTGGTGTCCGGGATAATAATGGGGCCGATGCCGCCGGTTGAAGTCCACTGGATCGGGAATTCCGGAATCTTGATCCG ACGTTCAGGCCGAACAGGCCCTC :::::::::::::Rv330T7.seq::::::::::::: CGGCGACGTCGCGATACGCCGAGCAGTTGGGAATCGCTCTGCAGCAAACCAATATTCTGCGCGACGTTCGAGAGGACT (SEQ ID NO. 427) TTTTGAATGGACGGATCTACCTGCCGCGCGACGAGCTGGACCGATTAGGCGTACGCCTCCGCCTGGACGACACCGGGG CACTCGATGACCCCGACGGACGGCTCGCGGCNCTGCTGCGGTTCAGTGCCGACCGCGCCGCAGACTGGTNTTCGCTGG GACTGCGGCTGATTCCACACCTCGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCAGC TCGCCTTGATCAGAGCATCGCCGGCGGTCGTCTA Clone Rv331 :::::::::::::Rv331SP6.seq::::::::::::: CTATAAAATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCG (SEQ ID NO. 428) GGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGNTGCGCTACGTCNAGCCATA CCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCNAGGTCNATACCGATTTGCGCAT CCGCAGCCGCACCCTGAACNACANAACCGTGCCCTACTATTGCTTGTCNGGCGGGGCCAAAAAACAGCTTGGCATCCT GGCCCNATTGGCCGGCGCGG :::::::::::::Rv331T7.seq::::::::::::: CTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTGCTCGGCATCTTCCATAGCCCGCCACACCTTCAGTT (SEQ ID NO. 429) GCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAG AGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCA GGTCTGCGCGAATCNCCANCACGTANACNGTTCCTTTCCTAA Clone Rv333 :::::::::::::Rv333SP6.seq::::::::::::: CTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACCGAAC (SEQ ID NO. 430) GGGCGAGCCGGGAGTCTGGTNCGCATCGAACAAANAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGA CGGGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCNAGTGGCGCTCACACTCCAGGTACTCGACCTCACAGACNA AAGGACTCNATCCCATCTAGGTGTGGACNAAACAGATCTTCTGTCCGACNACTACACCACCACCCAGGCCATCGCCGC CGCCCGCGATGCCAACTTCGACGCCGTACTGGCCCCGGCGGGGGGCGCTCCCCGGTTGTCAACACTTGCCGTGTTCNT TCACGCNCTGCCCCACATCCAACCCCAACG Clone Rv334 :::::::::::::Rv334T7.seq::::::::::::: GTTCTTGGGCCCATGCGGAGGTATCGCCGTTTCCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCG (SEQ ID NO. 431) CTTGTGCAGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGC Clone Rv335 :::::::::::::Rv335SP6.seq::::::::::::: CAATACTCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCA (SEQ ID NO. 432) ACNACNACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAAGTAC AACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATC :::::::::::::Rv335T7.seq::::::::::::: CNTCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTC (SEQ ID NO. 433) TTTCGGGCTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGAT CCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAA CACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCCGTTACCATGTC GTTGTTTGTCTTCACCCATTTGCGAATTATTGGTCAGATCGGTACCAC Clone Rv336 :::::::::::::Rv336SP6.seq::::::::::::: ATACTCAAGCTTTTACGGTGATCGCNCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCA (SEQ ID NO. 434) ACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCNACAGCCGCCTGACCCTGAAAC CAGCTTCCATATCCCGCGANNAACGACGCCAGTCCGCTACGTNACCCCTCCGCGACTCTCCATGGACAACAGCCCCTT CTCCACCGACCGGGCCCGGGTGTGGGGTNTT :::::::::::::Rv336T7.seq::::::::::::: GCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGC (SEQ ID NO. 435) GCAGGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCAT GGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCT GACCGGCATGTTTGGTCGCGATGCCTGGCG Clone Rv337 :::::::::::::Rv337SP6.seq::::::::::::: GCTTTCCGCCGATACCCGCCATGTCNCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAG (SEQ ID NO. 436) TGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGC GGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCANCACTCAATGC CGACCAGGCCGAGGCCGGANACAAAANTATCGCTAAGGTCACCGCGATCACNAGCATGGTGATCGCAGCAATGTTGCT AGTGATCTATCGCTCCGTAATTA :::::::::::::Rv337T7.seq::::::::::::: CTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGAC (SEQ ID NO. 437) ACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCG CCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGC CAAGGCGGCGTGCCANGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCA Clone Rv338 :::::::::::::Rv338SP6.seq::::::::::::: TACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACT (SEQ ID NO. 438) TGAACCTGGTCTCGTTCGGCAATAACTCGTTCGGCGTGCAGGACGCGGCGCAAACGTNCTTCGGCATCAACGCGTCCG ANCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCNACCAGCACGCTCAACCCGTA :::::::::::::Rv338T7.seq::::::::::::: CCCACGACTTTCTCCTCGATCACTTGGATTTGTACGAAGAGGCAACGAAAGCAGTGATCCTCGGGATGGTCGACGCCT (SEQ ID NO. 439) ACATCGACCCGCCGTTCACGCCGCACAGCCTGCTAGATGCGCTGGGCGAGCAGGTCCCACAGTTCGCCGCTAAGGCAC GGCGTCTGTTCCCGTCCGGATCGCCATTCGGCCTCGGCGTCCTGCTCCCATTCGATCAATAGGGCTGGCAGCTCCGTC GGCAGGGGCCTACGCCTCACCCCGTCACG Clone Rv339 :::::::::::::Rv339SP6.seq::::::::::::: CTCAAGCTTATGCGCGCCGGCCGAGGTCTGCTCACGGCAACCCCTGAAGTTTAGGGGACNACCTACTCAGCGCAAAAT (SEQ ID NO. 440) TTCGCTAATGTGAGTCCGCCCCACCAGGGGNANATCAACCCATGTCGATCATGATCTACCCGGATACCGGATTGGCGG TAGCGCCCACGATCGTCNAAATNTCCGCCTGAATCATCGGATAGCTGATCCGGCGTCAACGCGTTTTGANTTCACCGC GCAACAGCCGCCAGGCCGGCCCGCANCGANCCGATCTCNTCGGGCCGCATGGGCCCCAATCTTNTCG :::::::::::::Rv339T7.seq::::::::::::: GTGTGTGGTGGAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGACAGCTCCCAATTGACGTGAGCCCGCTCACTTGC (SEQ ID NO. 441) TGGGTAAGCGTC Clone Rv33 :::::::::::::Rv33SP6.seq::::::::::::: CTTTACACTTCCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATG (SEQ ID NO. 442) ACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGGGCGTGACGGCCACCGGGGCCACTCCG CACCATCTGTACCCGACCAAGATCTAC :::::::::::::Rv33T7.seq::::::::::::: CAGGCATGCAAGCTTTAGCTGCCCGAATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCACATAAAGCACGAACA (SEQ ID NO. 443) GGCGGGCAAAACGTCNATCTCGGAGCCGGAAGGGCAATCAGCCGACCGTCGACGAACGACACCGGCGAGACCACTTAG GCAGTGACGGCCGGCCCGAACATTACGCGCTCGTTGATTAGGCGTTCGGTCTCGTCCGCGGTCATGCCGAGCAGCTTG CGGCAGATCTGAACGCTGTCCTGTCCGGGCAGCGGCGCCGGGCGTTGGGGTGCCTGCCCGAATGTGACGAAACGGAGC CGGACCCGTCTCGGCGGGCCGCGGACGGCGATCCGC Clone Rv340 :::::::::::::Rv340SP6.seq::::::::::::: CNCAAGCTTGCGGATGTTACCCCTGACAGCCTGAACTATGTCNAAACACACGGCACCGGAACGTTGTTGGGGGACCCC (SEQ ID NO. 444) ATCGANTTCGAGTCGCTGGCGGCCACTTATGGCCTGGGTAAAGGCCAGGGCNANAGCCCGTGCGCATTGGGGTCGGTC AAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATNCATCAAGGCGGTGCTGGCGGTGCAACGTGGG CACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCNACGCGTCGGCNACGCGGCTGTTCGTGCCNACC NAAAACCCCCCGTGGCCGGCGGC :::::::::::::Rv340T7.seq::::::::::::: GGAACCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGGTGCTGCGCGCTGCCCAGTAG (SEQ ID NO. 445) TGCACCTTCTTGACGCCTCGAAAAGGGGAGTCGGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCNA TAGCCGGTCTCCTCGAGTATCTCCCGCACCGCCCCCACCGGTGCGGTCTCACCCANATCCACTTTGCCCTTGGGCAGC GACCAGTCGTCGTANCNGGGGCGGTGAATGACAACGATCTCGACCGGCCCTTCCN Clone Rv341 :::::::::::::Rv341SP6.seq::::::::::::: TACTCAAGCTTCAGAACAGGCCTGTTGTGGGCNCACCCGGCTCGCCGAGTTCTGCACCCACCGCCTCAAGTGCGGCCC (SEQ ID NO. 446) GCACCGCCGGCATCTCCCGGTCACGCAGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAA TGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATCGCGGTGCTCAGCAGCGTCTCGA CAGCCACCACCCGAGTGGCGACCAGCTGC :::::::::::::Rv341T7.seq::::::::::::: TAATGTCTTGCCGACGTCACCACAATCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCG (SEQ ID NO. 447) AGCGGCAGCTCGATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGG ATAGTGACGAAGGCAAGACCTATATCTGCCGTCGGAAGAAGAATCGAGTAGCCGGTCGACACAACGGAAGCGAAAGTG TCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGCGGC Clone Rv343 :::::::::::::Rv343SP6.seq::::::::::::: TACTCAAGCTTTCGTCAGTTCATCGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAA (SEQ ID NO. 446) CGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCANATGGCCAGCGCGTGCCGGGC CACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGCCT CGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGANNATCACATCGCCGCGATCGCCCTGTTC :::::::::::::Rv343T7.seq::::::::::::: CCACCCGTGTAATTTGGGATGGGCNAAAAGGCNAACCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGG (SEQ ID NO. 449) CTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCCTCGCCCTCGGACCGCGAACATTCGGGG ATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTAC AGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACG Clone Rv344 :::::::::::::Rv344SP6.seq::::::::::::: TCAAGCTTTAGCTGCCCGAATCCGTCANCCCGATGCNCCCAGATCGGGGCTTCGCANATAAAGCACNAACAGGCGGGC (SEQ ID NO. 450) AAAACGTCNATCTCGGAGCCGGAAGGGCAATCANCCGACCGTCNACAAACGACACCGGCGANACCACTTAGGCAGTGA CGGCCGGCCCGAACATTACNCGCTCGTTGATTAGGCGTTCGGTCTCGTCCGCGGTCATGCCGAGCAGCTTGCGGCANA TCTGAACGCTGTCCTGTCCGGGCAGCGGCGCCGGGCGTTGGGGTGCCTGCGGAATGTGACNAAACGGAGCCGGACCCN TCTCGGCG :::::::::::::Rv344T7.seq::::::::::::: CCGGGGCCACTCCGCACAATCNGTACCNNACCAANATCTACACCATCGAATACGACGGCGTCGCCGANTTTCCGCGGT (SEQ ID NO. 451) ACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGC CGGAACAAATNGACGCNTCGGTTCCGCTGACCAATACGGTCGGTCCC Clone Rv346 :::::::::::::Rv346SP6.seq::::::::::::: NCTGGCCTTTGGTCCACACTAANACAATACTCAAGCTTCCGGCCGCAGAGCCGCCAACTCACGATATCGTTAACCGAT (SEQ ID NO. 452) ATCCCGAGCCGATAGCTGGCGGGCTCGGGTGGTGGCCAGCGGCGCTGCGACNAAAGGTGTGACCGTCATGAAACAGAC ACCACCGGCGGCCGTCGGCCGTCGTCACCTGCTCGANATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGCGTG TNGTGGGTCNCCGCCCGAGCCCGGCAAAGGCCGGCCCGACACAACCCCGGAAC :::::::::::::Rv346T7.seq::::::::::::: CATCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTGGTGGTCAGCATCGTGGCCGGTCTGCTGTTG (SEQ ID NO. 453) TATGCCAGCTTCCCGCCGCGCAACTGCTGGTGGGCGGCGGTGGTTGCGCTCGCATTGCTGGCCTGGGTGCTGACCCAC CGCGCGACGACACCGGTGGGTGGGCTGGGCTACGGCCTGCTATTCGGCCTGGTGTTCTACGTCTCGTTGTTGCCGTGG ATCGGCGAGCTGGTGGGCCCCGGGCCCTGGTTGGCACT Clone Rv347 :::::::::::::Rv347SP6.seq::::::::::::: GACAATACTCAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGG (SEQ ID NO. 454) GGTGTTGATGTCCCAGCCGCTGAANTCGTCCTGCGCGCGCAGGCCGTCNAACAGGTACAGGGCGGGCGAATTGGCACC ACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCGACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGC CCGGGAAAATGCCCCCGCGGTCNCCGTGCCACCGACGGCGCCCANCAAACCCGACACTAGGGCCGCGCCNACGGCCCC GACCACNANTCNACGCGACATACCCGTGACGGCGCCACNAACCCTGTCAACA :::::::::::::Rv347T7.seq::::::::::::: CCTCCAACTCGGCGGGGAAGCGACNCCAGCCTACCGAGCTTGGAGTCCANGACGCCAGCGGCGGCGTCGGTCTGCGTC (SEQ ID NO. 455) GTGGTGCCGCCGGGGTGGCGTTGGCTGGCAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATANACG CGGGCCGAGGCCGGTGCGATACCGTATTGCGTCAATTGGGACGCGGTTGTGCATTCGGCTAGCTCGGTTGCCACACCC GTCAGGGGTTCGACGTTGGCGGGTTCGGCGGGCCCCANCACCGCTGTCACCATGCCCGCCAAGCCGACCTGCGGCGCC ACCAACTGCAGCACCANCATGTCGCCGTCGCGCGCCGCGATCACATGG Clone Rv348 :::::::::::::Rv348SP6.seq::::::::::::: CTCAAGCTTTTTGAGCGTCGCGCGGGGCANCTTCGCCGGCAATTCTACTANCGAGAANTCTGGCCCGATACGGATCTG (SEQ ID NO. 456) ACCGAANTCGCTGCGGTGCANCCCACCCTCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTT GCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTT GCGCCGCNAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCNCCGCCGCGGCACTGCACCGCCAGTGCCGCGGCGA :::::::::::::Rv348T7.seq::::::::::::: CNCCAGCTTGATTGGTCTGGTTGCATTGGCCAGCTGCGCGAGCCTGGCTCACTTCAACTACGACGACCGCAAACAATT (SEQ ID NO. 457) GCCGCCTTCGGATCCGAGTTCGGTTGGGTACGCGGCAATGGAGCACCATTTCTCGGTGAATCAGACTATTCCTGAGTA CTTGATCATCCACTCTGCACACGACCTGCGAACCCCGCGCGGCCTTGCCGACCTGGAGCAGCTGGCGCAACGTGTGAG CCAGATCCCAGGCGTTGCCATGGTTCGCGGTGTGACCCGGCCAAACGGGGAAAC Clone Rv349 :::::::::::::Rv349SP6.seq::::::::::::: CAATACTCAAGCTTGACTGGGCCCGCACCTTCGGCGCCACCCACACCGTCAACGCCCGCGAAGTCNACGTCGTCCAGG (SEQ ID NO. 458) CCATCGGCGGCCTCACGGATGGATTCGGCGCGGACGTGGTGATCGACGCCGTCGGCCGACCGGAAACCTACCAGCAGG CCTTCTACGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTTCCNACGCCCGACATGCGCCTGGACATGCCGC TGGTCNACTTCTTCTCTCACGG :::::::::::::Rv349T7.seq::::::::::::: TCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCAATTGACCCCACAGCGGAAATGCCGACTATTCGCAGGCCTC (SEQ ID NO. 459) CTTCGCCTTGGCTGCCGGAGAGGGGCTCCGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGC GTGCCTTGTNTANGATNANCTCGGCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCANATTCGTACACNTGGC CGGCGGCGACATACGCTTCACCGTGGATCTGCTCCACACGGACCGCCCTGTCGGGATCCTGCTCACGGGTAANGGAAC TTACGTGGCACTCGG Clone Rv34 :::::::::::::Rv34SP6.seq::::::::::::: GACCACGCCAGGCTAATCACGTGACGCTACCGAATACCCTNCCTAGTGGTGCAGGCTCCCGCTGGAAATGGCCCTGTA (SEQ ID. NO. 460) CCAACTCGCGCACCGGTGCCAG :::::::::::::Rv34T7.seq::::::::::::: CGGCACCCGACCCCTTTGAGCCGTCCGCCGTGGCCGCGGTGGAACTGGCCGACGAGGGACTGATCGTGCTGGGCAAAT (SEQ ID NO. 461) TGGTCGATGGCACGCTGGCCGCCGATCTGAAGGTCN Clone Rv350 :::::::::::::Rv350SP6.seq::::::::::::: CTCAAGCTTGCCGTTACCCCGACTTCCGGAGGGACACCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGA (SEQ ID NO. 462) CGCAGGCCGGTTGGACTTGTCGTGCTGGACAAGGGGTTTAGCCGCCGAAGCAGTGACGTACATCGGCGAAAAGCAGTT CGCCTGTCGACCGACGGNGCNNACCGTGAGGCTAGGGAAGCGAGGAGCACATGGCCGCCGACCCGCAATGTACACGCT GCAAGCAAACCATCGAACCCGGATGGCTATNCNTCACCGCCCATCGCCGCGGT :::::::::::::Rv350T7.seq::::::::::::: CATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCC (SEQ ID NO. 463) TACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGAC ACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGCCGGA GACAAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCAATG Clone Rv351 :::::::::::::Rv351SP6.seq::::::::::::: ATACTCAAGCTTCGGTACGGTGGCGGGCCGTGCTGCTGGCCGCGGTCGCGGCGTGCGCGGCCTGCGGTCTCGTTTACN (SEQ ID NO. 464) AGCTCGCGCTGCTGACACTGGCGGCNAGCCTGAACGGCGGCGGGATCGTGGCCACCTCCCTGATCGTCGCGGGCTACA TAGCCGCGCTGGGAGCAGGCGCCTTGCTGATCAAGCCGCTACTTGCAGACGCGGCCATCGCGTTCATCGCCGTGGAGG CGGTGCTGGGGATGATCGGCG :::::::::::::Rv351T7.seq::::::::::::: TGTCAAGTCCTTTCAGATCTCNTTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTAC (SEQ ID NO. 465) CGGATTCAAGCCGGTCGGTCACGCCGCGGTGGTACCGGCTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCG CGAAGTGCGTTCGCGCAGCAAGATCGCGGCCGTAATGCCGGCGATGACCGCGATGACCAGCGCGATCCAGGAGAACCG TTCCAACCAGTGCTGGGCGGCCATCCCGGCGAAGTAGACCAGTGCAGTGGTGCC Clone Rv352 :::::::::::::Rv352SP6.seq::::::::::::: CAATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAANTGCGG (SEQ ID NO. 466) CCCGCACCGCCGGCATCTCCCGGTCACGCAGGGCCGCGGCCCGCGCCGCANCGACGGNGTGTTCGCGCAGTTCGCCGT CAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCNCGTTCACTAATCGCGGTGCTC :::::::::::::Rv352T7.seq::::::::::::: TACGCTGGCGCTGGAGGGAGCCANNTACAACATCCACGCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCA (SEQ ID NO. 467) GGACATCCTGCCGCCCGAAGTACTGGAAAAGCTCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCGA GGAGTGTGCCGACAACGCATCGGTGTACGTCGTCGGTGGTGGCAAGGTGCAGCGAGTTGCGCTGTTTGGCAACGACGG CGCCAACTTCGACAAACCGCCGTCGGTACAAGATGTTGCGGCGCGGTGGGCCGAGATCACCGATCTGTCCGGTGCGAA AATTGCTG Clone Rv353 :::::::::::::Rv353SP6.seq::::::::::::: GCTTTTCCCGTCCGTCNNCGCTCAACCGCGTGAGGCCGAAGCGGNTGGTTACGACTCCCTGTTTGTGATGGACCACTT (SEQ ID NO. 468) CTACCAACTGCCCATGTTGGGGACNCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGC GACCGANCGGCTGCNNNTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAANATCATCAC CACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGANCTGGAACA :::::::::::::Rv353T7.seq::::::::::::: CNGCTTTTTAATGGCCTTGACNTGGGCGNGCCGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTAC (SEQ ID NO. 469) ACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGC ACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTC GGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATC GTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTAT G Clone Rv354 :::::::::::::Rv354SP6.seq::::::::::::: CTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCANGGGGCGCCAACGCGGTGTCCGCGCCGTCNA (SEQ ID NO. 470) GCTGAACGTTGCTGCCCGCCTGGAGAACCTGGCGCTGCTGCGCACCCTGGTCGGCGCCATCGGCACCTTCGAGGACCT GGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGAGGTGTGCACCCGGTTGATTCGCTCGGCCTTGCCGGA TGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAANACGAANTTGTGGTGGAGGCTTCTGCTGCCTGCGACACCCACNA CGTGGTGGCACCGGGCAGCTTTAGCTGGCAT :::::::::::::Rv354T7.seq::::::::::::: CCGACGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCGGTCTTGGCCA (SEQ ID NO. 471) ATTGCCGCGGCACCAAGCCGTCGCGCGCCATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATCACCACCG TGGTAAGCCCGGCCAGCGCGCCGACGGAGATGATGCCGCTGGCCCAGTACACCCCGTTGGCCTGGAACGCGGTGGCCA GATTTGCCGGCCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCG Clone Rv355 :::::::::::::Rv355SP6.seq::::::::::::: TTNACTGGCCTTTGGTCCACACTAGACAATACTCAAGCTTCCAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGG (SEQ ID NO. 472) TCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCCGCTGCCGGCAGGCCGATTAGGCGGGCAAATTAGCCC GCCGCGGCTCCCGGCTCCGANTACGGCGCCCCGAATGGCGTCACCGGCTGGTAACCACGCTTGCGCGCCTGGGCGGCG GCCTGCCGGATCAGGTGGTAAATGCCGACA :::::::::::::Rv355T7.seq::::::::::::: NGACGTCTTCCATCCGCGCGTCGTTTTGGCGGGTTGGCCACAGCAGCCCGCCGGTGACGGCGACGATGCTGGGCTGGT (SEQ ID NO. 473) TGCGGCCCTGCGCCACCGCGGCTTGCATGCTGGTTGGCTGTCTTGGGACGATCCCGAAATAGTCCACGCGGATCTGGT GATTTTGCGGGCTACCCGCGATTACCCCGCGCGGCTCGACGAGTTTTTGGCCTGGACTACCCGCGTGGCCAATCTGCT GAACTCGCGGCCGGTGGTGGCCTGGAATGTCGAGCGCCGTTACCTA Clone Rv356 :::::::::::::Rv356SP6.seq::::::::::::: CTTCCTCCTGAGTACCNCCCGTNTACTTTGGGATGGGTAAAAAGGCGAATCNCCGTTTGGTCACGAACGCCGGGAGGG (SEQ ID NO. 474) ACAATCTCGGGCGGCTGGGGCCTCTCGCGGGAANGCCCGAATGTACGGTGTCTCGACACTTCCCNTCCCCCTCCG :::::::::::::Rv356T7.seq::::::::::::: GAGCATCGGGACNTACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCNC (SEQ ID NO. 475) CGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCNT GATCNACATCGTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCNCAGCGGACGATCACNT CGCCGCGATCGCC Clone Rv357 :::::::::::::Rv357SP6.seq::::::::::::: TACTCATGANCATCCTTTAATCANNGCTTTGCGTTTTTTTATTAAATCTTGCAATTTACTGCAAAGCAACAACAAAAT (SEQ ID NO. 476) CGCAAAGTCATCAAAAAACCGCAAAGTTGTTTAAAATAAGAGCANCACTACAAAAGGAGATAAGAAGAGCACATACCT CAGTCACTTATTATCACTAGCGCTCGCCGCAGCCGTGTAACCGAGCATAGCGAGCGAACTGGCGAGGAAGCAAAGAAG AACTGTTCTGTCAGATAGCTCTTACGCNCA Clone Rv358 :::::::::::::Rv358SP6.seq::::::::::::: CTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANACNCTGCCAAGGCC (SEQ ID NO. 477) ACCGAATACAACAACGCCGCCGAGGCGTTCGCANCCCAGCTGGTGACCGCCGAGCANANCGTCAAAAACCTCAAGACG CTGCATGACCAGGCGCTTANCNCCGCANCTCAGGCCAAGAAGGCCGTCNAACGAAATGCGATGGTGCTGCACCANAAG ATCGCCGAGCGAACCAAGCTGCTCAGCCNG :::::::::::::Rv358T7.seq::::::::::::: CATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGANCCCCTGACACCCCGCT (SEQ ID NO. 478) AAGGCTGCTCAGCTCGGTGCATTACCTCACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGACGGCACCTG GTTGCGGGCGAACTTCATCATCAGCTTGGACGGCGGCGCTACCGTCGATGGCACCAGCGGGGCGATGGCCGGGCCCGG CGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCGTGGTCGGCGTGGGCACCGTGCGCATTGAGGG CTACTCCGGCGTCCGGATGGGTGTCGTCCAGCGCCAGCAC Clone Rv359 :::::::::::::Rv359SP6.seq::::::::::::: TACTCAAGCTTGCGGGTGATCGCCTTGGTCAACGGCACCGTGATCGGATCGGGGTCNACCGCACAAATGGACTGGAGC (SEQ ID NO. 479) TTCGGCGAANTCATCGCCTATGCCTCGCGGGGGGTGACGCTGACCCCGGGTGACNTGTTCGGCTCGGGCACGGTGCCC ACCTGCACGCTCGTCTATCACCTCNGGCCACCGGAATCATTCCCGGGCTGG :::::::::::::Rv359T7.seq::::::::::::: GTTGGNGCCTCGTCGGCGAACAGTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAG (SEQ ID NO. 480) GCGCTGACGTTCGCCGCGATTAGCTGTTTGATGGACGCGGTGGTGATGTTCTGATCACGGAACTGGCTGTAATAGCCC AGGGTCGCCACGCTTTCATCCGGGCCCGGACCCGGCGCACCGAGCGTGTCGCGCAGGTATGCGACGTGATTTTCGCTG AAGTCCCCGTACCCGGAGAACT Clone Rv35 :::::::::::::Rv35SP6.seq::::::::::::: TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACG (SEQ ID NO. 481) CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTCCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGG CCACCGCCGCCGGAGACGCTGCCTTTGTCACCGAATACAACAACGCCGCCGAGGCGTTCGCAGCCCAGCTGGTGACCG CCGAGCAGAGCGTCGAAGACCTCAAGACGCTGCATGACCAGGCGCTTAGCGCCGCAGCTCAGGCCAAGAATGCCGTCG AACGAAATGCGATGGTGCTGCGGCATAAGATCGCCGAGCGAACCAAGCTGCTCAGCCAGCTCGAGCAGGCGAAGATGC ACGAGCA :::::::::::::Rv35T7.seq::::::::::::: CAGGCATGCAAGCTTCGGAGGCAGACCCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACT (SEQ ID NO. 482) CTGGTCAGCTCGGAGCCGCTGACACCCCGCTAAGGCTGCTCAGCTCGGTGCATTACCTCACCGACGGCGAACTCCCCC AGCTTTACGACTATCCGGATGACGGCACCTGGTTGCGGGCGAACTTCATCAGCAGCTTGGACGGCGGCGCTACCGTCG ATGGCACCAGCGGGGCGATGGCCGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCG TGGTCGGCGTGGGCACCGTGCGCATTGAAGGCTACTCCGGCGTCCGGATGGGTGTCGTCCATCGCCA Clone Rv360 :::::::::::::Rv360SP6.seq::::::::::::: TACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACA (SEQ ID NO. 483) AGAAGGATTCGCTGGAGCGGTGGCTGTCCAAAATCACCCTCGCCCANACCTGCTACGGGCACTTCTACATCGAGCACA ACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCNAAACGTTGTGGGANTTCC TGCCCCGCANTGTTATCGGCGGCTTGCGCT :::::::::::::Rv360T7.seq::::::::::::: GGCCATCGCCACCGCNCCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTC (SEQ ID NO. 484) CTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACCGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGA GCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCGATCCATTCGAGGTCCGTCGCCGCGTCGG TCGAGTGGCGGTCACACTCCANGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAACAGA TCTTCTGTCCGACGACTACACCACCACCCAGGCCATCGC Clone Rv361 :::::::::::::Rv361SP6.seq::::::::::::: GCTTGCGGGTGATCGCCTTGGTCAACGGCACCGTGATCGGATCGGGGTCNACCGCNCAGATGGACTGGANCTTCGGCG (SEQ ID NO. 485) AANTCNTCGCCTATGCCTCGCGGGGGGTGACCCTGACCCCGGGTGACNTGTTCGGCTCGGGCACGGTGCCCACCTGCA CGCTCGTCAAGCACCTCNGGCCACCGGAATCATTCCCGGGCTGGCTGCACNACGGCGACNTGGTCNCCCTCCAGGTCG AAGGGCTGGGCNAAACAANGCAGACCGTCCGGACAANCGGCACTCCTTTTCCGTTGGCTCTTCGGCCGAATCCGGACG CCNAACCCGACCGGCG :::::::::::::Rv361T7.seq::::::::::::: GTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAGGCGCTGACGTTCGCCGCGATTA (SEQ ID NO. 486) GCTGTTTGATGGACGCGGTGGTGATGTMCTGATCACGGAACTGGCTGTAATANCCCAGGGTCGCCNCGCTTTCATCCG GGCCCGGACCCGGCGCACCGAGCGTGTCGCGCAGGTATGCGACGTGATTTTCGCTGAAGTCCCCGTACCCGGAGAACT CGAACACGCTGAGGCGCTCGTCACCGTCGTNNCGGCGACCAAGCGCGGCGAGCAACTGCGCAAAATCGTTAAGANAGG TCGAATCGTTGAAATTCGGCACCACCTGCACC Clone Rv363 :::::::::::::Rv363SP6.seq3::::::::::::: CACAAGACAATACTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANAC (SEQ ID NO. 487) GCTGCCAAGGCCACCGAATACAACAACGCCGCCGAGGCGTTCGCAGCCCAGCTGGTGACCGCCGAGCANANCGTCNAA AACCTCAAGACGCTGCATGACCAGGCGCTTANCGCCNCAGCTCAGGCCAAGAAGGCCGTCGAACGAAATGCGATGGTG CTGCAGCANAANATCGCCGANCGAACCAAGCTGCTCAGCCAGCTCGAGCAG :::::::::::::Rv363T7.seq::::::::::::: CCACCCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGAGCCGCTGA (SEQ ID NO. 488) CACCCCGCTAAGGCTGCTCAGCTCGGTGCATTACCTCACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGA CGGCACCTGGTTGCGGGCGAACTTCATCAGCAGCTTGGACGGCGGCGCTACCGTCGATGGCACCAGCGGGGCGATGGC CGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCC Clone Rv364 :::::::::::::Rv364SP6.seq::::::::::::: GCTTTCCGCCGATACCCNCCATGTCCCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAG (SEQ ID NO. 489) TGCGGATGATCGGGCCGCCTACGTCGTGGTGTACCTCGNCGGTAACAACGAAACCGAANCGTATGACTCNGTCCACGC GGTG :::::::::::::Rv364T7.seq::::::::::::: CAACCCGANTTGGCTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACAC (SEQ ID NO. 490) CTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGATCTACNACGCCGNGNGGAACGCTTCNGCCGC GGGCGTGACCGCNTCCCGTT Clone Rv365 :::::::::::::RV365SP6.seq::::::::::::: GGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCG (SEQ ID NO. 491) GGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGG TAGCACCCTGGCCGGGCGATGATCTGCCAGCGTCCCCGCGGGTAGTCGCCGCCCGGGCGG :::::::::::::Rv365T7.seq::::::::::::: CAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGC (SEQ ID NO. 492) GCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCC CACGGTT Clone Rv366 :::::::::::::Rv366SP6.seq::::::::::::: CTCAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTT (SEQ ID NO. 493) GA :::::::::::::Rv366T7.seq::::::::::::: TTGGTGCCCGGAATGGCGAGTCCCATTTANTCGCTGATTTGTTTGAACAGCGACGAAACCGGTGTTGAAAATGTCGCC (SEQ ID NO. 494) TGGGTCGGGGATTCCCTCTCCAAGCAAGAGTAACTGGCCCCAAATAAAGTTACTCGTCGTCTTGCAAAGACCGCTACC CGATGCCATTTATGTGTTTCCTTACGCTCNNNNTTCCGGTGCGCCATCATTATCTGCACCTTTGCACTGCACATTGAG CTTAGCAGCGCTCG Clone Rv367 :::::::::::::Rv367T7.seq::::::::::::: GAATTNGCTTTCGGCGCCATCGGCCCAGGACCGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGA (SEQ ID NO. 495) TGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCG TGACCGCATCCCGTTGACCGGGCGGATCGCNGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGC GTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATCGTGGCGAG ATTCGCCGGGTACGCCGATGAAGTGGTGT Clone Rv368 :::::::::::::Rv368SP6.seq::::::::::::: TAAAGCTTTCGTCAGTTCATNGNGCCCCCGGACCAACAAAAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGG (SEQ ID NO. 496) TGATTTCTTGGCCGCCGCTGACGGCGCNAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCAC GAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCNACATCGTCACCGCCGCACCACTGCCCGGCCTCGG GTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACNTCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCGC TGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAANACCATCNACCTCTGCAACAACGGCGACCCGATTTG TTCGGACGGCAACCGGTGGCGANCGCACCT :::::::::::::Rv368T7.seq::::::::::::: CCGGGAGGGACCATCNCGGGCGGCTNCGGCTTCTCTCCGGAAGGTTCTANNGTNNNGCGTTTCNACNCTTCCCGTCGC (SEQ ID NO. 497) CCTGCGACCGCCGAACATTCGGGGTATGGNNGCANCCTGTNAGCATCCNGGCCGGGC Clone Rv369 :::::::::::::Rv369SP6.seq::::::::::::: CTCAAGCTTCCGCATCAGATCGCTATAGAACCGGTGCGCGTCCCCACCGAGTGGCTGGTCGCCTTCCAGCACGATCGT (SEQ ID NO. 498) TACCGCGTTATCGGAATCAAACTCNCCGAACACCTGACCAACGCGCTTGATCGCCTGAATCGATGCGGCGTCGCTGGG GCTCATCGATACCGAGTGTGCTTTTCCGACCACTTCCAGTTGCGGTACGGCGAGATTGACAAAGGCGGTGAAGCCCAG CCAGAGCAGGACGATCACCNCCGCAAACCGGCGGATTTGCCCG :::::::::::::Rv369T7.seq::::::::::::: GCTTGGCAGCCTGCGGCTGGGCGCCCTNGAGCTCTTCGATCTGGATCTCCGGACTCGAGATGCTCACTTGCCCGGCCG (SEQ ID NO. 499) TGGACGTACCCATTGCGGCCGGGACCCCAGCGCCCCAGGTGACCAGCGAGTTGGGCTGCACGCTGACCGGCCCGTCGG GGTCGACGCCGGTAACGGTCAGCAGCTCCGANGTCCNNCTGATCCCGACCGCAGCTGCCAATGCGCGGCTGGCAGCCG ACGTGGATGTGCCGGGGCCTAGATCGCGGGGCAGCAGCGAGACCGCGTCACCGACGGTCATCACCTTGCCGAGTTTNG GCCTGCCGCAN Clone Rv36 :::::::::::::Rv36SP6.seq::::::::::::: GCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTNCACACAGGAAACAGCTATGACCATGATTACGC (SEQ ID NO. 500) CAAGCTATCTAGGTGACACTATAGAATACTCAAGCTTGAGCCATCGGGCTATCAGCTGGTTGATGTCCCG :::::::::::::Rv36T7.seq::::::::::::: CAGGCATGCAAGCTTGTCGTCTATCACATCCGACCACCAACCGCCCGACGGCTCGGCAGAACGCCTCCGCATATGGGT (SEQ ID NO. 501) CGACGACCAGCGGGTCGGACTTCTGGGCTGCCAGCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGAACCGACACTCC TTGTGATGTCCCACCTATCACCTTCGGTACGCACCCAATCGACCCTACGCGGCTAGCTCAGCCCCGATCTTCCAGAGC TCCGCCCG Clone Rv370 :::::::::::::Rv370SP6.seq::::::::::::: GCTTTTTGAGCGTCGCGCGGGGCCGCTTCCCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTCACCGA (SEQ ID NO. 502) AGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGA CGGCGACGCGGTAGGTGGTCAATTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTG :::::::::::::Rv370T7.seq::::::::::::: CGANCCTGTTCGACGGCTACCTGAATCACCCCGATNCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCGCACCG (SEQ ID NO. 503) GCGACGTCGCGGTGGTCGACGGCAGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTCGGGTG GATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCATCCGGACGTGGCGGAGGCGGCAGTCGTCGGGG T Clone Rv371 :::::::::::::Rv371SP6.seq::::::::::::: NAAGCTTTGTCACACCAAGTGTTTCNACCAGNCGCTCCATCCGGCGAAGTGGATACTCCCAGCAGGTAGCAGGTCGCC (SEQ ID NO. 504) ACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCG :::::::::::::Rv371T7.seq::::::::::::: CGCTGGNCGCCGGCGCTGGGCTGCGGTAACCAATTACCACAACACTTTTCGGTAGCCGAACAGCGGCGCGTACCAGCG (SEQ ID NO 505) AAATGGCACAGCCACCGCAGTCGCCGACATCCCGCGAAGATGTGGCAGATTTTCGTGCGGTCGAGCCGGCGAAGGCCT AGCGTCATTGTTGCCTGGCAAGGTTGCTGGGCCCGG Clone Rv373 :::::::::::::Rv373SP6.seq::::::::::::: CTCAAGCTTCTTCTGCCCCTTGCCGTTNCGGATNACATCCGGCAGCGACTCGGCTTCGGCGTCGATGTCGAAGTTCTC (SEQ ID NO. 506) GATCAGCTTCTGGATCGACTCCGCGCCCATGGCACCGGTGAAGTACTCGCCGTAGCGGTCGACNAGTTCGCGGTAGAG GTTTTCGTCNACNATCAGCTGCTTGGGCGCCANCTTGGTGAAAGTGCTCCAAATGTCCTCCAACCGGTCCAGCTCACG CTGCGCGCGGTCACGGATCTGGCGCATCTCGCGCTCGCCGCCGTCGCGAACTTGCGCCGCGCATCGGCCTTGGGGCCC :::::::::::::Rv373T7.seq::::::::::::: GTTCACACCTACCTACTATGCCNCAATTCNCCGACACGGGTGGCATCAACACGGGCGATAAGGTGGAAATCGCTGGGG (SEQ ID NO. 507) TGAACGTCGGGCTGGTGCGCTCGCTGGCAATCCGCGGCAACCGCGTGTTGATCGGATTCTCGTTCCCCGGCAAGACAA TCGGGATGCAAAGCCGGGCAGCAATTCNCNCCNACACCATTCTTGGCCGTAAGAACCTGGAGATCGAACCCCGCGGTT CGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCANACCACTACGCCATACCAAATC Clone Rv374 :::::::::::::Rv374SP6.seq::::::::::::: CTCAAGCTTTACGCCGACGCCGGCCTACACAACACCAAGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCG (SEQ ID NO. 508) CACACCTGGTTCGTGTTGCGGGAATTACTCGGACACCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGC TCCCTGGTGGGCGCCCCGATCGAGTTGGGAAGCTGATATGTGCTCTGGACCC :::::::::::::Rv374T7.seq::::::::::::: TCCCNCATGGGATAACGGGTTTAGATTTCNACAACGGCACCGTGTTTCTCAACAAGCCGGTCATCAGCTGGGCCGGCG (SEQ ID NO. 509) ACAACGGTATCTACTTCACCCGCTTTCGCCCGTACAAGAAAAACCACTAGGCCACCATCGAGTCCAAGAACAACCACC TGGTCCGCAAGTACGCGTTCTACTACCGCTATGACACCGCCGAGGAACGCGCCGTGCTCAACCGGATGTGGAAGCTGG TCAACGACCGCCTCAACTACCTCACCCCGACCATCAAACCGATC Clone Rv375 :::::::::::::Rv375SP6.seq::::::::::::: CTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCNCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTAC (SEQ ID NO. 510) CGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACAACGCTGATTGAAT CNAGTTCCANGTCCAGCGGGT :::::::::::::Rv375T7.seq::::::::::::: TNAACAGCTCGCGGCAGCCCACGACCTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAA (SEQ ID NO. 511) TGCGGCTCTGCTGGCCCGCAACGAANGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACA GGTTGGCCCACAACCGGCCGCTTGATGNNNNGTCGGCAAGCCCGGCAGTNGCCAAACCCAGCGTGATCANGCTCGGCT CGCGAGTTCGGCGAANAAGTGGCTCGCCTGATCACCTACCATCGGCCANGATCTGCGTGTCA Clone Rv376 :::::::::::::Rv376SP6.seq::::::::::::: GCCANCCGGCTTGGCGTCGACTCCCGTTCNGCACATCATACGGTCCCCGGTACTGTCCAACTGCGCCGGTGCGCTAGC (SEQ ID NO. 512) CAAACGTCACGACTCTCAGTGATCCCAGTTCGTGATCCGGCCGGTGGCCCCGCTGCGGCGGGGGCTNATNTACTTCGG ACTNATTATCTCATCCAAAGGACACCGGGCCGGTGGCTGGAATCCCATGGTGCGATCGGCCACACAN :::::::::::::Rv376T7.seq::::::::::::: CCGACCTGGTATCTTCCGATAGCGCGCGTTGATATCCGGTCTGATCTCCTGCCCTTAACGCCGGATCTCAGCAGGTCC (SEQ ID NO. 513) CCATGCAAAGATCCGAGGTGTCCCNGATCTAGGGGTCCTCGTCCTCCAGATGATGGAGCAAGTCGGCCC Clone Rv377 :::::::::::::Rv377SP6.seq::::::::::::: CTCAAGCTTCGGCTCAGGCGGCGCTGCCGGTAACGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGC (SEQ ID NO. 514) GGCTACGTGCCATCAAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGC :::::::::::::Rv377T7.seq::::::::::::: CATCACCTGNTTCATGAACTGGAAGCACCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGT (SEQ ID NO. 515) CGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGA CGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGG GTGT Clone Rv378 :::::::::::::Rv378SP6.seq::::::::::::: AGCTTAGCTTCCCGCCCCGGCAATAGGGCTCCAGCTCATCCGGTGTGACCAGATAGGGGCCCAGGGTGATACCGCTGT (SEQ ID NO. 516) CTTTGCCCTTGGCCTGTCCGATGCGCAGCTGGCCCTCCAGCATCTGCAGGTCCCGTGCGGACCAGTCGTTGAAAATGG TATAGCCGATGATCGACCG :::::::::::::Rv378T7.seq::::::::::::: CCNGAACAGAAGCGGNGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGCCTTTTTACTAACCGAACCCGATGTG (SEQ ID NO. 517) GGCTCCGATCCGGCGCGCATGGCATCGACGGCGACGCCGATCGATGACCGCCAGGCTTACCACCTT Clone Rv379 :::::::::::::Rv379SP6.seq::::::::::::: CTCAAGCTTGCGCGACTCGAGAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCA (SEQ ID NO. 518) CCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATGCTGCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAGA ACGCCACCGGTCCAGCGC :::::::::::::Rv379T7.seq::::::::::::: GCNAGGCGGTATAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTC (SEQ ID NO. 519) ACGCTGCTGCTGAGTGCGTGCCAGACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTG AAGTTGGTGTTCTCCATGGACTTGGGGATGT Clone Rv37 :::::::::::::Rv37SP6.seq::::::::::::: GTGTGGAACCGTGAGCGGATAACAATTTCACACAGGAAACAGCTNTGACCTTGATTACGCCAAGCTATTTAGGTGAGG (SEQ ID NO. 520) CTATATTAATACTCAAGATTGCGGTCGAGCACATCGGCCCAAGAACCGCCGAAGGCACGGCGGAACGCCTGCGGCACA TGGGGCGACGACCAGCGGGTCGGACTTCTGGGCTGTCCAGCCGGATCGCGCCGTCGCGA :::::::::::::Rv37T7.seq::::::::::::: CACTGTCAGTACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACC (SEQ ID NO. 521) CAAGCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACCAATACCAGATTGCTCACCAGGAACTCAC GCAGCACCGGGACGGATGTCAGCCACCACGCCCATCTGGGGTGGTAGCGGGGAAATACGGCTAACGCGGCTCCGGTGC CGGCAGCCCAGCGCAGACCCTCGGCGGCGGACACGGCAAACAACGACGACCCATAGTTGTTCTTTGCCGGATGGCCGT GTTTGCGGACATATCGGGCGGCGGCGCGGGCGCCGCCGAGGTAGTGGCTGAGGCCCATCTCGTGCCCGCCGAATGGCC CCAGCCAAACCGTGTA Clone Rv381 :::::::::::::Rv381SP6.seq::::::::::::: CTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCCGCCGCAACA (SEQ ID NO. 522) TGAGCCANCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAG CTTCCATATCCCGCGACNAACGAC :::::::::::::Rv381T7.seq::::::::::::: CTCAGAAGCCGCTAGCTGGTAGAGTCGCTGACCGGTGCACGTGGCGNCAATGTGCGCTGCCGGTTCGCG (SEQ ID NO. 523) Clone Rv382 :::::::::::::Rv382SP6.seq::::::::::::: CTCAAGCTTGCGCTCATCAAGCGCGAACAGCAGGGCGGTCGGCTGGTCGCCATGACGGGTGACGGGACCAATGACGCA (SEQ ID NO. 524) CCCGCGCTCGCGCAAGCCGATGTCGGGGTGGCNATNAATACCGGCACCCAGGCGGCCCGGGAAGCCGGCAACATGGTC NATCTCCACTCC :::::::::::::Rv382T7.seq::::::::::::: ACTTCTATTTCGACTGGTGTGCTGTGGCGCGATCCGACTGCCGGCGTGGTCAAGGCCGGCCAGTTGTGGGATNCCACA (SEQ ID NO. 525) GGCAC Clone Rv353 :::::::::::::Rv383SP6.seq::::::::::::: GCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGT (SEQ ID NO. 526) CATGGCGTCACCCTACCCAAGCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACCAATACCAGATT GCTCACCAGGAACTCAC :::::::::::::Rv383T7.seq::::::::::::: CGATATTCGTCGGCCGCGTTGTCTCGACTGGGTCGCGT (SEQ ID NO. 527) Clone Rv384 :::::::::::::Rv384SP6.seq::::::::::::: GACCTCGGCCACCAAGCCGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCACAT (SEQ ID NO. 528) GGTCCGCGGCACGGCCANCCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGCN :::::::::::::Rv384T7.seq::::::::::::: CCGGAAGTCTAGGGGACGACCTACTCAGCGCAAAATGTCGCTAATGTGAGTCCGCCCCACCAGGGCAGATCAACCCAT (SEQ ID NO. 529) GTCGATGATGACCTACCCGGATACCGGATTGGCGGT Clone Rv385 :::::::::::::Rv385SP6.seq::::::::::::: AGCTTCAGTTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATAC (SEQ ID NO. 530) CCCTTACTTCGGNAACGCTGGGCGGATTGGCCCTGCCGCTG :::::::::::::Rv385T7.seq::::::::::::: CCGCCTACGGGTCGAACATGCATCCCGAGACCGATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGG (SEQ ID NO. 531) TTACCCGGGTGGCGGCTGACC Clone Rv386 :::::::::::::Rv386SP6.seq::::::::::::: GCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATG CTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCNTTCGTNACCGGCAAT (SEQ ID NO. 532) ACCTACCGCAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGC ATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAN :::::::::::::Rv386T7.seq::::::::::::: GCCTTTCCGCACAATCTGTACCCCAGGACCNTCTAAAAAATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCG CTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAA CAAATTGACGCAGCGGTTCCGCTGACCANTNNGTGCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAAC CTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAG (SEQ ID NO. 533) GTGATTGTTAACCTGG Clone Rv387 :::::::::::::Rv387T7.seq::::::::::::: GCAGACCAACAAGATGCATCGGGATCATACGCCGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCCCAC (SEQ ID NO. 534) Clone Rv388 :::::::::::::Rv388SP6.seq::::::::::::: CTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCNGGACGCGACCGTCNAGGTGGCGATCCGGCTTGGCGTCCACCCG (SEQ ID NO. 535) CGTAAGGCANACCANATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAANACTGCCCGCGTCGCGGTATTC GCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCGGATGTTGTCGGGAGTGACAATCTGATCGANAGGATT CAGGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGACACCGGATCAGATGGCCAAAGTCGGTCNCATCGCTCGGGTG CTGGGTC :::::::::::::Rv388T7.seq::::::::::::: CCACGGCCTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGC (SEQ ID NO. 536) CGGATTCCACCACATCCCCTTGCGAAACTCCGTTGGGTGCAATGATGTACCGCTTCTCCCCATCGAGATAGTGGAGCA ACGCAATCCGTGCGGTACGGTTCGGGTCNTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATTGCGGC GAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTC Clone Rv389 :::::::::::::Rv389SP6.seq::::::::::::: GGCGGCTGCGTCGGCGAGATGATCGCCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCCAACGTGCTTTCCGGTCC (SEQ ID NO. 537) GGCGACCACCATGTCGCATGCGCCGAC :::::::::::::Rv389T7.seq::::::::::::: GCAATCGCCTTGGCGGTCGCCGGGTTGTCACCGGTGATCATCNCGGNGCGGATGCTCATNCGGCGCATTTCGTCNAAT (SEQ ID NO. 538) CGTTCCCGTATGCCCACCTTGACGATGTCCTTCATATGGACCACGCCGATGGCCCNCGCGCTNCTG Clone Rv38 :::::::::::::Rv38SP6.seq::::::::::::: CCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAG (SEQ ID NO. 539) CTATTTAGGTGACACTATAGAATACTCAAGCTTCCACATCGGTATGCCAAAGCATTGCGCCGCTATCGATTTCGCGCT GGGTCGCCAAGGTGGACTTCTTGCTCAGCGACGAGATCCCGTGGTCGGATCCGCCGGCTGCGGCGGGCTGCGACCCTG CATCTCGGCGGCACCCGTGACCAGATGGCGCGCGCCGAGGCAGACGTCGCGGCGGGACGCCACGCCGACTGGCCGATG GTGCTGGCCGCGTGTCCGCACGTCGCCGACCCCGGCCGCATCGACGAAACCGGCCGCCGTCCGTTCTGGACCTATGCC CACGTGCCGTCGGGGTCCACGCTCGACGCGACCGAGACCGT :::::::::::::Rv38T7.seq::::::::::::: CGCGTCCACCGCAGCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTGTTGGCGGCGTCGCCGTATTGTGCG (SEQ ID NO. 540) GGCCAGCCTTGTGCGGGGGCCGCTTCTACCCACGAGTCGGCACTTCCGCAACCGCCCAGCTCGACCGCGATTACGGCG GCCGCAACGGCCGCCGGAAGGCGTCTCGCAAGCGCCTTATCCTTTCGCAGGTTCCCAGATCCTTCCGCTACGTGGGTC GCTCATCGGCGGGCCCGGCCGAATGAGTACAGGTGAGGGTAACCGCTACAAATGAAGTTGGTCAGTGCTGGCCAACTG TGTAATGGTTGCCCGGCTCGGGTCACCACGTACATTCTGGCAAGGCGGGCGAGATTCGGTTCCTCGCGTCCTTGGCCG GTGGCGGTTCCCGGTTGTCCGTGGGCGTGTCGTGTACGTGGTGTAAGTGTCGTGAACTCCTCAGTTTGGGCT Clone Rv390 :::::::::::::Rv390SP6.seq::::::::::::: CTCAAGCTTGCGCTGGATCTGGCGGCTGAGCCTGTTCTTGGGCAACATGCCGAGGGATCGCCTTTTCCACCACGCGGT (SEQ ID NO. 541) CGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTGTGCAGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCT TGTGCTGCAGTTTGTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACNATGACNAAGTCACCGCCATCGACATTGG GGGCGAACGTCGGCTTGTGCTTGCCGCGCAGCAGGTTGGCCGCCGCGACGGCAAGGCGGCCAANCACCACGTC :::::::::::::Rv390T7.seq::::::::::::: TTTGGGATGGGCAAAAAGGCGAAGCNCCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTC (SEQ ID NO. 542) GCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACC TGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGC GATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCGCTGCGCCCGTG GACCGGTGGGTCTATCCCGGA Clone Rv391 :::::::::::::Rv391SP6.seq::::::::::::: CTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCANAGTCGGGGGCATCCGCACCGGCTCGCATCG (SEQ ID NO. 543) CATCATCCTCCCACGACGGGCCGCTCATCAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGGCCA CTGCNACAATTCAAACACGGTGTCACACGGTGAATANTGTCNANATGGGCTCTGATCAACCGTCNCAAACCCGGTTTC :::::::::::::Rv391T7.seq::::::::::::: GAATTCTGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCGA (SEQ ID NO. 544) ACCCGGATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTA CTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCGG GTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTTTC GCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCTGCACAACACCCCGGTGAGGTCGTTTTTGTTGCAGTTGGGC GGAATCCGTGCTCTGGGTTACAGCCCCGAACTCGTCNCGGCGGTGCGCGC Clone Rv392 :::::::::::::Rv392SP6.seq::::::::::::: GCAGTTGGGAATCGCTCTGCAGCAAACCANTATTCTGCGCGACGTTCGAGAGGACTNTTTGAATGGACGGATCTACCT (SEQ ID NO. 545) GCCGCGCGACGAGCTGGACCGATTAGGCGTACNCCTCCGCCTGGACGACTCCGGGGCACTCGATGACCCCGACGGACG GCTCGCGGCACTGCTGCGGTTCANTGCCNACCGCGCCGCANACTGGTATTCGCTGGGACTGCGGCTGATTCCACACCT CGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCNGCTCGCCTTGATCAGACCATCGCC GGCGGTCGTCTACCATCGGCGAATCTCTCTGTTCGGGACTGAANAANGCCCAAGTGGCGGCGGCAGCACTGGNCTCTT CGGTAACCTGCNGACCGCCCATTGGACCGCTACCG :::::::::::::Rv392T7.seq::::::::::::: TTGATCTGGACGTCTGAGACGGTGATCGGNCCGAACCTGAATTGTCCGGTAATGCCCAGCGCAGAAAGCANGGTGGTG (SEQ ID NO. 546) GCCGGGGCGGTGAANCCGGCGTCGGCGGCACCGTCGAAGTCGATGTGGATTGCCGGAATGGGGATGTCCGGCACGGCG AAGCCGTAGTTCGCTTGTCCCGTGAGGCCCANGTGGATGGGGGGAAGGATCGTGGTGTCCGGGATGATAATGGGGCCG ATGCCGCCGGTTGAAGTCCAGTGGATCGGGAATTCGGGAATCGTGATGCCGACGTTCAGGCCGAACAGGCCCTCCAAG TTGCCTCGCCACNAGATGCCGTTGCTGAAGTTGCCCGACATGAGGGCGCCGGTGTCCACATTGCCCGAATTGGCGACG CCGGTGTTGGC Clone Rv393 :::::::::::::Rv393SP6.seq::::::::::::: CACGTAGGCGCCGTCCATAAATNACTCCGCCGCGCTTCGCACATCCTCGTANCGATCCTTGGCGAGCAGGTCAACCGG (SEQ ID NO. 547) GCGCTGCCCGTCNAGGAGCCGGTTTTTGGCGTGCAGCCACTGGCCGACACCTCGGGGGGTAAGCGAATCCGAGAGCAG GAGGACNAGGTCACGAANCTGCGCCAGCCGGTCGTACCGCTCAGGGCGGATGTCGCCGGTCCGCCACCCGCGTACCGC CCGATCGGACACCTGTATGACCGCGGCGACNTCGACCTGGGTGACGCCGAAGGGTTTCAGGGCATCNACNATCTCGCT GGCCTCGACCGCCCGGTCCAGGGTGACCGCCATCGTGGTTCCTCCGCAACTTCCGGTTCTACTACCGTAAACGCTACC G :::::::::::::Rv393T7.seq::::::::::::: CGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTCGGACANCAAAACGTCAAGAACTACGACGGCAG (SEQ ID NO. 548) TNGGACAGAANACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGAAGCTGATATGTGCTCTGGACCCAAGCAAGGAC TGACATTGCCGGCCAGCGTCGACCTGGAAAAAGAAACGGTGATCACCGGCCGCGTAGTGGACGGTGACGGCCAGGCCG TGGGCGGCGCGTTTCGTGCGGCTGCTGGGACNCCTCCGACGAGTTCACCGCCGGGAGGTCGTGGCGTCGGCCACCGGG CGAATTTCCGGTTCTTCGCCGCGCCCCGGGATCCTGGGACCGCNGGCGCGCGCTGTT Clone Rv396 :::::::::::::Rv396SP6.seq::::::::::::: CTCAAGCTTTGTCCGACAAGCGTTCCCGGGCGGTCAGCAAGCGAACGTCGGTTGGCCCACTGCGGGTCGATATTGCCCG (SEQ ID NO. 549) CCAGGGA :::::::::::::Rv396T7.seq::::::::::::: CGTCAGCACGGCGACGTCGCGNTACGCCGAGCAGTTACACAATCGCTCTGCAGCAAACCAATATTCTGCGCGACGTTC (SEQ ID NO. 550) GAGAGGACTTCTTGATTGGACTG Clone Rv39 :::::::::::::Rv39SP6.seq::::::::::::: CTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTAC (SEQ ID NO. 551) GCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCGCGCAGCGGCGGGTTGACCCGGTTCACGCCGTCATAGC TGGCCAATCTGGCATCGTCGATCANCATGTGGTGGGGGGTGACCTCGGCGGTGATCGAAATACCCTGGTCCTTATCCC ATTTCAGGATTTCGACGGTGCCCGCGGCCGACGCGTGACAGATGTGCACCCGGGCGCCGGCGTCACGGGCCAGCAAGG CGTCGCGGGCGACGATCGATTCCTCGGCGGCCCGCGGCCATCCCGCCAGGCCCAGCCGCGCCGCCATGGGTCCCTCGT GCGCGACGGCGCCGACCGTCAGCCGGGGCTCCTCGGCGTGCTGGGCGATCAGCACGCCCAAACCGGTG :::::::::::::Rv39T7.seq::::::::::::: CCGACGCGCACTACGTGCTGGTGTCCACCCGCGACCCGCACCGGCACGAGCTACGCAGCTACCGCATCGTCGATGGCG (SEQ ID NO. 552) CTGTCACCGAGGAACCTGTCAATGTCGTCGAGCAGTACTGAACCGTTCCGAGAAAGGCCAGCATGAACGTCACCGTAT CCATTCCGACCATCCTGCGGCCCCACACCGGCGGCCAGAAGAGTGTCTCGGCCAGCGGCGATACCTTGGGTGCCGTCA TCAGCGACCTGGAGGCCAGCTATTCGGGCATTTCCGAGCGCCTGATGGACCCGTCTTCCCCAAGGTTGTTGCACCGCT TCGTGAACATCTACGTCAACGACGAAGACGTGCGGTTCTCCGGCGGCTTGGCCACCGCGATCGCTGACGGTGACTCGG TCACCATCCTCCCCGCCGTGGCCGGTGGGTGAGCGGACACATGACACGATACGACTCACTGTTGCATGCCTTG Clone Rv3 :::::::::::::Rv3SP6.seq::::::::::::: TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACG (SEQ ID NO. 553) CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCAAG GGGCGCCAACGCGGTGTCCGCGCCGTCGAGCTGAACGTTGCTGCCCGCCTGGAGAACCTGGCGCTGCTGCGCACCCTG GTCGGCGCCATCGGCACCTTCGAGGACCTGGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGANGTGTGC ACCCGGTTGATTCGCTCGGCCTTGCCGGATGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAAGACGAAGTTGTGGTG GAGGCTTCTGCTGCCTGCGACACCCACGACGTGGTGGCACGGGCAGCTTTAGCTGGCATTCCT :::::::::::::Rv3T7.seq::::::::::::: GGAAACACCGNCGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCCGTC (SEQ ID NO. 554) TTGGCCAATTGCCGCGGCACCAAGCCGTCGCGCGCCATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATC ACCACCGTGGTAAGCCCGGCCAGCGCGCCGACGGAGATGATGCCGCTGGCCCAGTACACCCCGTTGGCCTGGAACGCG GTGGCCAGATTTGCCGGCCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCGAT ACCGCGACGTAGAGAAGGGTCACGACCCCCAGCGACGCGAGAATCCCTCGAGGGACGTCTCGTTGAGGACGCTTGGTC TCCTCGGCCATGGTGGCCACGATGTCAAACCCGATAAACGCGAAGAACACGATCGATGCCCGGCCAGCACGCCCTA Clone Rv40 :::::::::::::Rv40SP6.seq::::::::::::: CCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA (SEQ ID NO. 555) CGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGTCCTCGGGCGTGGCCTCGGCCAAGAAATCGTCGACGC CGGCCTCCTGTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACCGGTGATCATCACGGTGCGGATGCTCATTCGGCGCA TTTCGTCGAAGCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGCCGATGGCCCGCGCGCTGCTGT TATCGGTCCATTCCGCAACGACTAGGGGTGTCCCCCCGCCGGAGCTGATGCCGTCGACAATGGCACCCACCTCCTCAG TGGGGTGGCCACCGTGATCGCAAAACCACTTCATCACCGCAGCCGCGGCACCTTGCGGATCCGAACGGATGCGCTC :::::::::::::Rv40T7.seq::::::::::::: TTCGTTCGATGGCGCCGCCCCGGCTACGGTTTGACCTGTGGGTGTCGAATTGGGGTCAAATTCCGAGGTCGGCGCGCT (SEQ ID NO. 556) AAGAGTGGTCATCCTGCACCGCCCGGGGGCCGAACTGCGCCGGCTCACACCGCGCAACACCGACCAGCTGCTGTTCGA CGGCCTGCCCTGGGTATCCCGCGCGCATGACGAGCACGACGAATTCGCCGAGCTGCTGGCTTCCCGCGGTGCGGAAGT GCTGTTGCTGTCGGACCTGTTGACTGAGGCACTACATCACAGCGGGGCCGCCCGCATGCAGGGGATCGCCGCTGCCGT CGACGCACCGCGGCTGGGACTGCCGCTGGCGCAAGAACTTTCGGCCTACCTGCGTATCTCGACCCAAGCANGTTGGCG CATGTGCTGACGCCGGCATGACTTCAACGAACTCCCNTCCGACACGCCGAACGAAGTGTCGTTGGTGTTGCGTATGC Clone Rv412 :::::::::::::Rv412SP6.seq::::::::::::: GCGGCGAGTGTGGTGGGTGCCGAACACGAATCCAACGACGCACTGGCGGAGAGATACCACTTGCTGTACTGGAAGCAC (SEQ ID NO. 557) GTGCTGATGATCTCCCGTGGAATGTGCCTCGCCCCCGTCTATCGAAAACAGTGAGCATGCTGCG :::::::::::::Rv412T7.seq::::::::::::: CAACCGCGCTCGGCGCGTCTGGGCCTTCCGCCGGCTCCGCCGACAATTCTATCTCTGGATCAGCGGGGCTCTCCGGGC (SEQ ID NO. 558) CGGCCTCCGCGAACTCAACAGGCCGCGCCTTCCGGCCGAAACATTCCCTAGCCATATATGATCGCACCTCGATACACG ATCTGGCGGCAACACCGCAAAGCGTCCGACGGGCCCAACCTCCGCAATTCAGGTATCCGGG Clone Rv413 :::::::::::::Rv413SP6.seq::::::::::::: GAAGGTCGGCGAAGGTGTGGCTGGMTGCCGATCACGAATCCAATGATGCAGTGGTCGGAAGATATTAGCCACTTGCTG (SEQ ID NO. 559) TTCTGGAGACAGGTGCTGATGATCTCCCGTGGAATGTCCCTCGACTCCGTCTATCGAAATCTGTGAACA :::::::::::::Rv413T7.seq::::::::::::: TCCTGCGCTCTGGGCCATTCTCGGGTCTGCCGACAATTCTATCTCTGGATCTGTGGGGCTCTCTTGGCCGGCCTCNGC (SEQ ID NO. 560) GATCTCTTCANGGCGCGCCTTCCGGCCGAAACATTCCCTATCCATATATGATCGCACCTCTATACACCGTTTGGCGGC AACACCGCAAAGTGTCTGTCG Clone Rv414 :::::::::::::Rv414SP6.seq::::::::::::: AGCTTTACGCTGGCGTATCAGCGTTGGGGCCGCTGCCATTTCGGTCGCCCAACGCGTTGCCAGCTCCCTGCGCTGTCA (SEQ ID NO. 561) GGGCTTGCGCGCCAAACTGGCCACCGCAACAAACTTGGCTGAGCTTGATC :::::::::::::Rv414T7.seq::::::::::::: CTCTATCTGGCGTCACATTCGCAATCTTTAGATTGCAGATATCGATAAAATCACCCGCGCGACAAGACCGCCATGTCA (SEQ ID NO. 562) TCCTTTCGATGTTATTTCGCCGGCCTGGGGAAAGCGCAACGACGTTGCCTACACGTTCCGCCGT Clone Rv415 :::::::::::::Rv415SP6.seq::::::::::::: AGCTTTNCCTTGCATCTGCACCCCGATCCACGTCAGCCACGTCGGCGTTCTCCACCAAGAAGTTGCGGGCATTCTCCT (SEQ ID NO. 563) TGCCCTGGCCGAGCTGCTCGCCCTCGTAGGTGAACCAGGCACCCGACTTGCGGATGAGGCCCTGATCCACACCCATGT CGATCAGCGAGCCCTCCCTGCTGATTCCCTTGCCGTAGAGGATGTCGAACTCGGCCTGCTTGAAGGGGGGCGAACAGT TGTGCACGACAACCCCTTCGGCGACGAGGGTGTGCAGTTCCTCGACCTCGAGGTCGAACGTTCGTGCCCGCCGCGTTG GCAGCACTTCTCGGATCACGGAATAGCGGANTTCTTCCGCCAGCATGTCGTGCAGGAATTTGTCATCCAGGGCATCCG CGAGCGCCTGCACGCG :::::::::::::Rv415T7.seq::::::::::::: ACTGTCNAGGGAATGCTTCGCAGCATCTACCTGCAGTCGCTTGTGCATAAGCGGACGGCCCNACCTGTTCGTGTTCCG (SEQ ID NO. 564) GGACACCAGACGCGGGAGCACCGGCAGTACGGCGAAGGTTTGAGCGGAAGGAGTTGCGCAAATCGGGGCGCGCCCAAC ACCCGTCCGCAAGACGCGGTCAACGACCTGTTTCAGGCGATCAGGGTCACCGACTCACCTGCACTGAGAACAAGCGAT CTGCTGATCTGCCAGAAGATGGACATGAATGTCCACGGCAAGCCTGATGGCCTGCCGCTCTTCCGGGAATGTTTGGC Clone Rv416 :::::::::::::Rv416SP6.seq::::::::::::: TGAATTATGATCCCGACACAACTGCATCANTTTAGCCGCGTCGNGATGCTATCCGCCGACGGTTTGGANCNGGTCCGT (SEQ ID NO. 565) GTCGTTCGTGTTGATCTCACCCGAAGTTGTGTCCGCCGCCGCCGGGGATCTAGCGAACGTGGGATCGACAATCAGCGC CGCCAACAAGGCGGCAGCGGCTGCGACCACGCAGGTGCTGGCCGCGGGCGCCGATNAGGTGTCAGCGCGCATCGCGGC GCTGTTTGGTATGTACGGCCTGNAATATCCGGCGATCAGTGCGCAAGTTGCCGCGTATCACCANCAGTCCGTGCAG :::::::::::::Rv416T7.seq::::::::::::: AACGGGGACCNCAAGAAACCATTCAANAACGAGGGGTCGTCACCAACGTCGAAACCGACGGTTGCCAGCCGGCCCACG (SEQ ID NO. 566) ATATTGCGTGCTCGAGGGTCCGCTGTACCCTCACCGAACGTGAGTCCCACACCGCGGAGGCGGGCGACTCTGGCGTCG TTAGCAGCCGAGCTCAAGGTGTCCCGCACCACTGTCTCGAATGCTTTTAACCGACCGGATCAGCTCTCCGCCGATCTA CGTGAACGAGTGCTTGCCACGGCCAAGCGACTGGGCTATGCCGGACCGGATCCGGTGGCGCGATCGTTGCGGACCCGC AAAGCCGGTGCGGT Clone Rv417 :::::::::::::Rv417SP6.seq::::::::::::: AGCTTTGGAGCCNCNCCGANCCNCCGGTACGCCCCGCCACCGCCGTACCCGGCACCCGACCCCTTTGAGCCGTTCGCC (SEQ ID NO. 567) GTGGCCGCGGTGGANCTGGCCGACGAGGGACTGATCGTGCTGGGCAAAGTGGTCGATGGCACGCTGGCCGCCGATCTG AAGGTCGGCATGGAGATGGAGCTGACGACCATGCCGCTGTTCGCCGACNACGACGGTGTGCAGCGCATCGTCTACGCG TGGCGGATCCCATCGCGCGCCGGCGACNATGCANAGCGCANCGATGCTGAGGAGCGGCGCCGATGAGGATGAGCGCGC CGGAACCCGTTTACNTCCTGGGTGCCGGTATGCACCCGTGGGGGAAATGGGGTAATGACTTC :::::::::::::Rv417T7.seq::::::::::::: TTCTCNCATCGTTCGTACTNNGATGGGACGCTGCTGCCCGAGGCGATCCTGGCCAACCGGCTCTCGCCGGCGCTGACC (SEQ ID NO. 568) TTCGGCGGGGCGAACCTGAACTTCTTTCCGATGGGCGCTTGGGCCAAACGTACCGGGGCTATCTTCATTCGGCGTCAG ACGAAAGATATTCCCGTCTACCGCTTCGTATTACGTGCTTACGCCGCGCAGCTGGTGCAAAACCATGTCAACCTCACC TGGTCGATCGAAGGGGGTCGGACCAGAACGGGCAAGCTACGGCCACCGGTGTTCGGGATCCTGCGTTACATCACCGAT GCGGTCGACGAAATCGACGGTCCCGAAGTGTATTTGGTGCCGACCTCGATCGTGTACGAACAGCTGCACGAAGTGGAA GCCATGACCACCGAAGCCTATGGCGCCGTGAA Clone Rv418 :::::::::::::Rv418SP6.seq::::::::::::: TTCTTCCGGGTACCGCTGATCGGCGGCACCATCACGCACCCGGTGCAGGGCGAGGCGGCCGCCGGTGTGGTGTTGCTA (SEQ ID NO. 569) CGGCCGGCCAGCCCGGGTACCGGTGTGATCGCCGGTGGTGCGGCCCGCGCGGTGCTGGAATGTGCGGGGGTGCACGAC ATCTTGGCCAAGTCGCTGGGCAGTGACAACGCGATCAATGTGGTGCACGCCACCGTGGCCGCGCTCAAGCTGCTGCAC CGTCCGGAGGAGGTGGCGGCGCGCCGCGGTTTGCCAATAGAAGACGTCCCCCCGGCCGGGATGCTG :::::::::::::Rv418T7.seq::::::::::::: GTCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAACGCT (SEQ ID NO. 570) TGCAGTGCCGCCGAATAGGCGGCTACGTCGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCTGGGCGGCG CCGACGTCGACCGTGGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCGATCGTCGACCCGCACGGCAGATCGGCGCGA TGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTTGCGAAACGCCCCCGCCGGGTGCCTTCCGTCGG TTGGCTTTACCGCAAATTTGGGGTTGCCCCT Clone Rv419 :::::::::::::Rv419SP6.seq::::::::::::: AAAGCCACGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTC (SEQ ID NO. 571) GGACACCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGA AACTGATATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCTACCTGGAAAAA :::::::::::::Rv419T7.seq::::::::::::: TTTCGCCACCGCNAGGTCGTGCGCGTTCCAGAAAAGCGTGGTTTCGCCGGGCGCGAGGATTCGACGGTCCAACTGACC (SEQ ID NO. 572) AGCCGGTCCCGCCACCCGTTAGGCAGGATCGCGGTGTCTATATGTTCGCCCTCGGCATAAACGCCATTGCTGCGGTGA AAATCGGACATCTCGCCGATTGCCACGTCTACATGATCCGCTTTGTCCCGCGCCGGGTCGTTGACAAACGCGATGTCN GCCTCCTGGGAAGCGGTGGC Clone Rv41 :::::::::::::Rv41SP6.seq::::::::::::: TCGCCAAGTGGATTCGTGCTCACCNACGAGATCCGTGGTCGGATCCGCNGCTGCGGCGGGCTGCGACCCTGCATCTCG (SEQ ID NO. 573) GCGGCACCCGTGACCAAATGGCGCGCGCCGAAGCAGACGTCTCGGCGGGACGCCACGCCGACTGGCCGATGGTGCTGG CCGCGTGTCCGCNCGTCNCCGACCCCGGCCGCATCNACCAAACCGGCCGCCGTCCGTTCTGGACCTATCCCACGTGCC NTCGGGGTCCACGCTCGACGCGACCGANAACGTAACCAGCGTCCTCGANCGGTTCGCCCCCGGCTTCCGTGACATCGT GGTGGCGGCCGCGCCGT :::::::::::::Rv41T7.seq::::::::::::: GTACCGTCACCATGATCGCCCCCATCCGCATCGGTGAGCTGATAGATCCCAGCCGGTTTCGCCAACCCCGGAGCGATC (SEQ ID NO. 574) TTGGCGCGCTGCTNGTNGTCNCTGANACNTAGCCACCAACAGAGCCCGGTGTGCGACAAGANGACTGATCGGATCTCT CCGGACACNTCGAGGGGGTCWTCAGGAGNCCGGGCGCCACCCCGAGGTAAGCCTCCGCCCAGCCTCACACCGCGACCG GGTATCNCAAGTCGCGCAATAANCCCACCACCTCCTCGGACCCCACGTTGTATGCGGCTGGGT Clone Rv42 :::::::::::::Rv42SP6.seq::::::::::::: ATACTCAAGCTTAGACCTCACTGATGTGGCGGGACGCGGGAGATAACCGCGGTTCGAGCCGTTCAACAGTGGTGGTTC (SEQ ID NO. 575) CCACACCAGTTGTTTGCCTTTGCGAAGTAAAGCGATTCGATTTGCTCGAAAAGAGGGCTGGCTGCTCGTGAGGGACAT CCATGGCCGATACCTCAGCGATCTCAACGGTCAAGCGACTGCATGTTTGGCGCAAGGTATCGCTAAGCATAGGTTCGT GACGGATTTGACAGCAAGAGCTTTCCAAAGATTGCTGTCCACATANTGATTCGCATCTCTACACCTCTTCGCCGGTGC TGTCAAGAGCCATTCGAATCAGTTATCTCGCTCGTGCTTGGAANAAATTTTCCCAGCCTGCGTTGGACAAACCGCGTC GCCAAAGCGGT :::::::::::::Rv42T7.seq::::::::::::: AGCTTCCCGAGAAACAGTGCATTCCCTAAGCAGCCCGTTGTCACGCCGATGAGTGAAGAGTGCACGCAATCGCCGGAA (SEQ ID NO. 576) TCCGGCAAAGCCCTGCACAAGCGAAATCAACCCGGAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGA CAAACGGCAGAAGGCGGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAGCGCGCATCGGCATCGGTGCCGC CACGGTGGAGACGACGTCCGCGGGCGTCTGGGTCAGTAACCCGCCGACCAGTTCTCGGGCAAGCTGGTCGACCATCGG GCGCCACGTCTCCAACGCGCCACGCGCCATACCTGGTGCCAGTTGCTTGCGCATCCGGGTGTGCGCCGGCGGATCGGA CGTCGCAGAAACGCAGCCACCCCGTGAGAAGTGACCCACGGCGCTGGACACGTGTCTGGTTAC Clone Rv43 :::::::::::::Rv43SP6.seq::::::::::::: CGGCCGGGATGTGCGCAATGGCAGGTTGTCGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCTTG (SEQ ID NO. 577) CGAAAGTCCGTTGGGTGCAATGATGTANCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTT CGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATGGCGGCGAAAGTCGATCATCCGGTAAGC GCGCTTATGACCGCCGCCTTTGTGCCNGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGCGACCGTGCAGCGGGCG CACCAGCGACNTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCGT GGGCTTGTACTTGCGAATTGCCATGGTCTAATCAGGTCTTTCTCTCACCTCTCGTCGCCGGGCTAGGGCGCATTGCCT GCTCCT :::::::::::::Rv43T7.seq::::::::::::: TAGCGGTGTAACCAACTCCCGGGTCACCACCCGCAAACCTCTTGCGGCAACAGCACCGTCGACGCGTCAACCGGGCTG (SEQ ID NO. 578) CCCGGAATCCTGTGGATGGGCATCGAGTGCATGGTCACGACGTCCCCGACGCGGCCGGTGGCAACGACAAGTGGCCCG GATGCACCACAAATGACGGCCGCACACCGGTGGGGACGGCCAGCACGAGAGCCGTGTCGCCGAAGTCGACGCTAATGC CGTAGGCATTGGCCGTCACAACAGGCGACGCCCCGCGTACCACCGAGTCCACGGNGGTTGGGCGGTCTCCTCGGCCAA CCAGGCGTGAACCCGGCGGATCCGAATGCAGCAAGACCCGTGGGC Clone Rv44 :::::::::::::Rv44-2ndSP6.seq::::::::::::: CCATTGGTCGGTGTGCGCATACCANTACNACGCGCCGGGCACCTGACGCGGCGGCCGCAACCATTCGGTGGCCATCGC (SEQ ID NO. 579) CATCGTCTGCCACCCGGTCAACGGACGCACCTTCTCCTGGCCGACCTAGTGCGCCCACCCGCCGCCGTTGCGTCCCAT CGATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATCTGCTGTGGAACCAGTGACANATTCCGCCGC CCATGATGATCNTCGACCGTCCTCCGGATTCGGTC :::::::::::::Rv44-2ndT7.seq::::::::::::: GCCGGCCTGGTCAAAGGGGCGTCCGAAGGANCCGGGCTGGGTAAGAAGTTCCTGGCTCATATCCGCGAATGCGACGCC (SEQ ID NO. 580) ATTTGTCAGGTGGTGCGGGTGTTCGTCGACGACNACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATT GAGGTCGTCGAGACCGAGCTGATCCTGGCAGATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAANGAAGCN CGCACCAACAAGGCGCGCAAGCCGGTCTACGACCCGGC Clone Rv45 :::::::::::::Rv45SP6.seq::::::::::::: GATCCACTGACCACGATGACATATCGAAATGCTCGACGATTCCGATGGCGATCAAGGCCACGATGCCCTGGCCGTTGG (SEQ ID NO. 581) GCGGTATCTGGTGGATGGTGTACCCGCGGTAGGTTCCCGTGATCGTGTCGACCCAGTCCACGCGATGGGCGGCGAGGT CGTCGGCACGCATCACCCCGCCGTNTGCCGCCGAGTGCGCCTCGAGTTTGGCGGCCAGCTCTCCCCGGTAGAACTCTC ACCGTTGGTCGCCGCGATCTTCTCTANCGTCGCCGCGTGGTCAGGAAAGGTAAACAGCTCACCGGGTTTCGGCGCTCG TCCGCCGGGCATGAACGCATCTGCGAATCCGGGCTGGGATGCGAACAACGGACCTGTGCCG :::::::::::::Rv45T7.seq::::::::::::: TCTACTGCCGAATCGGGGAACGGTCCTCGCCCACCNGGTTCGTGTTGCCGGAATTACTCAGGACACCGAAACGTCGAG (SEQ ID NO. 582) AACTACGAGCGGAGTTGGACANAATACCGCTCCCNGGTGGGCGCCCCCATCGANTTGGGAAGCNGAAATCTGCTCTGG ACCCCACCCAAGAATGACATTGCCGGCCGCCCTCCAACTGGAAATAGAAACNGTGATCACCCGCCGCGTTCTTGGAAG GAATGGCATGCCCTGGGCCGGGCGTTCCTTCCGCTGCCGGACTCCTCCCACCAATTCACCGCCGAAGGCGTCCCGTCT GC Clone Rv46 :::::::::::::Rv46SP6.seq::::::::::::: ATACTCAAGCTTCTGTCACCGAAATCCCGCATGGGATAACGGGTTTAGATTTCGACAACGGGACCGTGTTTCTCAACA (SEQ ID NO. 583) AGCCGGTCATCAGCTGGGCCGGCGACAACGGTATCTACTTCACCCGCTTTCGCCCGT :::::::::::::Rv46T7.seq::::::::::::: CTGGCTCAAGCGCTCGGCGCGCAGGTGAACTCGGACCGGCTCGACGTCGCCGAACGCGAGGCGGTGCTGGCCCACGCC (SEQ ID NO. 584) GACGCCGTCGTCGCACATATCGGCACCGTGCACAAGTCTACAACAACGCCGGCATCGCGTACAACGGCAACGTCGACA AGTCGGAGTTCAAGGACATCGAGCGCATCATCGACGTCGACTTCTGGGGCGTCCTCCACGGGCCC Clone Rv47 :::::::::::::Rv47SP6.seq::::::::::::: CCGCCCTCCGCATTATGGGTCAAGAACCATCGGGTCGGACTTCTGGGCTTCCAACGCTCGCGCCGTCCCN (SEQ ID NO. 585) :::::::::::::Rv47T7.seq::::::::::::: CCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACC (SEQ ID NO. 586) CTACCCAAGCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACCAATACCAGATTGCTCACCAGGAA CTCACGCAGCACCGGGACGGATGTCGGCCACCACGCCCATCTGGGGTGGTAGCGGGGAAATACCGCTAACGCGGCTCC GGTGCCG Clone Rv48 :::::::::::::Rv48SP6.seq::::::::::::: TACTCAAGCTTGTCCAAATATCGAAGCGTCGGGTCGCGAGGCTCGGTCGGCAGCTCCAGCAAAACCCGCTCCACCCCT (SEQ ID NO. 587) AGATGCCGGTATCCCTCAAGGTCTTTATCCGCCGCTTCACCCCACTGGCACACGGTCACCGGCACGTCGCCCCCGGCC ATGGCGCGCAACCGCTGAAGCGGACCCGACAGCCGCTGCGGTGATGGACTGATCGCGATCCACCCGGCATTGAGCCGG GCTATCCGCGGGAAGTTCGCCGGTCCCCCGCCCACATACAGCGGAGGATAGGGCTTTGTCACCGGCTTCGGCCAGCAG TAGATCGGATCGAAGTCCACATATGTCCCATGGAATTCCGCCTGCTCCTGCGTTCAGATCTCGATTATCGCGCGCAAC CGCTCATCGATCACACGTCCGCGCACCGCAGGGTCCACACCATGGTTGGCGACTTCTTCGCGCAACCAGCCACACCCA CGCCGAAACGAAACCGTCCCTGCG :::::::::::::Rv48T7.seq::::::::::::: CAGGCATGCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTGCTCCACGGCACGTT (SEQ ID NO. 588) GATGGCACCAGGAATGTGTCCGGGCCGCTGGCTTTGTTCCTGCGGCAGGTGCGCGGGGGCCAGGATCTTGCCGGAGAA CTCGTCGGGAGAGCGCACGTCGATGAGGTTCTTGACGTTGATGGCCGCCAGGACCTCGTCGCGGAATGCCCGAATCGT GTTATCCGGCGGGGANGCGGTGTAGGAAGTCACCGGCCGGCTGACCGGGTCGCTGGACAGCGGGCGTCCGTCGAGCTC C Clone Rv49 :::::::::::::Rv49SP6.seq::::::::::::: ATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCC (SEQ ID NO. 589) CGCACCGCCGGCATCTCCCGGTCACGCAGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCA ATGATGCTGACCTGATCGGCCACCCGGGCGGTCTCGGCGTCGTCCCGTTCACTAATCGCGGTGCTCAGCAGCGTCTCG ACAGCCACCACCCGAGTGGAGACCAGATGCNCCACCACGGACCGCAGCGATGCCAGTCACCTCACCCGTCC :::::::::::::Rv49T7.seq::::::::::::: CAGGCATGCAAGCTTTGCAGTTGCTGACTAATGTCGGCCAACGTCACCACAATCGCGATGAATTCAATCATGCCGCCC (SEQ ID NO. 590) AGGGCGGCCAACCCAATGGTGGCCGCGAGCGGCAGCTCGATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACG AACAGGGTGAGGTCATAGGCGCGCAGGATAGTGACGAAGGCAAGACCTAGATCTGCCGTCGGAAGAAGAATCGAGTAT CCGGTCGACACAACGGAAGCGAAAGTGTCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGCGGCGGTAGC ACCGTCCGCACATACCGCGGGAACGCGGGCATCCGAATTTGGGGCAGGGTGTTCAAGGCGGCTGGCAACTCACCATGA ATCT Clone Rv4 :::::::::::::Rv4SP6.seq::::::::::::: CCGGCTCGTATGTTGTGTGGAATTGTGACCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAG (SEQ ID NO. 591) CTATTTAGGTGACACTATAGAATACTCAAGCTTGGCCGCAGGGCCGAGTCGATTGGTCGCGGTCGCCTCGACAGTTAG CTTATGCAATGCTAACTTCGGGGCAAAGTTCAGGCGGATCGGCCGATGCCGGGCGTAGGTGAAGGAGACAGCGGAGGC GTGGAGCGTGATGACATTGGCATGGTGGCCGCTTCCCCCGTCGCGTCTCGGGTAAATGGCAAGGTAGACGCTGACGTC GTCGGTCGATTTGCCACCTGCTGCCGTGCCCTGGGCATCGCGGTTTACCAGCGTAAACGTCCGCCGGACCTGGCTGCC GCCCGGTCTGGTTTCGCCGCGCTGACCCGCGTCGCCCATGACAGTGCGACCCTGNACCGGGCTGGCC :::::::::::::Rv4T7.seq::::::::::::: GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTC (SEQ ID NO. 592) CCGCGTGTCGGCAGGGCACACGCTCGGGGCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGGAGAGCAGTGCTCAGTCCC CCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGCTGACGGTCGCTGAGCAGCGCTC GCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGAGGCGCATTCGTGTTCGGCCGCCTGCGGGTCGGCCCCATCGT CGACGCGATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGAGTAAGTGTGCG TGGGCACGCGAGCCGGGTGCTGTGGTACACCCACCGTTGCATGAACAA Clone Rv50 :::::::::::::Rv50SP6.seq::::::::::::: ATACTCAAGCTTCACCAGGCGCCGGCGGGCCGCGGCGCCAAGCCAGGCAGCCGCGCTCGGCGCGTCGGGGCCTTCCGC (SEQ ID NO. 593) CGGCTCGGCCGACAGTTCGATCTCTGGATCGGCGGGGCTCTCCGGGCCGGCCTCGGCGACCTCAGCGGGCCGCGCCTT CCGGCCGAACCATTCCCTAGCCATAGATAACCGCACCTCAATGCACGGTTTGGCGGCAACCCGG :::::::::::::Rv50T7.seq::::::::::::: AGCTTCCGTCACGACCCGCCCTCGCCGGTGCCGGCGCCATCGGTCATCGGATCTCATGACGACGTCACGTAGGCCCGC (SEQ ID NO. 594) TAGCCGCGAGCGGGCGCGGTCAACTGGCGAGGCGGCGGCGACGTGACTGAGCTGGCCGAGCTGGACCGGTTCACCGCG GAACTACCGTTCTCGCTCGACGACTTTCAGCAGCGGGCTTGCAGCGCGCTGGAACGCGGCCACGGTGTTGCTGGTGTG CGCGCCGACCGGCGCTGGCAAGACGGTGGTCG Clone Rv51 :::::::::::::Rv51SP6.seq::::::::::::: ATACTCAAGCTTGCCGGGACCGCGGAACAGAACCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGCCTCC (SEQ ID NO. 595) CGACTAACCGAACCCGATGTGGGCTCC :::::::::::::Rv51T7.seq::::::::::::: ACGTTGGCTCTGCCGGAACGTATTTCCAGCGGCACGCATTCGGCGTGGGTGCCGGGCGCCGAGTTGCGTCGCTGGGAT (SEQ ID NO. 596) CACGCAGCAGTCGCCGGCGGCTGCCGTCGGGCTATGAATTGCACCGAGCCGGAAAATCCNCAC Clone Rv52 :::::::::::::Rv52SP6.seq::::::::::::: ATACTCAAGCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCG (SEQ ID NO. 597) CCGGCGGTCATGGCGTCACCCTACCCAAGCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTCTGAGCACCAAT ACCAGATTGCTCACCAGGAACTCACGCAGCACCGGGACGGATGTCAGCCACCACCCCCATCTGGGGTGGTAGCGGGGA :::::::::::::Rv52T7.seq::::::::::::: CGTTGGTAGCCCGATATGCATAGTGTATCTTACTGAACATGATTTCCATTATGGAGCCCGGGGTGCCGGCAGCGCGAA (SEQ ID NO. 598) CGGTGCGCCGTCAGACGCGGGCGGCACTGACCAGGGTGTTGCGGGCGAACATCGGCCCGGCTTCGGATTCCGGTCCGG GTACCGGGCGACCCACCGCTTCGAGGTA Clone Rv53 :::::::::::::Rv53SP6.seq::::::::::::: ATACTCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTCCTCCACGGCACGTTGAT (SEQ ID NO. 599) GGCACCAGGAATGTGTCCGGGCCGCTGGCTTTGTTCCTGCGGCAGGTGCGCGGGGGCCATGATCTTGCCGGAAAACTC GTCGGGAGAGCGCACGTCGATGAGGTTCTTGACGTTGATGGCCGCCAGGACCTCGTCGCGGAATGCCCGAATCGTGTT ATCCGGCGGGGAGGCGGTGTATGAGGTCACCGGCCGGCTGACCGGGTCGCTGGACACCGGGCGTCCGTCCAGCTCCCA CTTCTTGCGGGCGCCGTCCAACNACTTGACTTCTCCTGG :::::::::::::Rv53T7.seq::::::::::::: ATATCTTAAGCGTCGGGTCCCGAGGCTCGGTCGGCAGCTCCAGCAAAACCCGCTCCACCCCTAGATGCCGGTATCCCT (SEQ ID NO. 600) CAAGGTCTTTAGCCGCCGCTTCACCCCACTGGCACACGGTCACCGGCACGTCGCCCCCGGCCATGGCGCGCAACCGCT GAAGCGGACCCGACAGCCGCTGCGGTGATGGACTGATCGCGATCCACCCGGCATTGAGCCGGGCTATCCGCGGGAAGT TCGCCGGTCCCCCGCCCACATACAGCGGAGGATAGGGCTTTGTCACCGGCTTCGGCCAGCAGTAGATCGGATCGAAGT CCACATATGTCCCATGGAATTCCGCCTGCTCCTGCGTCCAGATCTCGATTATCGCGCGCAACCGCTCATCGATCACAC GTCCGCGCACCGCAGGGTCCACACCATGGTTGGCGACTTCTTCGCGCA Clone Rv54 :::::::::::::Rv54SP6.seq::::::::::::: ATACTCAAGCTTGTCGCGGTAAACCCGCAGCAGGGCGGTGGGTGCGGTGTCAAAAACAACCACACTTCTTTGCGGTTC (SEQ ID NO. 601) GGTGATCTCGACACCGGCCGCGAGCCGACCACCATGCGCGCGTAAATCGATCAGATCAGCGTCGGCTATCGCCTGGGT GCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCAGCATCAGTCCGGCGCCGGCCGACACCAGTGA CGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAGCGACCGCCAGGC AGGGGTGCCCTGGGCCAGCATCCGCAGCCCGAGACGCAGGACCGAGCCCAGTGCAGTAGGCAAAGACCGCTTGTCGGA GACATGAACTCCACGACCGT :::::::::::::Rv54T7.seq::::::::::::: AGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCAACACGAACGGC (SEQ ID NO. 602) CGGACGACGTGGGCCAGGGTCGCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCG TTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCT CGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAATGCAATCCCCCAAAATGCAATGCGTCGAGCTATTTCTCACAC CGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTT GTAATCGTTTGGAAATTCATCCTCATATGCCTCGATCGCTTCATAGGGTCCAGGCCAAACCGGGCA Clone Rv55 :::::::::::::Rv55SP6.seq::::::::::::: CTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCC (SEQ ID NO. 603) AAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGGCCACCTCGCGGTGTGTGGTGGAACCCATCTGAGCAGTGTG CCAAACCGGGGCAGACAGCTCCCAATTGACGTGAGCCCGCTCACTTGCTGGGTAAGCGTCG :::::::::::::Rv55T7.seq::::::::::::: TAGCGCCCCCTCCCGGGCGGAGCTCCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGC (SEQ ID NO. 604) CCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCTTGCGAAAGTCCGTTGGGTGCAATGATGTAGCGCT TCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGT TGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGG TAATCCGGCCATGCGCGTTGCGTCCACCGCGACCGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTGA TCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAAGCGTCGTGGGCTTGTTCTTGCGAATTGCATGTCTAATCA GGTCTTTCTC Clone Rv56 :::::::::::::Rv56SP6.seq::::::::::::: TGAAACTATATAATACTCAAGCTTGCCAAAGAAGACCTCGTCGACCAAGCAGGACGCGACCGTCGAGGTGGCGATCCG (SEQ ID NO. 605) GCTTGGCGTCGACCCGCGTAAGGCAAACCAGATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGC CCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCGGATGTTGTCGGGAGTGACGA TCTGATCGAAAGGATTCAGGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGACACCGGATCAGATGGCCAAAGTCGG TCGCATCGCTCGGGTGCTGGGTCCGCGCGGCCTGATGCCCAACCCGAAAACCGGCACCGTCACCGCCGACGTCGCCAA GGCCGTCGCGGACATCAAGGGCGGCAAGATCAACTTCCGGGTTGACAAGCAGGCCAACCTGCACTTCTC :::::::::::::Rv56T7.seq::::::::::::: GCTGAGCTCCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGC (SEQ ID NO. 606) GTTAGCGCCGGATTCCACCACATCCCCTTGCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATA GTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTC ATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGC GTTGCGTCCACCGCGACCGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGA TACGCTGGCGCCGCGACGACCAGGCGTCGTGGGCTTGTACTTGCGAATTGCCATGTCTAATCAGGTCTTTCTCT Clone Rv57 :::::::::::::Rv57SP6.seq::::::::::::: ATACTCAAGCTTGTTGGTGACCTCGCCGGCGAACAGTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTG (SEQ ID NO. 667) GGTATGCGGGAAGGCGCTGACGTTCGCCGCGATTAGCTGTTTGATGGACGCGGCGGTGATGTCCTGATCACGGAACTG GCTGTAATAGCCCAGGGTCGCCACGCTTCCATCCGGGCCCGGACCCGGC :::::::::::::Rv57T7.seq::::::::::::: GATGATCGCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCGAACGTGCTTTCCGGTCCGGCGACCACCATGTCGCA (SEQ ID NO. 608) CGCACCGACCAGGCCGAACCCGCCGGCCCGCACATGCCCGTTGATGGCGCCGACCACCGGCAGCGGCGACTCGACGAT GGCGCGCAACAGCGCCGTCATTTCCCGCGCCCGCGCCACCGCCATCCGGTACGGATCACCACCACCTCCGCCGGCCTC GCTGAGGTCC Clone Rv58 :::::::::::::Rv58SP6.seq::::::::::::: ATACTCAAGCTTGCCGCAATCGAAACCAACCTGTTTGTGCCGCAAGAAATTACGCCGTGGCCCGGCGCCGATCAAGAA (SEQ ID NO. 609) ACGCCCCGGCGCGCGGCGGTGTCGTCGTATGGCATGACGGGCACCAATGTGCACGCCATTGTCGAGCAGGCACCGGTG CCAGCCCCCGAATCCGGTGCACCAGGCGACACCCCGGCCACACCCGGTATCGACGGCGCGCTGCTGTTCGCGCTGTCG GCCAGCTCGCAGGACGCGCTGCGGCAAACCGCCGCGCGGCTGGCCGATTGGGTCT :::::::::::::Rv58T7.seq::::::::::::: TTGGCGGGTTGGCCACANCANCCCGCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGCCACCGCGGCTTG (SEQ ID NO. 610) CATGCTGGTTGGCTGTCTTGGGACGATCCCGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCCGCGATTAC CCCGCGCGGCTCGACGAGTTTTTGGCCTGGACTACCCGCGTGGCCAATCTGCTGAACTCGCGGCCGGTGGTGGCCTGG AATGTCCANCGCCGTTCACCTACGTGACCTTGATGGGATCCGGGGGNT Clone Rv59 :::::::::::::Rv59SP6.seq::::::::::::: NCGTGGACACCGGTGTCGANCGCCACCAGCCGCATGTCTGCANGTCNATTCCGTCCTCGGCAACATCTTGAATGCCGA (SEQ ID NO. 611) GCAGCGCCTGGGCGTGATCGGCAACCGGGGATGACCGCTCGCCGATCCGCTCGACAATCCCGGCGGCACGTGACATGC GCCGCCACGGCTCGACGAGCTGGAACTTCAGCGACGACGATCCGGAATTGATCACCAGCACGGTGCTACTCATGGACC CCTGCGCCTGAATCCCGTGATGGCCACGGTGTTGACTATTCGTCGACAGTGCACCCGAGATAGTCTTCACGGCTGCGT :::::::::::::Rv59T7.seq::::::::::::: CATGTATTGCCGTGCTCACGGCGCCACGCTCGATGGTTTCTCGAAGTCTCCGGGCTGGTGTACAGCTTCTCGTTGATC (SEQ ID NO. 612) TCGTTCGCCACGCCGTCCTCTTCCCGCCGACGACCCGATCTCGATCTCCANAATGATCTTGGCGGCCGCCGCCGCCTT GAGCAGCTCCTGGGCGATGGCCAGGTTCTCATCGATGGGCACTGCCGACCGTCCCACATGTGCGACGGAACAAAGATG TCACCTTGCTCACGCGTGCGCNAGATCNCANAAGGGCCGGACATACTGTCNACTTGTCCTTGGGCAGTGGTCCGTGTC AGCCCACGTGACGGGTACTTGGCGCGATAACGTGGTG Clone Rv5 :::::::::::::Rv5SP6.seq::::::::::::: GCCACCACGACCCGGCCGTAACTCTGCTCACGGAAATGCGGCCAGGCCGCGCGTAGCACGTGGTATCCGCCATAAAGG (SEQ ID NO. 613) TGCACCTTAAGCACGGCGTCCCAATTCTCGAACGACATCTTGTGGAAGGTGCCGTCGCGCAAGATCCCGGCGTTGCTC ACCACACCGTGCACGGCGCCGAATTCGTCAAGCGCGGTCTTGATGATGTTCGCTGCGCCGTCCTCGGTGGCGACGCTG TCCTTAGTTGGCGACCGCCCGGCCCCCCTTGTCGCGAATCTCGGCGACGACCTCATCGGCCATCGCCGAACGGCGCCC GTGCCCGTCGCGGGCGCCACCGAGGTCGTTGACCACGA :::::::::::::Rv5T7.seq::::::::::::: CAGGCATGCAACCTTTGTCCACACGGCGTCTACTCCGTGCAAGGTCCGACCGCTTCCACGTCCCGCCGTGACGGTGCT (SEQ ID NO. 614) CCATCTCCCTCAGCAACGCGTGAAGTGGTCCGATCCCGCGGCTTCAGG Clone Rv60 :::::::::::::Rv60SP6.seq::::::::::::: GTTGAGACGCAACCAGCGCACAACGACGATTTGGCGTAGCGGCGGACGTCTGCTCGATTCGATCACGTCGCGCTCGCA (SEQ ID NO. 615) TCGAGCATGGCCCGCGACGCTACACGATCGCCGTCGTCGATGACACGACCGAGCCGTACGCCGGCCGTAAGCCGCGCC AGGATTCGGCGAAAAACGTCTACGTGGCGGGTGTACTGGGTGTCGAATGATTCGTGGGGTGCGTATGCGTCCTGCAAT CGTCGACATAGATCCGTCGCCGCATCGCGTCGACAACTCCGGGTGAGTGGAATACACTTGCCGATCACGCGACGTGCG CGGATCGATGCCGACCGAAATACGACCACATGGCTCTTGTTGCNCAGTGTTGGCGGCATCAAATACCCTCAGTGCCGT CCGAC :::::::::::::Rv60T7.seq::::::::::::: TTNCCGCCTTNACGCCTACTCCNAGACGATGCTCGACGCGTGTGAGCACACGGCGCTGCTGTAGACGGCACGGCGCAG (SEQ ID NO. 616) CTGGATCGCGCTTGGTGCACCCAAGCCTCTACGCGCGTCGCTGCGTCGTCATCGGGTACCGAACATATTCCGGTCGTT GCGCAGAGTGTGCATGTGCGGCTCTTGTGAACGAACATAGCAAAGCGTATATGTCTGTGGCGGCTCTGCAGATATCGC GATAATACGTATATACATAAGGTGGCGCGCGATCTATCGGTATATCCGTTATGGCGGACGTGCGTGAGCGTGAGTCGC GGCGCATCGCGCACTTCGCGATCGCGTGACTGGTCCTCGCGACTGCGCGCATGCGTAGC Clone Rv61 :::::::::::::Rv61SP6.seq::::::::::::: GGTGATGACGCACTTGCTTCGAATGAGTCATTGACTACTCCCGTGGTTGTCCTGCGATGGTGGAGTGCCGCGCAGCCT (SEQ ID NO. 617) TGCCCGANGTCGCGATCGCGTCGCGGGCTTCGGGGAGCAGACTGACCTGCAGATGGAAGTCGTGCCACATGCCCGCGA ACGGCGAGCTCGATGCTTGTTTTCGAAGNGCGCANGCGGTTTCGATCTTGTCCGCGTCAACGCAGATCGGATCTCGCC GCGGTCTGCATGACGATGGGCGCAGGCCCGCTCATGTCCCGTAGACGGGGAGATACGGGCAGCCGCGGATCGAGACCT ACGTAGCGCGGCGCCCATCGTGCCATCGACGAAGAATGACGGATCGCGCAGCGCCGTCGCGTCGCTTCGATGTCACGC GAGATCGCCACGGCAGATCAGCGATGCGCGGGC :::::::::::::Rv61T7.seq::::::::::::: CGGTACGCCGGCAACAAACGCCTTGTGACGAGCGCGTCCGAGCGGTCATCGGCCTCCACCGTCATGCACAGCTCCTTC (SEQ ID NO. 618) TCCAGGTCTACGCCGACGTCGCGGTCCACATTGGTGAGCTTGGCGAATGCCTCGGCAACCTCGTCGAAATGCGCCTCC GCGTCCGCATCGAAGGTCGCCATGTCAAAGATCAACTCGACGTAGTAGCTAGTTACCGCATCAGGTCAGTGTTTGCTG GCCTCGGAGTCCGGCCGAACAATGGCCATTTCCCGCGACTCTAGAATCCAGTCATCGTCTCGGTGACGACGCCTTGCC GATCACATAGCTCGACCGGATCGGAGAGAATCTGGTTCTCGT Clone Rv62 :::::::::::::Rv62SP6.seq::::::::::::: ATACTCAAGCTTAAGCGCAGCAGTACCGGCGGTGCCTGGGCATCCCAGCAAAACGGGGAGCTCAACGAACGATTCCTG (SEQ ID NO. 619) AACGAAGGGTCGTCCACCAACCTCCAAACCGAACGGTTGCCAGCCCCGGC :::::::::::::Rv62T7.seq::::::::::::: GCAAGTCCGCTCAATGTGGTTGTGATCACANGACTACGTCGCCTCAATCAGCTCAAACGTCACCCCGTGGCGTGCTGC (SEQ ID NO. 620) GCAGCATGAAGGTCGGCGCCCGCACGATGTGGGCGAAGCAACAGGTAATAACTGGTCGGCATGGGTCAACCCTCATTG GGCCGTTGCGGATCGGGTGCACGCCCGGAGTGCCGGTCGAACTCAACACCGCCTTCACCGATCTTTTCGTCGAAAATG GCGGTCGTGTCGGGGTATACGTCCGCGATCCCACGAGGCGGAATCCGCTGAGCCGCACTGA Clone Rv63 :::::::::::::Rv63SP6.seq::::::::::::: ATACTCAAGCTTCGCGCCCTCAAGCGGCTGAAGGTGGTTCCGGCGTNCCAACNGTCGGGCAACTCGCCGATGGGCATG (SEQ ID NO. 621) GTGCTCGACNCCGTCCCGGTGATCCCGCCGGAGCTGCGCCCGATGGTGCAGCTCGACGGCGGCCGGTTCGCCNCGTCC GACTTGAACGACCTGTACCGCAGGGTGATCAACCGCMACMMCMMGMTGAAAAGGCTGATCGATCTGGGTGCGCCGGAA ATCATCGTCAACAACNAGAANCGGATGCTGCNGGAATCCGTGGACGCGCTGTTCGACAATGGCCGCCGCGGCCGGCCC GTCACCGGGCCGGGCAACCGTCCGCTCAAGTCGCTTTCCGATCTGCTCA :::::::::::::Rv63T7.seq::::::::::::: TGCGCATGGCAGTTGTTGCCGGCTTGAGTCGCGTTAGCGCGGATTCCACCACATCCCTTGCGAAGTCGTGGGTGCAAT (SEQ ID NO. 622) GATGTAGCGCTTCTCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGTACGTTGGGTCGTACTCGAGTGCGCANCTT GGCGTTGACACCATCTTTGTCATTGCGGCGAAGTCGATCATCCGGTAAGCGCGCTTATCGACGCCGCCTCTGTGCCGG GTGGTAATCCGGCCATGCGCTTGCGTCCACCGCGACGTGCAGCGGGCGCACACCGACTTCTCCGGGTGACGGGTGATC TCGGCGAATCAGAACCTGGCGCGCGACACAGCGTCGTGGCTGTACTTGC Clone Rv64 :::::::::::::Rv64SP6.seq::::::::::::: TGGGTGATCAGATACTGGCTAGTTGGTCGGGTGGGGTGATCGAAGATCGCGGTGGCCGGCAGCGTTACTGCGGTGACG (SEQ ID NO. 623) CTGTTAAGCGGTTACGTACTCCACGGCACTCAANGAATTANATCCCGAATCGGCAAACCCTGGCCAGCGTCGAGTCCG CAGCGCCGTCGCGCCCCCCACCGCTGCGGCATGCTCACATACCACCTCGATCGCTGCGGGAGTTGCTCGTCGGCCGAC CGACCGGCCAGCCGGGCGGCAAACCGGAGGACCCAAGATTCAGCACCACCATCGCTAGCCCGATCTGGCCGCGCGTGG :::::::::::::Rv64T7.seq::::::::::::: TCGTAGCGGTTGCGACCANTCCGCGGACAGCTCCGCCACGCGACGGGTCGGGATCACCGCGGTCAAACCACCGAGCGG (SEQ ID NO. 624) CGAGGATCTCTGGCCGTCGACGTGACCGCGCACGGCCGCGGTGATGGCCAGTCCCGACCGCCCTTCCACTTGGCGTAC GCGCTGGATGTGTTGTGCCGCAACGGAATCCCACCTCAATTATGACCTCGTTGTGGGCGAGCGCGGTATCGTACGCCC GACCAGGAATCGTCGATGCTATCTCACGTCACCGAAGGCCTCTCCCAGCACACCGCATCCAGAACGTGCACACNGTCG ACATGTCTCGGCGGATCCGCCTGCAGAACGAACGCCANGTGCGCTGTGCGACACGGGTCGCGATCACCGCTCGCACGC GGAGATCGGCACACGCGCAGCGCATCGATCATAATCTCTCGATGCGGTCTCCACCACCGAACAG Clone Rv65 :::::::::::::Rv65SP6.seq::::::::::::: ATACTCAAGCTTCGCTGAGGTGGTGGGGCACGATCACGTCACCGCACCGCTGTCGGTGGCGCTGGATGCCGGCCGGAT (SEQ ID NO. 625) CAACCACGCGTACCTGTTCTCTGGGCCGCGTGGCTGCGGAAAGACGTCGTCAGCGCGTATCCTGGCNCGGTCGTTGAA CTGTGCGCAGGGCCCTACCGCCAACCCGTGCGGGGTCTGCGAATCCTGCGTTTCGTTGGCGCCCAACGCCCCCGGCAG CATCGACGTGGTAGAGCTGGATGCCGCCAGCCACGGCGGCGTGGACGACACCCGCGAGCTGCGGGACCGCGCGTTCTA TGCGCCGGTCCACTCACGGTACCGGGTATTTATCGTCGACGAGGCGCACATGGT :::::::::::::Rv65T7.seq::::::::::::: GCACTCACGCTGGTACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAATAA (SEQ ID NO. 626) CTCGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCT GCTGGCCGGCATGGTGCAATCGAGCAGCACGCTCAACCCGTACACCAACCCCGACGGCGCGCTGGCCCGGCGGAACGT GGTCCTCGACACCATGATCGAGAACCTTCCCGGGGAGGCGGAGGCGTTGCGTGCCGCCAAGGCCGATCCGCTGGGGGT ACTGCCGCAGCCCAATGAGTTGCCGCGCGGCTGCATCGCGGCCGGCGACCG Clone Rv66 :::::::::::::Rv66SP6.seq::::::::::::: ATACTCAAGCTTGTATAAAAAGATCGGTGAGCGCATCGATTCGCTCCGCCGGGTTTGCCGCTGCGGCGGCGGAGCTGC (SEQ ID NO. 627) CGTGACCGTCTATTTGGGTGATCAGATACTGGGCTAGTTCGGTCGGGGTGGGGTGATCGAAGATCGCGGTGGCCGGCA GCGTTACTGCGGTGACGGCTGTTAAGCGGTTACGTACCTCCACGGCACTCAAGGAATTAAATCCCGAATCGGCAAACG CCTGGCCAGCGTCGAATCCGGCAGCGCCGTCGCGCCCCAGCACCGCTGCGGCATGCTCACATACCACCTCCATCGCTG CGGCGAATTGCTCGTCGGCCGACCGACCGGCCAGCCGGGCGGCAAACCCGGAAGA :::::::::::::Rv66T7.seq::::::::::::: CCTCATCATATGCCGATAGAGCTCTACATATTCAGGAGATCACCATGGCTCGTGCGGTCGGGATCGACTCGGGACCAC (SEQ ID NO. 628) CAACTCCGTCGTCTCGGTTCTGGAANGTGGCGACCNGGTCGTCGTCGCCAACTCCGGAGGGCTCCAGGACCACCCGTC AATTGTCGCGTTCGCCCGCAACGGTGAGGTGCTGGTCNGCCAGCCCGCCAAGAACAGGCAGTGACCAACGTCGATCGC ACCGTGCGCTCGGTCAAGCGACCATGGGCAGCGACTGGTCCATAGAGATTGACGCAAGAAATACACGCCCGGAGATCT CGCCGCATTCTGATGAACTGAACGCGACCCGAGGCTACTCGGTGANGACATNACGACGCGTTATCACACCCCGCCTNC TTCAATGACCCCACGTCNGGCACCAAGGACCCGGCAATCGCGGCTCACTTGNGCGATNGTCNACAACCAACGCGNCGC CTGGCTACGGGCTCAACAAGGCANAAGACACAATCCGCTCTCGATTGGTG Clone Rv67 :::::::::::::Rv67SP6.seq::::::::::::: ATACTCAAGCTTATCGAGGCGGCGCATACCGAAGCGTGGGAAATCCAGACCGAATACCGCGACGTGCTGGACACTTTG (SEQ ID NO. 629) GCCGGCGAGCTGCTGGAAAAGGAGACCCTGCACCGACCCGAGCTGGAAAGCATCTTCGCTGACGTCGAAAAGCGGCCG CGGCTCACCATGTTCGACAACTTCGGTGGCCGGATCCCGTCGGACAAACCGCCCATCAAGACACCCGGCGAGCTCGCG ATCGAACGCGGCGAACCTTGGCCCCAGCCGGTCCCCGAGCCGGCGTTCAAGGCGGCGATTGCGCATGCTACCCAAGCC GCTGAGGCCGCCCGGTCCGACCCGGCCAAACCGGGCACGGCGCCAACGGTTCGCCCGCCGGCACCACCGGTCCGGTGA CCGCAGTACGGTCCCCCCAGCCTGACTACCGTGCCCCGGCGGGCT :::::::::::::Rv67T7.seq::::::::::::: TGGCCGGGCTGGTAGCCCGCGTATGGCAAGGTTCCGCTCAATGTGGTTGTGATGCAGCAGGACTACGTTCGCCTCAAT (SEQ ID NO. 630) CAGCTCAAACGTCACCCCCGTGGCGTGCTGCGCAGCATGAAGGTCGGCGCCCGCACCATGTGGGCGAAGGCAACAGGT AAGAACCTGGTCGGCATGGGTCGAGCCCTCATTGGGCCGTTGCGGATCGGGTTGCAGCGCGCCGGAGTGCCGGTCGAA CTCAACACCGCCTTCACCGATCTTTTCGTCGAAAATGGCGTCGTGTCCGGGGTATACGTCCGCGATTCCCACGAGGCG GAATCCGCTGAGCCGCAGCTGATCCGGGCTCGCCGCGGCGTGATCCTGGCCTGTGGTGGTTTCGAGCATAACGAGCAG ATGCGAAT Clone Rv68 :::::::::::::Rv68SP6.seq::::::::::::: GTCCAGTCAAGCATCGGTCCTCTCCGACTACGCCAAGANTGGCGACGTGTCAGTGCANACAGCGGANATGGTGGCGCC (SEQ ID NO. 631) TATGCGTCGACGCTCACAAACNGCGGTGANCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCAGCCCGGCCGCGTGCC GTCAGCCGCATCCACTGGATGCCTTCTCGGNGTTTCAATCANGTACANGCGACGTTCGCCACCATCGTGCCGGGGCAC GGTTAGCGAGAAACCGCCGACTTCACCGATTGCCTCGGTGATGCCGTCGAACAGATCGGGCCTATTGTCGACAGCCAG TGTGATNCGTATTTGCCGCCGTGCTCCTCGTCGCAACGATGCGAACACAGATCCGTGGNGGACGATAGCGGCTGACAA NGTGGGGGCAACACAATCACATGCCACATTTCTTCATTTCACGCCCACAACCCAGACTTCGTCTCGATGNGCCG :::::::::::::Rv68T7.seq::::::::::::: CACGCGGTCTGGCCCGATCCGAAGATCCCTTTGCCGGCGTGGCGGCTCTGCTCGGCGGTGTTGTACACTTCTCGAACA (SEQ ID NO. 632) CCTCGGCACCGACACCACCACCGTNGCTTGAACACCGCCAACATCGGCAGCAGATCTTGATGGTCCTGGTGAATCCCA CGGTGACTTTGGAGTGGAAGGCGCCATACTGATCGCCGCGCCAGCACATGAGCTAGCGGCAGGAAAACCAGCAGCCGC TCACCTTGCGCAGCAGCGTCNGGTGATATGCCTGGCGCCCTTAATCTCGTGAACCAGTTGGATTGGGTCAACTGGCAG CCTTGGGTCTCCGGTGGTGCCGANGTGTANATAAGCTCCCGGGTCCGTCAACGTANTGCGCAGGCGGCGGTTACTCGG CGGGTCAACGAGCCCCGCTCGTGAGCNATCAGCCTTTGGACCGAACGGGATTCATACTCCGCAGGCGGCCCTCCGAAA TCGGCACATGTCCTTTGATCGTTCGCAACAN Clone Rv69 :::::::::::::Rv69T7D3.seq::::::::::::: GGCCATGTCACATCGGTGGTACAGGTAAACCGCGCCGTGTGCGCGGTCTCGGAGATCAGAACGTGGTCGCAGTTGAAC (SEQ ID NO. 633) CGCGGGCTTTCAGCCAGTCGCGATAATCGGCGGAAGTCGGCGCCTGCCGCCCCAACTAGCGCGACTCGCCACCTAGCA CACCGATGGCGAAGGCCATGTNTCCGGCCACGCCGCCGCGGTGCATCACCAAGTCATCGACTAGGAAGCTAAGCGACA NCTTGTGCAGGTGTTCGGGCAGTAGCTGCTCGGAAAATCGGCTGGAAACCGCATCAAATGGTCGGTCCAATCGAACCG GTTACCCGATCGTCACAAAAATCTCCGTCCT Clone Rv6 :::::::::::::Rv6SP6.seq::::::::::::: GGGTCTACAACCACCGGGTCTGACTTCTGGGCTTCCACCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGAACCGACA (SEQ ID NO. 634) CTCGTTGTGATGTCCCAGCTATCACCTCCGGTAGGCACCCAATCGACCCTACCCGGCTATCTCACCCCCGATCTCCAG GCTCCGCCGATCCATGCGCATCCCGGTCCGGATCCC :::::::::::::Rv6T7.seq::::::::::::: CAGGCATGCAAGCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTCCGCTCGGCATCG (SEQ ID NO. 635) TCGCCGGCGGTCATGGCGTCACCCTACCCAAGCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACC AATACCAGATTGCTCACCAGGAACTCACGCAGCACCGGGACGGATGTCAGCCACCACGCCCATCTGGGGTGGTAGCGG GGAAATACGGCTAACGCGGCTCCGGTGCCGGCAGCCCAGCGCAGACCCTCGGCGGCGGACACGGCTAACAACGACGAC CCATAGTTGTTCTTTGCCGGATGGCCGTGTTTGCTGACATATCGGGCGCGGCGCCGGCGCCGCC Clone Rv70 :::::::::::::Rv70SP6D2.seq::::::::::::: NCTACGCTGCTGAATGTTGTGCGCCGGAGGANCTCAAGACCCACGCGGTTGTACGCGGACNTGCGACATGTTCAACCG (SEQ ID NO. 636) CCGGA :::::::::::::Rv70T7D3.seq::::::::::::: CTAACCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGCCACAACGGGAAGATCGCCTTGAGCGTCGC (SEQ ID NO. 637) TCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTACTGATGCCGATCATGTCGACGTGTCCGTCGCATCAGCGTGCAG CGGCGACCCCTCGACGAGCCTCGGTGCCGCCGCGGCCAGGGCACCAGCTGTTTTAGCGCATTGTGCTCCGCCGGTAAT AAAGGANGTCGGTCGCCTCCGCTGCTGTGGTTGCGGAATAACATCTTCCCTTCCTGCAACAGGATGAGAATGGTTTTA ATTGCTC Clone Rv71 :::::::::::::Rv71SP6.seq::::::::::::: CTAAGCTTTCGGGTCCGCCGCCACTAGTACCGCGTTGCCGGCCCCGCCGACCTAGAATGTTCCGCCCATTGCCGTTTC (SEQ ID NO. 638) CTCCCGCCGCCGGGTT :::::::::::::Rv71T7.seq::::::::::::: TCTGGTGCCGGGTGTGCCGACGGGTCCGTCCGCCTCTGCTTCAGTGATTCTGTGATGCGACCGGCAACGTCCTCCTTG (SEQ ID NO. 639) TTCGGTGTCTATGTGGTCCGTCTCTCCTTGTTCCGCATACGATT Clone Rv72 :::::::::::::Rv72SP6D2.seq::::::::::::: GCGATCGNTNACCACAAGGGCGCAACCGTTCGCGCGTCGACTGAACGTGCTGCCGCCTGGAGAACTGGCGCTGCTGCC (SEQ ID NO. 640) ACCTGGTCGGCGCATCGGCACTTCGAGGACTGGATTTCGACGCGTGGCCCGACCTGANGTNGGCGGTGGACNNGTGTG CACCCGGTTGATTCCTCGGCCTTGCCGGGATGCCACCTGCGCCTGGTGGTCGAT :::::::::::::Rv72T7D3.seq::::::::::::: CGTGACCGGACGGGCTGCCGCGCGAACCGGTCTTGGCCAATTGCCGGGGACTGGGGCTGGAGTATAAAGCGGGCCTGT (SEQ ID NO. 641) TGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACG ACCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACTGGCGCTGGAAACCGCCGACGCA CATTAACCATAACCACCTGCGCGGCTGGTGCAAATGATTGAACTGGCACGNCCACTCACGCCAATATCCGCCAGAACA TCACTATTGCGCTGGG Clone Rv73 :::::::::::::Rv73SP6.seq::::::::::::: ATACTCAAGCTTCTTACCCANAGCATGAACCCCGCCGTCCAATGCCGCCACCGTGGTGCTGTCGGCCGGCCGGGTGCG (SEQ ID NO. 642) GGCACAATCGCCGAGTTCGGCGAACAGATCCTCGAAGGTCTTCACGGCCAGCGATTGTTGCACGTGTCAGCCAGCCAA GTCACGGTGGTTTGACGCCACACGTTCGCCACCGCCGCGCCGCGCATTAGGGCATCCTAATATAGGTTAGGCTACCCT ANTTATTCCTGTGGTCNAAGGAGGCAGCCGAACGTGACCTTCCCGATGTGGTTCGCAGTTCCGCCGGAAGTGCCGTCA GCATGGCTGTCCACCGGCATGGGCCCCGGTCCGCTGCTGGCCGCGGCCAGGGCGTGGCACGCGCTGGCCGCGCAATAC ACCGAAATTGCAACGGAACTCGCAAGCGTGCTCGCTGCGGTGCAGGCAACTCGTGGCAGGGGCCCAGCGCCGACGGTT CGTCNTCCCCATCAACCGTTCCGTATTGGCTAACCACCTGCACGGTGGCACCGCACAACGCCGCCACAAACGCGCCCC GGTATAC :::::::::::::Rv73T7.seq::::::::::::: GGCCGAACTTAATCGGTTGTTGGCGGCTGCCGAGTTGGGTCACTCGGGGGGTGTGCACTGGCACATGGTGGGCCGGAT (SEQ ID NO. 643) TCAACGCAACAAAGCCGGGTCGCTGGCTCGCTGGGCGCACACCGCTCACTCGGTGGACAGCTCGCGGTTGGTGACCGC GCTGGATCGGGCGGTTGTTCCGGCGCTGGCCGAACACCGTCGTGGCGAGCGGCTGCGGGTTTACGTCCAGGTCAGCCT CGACGGTGACGGATCCCGGGGCGGCGTCGACAGCACGACGCCCGGCGCCGTAGACCGGATTTGCGCGCAGGTGCAGGA GTCAGAGGGCCTCGAACTGGTCGGGTTGATGGGCATTCCGCCGCTGGATTGGGACCCGACGAAGCCTTTGACCGGCTG CAATCGGAGCACAACCGGGTGCGTGCGATGTTCCCGCACGCGATCGGTCTGTCGCGGGCATGTCCAACAACTTGAAAT CCCGTCAACATGGTCGAC Clone Rv74 :::::::::::::Rv74SP6.seq::::::::::::: GCTTCCCCTGATACTCGACCAGCCCCACTCGGGCCAATACGTGAATGTCCTAGCATTTTTCACCCGTTCACGGGCTAG (SEQ ID NO. 644) TCGAGTAGTAGACGATTGATTAGCCTGAACGTACCTCCGACGGCCAGCTGACGAACGGGTTTGACGGA :::::::::::::Rv74T7D3.seq::::::::::::: TCAGCTGTCTGTAGAAGGGCTGGCGATACTGTGCACTGTCTGATATCGCNNCGTNGTGGGACTATNCAGNCCATNANG (SEQ ID NO. 645) ATGCGGTTCNGNNNNTGCAGAGNATCCTGGNACACATNCGGTTCACGTTAATCANCATCGCGANTTNCTNCGTNTTCG ATTANTTCTGCTAACGNNTCTNNNAGTGCCTGCGGGTCGACTCTAGAG Clone Rv75 :::::::::::::Rv75SP6D2.seq3::::::::::::: NCTCTGCCGGGCNAGAGCGCAGAGTCGGACGGCTTCGTCGATCGTGAAGCGACCNTGCGATGANCAGATATCGNTNAC (SEQ ID NO. 646) ACTGCTCANAAACTTCGGATCATCGNTGATACACAGGCCAACGGGTAGCGGTTGTCCAACCGCTTCGTCAACGANATG GGATCGTGACGANCCTACGCTCGCAGGATATGTCGCNCACCNGNTCTAGANAN :::::::::::::Rv75T7D3.seq::::::::::::: CACTTCATGCTCGTGCGTTGGCNTCGATTTGCNCGAGNGGTTAGCTCCTCGAGTGNGTGACGTATCACTCCGGCNGAC (SEQ ID NO. 647) TANCCGTATCNGCGTCCCGCACCGGTCAACTGGTCTAGCCACACCGGGGAGAATNCNCGACCGGNGCTATCGACCNAT CACGGCTTGTCGNNAAGATAGNCAGCC Clone Rv76 :::::::::::::Rv76SP6.seq::::::::::::: ATACTCAAGCTTGCCAACCGCCACCCTGCATCCGGGGGGCGAGCACTGCTCCGCCGACCAGTACGAACCAACCTGCGG (SEQ ID NO. 648) TGCCCAGGCCATTGACAATGTGCTGGTCGGCGCCCGCGAGTTCTAGCACAGCAACGCCGCGGCCACCACAGGGGCG :::::::::::::Rv76T7.seq::::::::::::: CGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGC (SEQ ID NO. 649) TGTCCAAGATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGT CCACACCGGAAGACCCGGCGTCGGCGCGGTTCGGCAAAACTTTGTGGGATTTCCCGCCCCCCC Clone Rv77 :::::::::::::Rv77SP6.seq::::::::::::: AATACTCAAGCTTCGCGGAGGTGGTGGGGCAGGAGCACGTCACCGCGCCGCTGTCGGTGGCGCTGGATGCCGGCCGGA (SEQ ID NO. 650) TCAACCACGCGTACCTGTTCTCTGGGCCGCGTGGCTGCGGAAAGACGTCGTCAGCGCGTATCCTCGCGCGGTCGTTGA ACTGTGCGCAGGGCCCTACCGCCAACCCGTGCGGGGTCTGCGAATCCTGCGTTTCGTTGGCGCCCAACGCCCCCGGCA GCATCGACGTGGTAGAGCTGGATGCCGCCAGCCACGGCGGCGTGGAGCAACCCCGCGAGCTGCGGGACCGCCC :::::::::::::Rv77T7.seq::::::::::::: GATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAA (SEQ ID NO. 651) TAACTCGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGAAATTGGCAGCAAACCG GCGCTGCTGGGCCGGGCATGGTGCAATCCGAACAAGCACGCTCAACCCGTACACCAACCCCGAAGGGCCGCTGGCCCG GCGGAACCTTGTCCTCCA Clone Rv78 :::::::::::::Rv78SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCTGGGCGTCGTGGTGCCC (SEQ ID NO. 652) GGCCTGCCGGTGCAGGAACTGGATTTTACTGCCATCTCTCGCGACCCTGAGGTGGTCCAGGCTTACAACACCGACCCA CTCGTGCACCACGGACGGGTTCCGGCCGGGATTGGCCGCGCGCTGCTGCANGTGGGCGAGACCATGCCGCGGCGANCA CCGGCATTGACCGCGCCGCTGCTAGTGCTGCACGGCACCGATGACCGGCTGATCCCCATCGAAGGCAGCCGTCGCCTG GTCNAATGTNTNGGATCNGCCGACGTGCANCTGAANGANTATCCCCGGCTGTNCCACNAGGTGTTCAACGAACCGGAN CGCAACCAAGTG :::::::::::::Rv78T7.seq::::::::::::: CAAGGCATACGCCAAGACCCAAGGGATCGCAGTCACCTCCGTCAACGGCCTGGTCGCCGGCCACGGGTCCGTGCAGGA (SEQ ID NO. 653) GACGTGGCTGGCCATGCAAAGCGCCGCCGCCTTATCAGGAACGCCCCGGCTTGTCGGCTTTTCCTGCATCGACACATT TCCGGAGGTGTTGTGGTTGGCGCANCGCGCGAGACAGGCCTGGGATGGCGTGCGCATCGTCATCGGGAATGCGATGGC AACACTGAACTACGAGCGCATCCTGCGCCAGCATGACTGTTTCGACTACGTCGTCGTTGGCGACGGGGANGTAGCGTT CACCAAGCTGGCCTTGGCCCTGGCGAATGACCTGCGGTTGACGACTCCCGGGACTAACCCGCCGTANTGAGCAAGGAC AGATTCTGCGCACACCCTCCTCGCTGGTCGACCTTGACA Clone Rv79 :::::::::::::Rv79SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGCCGGTGATCTGGGTGGC (SEQ ID NO. 654) CAACTCGGCGGGCACCATCTCCATCACGACNGCAAACGCTCCGGCTTCGGCGACAGCGATCGCGTCTGCGATNGTTTG TTCGGCGGCGTCTCCGCGGCCCTGCACCCGGAAGCCGCCCAAGGTGTTGACNCTTTGCGGGGTGAAGCCGATGTGTGC CATCACCGGGATNCCCGCCGCGGTCAGACANGCGATTTGCTCGGCCACCCGCTCACCGCCCTCGANCTTGACNGCATG TGCGCCGCCGTCCTTGAAGAAACCGGTGGCGGNGGCAACCC :::::::::::::Rv79T7.seq::::::::::::: CGTTGAGATCCAGCTGCGCACTGTGCAGCGCCTCGGTGGTCTGCTCGGCCTGCCGGGATAACTCGTTGAGCTTCGCCA (SEQ ID NO. 655) GCGCGTCGTCGGCCGGATCAGCCAGCACATTCGCGGCCAGGACGCCGGAGGAGACGGTGAAGCTCGCAAAGAAACCTA TGGCGGACCGCATGATTACACGCGCGATCAACCACCTCTGGTCGAGCCTCAAAATTTGCTTCCTTAAACGGGCCATCG ACGGATGACGTCGAGCTGGTTTAGGTCCAAACAGGTTACGAAACGATCTCGGAATTGTCCAAAAGGGGAAGTTTAAGA AAATGGATAGATTTCTACCATTTCGCTGTGGACGATCGTACTTCTGCTATAGGGCTCCAGGGGCATCGACACGCAACG ACCTTACGCGACACCGGATCCGCGCTGGCGGCGGAACGGCACCANGCGCAACCGAAGGGCCAATCCGACATCGG Clone Rv7 :::::::::::::Rv7SP6.seq::::::::::::: ATACTCAAGCTTATCTAGGCGCCAGCTTGATTGGTCTGGTTGCATTGGCCAGCTGCGCGAGCCTGGCTCACTTCAACT (SEQ ID NO. 656) ACAACAACCGCAAACAATTGCCGCCTTCGGATCCGAGTTCGGTTGGGTACGCGGCAATGGANCACCATTTCTCGGTGA ATCAGACTATTCCTGAGTACTTGATCATCCACTCTGCACACGACCTGCGAACCCCGCGCGGCCTTGCCGACCTGGAGC AGCTGGCGCAACGTGTGAGCCANATCCCAGGCGTTGCCATGGTTCGCGGTGTGACCCGGCCAAACGGGGAAACCCTTG AACAGGCCCGGGCGACATACCAAGCCGGCCAAGTTGGCAACCGGCTGGGCGGCGCGTCGCGAATGATCGATGAGCGCA CCGGCGACCTGAATCGGCTGGCATCGGGTGCCAACCTGTTGGCCGACAATCTCGGTGACTTCGCGGTCAAGTCAGCCG GGCCGTTGCGGGTGTCCGCAGCCTTGTCCAGCCCCTCGCTTACTCCA :::::::::::::Rv7T7.seq::::::::::::: CAGGCATGCAAGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACG (SEQ ID NO. 657) GATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTG CCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTC GCGGTTGCGCCGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCCG CGGCGATTCAGCCATCGGGACATCATGCTCGCTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGAACG CCCACGCATGGTG Clone Rv80 :::::::::::::Rv80SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGTAGAAAAAGATCGGTGA (SEQ ID NO. 658) GCGCATCGATTCGCTCCGCCGGGTTTGCCGCTGCGGCGGCGGAGCTGCCGTGACCGTCTATTTGGGTGATCAGATACT GGGCTAGTTCGGTCGGGGTGGGGTGATCGAAGATCGCGGTGGCCGGCAGCGTTACTGCGGTGACAGCTGTTAAGCGGT TACGTATCTCCACGGCACTCAAGGAATTAAATCCCGAATCGGCAAACGCCTGGCCAGCGTCNAGTCCGGCAGCGCCGT CNCGCCCCAGCACCGCTGCGGCATGCTCACATACCACCTCGATCGCTGCGGCGANTTGCTCGTCNGCCGACCGACCGG CCANCCGGGCGGCAAACCCNGAAGACCCAAGAATTCATCACCACCATCGCTAGC :::::::::::::Rv80T7.seq::::::::::::: CCTTCTTGACACCCACCTCGCCATCGACCTTGAGCACTCCGTCGTAGTTGGTGAACATGTGACCGGCGATCGGGCGGG (SEQ ID NO. 659) TGAACGCGTACTGGGTGTCGGTGTCGACGTTCATCTTCACCACGCCGTAGCGCAGCGCCTCCTCGATCTCCGACTTAA GCGAACCCGAGCCGCCGTGGAACACGAAATCNAACGGCTTGGCGTCNGCCGGCAGTCCGAGCTTGGCCGCCGCCACCT GTTGCCCTTGCGCAAGGATGTCNGGGCGAANCTTGACGTTGCCGGGCTTGTANACGCCATGCACGTTGCCGAACGTCN CGGCCAGCANGTATTTGCCGTGCTCACCGGCGCCCANCGCCTCGATGGTTTTCTCGAAGTCCTCCGGGCTGGTGTACA GCTTCTCGTTGATCTCGTTCGCCACGCCGTCCTCTTCGCCGCCGACG Clone Rv81 :::::::::::::Rv81SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTGGAAAGGAGATCCCCGGG (SEQ ID NO. 660) AACCTGGTGGCAACCCCGCCATTGGGGTTGTTGGGATTGCCGATCAGCGTGAANGAAGCTCTGTCTGGAGACAGCGGG TCGGCCGAAGCCGCAAGATTGGCCATCACTAGTGACGANATCGTGGCGCTCTGCGAGTANCCNAAGACAGTGACGTTG TTNCCGGCGGCAATTTGCTGCCGAATCGCACTTTCGAGAATGACNGCACCCTGCGCCACCGANGAATCNAAAGTGAGG TTCTTGATCACGACCACCGGGTNGAGCCCTTGGGGCGTGAAGANCGCCTGCGCNATAACACCCGGGACGCTGCCACTC ATGTNCAGCGCGTTCGCGANCTCNACATATCT :::::::::::::Rv81T7.seq::::::::::::: TCCTGGTGATCGANGGCCGCGGTTCCGGCCGAAAATCCGGTTCGGGTTCGGGTCGCGGTTCCAACTTGANCGCGGTCC (SEQ ID NO. 661) GCAGCTGATTCACCGTGGCAACGCCGGCCAACTGCGCATAATGCGCATCCGAACCCTCACCCGCCCGCCCCGCGATCA CCCCAACCTGATCCAACGACAACCGCCCCTCCCGCATACCCCGGGCGCAGCGCGGAAACTCCGGCAACCGCCGCGCCA CCGTGGCGATCGTGTGGGCGTTGCCTGACGAACANCCCATCTTCCAGGCCACCAACCCCGCCACCGACCGCGCCCCCG TCACACCCCACAACCCGTCGCGATCCAGCTCAGCCACGATCTCCACAATGCGCCCATCAATCGCATTGCGCTGAACGG GCAACTCCGCCAACTCCTCCAA Clone Rv82 :::::::::::::Rv82SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGATCTGGTACCCATCCGTGATA (SEQ ID NO. 662) CATTGAGGCTGTTCCCTGGGGGTCGTTACCTTCCACGAGCAAAACACGTAGCCCCTTCAGAGCCAGATCCTGAGCAAG ATGAACAGAAACTGAGGTTTTGTAAACGCCACCTTTATGGGCAGCAACCCCGATCACCGGTGGAAATACGTCTTCAGC ACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTCAATTGTATAACCAACACGTTGCTCAACCCG TCCTCGAATTTCCATATCCGGGTGCGGTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAAACCCCAAC TAAATCCGCTGCTTCACCTATTCTCCAGCGCCGGGTTATTTTCCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAA Clone Rv83 :::::::::::::Rv83SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTANCGCCACCTCCCGGGCG (SEQ ID NO. 663) GAACTCCACGGCGTGGATNAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGANGTCCGCGTT AGCGCCGGATTCCACCACATCCCCTTGCGAAANTCCGTTGGGTNCNATGATGTNNCGCTTCTCCCCNTCNANATAATG GANCAACGCNATCCGTGCGGTACGGTTCGGGTCNTACTCCATGTNCGCGACCTTGGCGTTGANACCATCTTTGTCATT GCGGCGAAAGTCNATCATCCGGTNAGCNCGCNTATGANCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCNTT GCGTCCACCGCGAACGTGCAACGGGGGCNCCAACGANTTCTCCNGGGTTGAACCGGTNATCT :::::::::::::Rv83T7.seq::::::::::::: TGTGTGTGGTGGTAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGCCAGCTCCCAATTGACGTGAGCCCGCTCACTT (SEQ ID NO. 664) GCTGGGTAAGCGTCG Clone Rv84 :::::::::::::Rv84SP6.seq::::::::::::: AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATANAATACTCAAGCTTGCGGGTNATNGCCTTGGT (SEQ ID NO. 665) CAACGGCACCGTGATCGGATCNGGGTCTACCGCACACATNGACTGGAGCTTCGGCGAANTCATCGCCTATGCCTCGCG GGGGGTGACGCTGANCCCNGGTGACNTGTTCNGCTCNGGCACGGTGCCCACCTGCACGCTCNTCNAACACCTCANGCC ACCGGAATCATTCCCNGGCTGGCTGCACGANAGCGANNTTGTCNCCCTCCAAGTCTAAAGGCTGGGCGANANAAGCAN AACGTCCCGACNAACGGCACTCCTTTTCCNTTTGCTCTTC :::::::::::::Rv84T7.seq::::::::::::: GAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCCGT (SEQ ID NO. 666) ACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAA TATGGCGAAAAACGGTCGCCGTTGCACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCACCAACAANGC AATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGTCCGTCATCTGTTTGACNGTGTCCCGTTGGGTATCCGACGT TTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTNTTGCAATGGCCTCATCTTTGATGATCAAATCGATGTN GCTCAGTCTTCCGGGCATATGGAACAACTCTTGGGCCGTGGAAATATCAGCAATGATA Clone Rv85 :::::::::::::Rv85SP6.seq::::::::::::: CTTTCGCCCAGGCCGGCGCGGATGTCCTCATCGCTTCACGAACATCATCCGAGCTTGACGCTGTCGCCGAACAGATCC (SEQ ID NO. 667) GCGCTGCCGGCCGCCGCGCCCACACCGTTGCCGCCGATCTGGCCCATCCCGAGGTGACCGCGCAGCTGGCTGGTCAGG CCGTCGGAGCTTTCGGGAAGCTCGACATCGTCGTCAACAACGTTGGCGGCACCATGCCCAACACGCTGCTAAGCACCT CGACCAABGACCTCGCGGACGCCTTCGCCTTCAACGTGGGCACCGCCCACGCGCTGACCGTCGCGGCGGTGCCGTTGA CGACCAANGACCTCGCGGACGCCTTCGCCTTCAACGTGGGCACCGCCCACGCGCTGACCGTCGCGGCGGTGCCGTTGA TGCTGGAACACTCCGGCGGCGGCAGCGTGATCAACATCAGCTCCACCATGGGCCGGCTGGCGGCGCGGGGTTTC :::::::::::::Rv85T7.seq::::::::::::: TGTGGGCTCCGATCCGGCGCGCATGGCATCGACGGCGACGCCGATCGATGACGGCCAGGCTTACGAGCTTGAGGGTGT (SEQ ID NO. 668) GAAGTTGTGGACCACCAACGGTGTGGTAGCGGACCTGCTAGTGGTTATGGCGCGGGTACCGCGCAGTGAAGGGCNCCG AGGGGGAATCANCGCCTTTGTCGTCGAGGCTGATTCGCCCGGGATCACCGTGGAGCGGCGCAACAAGTTCATGGGACT GCGTGGCATCGAAAACGGCGTGACCCGGCTTCNTCGCGTCAGGGTGCCCAAAGACAACTTGATCGCANGGAAGCGACG GTCTGAAGATCGCGCTGACCACACTGACGCCGGACGGCTGTCCCTACCGGCGATCCAACCGAGT Clone Rv86 :::::::::::::Rv86SP6.seq::::::::::::: GAGCTGGCCGAGCTGGACCGGTTCACCGCGGAACTACCGTTCTCGCTCGACGACTTTCAGCAGCGGGCTTGCAGCGCG (SEQ ID NO. 669) CTGGAACGCGGCCACGGTGTGCTGGTGTGCGCGCCGACCGGCGCTGGCAAGACAGTGGTCGGCGAGTTCGCCGTGCAC CTGGCGCTGGCGGCCGGCAGTAAATGTTTCTACACCACGCCGCTGAAAGCCCTGAGCAACCAAAAGCACACCGATCTC ACAGCACGCTACGGCCGTGACCAGATCTGGCTGCTGACCGGTGACCTGTCNGTCAACGGCAACCGCCGGTGGTGGTGA TGACCACCGAAATGCTGCGCAACATCCTCTAC :::::::::::::Rv86T7.seq::::::::::::: GATCTCTGGATCGGCGGGGCTCTCCGGGCCGGCCTCGGCGACCTCAGCGGGCCGCGCCTTCCGGCCGAACCATTCCCT (SEQ ID NO. 670) AGCCATAGATGACCGCACCTCGATGCACGGTTTGGCGGCAACGCGGCAAGGCGTCNGTCGGGCCCAGCCGCGGCAATG CGGGTACCCGGGAGCGCGGGTCNGTANACCANCGCTGGACTGCGTCGCGCGGTGCGTCNACNTCAAAGTCCCCGGCCT CCCATATCGCGTATGACGCGGGCGCGCCCGGCACCANGGGTGCCGATCCGGCCGTCTCGAACACCACCGGCCCGCCAG CCGCCGCGGGTCCGGCAGCNAACCCGCCCGCGCCGATACCCGCTGCCCGCGTGCGTGATTGACCGCCGCGCGCACGCT GGCCANGGATCAAAGCCCGTG Clone Rv87 :::::::::::::Rv87SP6.seq::::::::::::: GGACGCGTAGCCCGCCAGGCCGGTCAGGGTGCCCTTCCAGTCCACGCCGCTGTGGTCGGCGAACCGCTTATCTTCAAT (SEQ ID NO. 671) CGAGACGATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCACCTCGAACCGGCGCTGCGAGTNCAGCCACGC GATCGTGTTGCCCTTCGCGTCGACCATCGTCGATACCGCAGGCACTTGCCCCTCGAGCAGCTGGGCCGAGCCGTTGGC AACGACCTCAGANGCACGATTGGACATCAGCCCTAGCCCGCCTGCGAACGGGAACGTCAGCGCAGTGGCGACGACACT GGCCAACAGACAGCACCCAGCCAGCTTCAGAACGGTGATCGCGGCCGGGAAGCGCTCGGGCATGCGTNCTACAGTAGC GACCTCCTGTCACTCCACGTGCCGCTCGGTCCAATAGAATCTTTCCGCGGGCGGGTGAATCTCTGCNGGATCGGGGCN GGCGC :::::::::::::Rv87T7.seq::::::::::::: GCTCGTTGCCGGCGGCGATCTCGTCGAGCTCGTCTTCCATCGCCGCGGTGAAGTCGTAGTCGACGAGCCGACCGAAAT (SEQ ID NO. 672) GCTGCTCGAGCAGACCGGTTACCGCGAACGCCACCCATGACGGCACCAGTGCACTGCCCTTCTTGTGCACGTNGCCGC GATCCTGGATGGTCTTGATGATCGACGANTAGGTCGACGGGCGGCCGATGCCCAGCTCCTCGAGCGCTTTGACCAGCG ACGCCTCNGTGTNNCGGGCCGGCGGGTTGGTGGCATGGCCGTCTGGGGTCAACTCGACNATGTCCAACCGTTGACCCG GGGTCAGATGGGGCAGTCGCCGCTCGGCATCGTCAGCCTCGCCGC Clone Rv88 :::::::::::::Rv88SP6.seq::::::::::::: GTCTTTCGATGGCTGCTTCTTCGGCGCTGACGCTGGCGATCTATCACCCCCAGCAGTTCGTCTACGCGGGAGCGATGT (SEQ ID NO. 673) CGGGCCTGTTGGACCCCTCCCAGGCGATGGGTCCCACCCTGATCGGCCTGGCGATGGGTGACGCTGGCGGCTACAAGG CCTCCGACATGTGGGGCCCGAAGGAGGACCCGGCGTGGCAGCGCAACGACCCGCTGTTGAACGTCNGGAANCTGATCG CCAACNACACCCNCGTCTGGGTGTACTGCGGCAACNGCAAGCCGTCGGATCTGGGTGGCAACAACCTGCCGGCCAAGT TCCTCGAGGGCTTCGTGCGGACCATCAACATCAAGTTCCAAGACGCCTACAACGCCNGTGGCGGCCACAACCGCGTGT TCGACTTCCCGG :::::::::::::Rv88T7.seq::::::::::::: GCCAGGTCGAGGTCCCATGCGCGTGGGCCATTGATGCTGATCGCCAGGACGTCAAANATTTGGTCCGGCGTCAGCTGG (SEQ ID NO. 674) GCGAAAAACGTGGGCCCCAGGACTTGCCCGGAGCTGCCCGGGTTCCCGTCGCGCAGCTCGGCGGCCCCGGTCAGAAAN AAATTGCGCCAGGTCGCACACTCCGCGCCGTANGCCAGCTGCTCCAGGGTGTCGGCATAGAGCCCGCGGGCCGCAGCG TGCTCGCTGTCGGCGAACACCGCATGGTCGAGAAGCGTTGCCGCCCAACGGAAATCACCTGCGTCNAANGCTTCGCGG GCCAACTCCAGCACTCGGTCGATG Clone Rv89 :::::::::::::Rv89SP6.seq::::::::::::: NAAACGTTCCGGCTTNGGTGCCGGGCGCTTATTTGCGTCTCTGGGATCACNCTCAGTCGCCGGCGGCTGCCGTTGGGC (SEQ ID NO. 675) TATNANTTGCACCGANCCGGAAAATCCGCACNANAACTGCNAGTAGCGGCCTGCAGAANTGCATCCTCGGCGAANCNG ACTACCGGTGGACANCNACAAGCGCCGCCGAACAACGCACTGGCCCGAGGGATNGGCGTCTATCGGCCCGGCCCGTCG AACTNGGAACAGACNGTGCGGTTCTACCGTGATCTGGTGGGAATGCTCNACCANACCTTCCCNANNGCTACGGAACNA CGGCGCGATATTCNGCCNTCCCANCTCGAGCCTGACNCTNGATATCGTCGANNCTCACCATCNCGATCNGCTGTGCCG GTNTTGCTCGGACTN :::::::::::::Rv89T7.seq::::::::::::: CGAACGACGAACNCCNCAAGCCATGGTGGTTGGCGCCGTCAAAAGGTCCGCGGTCGCCACTACTGGAAAATCGCCTTG (SEQ ID NO. 676) AGCGTCNCTCGACCNCCGCCTCGAGTTGGGTCNTAACGAAATACCTGATGCCGATCANGTCNACGTCTCCGTCGCNNC AACGTGCAGCGGCGACCCACTCTACNANCTCTCGGTNCCGCCNCGGCCAGNGCACCACCAGTGACNAATCCNTGCGCC NTCGGGCCNAGCANTCCCGGTGCNACCGNGGTGGGTCCGGCGATGGTNGGGTGTNCTCNNTACNGGAACGCCAGCGCN ATCANCATCGGCANACTCNCGTCGATGTGCCGCGGCGCAACCATCCCCCACAATGATCNGGTGCGTCTGATCAGGCN Clone Rv8 :::::::::::::Rv8SP6D.seq::::::::::::: TTAGGCGTGACGGCGACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACGATCGAATACGACGGCGTC (SEQ ID NO. 677) GCCGACTTTCCGCGGTACCCGCTGAACTTTGTGTCGACCCTCAACGCCATTGCCGGC :::::::::::::Rv8T7D4.seq::::::::::::: CGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAGATAAAGCACGAACTGGCGGGCAAAACGTCGATCTCGGAGCCGG (SEQ ID NO. 678) AAGGGCAATCAGCCGACCGTCGACGAACGACACCGGCGAGACCACTTAGGCAGTGACGGCCT Clone Rv90 :::::::::::::Rv90SP6.seq::::::::::::: CTTTTCNCGATGTCTCATGATNCCNANGGAGAACNNTGCNANCNCNGCCGCTGACNTNGCNCACCGCTNTGGCNGNGG (SEQ ID NO. 679) TGACATTGGTGGTGGTTGCGGGCTGCNACGCCCGACTCGANGCCGANCCATNTNTTGCGGCCGACCGCNTNTCGTCTC NACCGCANNCCCNATCTCNGCCGCNCCCGGTGGANCTACNGCTNCTTCGCCATCTCTCGCCNATGGCTCCNGCGNNTC GCNCAACGTNTGGTTTGGTNANCTGCCTACCTGGTCNT :::::::::::::Rv90T7.seq::::::::::::: GCTGCGCCAGTCGTTCGGTGCGGTCATGCCGTTGGACCNACCATCGGAGTTAGTTGCCGAACCGCGGACCACCGCAAG (SEQ ID NO. 680) CACCCGGTCCTGCTCGCGCACCGCGTCGGCCAACCGCTTGAGCACCACCACGCCGCAGCCCTCGCCGCGCACGAATCC ATCCGCGTTGGCGTCNAANCTGTNGCATCGGTCGGTCGGTGACAGCGCCGACCACTTGGACAGCGCGATGGCGGTGAA CGGTNANTAGGTGACCTGCCNCCNCGCCCGCCAATGCCCACCTCCGCTTCACNCATGCGAATGGTCTGACACGCCNAG TGAATTGCCACCAGCGACAACAAAAATCGGTATCTNCNGCGACGGCGGACACGCNATCCCNACTGATACTCGATCCGC CCCACCGCTTGNANCTCCGGGTTCCNGTGCTCATGTACCNTCATGTCGGTCTGCGCNCGATATTGACCATCGTGTTTC CCACGANNANAGANCCTCATCACGCCGGTTCGAGTGCCG Clone Rv91 :::::::::::::Rv91SP6.seq::::::::::::: CTGTGTGCGGNCGGCGCGATATCGGCCTTTTTACTAACCGAACCCGATGTGGGCTCCGATCCGGCGCGCATGGCATCT (SEQ ID NO. 681) ACNGCGACGCCGATCGATGACGGCCAGGCTTACGAGCTTGAGGGTGTGAANTTGTGGACCNCCAACGGTGTGGTAGCG GACCTGCTANTGGTTATGGCGCGGGTACCGCGCAGTGAANGGCACCGAGGGGGAATCANCGCCTTTGTCGTCTANGCT GATTCTCCCGGGATCACCNTGGAGCGCNCCNCNANTTCATGGGACTGCGTGGCATCCAANACGGCGTGACCGGCTTCA TCCNTCNGGGTGCCCAAAGACAACTTGATCNGCNNGGAAGCGACGTCTGAANATCGCGCTGATCNCACTCAACGCCGG ACGCTGTCCTACCGGCGATCGCACCGGANTTGCCAANCCGCGCTNANNATNCGCGNGAATGNCCGTCCACNANTGCAT GG :::::::::::::Rv91T7.seq::::::::::::: TGGGGTGCCGGGCGCCGAGTTGCGTCCCTGGGATCACGCAGAGTCGCCGGCGGCTGCCGTTGGGCTATGAATTGCACC (SEQ ID NO. 682) GAGCCGGAAAATCCGCANCAAAACTGCGAGTAGCGGCCTGCAGAAGTGCANCCTCGGCGAAACGGAGTACGGTGGACA ACGAAAAGCGCCGCCGAACNACGCACTGGCCCGAGGGATTGGCGTCAATCGGCCCCGCCCGTCGAACTTGGAAGANAC ANTGCGGTTCTACCGTGATCTGGTGGGAATGCTCCAACNNACCTTCNCGGAAAGCTACGGAAGCNACGGCGCGATNTT CGGCCTTCCCAGCTCGACCTGACGCTGGAAATCG Clone Rv92 :::::::::::::Rv92SP6.seq::::::::::::: NGGCNGGGAAGTTAATGCCCTACTGGTTCNATGCTCNCACNTCNCCNGTGACNNCCTGCNCCGACCCGCCGAGGTCCT (SEQ ID NO. 683) GNCCGTNACCACCGANCNGGCGATCCGGGACTCTNGTACGCATCCAACANNGANCAACGTGCACGGGCGGAGTNGTNC CGCCACTTCGNCNATGACGGGGTCGATCCNTTCGACGTCCGTCGCCGCGTCGCTCGAGTGGCGGTCACNCTCCNNGTA CTCGACCNCACNGACGAGAGGACTCGANCCCATCTACGTGTGGACGAAACANATCTTCTGTCCNACGACTACACCACC ACCCAGGCCATCGCCGNCGCCCGCGANGCCCCTTCGACGCCNTACTGGTCCNGNGGNGGCGCTCTCCGGTTGTCTNNC NCNTGNCGTGTTCCTTCACNCACTGCCCNACATCGANCCCGAGCNATNCNANGTCCGTCAATC :::::::::::::Rv92T7.seq::::::::::::: GGACACTGTTCGCGTGCCCCTCGTCAAAGCCGGAGTGGTCGTGCTGCGCCGGACCCGACCCGACCTTCAGCGGGGGTT (SEQ ID NO. 684) CACAGCTCCGTGGGTGCCGTTACTTCCGATCGCCGCAGTGTGCGCGTGCCTGTGGCTGATGCTGAACCTCACCGCGTT GACTTGGATCCGGTTCGGGATCTGGCTGGTGGCCGGAACCGCGATTTATGTCNGCTACGGGCCCCGGCACTCGGCGCA TGGCCTTCGGCAAGCNCNANANAACGCGACCCGGAGGTGTTGAACTAGCTTCGCCGCGTATTTACAAATTGCNTTATA TGTCTACACATAAGACGCAAACTGCTCTATTGTCAANTCCCANCGTGGTGTGGCNCATGAAGATGTTTGG Clone Rv94 :::::::::::::Rv94SP6.seq::::::::::::: TCCTTCTCGGTATCGGTTTGGGCTGTCACCANCAGTTGGTAGTTCTTCACGTNCTGTTGTTCGAGCGTCNAGCCGTCG (SEQ ID NO. 685) CGCGTGTCNANGTCNCCGGACGCGTATCCCGCCAGGCCGGTCANGGTGCCCTTCCANTCCACGCCGCTGTGGTCGGCG AACGCTNATCTTCAATCGAGACCATCGCCAGCTTCATCNTGTTGGCGATCTTGTCNNACGGCACCTCNAACCGGCGCT NCTAGTACNCCACNCNATCNTGTTNCCTTCNCGTCNACATCCTCGATNCCNCNTGCACTTTCCCTCGANCNCCTGGGC CGAGCCGTTGGCANTNACCTCNGAGCCCCATTGGACATCANCCCANCCCGCCTGCGAACGGGAACGTCAGCNCNCTGG CGACAACCTGGCCAACAN :::::::::::::Rv94T7.seq::::::::::::: CACNCCGTGATCGCNAGCCCCNGTAGAAATNGTTGAGCCAGTTGGTGCGGCGCTCGTTGCCGGCGGTNATCTCGTCGA (SEQ ID NO. 686) GCTCNTCTTCCATCGCCGCGGTGAAGTCGTACTCGACNAGCCGACCNAAATGCTGCTCNAGCAGACCGGTTACCNNNA ACNCCNCCTCNTGACNGCACCAGTGCNCTGCCCTTCTTGTGCACGTACCCGCNATCCTGGATGGTCTTGATGATCNAC TANTNTGTCGACGGGCGGCCGATGCCCATCTCCTCNAGCGCTTTGACCAGCGACNCCTCGGTGTATCGGGCCGGCGGG TTNGTGGCATGGCCGTCTGGGGTCANCTCNACNATNTTCANCCGTTGACCCGGGGTCACA Clone Rv95 :::::::::::::Rv95SP6.seq::::::::::::: TGGCCTTCTTGNCANGGGCNNACATNNGCTATNGCGAGCGTGTAACCGATCATCNTCCNGGCGACTGTGGCCTGANCG (SEQ ID NO. 687) GCAAGGGTNGCCTNATTCNTCCTCCTGNGGCATGGTTNCCACACGGAATGNCGGTAAGTCTGGTCAACAACCTGGCCC GCTGCGGGTTGGGTTCGGATTCGCTCGGCTANTAAGGTGCTCGCCTGGTGTNACNACTAATCNCNATATACNCTTANC GGGAGTNGNCGTCCCGATCCTNGCCCTGCCGCNGGCGATCNCGTTCGCANCACCGCCACCGGAACTCNCAANGTGCGC TCATCGGGCTCTACGCGCCATCTTCCCCGGATTCTTCGCGGCNGNGTNCCGNGGGACCCCGGACTGTGACNGGCCCAA CGGCTCATCATCG :::::::::::::Rv95T7.seq::::::::::::: CCGGATAGCGGTGTCTGAACTTCGCCCGTTCCCTCCANCGCATTGAGCTTCAGCCCGACCGGCAGGTNNGGAGTCGGC (SEQ ID NO. 688) ATGCGGTCCTTCGCCCCGACCCCGCTGGCTAAATANCCACCCCCGAGCGCGGTCACGGTCTTTGCACCGGGACGACGC ATACCGGCAGCGCGAACATCNCCGCGGGCTGCAGCNTGAACGTCCAATACCANTCNAACAGTGTCCGCGCGTNAAAAC CCGANCCGGCGGTCGCTTCNGTAATCAACGGCTCCTGCGCAACCAGCTGCAAGTCGCCGGTGCCACCGGCGTTGACGA TCTTGATGTCTGCGANCTCGCGCACCAGCTCGACGGCCCGGGCA Clone Rv96 :::::::::::::Rv96SP6.seq::::::::::::: CCTCCCGACCACATACAGGCAAAGTAATGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAATCCACCA (SEQ ID NO. 689) TCGGGGCAGCTGGTGTCGATAACGAAGTATCTTCAACCGGTTGAGTATTGAGCGTATGTTTTGGAATAACAGCCGCAC GCTTCATTATCTAATCTCCCAGCGTGGTTTAATCAGACGATCGAAAATTTCATTGCAGACAGGTTCCCAAATAGAAAG AGCATTTCTCCAGGCACCAGTTGAAGAGCGTTGATCAATGGCCTGTTCAAAAACAGTTCTCATCCGGATCTGACCTTT ACCAACTTCATCCGTTTCACGTACAACATTTTTTAGAACCATGCTTCCCCAGGCATCCCGAATTTGCTCCTCCATCCA CGGGGACTGAGAGCCATTACTATTGCTGTATTTGGTAAGCAAAATACGT Clone Rv9 :::::::::::::Rv9SP6.seq::::::::::::: CTTCACNTCCGTACGGCTCGGGTACGCTTCGGTCNCATTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATC (SEQ ID NO. 690) TTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTANAAGGTCGGCGANCGCTCNGCATTGGTCAT CGGGATATGCCGCTCGGGACGGTCANAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACG CGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTANACGGTTCCTTTCCTAAGCAACACCGA ANTTTCAGGACCCGAATGCTCCGGGAAACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGANCATTGAGCACGC CGGCCAGCACCGCACGAACCAGGCAATCAGCCGCCGCCGCACCCGACCGCGG :::::::::::::Rv9T7.seq::::::::::::: CAGGCATGCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCC (SEQ ID NO. 691) CGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACCTCGAGCCATACCGG GCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGN3GTCGAGGTCGATACCGATTGGCGCATCCGC AGCCGCACCCTGGACGACAGAACCGTGCCCTACGAATTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGC GCGATTGGCCGGCGCGGCGCTGGTCGCCAAGGAAGACCCGTTCCGGTGCTGAT

TABLE 4 End-sequences of the polynucleotide inserts cloned in the named recombinant BAC vectors contained in the I-XXXX M. bovis strain Pasteur genomic DNA library. RvXXXSP6 corresponds to the SP6 end-sequence of the clone RvXXX. RvXXXT7 corresponds to the T7 end-sequence of the clone RvXXX. RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081 repetitive sequence (Insertion element). The character << - >> denotes an uncertain base residue. Clone X0001 ::::::::::::::::X0001SP6.seq:::::::::::::: AAG- (SEQ ID NO. 692) TCGGGTTTCCACACGCGCGGTTTGACCCTAGTCATATGTAATCATGTGTACCATGTGCGGGCGCTTTTCGACGGCCG CGAACCACCGGA-ATTTCCTGTGATTTCACTGCATGCGTACCATCTGGCACAATTGAGCA-TTGTCT- TCGCGGTGGTCGG-CGGGTTGCGTGCCGCCTGCTGCGA-ATGCACCA- TAAGCCCGAACCCACCGGCTTGGTGACCACCGCACGCTGCGTGTGGGGGGTAACCACTCCGCGACCCCAAGGATGGT CATTTCCAATGAACCGGCTGGACTTCGTCCA-A ::::::::::::::X0001T7.seq:::::::::::::: GTCGCGGTTCGATCGACCCGATCTTCACCTCGTAACCTCGATGCTTAGCAGGATCCAGCTTGACCGCGTTTCGCTCT (SEQ ID NO. 693) ACCCACTCTTTGAGTGGCGCCGTCGCCTGTGCCCCATCGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCTTGTG AGCCGGAATGTCTGCGTAAAGAAGTTCCATGTCCGGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCCTTCGAGG TCCGCTTCTCGCCGGATCCGATAAACACCGGCCCCAGGCACCGCAGCGTGAGTTCGAACGGCTTCAGGTAGGTGTTC ATGCGGCGGACTCCGGGAGTGCGAGAAATAGCGGTCGCGCGTAGCTGTAGACCGGATGGTTTCCGCCCAGGCTGACG TCGAAGATGCCTCCTTGGAAGGGGCGCGA Clone X0002 ::::::::::::::X0002SP6.seq:::::::::::::: AACTCAAGTTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCA (SEQ ID NO. 694) ACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAA CCAGCTTCCATATCCCGCGAC- AACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGG TGT ::::::::::::::X0002T7.seq:::::::::::::: GTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCA-GCTATCGCACCCGTT (SEQ ID NO. 695) ATCGGCTGCGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGG AAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTC GCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTCGTGGCAGCTCGAA AGGGTCCTGCCGGTGCCGGT Clone X0003 ::::::::::::::X0003SP6.seq:::::::::::::: TTCGAGTCATGCGCCCGCCTCGACCACGAA-ATGCACGTCG- (SEQ ID NO. 696) GGTTCGATCGACCCGATCTTCACCTCGTAACCTCGATGCTTAGCAGGATCCAGCTTGACCGCGTTTGGCTCTACCCA CTCTTTGAGTGGCGCCGTCGCCTGTGCCCCATCGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCTTGTGAGCCG GAATGTCTGCGTAAAGAAGTTCCATGTCCGGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCCTTCGACGTCCGC TTCTC ::::::::::::::X0003T7.seq:::::::::::::: GTCATGTGTACCATTTGCGGGCGCTTTTCGACGGCCGCGAAACACCGGAGATTTCCTGTGATTTCACTGCATGCGTA (SEQ ID NO. 697) CCGTCTGGCACAATTGAGCAGTTGTCTGTCGCGGTGGTCGGCCGGGTTGCGTGCCGCCTGCTGCGAGATGCACCAAT AAGCCCGAACCCACCGGCTTGGTGACCACCGCACGCTGCGTGTGGGGGGTAACCACGCCGCGACCCCAAGGATGGTC ATTTCCAATGAACCGGCTGGACTTC-TCAACAA Clone X0004 ::::::::::::::X0004T7.seq:::::::::::::: AACAGCGCGGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGGCCATTTGTTCGATGCGGTTACCGAAAGAT (SEQ ID NO. 698) CTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCTTGGCGT CCACGGTGGTCGGGG Clone X0006 ::::::::::::::X0006T7.seq:::::::::::::: GCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCCGTCGAACACCATCGCCAGCGCGGCTTCCACATCAACGACCA (SEQ ID NO. 699) TTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGATGAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCGTA- CACAGCGATTCGACCAGCGCGCGGCGCGCGTTGCCGAGGGCGAACGAAGCGGTGCCCAACCGCAATCTGTTGGTCAG CTCCATCATGCGGGTGAGTCCCTTGCCG Clone X0007 ::::::::::::::X0007SP6.seq:::::::::::::: ATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCA- (SEQ ID NO. 700) GGCCGCTCCCGGGCGACCCGACCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTA GCTCCACCATCGCCTGCACACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTA- AGCAGGTAATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGC GGGCATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTC ::::::::::::::X0007T7.seq:::::::::::::: AGCGGTTTCCCA- GCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCATCTTCTGACGCGTAACC (SEQ ID NO. 701) GCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGGGATGTTGATGTCTGTCGAT CTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCTACCACCGTGACCTTCCTGAGGA AACGCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACCGAATTCGTCAATCTGCACG Clone X0008 ::::::::::::::X0008SP6.seq:::::::::::::: CAAGCTTCCACAGGTAGGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGCCATT (SEQ ID NO. 702) TGTTCGATGCGGTTACCGAAAATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGC CGCCGCGGCAACGATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCGGCCGGTCAGCC GGGTGAACTTCGTCGAAAGCTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTANCTCCACCAAGCCCGG GCAACCTCGGGGGTGGCGCC ::::::::::::::X0008T7.seq:::::::::::::: TGGACCTCATGACAACGCGGCGGCGATTACCCCCGCTACCGCCAGCAGCATGACGGCGGTAGCGAACACCGCCGGAT (SEQ ID NO. 703) GCAGCGCAGGTGCGTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGTGCCACCCCCTGC AGCGCGACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGGCGTATATGGCGGCGATGGTG ACGATGGCCAGCGCCACATACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACG CAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAGCCTGCGA Clone X0009 ::::::::::::::X0009SP6.seq:::::::::::::: TTTGGTGCGGCCGGCAATCAACTTC-GCTC CAGCGGTTTCCCAGGCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCATCT (SEQ ID NO. 704) TCTGACGCGTAACCGCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGGGATGT TGATGTCTGTCGATCTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCTACCACCCT GACCTTCCTGAGGAAACGCTGCGTTGGCTCTACGATATGNDGGTGGTCACCCG ::::::::::::::X0009T7.seq:::::::::::::: CGCCCAGGGCCGCTCCCGGGCGACCCGACCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCGTCG (SEQ ID NO. 705) CTGTCTAGCTCCACCATCGCCTGCACACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTAATCCTC GTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGCATCGGGATGG ACGACGACGCGGTGCTGACGGCGCCTGTCGCGACGCTGAGCTCGGACACAGCTAGTAAATGTAGCCTAACCTACTTA ATGGGTCGCAGCCCCCCGGGGTCGTCGCATGTCCAACGTTGCTCGACTGGAAGAAAATGCTCGTCGGGGAGCAAATG GCACC Clone X0010 ::::::::::::::X0010SP6.seq:::::::::::::: AATACTCAATCTTGATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCAGGGCCGCTCCCGGGCGACCCGACCA (SEQ ID NO. 706) TTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTAGCTCCACCATCGCCTGCACACCGG CGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTAATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGC TCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGCATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTCGC GACTCTGAGCTCGG ::::::::::::::X0010T7.seq:::::::::::::: GGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCATCTTCTGACGCGTAACCGCG (SEQ ID NO. 707) TTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGGGATGTTGATGTCTGTCGATCTT GAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCTACCACCGTGACCTTCCTGAGGAAAC GCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACCGAATTCGTCAATCTGCAGCGCCAGGGGG AAGCTGGCGTTGTACACGCCCTGTCGCGGGCAGGAAGCCGCGCAGGTGGGTGCGGCGGCTTGCCTACGCAAAACCGA CTGGTTGTTCCCC Clone X0012 ::::::::::::::X0012SP6.seq:::::::::::::: ATCACGACAACAGCGACGGTGTGTCGGATCAGCGGCCCCCGTTGCCGGGCAATGTTGAGGCGTTTCTGCGTCTGGTT (SEQ ID NO. 708) GAGGCCGGCTGGGAC- CCGAGGTGGCTCGTCGGCCACATGGGCAGCACACCACCGTGGTGATGCATCTAGACGTGCAGGACCGTGCCGCTGGC CTGCA ::::::::::::::X0012T7.seq:::::::::::::: GCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTGCGAGCAAATCGCGGTATGCGTTC (SEQ ID NO. 709) TTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGC CGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTG: Clone X00013 :::::::::::::X0013T7.seq:::::::::::::: TACAAGCGGCACCTCGCCGGTGAACTGACCGTTCGCACGCTGCGCACCGCCGCCGGGCGCGTGCTCGGCGCGCCCGC (SEQ ID NO. 710) GGCCCCCGAGGCCTGAGAGGGGAACCAACCATGCAGGTGAACATGACGGTAAACGGCGAGCCCGTCACCGCCGAGGT CGAACCCCGGATGCTGCTGGTCCATTTTCTCCGTGATCAGCTGCGGCTCACCGGAACTCACTGGGGCTGTGATACCA GCAACTGCGGGACATGCGTGGTGGAGGTCGACGGCGTGCCGGTGAAATCCTGCACGATGCTCGCCGTGATGGCCTCC GGGC Clone X0014 ::::::::::::::X0014T7.seq:::::::::::::: AGCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCG-CC- (SEQ ID NO. 711) TCCGATGCTGGAAGCCTACACTGCCCTTGGTGCGCTGGCC-C-GCGACCGAGCGGCTGCAACTGGGCGC- TTGGTGACC-GCAATACCTACCGCACCCC-ACCCTGCTGG-CAAA- ATCATCACCACGCTCGACTTGGTTAGCGCCGGTCGA-CGATCCTCGGCATTGGAACCGGTTGGTTT- Clone X0015 ::::::::::::::X0015SP6.seq:::::::::::::: ACGCGCGCCGATCATATCTGCTATGGATGTACAATTCAGCTCTTGCTGTTATACCAGTATATGGTGTACTATTTGAT (SEQ ID NO. 712) CTATGCTGACGTGTGAGATGCGGGAATCGGCCCTGGCTCGACTCGGCCGGGCTCTGGCTGATCCGACGCGGTGCCGG ATTCTGGTGGCGTTGCTGGATGGCGTTTGCTATCCCGGCCAGCTAGCTGCGCACCTCGGGTTGACUCGATCGAATGT GTCCAACCATCTGTCGTGTTTGCGGGGCTGCGGGCTGGTA-TCCCAACCTATGAGGGCCGGCAGGTTCGGTAT ::::::::::::::X0015T7.seq:::::::::::::: CCGCGCTGCTGCTGACGTCGGTCGAACGTGCGACACGTCTGCGAATACCGGCCGAACGCTGGGTTTATCCACAGGCT (SEQ ID NO. 713) GGCACCGACGCCCACGACACACCGGCCGTCGCCGACCGCCACCGACTGCATCGGTCGACGGCCATTCGGATCGCCGG TGCCCGGGCGCTGGAACTGGCTGGGCTGGGGCTCGATGACATCGAATACGTCGACCTGTATTCGTGCTTTCCCTCCG CTGTCCAAGTCGCCGCAATCGAACTCGGCCTGGACACCGACGATCCTGCCCGCCCGCTGACCGTCACCGGGGGCCTG ACCTTCGCCGGCGGGCCGTGGAGCAATTACGTCACGCACTCCAT Clone X0016 ::::::::::::::X0016SP6.seq:::::::::::::: CAGGCGTGCAATGACCTGCACTGCGCCGGA-A- (SEQ ID NO. 714) TCCCTAACCCACTAAACCGGGGCCGCTCACAAGCCGTGCAGCTCGGTCAGCGTCAGGTGCGCGACCAGGAA- TAAATGAGCAGACCCGTGCCGTCAACGATGGTGGCGATCATCGGCCCCGAAACGATGGCCGGGTC- ATGCGCAACTTCTTCAGCAGCGGCGGAAGGACGGCA-CCACCAGCGAC-ACCACACCACGAT ::::::::::::::X0016T7.seq:::::::::::::: GCGAA- (SEQ ID NO. 715) CACTTCGTCAACTTCCAGGGCTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCCGCCGCCGCCGGCGCGC GGCAGGTGGTCATCCTGGCGGCGGGGCTGGACTCGCGCGCGTACCGGCTGCCTTGGCCCGACGGGACCACGGTTTTT GAGCTGGACCGCCCGCAGGTCCTTGATTTCAAGCGCGAGGTGCTCGCCAGCCACGGTGCCCAACCGCGCGCCCTGCG CCGCGAGATCGCCGTCGACCTGCGTGACGATTGGCCACAAGCCTTGCGGGACAGTGGTTTCGATGCGGCTGCACCGT CGGCATGGATTGCCGAAGGGCT Clone X0017 ::::::::::::::X0017SP6.seq:::::::::::::: TTGGGC-TTGCCC-CAATA-GGCCCCAATCAAAAGCCGAGCAGGTGGAACCTA-CGCATTCGCCTC-TCGT- (SEQ ID NO. 716) TGTGCACCCGAGCCATCGCACGCGCGGGAATTCCCGGAT-TC- CCGTATTCTCCGGCGGCCGGGCTAACCCATCCCA-GCCGAACGGTTGGCTC- TGCCGTGGGTCCCGTGTTGGCCGATCGGGGCGTCACCGGGGGTGCTCGGGTGCGG-TGACCATGGC-AACTGCCCC- ATGGGCCGACCCTGGTGCAGATAAACCTG ::::::::::::::X0017T7.seq:::::::::::::: TGGTGGAGGTCCCCACCAA-ACCCGGCCGTAACTCTGCTCACGGAAATGCGG- (SEQ ID NO. 717) CAGGCCGCGCGTAGCACGTGGTATCCGCCATAAAGGTGCACCTTAAGCACGGCGTCCCAATTCTCGAACGACATCTT GTGGAAGGTGCCGTCGCGCAAGATCCCGGCGTTGCTCACCACACCGTGCACGGCGCCGAATTCGTCAAGCGCGGTCT TGATGATGTTCGCTGCGCCGTCCTCGGTGGCGACGCTGTCGGTA- TTGGCGACCGCCCGGCCCCCCTTGTCGCGAAATCTCGGCGACGACCTCATCGGCCATCGCCGAACCGGGCGCCCG Clone X0018 ::::::::::::::X0018SP6.seq:::::::::::::: GCCGGCCAAACTGGCCGGCGGGGTTGCTGTC-TCAAGGTGGGTTCCGCCACCAA-ACC- (SEQ ID NO. 718) CACTCAAGGATCGCAAGGAAAGC- TCAAGGATGCGGTCGCGGCCGCCAAGGCCGCGGTCAAGGAGGGCATCGTCCCTGGTGGGGGA- CCTCCCTCATCCACCAGGCCCGCAAGGCGCTGACCGAACTGC-TGCGTC-C-GACCGGTGACAA- GTCCTCGGTGTCCACGTGT-CTCCGAAGCCCTTGCCGCTCCGTTGTTCTGGATC-CC-CCAAC- CTGGCTTGGACGGCTC-GTGGTGGTCAACAAGGTCAGCGAGCTACCCGCCGGGCATGGGCTGAACGTGA Clone X0018 ::::::::::::::X0018T7.seq:::::::::::::: CGAACCT-AATTGTCCTGTAATGCCCAGCTCACCAA- (SEQ ID NO. 719) GCATGGCTGGTGGCCGGGGCGGTGAAGCCGGCGTCTGCGGCACCGTCCAACTC-ATGTGGAT- GCCGGAATGGGGATGTCCGG-ACGGCGAATCCGTA- TTCGCTTGTCCCGTGAGGCCCAGGTGGATGGGGGGAAGGATC-TGGTGTCCGGGATGAT- ATGGGGCCGATGCCGCCGGTTGAAGTCCACTGGATCGGGAATTCGGGAATCGTGAT-CCGACGTTCAGGCCGAAC Clone X0019 ::::::::::::::X0019SP6.seq:::::::::::::: CTAACGGAATGAAAGCCCTGGTGGCCGT- (SEQ ID NO. 720) TCGGCGGTGGCCGTCGTCGCACTGCTCGGTGTATCTTCCGCCCAAGCTGATCCCGAGGCGGATCCCCGCGCAGCTGA GGCCAACTATGGTGGCCCCCCAAGTTCCCCACGTCTTGTCGATCACACCGAATGGGCGCA- TGGGGAATTCTGCCCAGCCTCCGGGTCTACCCGTCCCAAGTTGGGCGTACA- CCTCCCGCCGCCTCGGGATGGCCGCTGCCGACCCGGCCTGGGCC- AGGTTCTCGCGCTGTCACCGGAAGCCGACACTGCCGGC ::::::::::::::X0019T7.seq:::::::::::::: CCGCGGGACAC-CCTC- (SEQ ID NO. 721) ATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGG CGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGC GGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCA- ACGACATCGTGGCGAAGATTCGCCGGGTACGCCGATGATGTGGTGTGTTTGGCGACGCCGGCGTTGT Clone X0020 ::::::::::::::X0020T7.seq:::::::::::::: CTCTGGGACCGGCCACGGTGCC- (SEQ ID NO. 722) CCGGCGTTCCCGGACGTGCTGCGCCAGGTGTCCGGCGGCCGCGTGCATGGTGTTCCCGGATCGCCCGCTGGCCAGAG CCCACCGGTGAATCTGGCGCCTGGCCGACCACCGTGCGCCGTAGGCTTGCGATCGTGCAGCGCTGGCGTGGCCAGGA CGAGATCCCGACGGATTGGGGCAGATGCGTGCTCACCATCGGGGTATTTGACGGCGTGCACCGCGGGCACGCCGAAC TGATCGCGCACGCGGTCAAAGGCGGC Clone X0021 ::::::::::::::X0021SP6.seq:::::::::::::: AATACTCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAA-AGCATCGGGACATACGGA- (SEQ ID NO. 723) TCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCAC- TTCAGCAAATGGCCA-CGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATC- ACATC-TCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGAC- ATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGCCGCGCTGGCGGGCTGATTAAC ::::::::::::::X0021T7.seq:::::::::::::: TGCCGCGGATTTGGCTGGCTGCCCAATATTCAGAATCGGGCCTTTCTTTTTGCGCGACAATAAGGTCACAGTAAACC (SEQ ID NO. 724) CTCGTTTTGTGAGATGCGGGGCGGGCCGGGCGAA- TCGACCTCGAGTGAATGGATCTCGAGTGAATGGACAGGGCATCGCCTACGAGTCGCATCCCCATCCAACAGACCGGT GCTCTTGCATCGGACCCTGAAGGTCCCGCACGGAGGGTGTGGTTGCCGGCGCGGGCTCACGGTGCGGTAGCGACGTA GTGTTTGAACGAATTTCTTGATGCTCCAACCTGTTTGGTGTTCAATCCAGTTCT Clone X0175 .....X0175SP6..... AA-CTTGCGCGCTCGGCCGGGTC-AGCATCCAGCTGCTCGGCAAGAGGCCAGCTAC-C- (SEQ ID NO. 725) TCGCTGCGTATGCCCAGCGGTGAGATCCGCCGGGTC- ACGTCCGCTGCCGCGCGACCGTCGGCGAAGTGGGCAATGCCGAGCAGGCAAACATCAACTGGGGCAAGGCCGGTCGG ATGCGGTGGAAGGGCAAGCGCCCGTCGGTCCGGGGCGTGGTGAT-AACCCGGTC- ACCACCCGCACGGCGGTGGTGAGGGTAAAACCTCCGGCGGCCGTCACCCGGTTAGCCCGTGGGGCAA .....X0175T7..... A-TCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAACG (SEQ ID NO. 726) CTTGCAGTGCCGCCGAATAGGCGGCTACGTCGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCTGGGCC GCGCCGACGTCGACCGTGGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCGATCGTCGACCCGCACGGCAGATCGGC GCGATGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTGCGAACGCCGCCGCGGTGCGTCCG

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1. A purified polypeptide, encoded by a polynucleotide comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from: (a) nucleotide 1,696,019 through nucleotide 1,697,420 of the Mycobacterium tuberculosis chromosome; (b) nucleotide 1,696,019 through nucleotide 1,699,892 of the Mycobacterium tuberculosis chromosome; (c) nucleotide 1,696,019 through nucleotide 1,701,088 of the Mycobacterium tuberculosis chromosome; (d) nucleotide 1,696,019 through nucleotide 1,702,588 of the Mycobacterium tuberculosis chromosome; (e) nucleotide 1,696,019 through nucleotide 1,704,091 of the Mycobacterium tuberculosis chromosome; (f) nucleotide 1,696,019 through nucleotide 1,705,056 of the Mycobacterium tuberculosis chromosome; (g) nucleotide 1,696,019 through nucleotide 1,705,784 of the Mycobacterium tuberculosis chromosome; (h) nucleotide 1,696,019 through nucleotide 1,706,593 of the Mycobacterium tuberculosis chromosome; (i) nucleotide 1,696,019 through nucleotide 1,707,524 of the Mycobacterium tuberculosis chromosome; or (j) nucleotide 1,696,019 through nucleotide 1,708,648 of the Mycobacterium tuberculosis chromosome.
 2. A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from: (a) nucleotide 1,696,728 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (b) nucleotide 1,698,096 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (c) nucleotide 1,700,210 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (d) nucleotide 1,701,293 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (e) nucleotide 1,703,072 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (f) nucleotide 1,704,091 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (g) nucleotide 1,705,056 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (h) nucleotide 1,705,808 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (i) nucleotide 1,706,631 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; or (j) nucleotide 1,707,530 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome.
 3. A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from: (a) nucleotide 1,696,019 through nucleotide 1,698,096 of the Mycobacterium tuberculosis chromosome; (b) nucleotide 1,696,019 through nucleotide 1,700,210 of the Mycobacterium tuberculosis chromosome; (c) nucleotide 1,696,019 through nucleotide 1,701,293 of the Mycobacterium tuberculosis chromosome; (d) nucleotide 1,696,019 through nucleotide 1,703,072 of the Mycobacterium tuberculosis chromosome; (e) nucleotide 1,696,019 through nucleotide 1,704,091 of the Mycobacterium tuberculosis chromosome; (f) nucleotide 1,696,019 through nucleotide 1,705,056 of the Mycobacterium tuberculosis chromosome; (g) nucleotide 1,696,019 through nucleotide 1,705,808 of the Mycobacterium tuberculosis chromosome; (h) nucleotide 1,696,019 through nucleotide 1,706,631 of the Mycobacterium tuberculosis chromosome; or (i) nucleotide 1,696,019 through nucleotide 1,707,530 of the Mycobacterium tuberculosis chromosome.
 4. A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from: (a) nucleotide 1,696,441 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (b) nucleotide 1,697,420 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (c) nucleotide 1,699,892 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (d) nucleotide 1,701,088 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (e) nucleotide 1,702,588 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (f) nucleotide 1,704,091 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (g) nucleotide 1,705,056 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (h) nucleotide 1,705,784 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; (i) nucleotide 1,707,524 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome; or (j) nucleotide 1,706,593 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome.
 5. A purified polypeptide encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO: 1, wherein the polynucleotide consists of ORF6 between nucleotide 1,703,072 through nucleotide 1,704,091. 